In Vivo Assessment of Burn Depth and Wound Healing Using a Handheld Terahertz Hyperspectral Scanner
Abstract The accuracy of clinical assessment in partial-thickness burn injuries has remained as low as 50-75%. Depending on the depth and environmental factors in the wound, such as reactive oxygen species, inflammation, and autophagy, partial-thickness burns can heal spontaneously or require surgical intervention. In this study, we demonstrate that Terahertz Time-Domain Spectral Imaging (THz-TDSI) is a promising tool for in vivo quantitative assessment of burn injuries. We used a novel handheld THz-TDSI scanner to characterize burn injuries in a porcine scald model with histopathological control. Prior work used THz reflectivity (representation of tissue hydration) as the only source of signal contrast. However, we used the spectral amplitude and the spectral slope of the terahertz electric field to distinguish the different severities of burns, suggesting that the energy loss due to electromagnetic scattering from skin constituents serves an additional metric to quantitatively assess burn injuries. Statistical analysis (n = 40) indicates that THz-TDSI can accurately differentiate between partial-thickness and full-thickness burn injuries (1-way ANOVA, p < 0.05) and monitor the healing process of partial thickness burns. THz-TDSI has the potential to improve burn care outcomes by helping surgeons to make objective decisions for early excision.