scholarly journals Assessment of The Association of OCT3/4 With GLUT1 And CD105 In Oral Squamous Cell Carcinoma Using Dual Immunohistochemistry

2022 ◽  
Author(s):  
Samira Derakhshan ◽  
Nazanin Mahdavi ◽  
Neda Kardouni Khoozestani ◽  
Bita Nasr Esfahani ◽  
Forouzan Heidarian ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Tumor Progression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Late Stage ◽  
Cancer Type ◽  
Hypoxic Response ◽  
Glucose Transporter 1

Abstract Background: Oral squamous cell carcinoma (OSCC) is the most common cancer type affecting the oral and maxillofacial region. This study aimed to investigate the role of cancer stem cells (CSCs) in angiogenesis and hypoxic response in OSCC.Methods: This retrospective observational study evaluated 56 cases of OSCC using dual immunohistochemistry. Octamer-binding transcription factor 3/4 (OCT3/4) marker was used for evaluation of CSC activity. Glucose transporter 1 (GLUT1) marker was used to evaluate the hypoxic response and angiogenesis, while endoglin (CD105) was used to evaluate the late stage of angiogenesis and blood vessel formation.Results: Overexpression of both OCT3/4 and GLUT1 was noted in early stage of hypoxic response and angiogenesis in OSCC, indicating the important role of CSCs in this phase. However, despite higher expression of CD105, the expression of CSC markers was not significant in the late stage of tumor progression and angiogenesis.Conclusions: CSCs could play important roles in initial stages of tumor progression and angiogenesis. Further studies are required to discover other biomarkers, their roles, and associated pathways of CSCs in OSCC.

scholarly journals Differences in the expression of caveolin-1 isoforms in cancer-associated and normal fibroblasts of patients with oral squamous cell carcinoma

2021 ◽  
Author(s):  
S. Kaya ◽  
Nadine Wiesmann ◽  
J. Goldschmitt ◽  
M. Krüger ◽  
B. Al-Nawas ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Tumor Progression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Scaffolding Protein ◽  
Caveolin 1 ◽  
Tumor Stages

Abstract Objectives For many years, tumor development has been viewed as a cell-autonomous process; however, today we know that the tumor microenvironment (TME) and especially cancer-associated fibroblasts (CAFs) significantly contribute to tumor progression. Caveolin-1 (Cav-1) is a scaffolding protein which is involved in several cancer-associated processes as important component of the caveolae. Our goal was to shed light on the expression of the two different isoforms of Cav-1 in normal fibroblasts (NFs) and CAFs of patients with oral squamous cell carcinoma (OSCC). Materials and methods Fibroblasts from normal mucosa and CAFs were isolated and propagated in vitro. Gene expression of the different Cav-1 isoforms was assessed via quantitative real-time PCR (qPCR) and supplemented by protein expression analysis. Results We could show that the Cav-1β isoform is more highly expressed in NFs and CAFs compared to Cav-1α. Furthermore, the different Cav-1 isoforms tended to be differently expressed in different tumor stages. However, this trend could not be seen consistently, which is in line with the ambiguous role of Cav-1 in tumor progression described in literature. Western blotting furthermore revealed that NFs and CAFs might differ in the oligomerization profile of the Cav-1 protein. Conclusion These differences in expression of Cav-1 between NFs and CAFs of patients with OSCC confirm that the protein might play a role in tumor progression and is of interest for further analyses. Clinical relevance Our findings support a possible role of the two isoforms of Cav-1 in the malignant transformation of OSCC.

Aberrant Collagen Cross-linking in Human Oral Squamous Cell Carcinoma

2019 ◽  
Vol 98 (5) ◽  
pp. 517-525 ◽  
Author(s):  
T. Saito ◽  
K. Uzawa ◽  
M. Terajima ◽  
M. Shiiba ◽  
A.L. Amelio ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Tumor Progression ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Late Stage ◽  
Cross Linking ◽  
Key Enzymes ◽  
Cross Links ◽  
Collagen Cross Linking

Tumor progression is a complex process involving extracellular matrix (ECM) remodeling and stiffening. However, the mechanisms that govern these processes and their roles in tumor progression are still poorly understood. In this study, we performed bioinformatics, immunohistochemical, and biochemical analyses to examine if collagen cross-linking is associated with tumor stage and regional lymph node metastasis (RLNM) in oral squamous cell carcinoma (OSCC). We found that the genes encoding key enzymes for cross-linking are frequently overexpressed in oral, head, and neck cancers. Specifically, the enzymes lysyl hydroxylase 2 (LH2) or lysyl oxidase (LOX) and LOX-like 2 (LOXL2) were significantly upregulated in late-stage tumors and associated with poor patient prognosis. The protein levels of these enzymes in the primary human OSCC were also significantly increased in late-stage tumors and markedly elevated in the RLNM-positive tumors. Notably, while overall LOX/LOXL2-catalyzed collagen cross-links were enriched in late-stage and RLNM-positive tumors, LH2-mediated stable cross-links were significantly increased. To our knowledge, this is the first study to investigate the association of collagen cross-linking and expression of key enzymes regulating this process with OSCC stage. The data indicate a critical role for collagen cross-linking in OSCC tumor progression and metastasis, which may provide insights into development of novel therapeutic strategies to prevent OSCC progression.

The role of p300 in the tumor progression of oral squamous cell carcinoma

2014 ◽  
Vol 44 (3) ◽  
pp. 185-192 ◽  
Author(s):  
Young-Ah Cho ◽  
Ji-Soo Hong ◽  
Eun-Jin Choe ◽  
Hye-Jung Yoon ◽  
Seong-Doo Hong ◽  
...  
Keyword(s):  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Tumor Progression ◽  
Cell Carcinoma ◽  
Squamous Cell

Rediscussing the Role of Traditional Risk Factors in Young Adults With Oral Squamous Cell Carcinoma

2020 ◽  
Vol 40 (12) ◽  
pp. 6987-6995
Author(s):  
NORBERT NECKEL ◽  
MARCO MICHAEL ◽  
DANIEL TROELTZSCH ◽  
JONAS WÜSTER ◽  
STEFFEN KOERDT ◽  
...  
Keyword(s):  
Risk Factors ◽  
Squamous Cell Carcinoma ◽  
Young Adults ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Squamous Cell ◽  
Traditional Risk Factors

scholarly journals Role of viruses in oral squamous cell carcinoma

2012 ◽  
Vol 6 (2) ◽  
pp. 21 ◽  
Author(s):  
Rashmi Metgud ◽  
Madhusudan Astekar ◽  
Meenal Verma ◽  
Ashish Sharma
Keyword(s):  
Risk Factors ◽  
Squamous Cell Carcinoma ◽  
Oral Squamous Cell Carcinoma ◽  
Cell Carcinoma ◽  
Genetic Susceptibility ◽  
Squamous Cell ◽  
Oncogenic Viruses ◽  
Exact Role ◽  
Carcinogenic Effects

The etiology of oral squamous cell carcinoma (OSCC) is complex and involves many factors. The most clearly defined risk factors are smoking and alcohol, which substantially increase the risk of oral SCC. However, despite this clear association, a substantial proportion of patients develop OSCC without exposure to them, emphasizing the role of other risk factors such as genetic susceptibility and oncogenic viruses. Some viruses are strongly associated with OSCC while the association of others is less frequent and may depend on co-factors for their carcinogenic effects. Therefore, the exact role of viruses must be evaluated with care in order to improve the diagnosis and treatment of OSCC.

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