scholarly journals Expression Profile of Exosome-associated MicroRNA Derived from Human Red Blood Cell Suspensions During Storage: Predicting microRNA-1246 and microRNA-150-3p Exert Essential Roles in Transfusion-related Immunomodulation

2021 ◽  
Author(s):  
Yujie Kong ◽  
Xue Tian ◽  
Rui He ◽  
Chenyue Li ◽  
Haixia Xu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Target Genes ◽  
Mitogen Activated Protein Kinase ◽  
Ras Signaling ◽  
Qrt Pcr ◽  
Exosomal Mirnas ◽  
Storage Times ◽  
Functional Components

Abstract Background: Transfusion-related immunomodulation (TRIM) can be caused by exosomes and microRNA (miRNA) is one of the critical functional components in exosomes. This study intends to investigate the differences in the expression of exosomal miRNAs in red blood cell (RBC) suspensions at different storage times, also the potential functions related to TRIM of the abundant miRNAs. Methods: Twenty-five bags of RBC suspensions were selected randomly, and exosomes were separated by ultracentrifugation at different storage times. Isolated exosomes were identified by Nanoparticle Tracking Analysis (NTA), Transmission Electron Microscopy (TEM), and Western Blot (WB). Exosomal miRNA profiles were analyzed using genechip in 5 RBC suspension samples, and the miRNA expressions between different storage times were compared. For the statistically upregulated microRNAs, the bioinformation of their predicted target genes was analyzed. Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) was used to identify the miRNAs upregulated more than 10 folds at 5weeks storage time in 20 RBC suspension samples.Results: The detection of the gene chip showed most exosomal miRNAs were up-regulated as storage time increases. Compared to RBC suspensions stored for 1 week, that kept for 5 weeks had 539 differential miRNA expressions, among which 159 were significantly different (P<0.05) and 148 (93.08%) were up-regulated. For the bioinformatic analysis, significant immunoregulatory annotations related to thyroid hormone, mitogen-activated protein kinase (MAPK), focal adhesion, and ras signaling pathway were found. The top 17 miRNAs were validated by qRT-PCR, and the results showed miRNA-1246 and miRNA-150-3p were the most significantly enriched miRNAs (more than 150 folds during 5weeks storage).Conclusions: As storage time increased, various exosomal miRNAs in RBC suspensions accumulated and involved multiple immuno-signaling pathways. The predominantly accumulated miRNA-1246 and miRNA-150-3p were confirmed to participate in pro-inflammation responses and immune-regulation, which might exert essential roles in TRIM.

scholarly journals The accumulation of exosome-associated microRNA-1246 and microRNA-150-3p in human red blood cell suspensions

2021 ◽  
Vol 19 (1) ◽  
Author(s):  
Yujie Kong ◽  
Xue Tian ◽  
Rui He ◽  
Chenyue Li ◽  
Haixia Xu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Signaling Pathways ◽  
Red Blood Cell ◽  
Expression Profiles ◽  
Storage Period ◽  
Gene Chip ◽  
Ras Signaling ◽  
Qrt Pcr ◽  
Exosomal Mirnas ◽  
Exosomal Mirna

Abstract Background Transfusion-related immunomodulation (TRIM) can be caused by exosomes, in which case, microRNAs (miRNAs) are one critical factor impacting exosome behavior. This study aims to investigate and analyze the expression profiles of exosomal miRNA in red blood cell (RBC) suspensions during storage and to identify potential TRIM-related miRNAs as well as their potential functions. Methods A total of 25 packs of RBC suspensions were randomly collected. Exosome were extracted by ultracentrifugation and then identified and characterized by nanoparticle tracking analysis (NTA), transmission electron microscopy (TEM) and western blot (WB). Exosomal miRNA profiles were acquired using gene chips in five packs on week 1 and week 5. The expression data were compared from the two time points identifying accumulated miRNAs with statistical significance and their predicted targeting genes were analyzed. Based on the gene chip results, quantitative reverse transcription-polymerase chain reactions (qRT-PCR) were performed to verify miRNA accumulation in the rest 20 packs sampling on week 1, 3 and 5. Results Gene chip analysis revealed that most exosomal miRNAs were enriched as the storage period progressed. Compared to samples from week 1, week 5 samples exhibited a total of 539 differential miRNA expressions, among which, 159 were statistically significant (P < 0.05) and 148 (93.08%) were accumulated. In the bioinformatics functional analysis, significant immunoregulatory annotations related to the thyroid hormone, mitogen-activated protein kinase (MAPK), focal adhesion and RAS signaling pathways were identified. The top 17 differential expression miRNAs were validated by qRT-PCR. The results confirmed that all the 17 miRNAs were accumulated with increasing storage time. In particular, miRNA-1246 and miRNA-150-3p were the most enriched strands by more than 150-folds in the 5-week storage period. Conclusions As storage progressed, numerous exosomal miRNAs accumulated in the RBC suspensions, which are informatically connected to multiple immuno-signaling pathways. MiRNA-1246 and miRNA-150-3p may be essential mediators impacting the immunoregulation functions of exosomes in RBC suspensions, considering their significant accumulating scales. Further research should therefore focus on the relationship between these miRNAs and TRIM.

scholarly journals MicroRNA Profiling of Exosomes Derived from Red Blood Cell Units: Implications in Transfusion-Related Immunomodulation

2019 ◽  
Vol 2019 ◽  
pp. 1-10 ◽  
Author(s):  
Haobo Huang ◽  
Jinfeng Zhu ◽  
Liping Fan ◽  
Qiuyan Lin ◽  
Danhui Fu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Bioinformatic Analysis ◽  
Mirna Microarray ◽  
Qrt Pcr ◽  
Transmission Electron ◽  
Mean Size ◽  
The Mean ◽  
Polymerase Chain

Purpose. To elucidate the microRNAs existent in exosomes derived from stored red blood cell (RBC) unit and their potential function. Materials and Methods. Exosomes were isolated from the supernatant derived from stored RBC units by sequential centrifugation. Isolated exosomes were characterized by TEM (transmission electron microscopy), western blotting, and DLS (dynamic light scattering). MicroRNA (miRNA) microarray was performed to detect the expression of miRNAs in 3 exosome samples. Results revealed miRNAs that were simultaneously expressed in the 3 exosome samples and were previously reported to exist in mature RBCs. Functions and potential pathways of some detected miRNAs were illustrated by bioinformatic analysis. Validation of the top 3 abundant miRNAs was carried out by qRT-PCR (quantitative reverse transcription‐polymerase chain reaction). Results. TEM and DLS revealed the mean size of the exosomes (RBC-derived) as 64.08 nm. These exosomes exhibited higher abundance of short RNA than the long RNA. 78 miRNAs were simultaneously detected in 3 exosome samples and mature RBCs. Several biological processes might be impacted by these miRNAs, through their target gene(s) enriched in a particular signalling pathway. The top 3 (abundant) miRNAs detected were as follows: miR-125b-5p, miR-4454, and miR-451a. qRT-PCR revealed higher abundance of miR-451a than others. Only miR-4454 and miR-451a abundance tended to increase with increasing storage time. Conclusion. Exosomes derived from stored RBC units possessed multiple miRNAs and, hence, could serve various functions. The function of exosomes (RBC-derived) might be implemented partly by the predominantly enriched miR-451a.

The Effects of Red Blood Cell Storage Time on Nitric Oxide and Nitrite-dependent Signaling

2010 ◽  
Vol 49 ◽  
pp. S30
Author(s):  
Ryan Daniel Stapley ◽  
Dario A Vitturi ◽  
Cilina Rodriguez ◽  
Rakesh P Patel
Keyword(s):  
Nitric Oxide ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Cell Storage

scholarly journals Effect of red blood cell storage time on markers of hemolysis and inflammation in transfused very low birth weight infants

2017 ◽  
Vol 82 (6) ◽  
pp. 964-969 ◽  
Author(s):  
Tamara G Kalhan ◽  
David A Bateman ◽  
Rakhee M Bowker ◽  
Eldad A Hod ◽  
Sudha Kashyap
Keyword(s):  
Birth Weight ◽  
Low Birth Weight ◽  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Very Low Birth Weight ◽  
Low Birth Weight Infants ◽  
Cell Storage

Does the storage time of human blood have any impact on the outcome of the red blood cell lysis cytotoxicity test?

2006 ◽  
Vol 164 ◽  
pp. S113
Author(s):  
Jens Lichtenberg ◽  
Inge Birgit Jørgensen ◽  
Ninna Willestofte Berg
Keyword(s):  
Blood Cell ◽  
Human Blood ◽  
Red Blood Cell ◽  
Storage Time ◽  
Cell Lysis ◽  
Cytotoxicity Test

Red blood cell storage time and the outcome after coronary surgery

2015 ◽  
Vol 197 (1) ◽  
pp. 58-64 ◽  
Author(s):  
Eeva-Maija Kinnunen ◽  
Ludovico Sabatelli ◽  
Tatu Juvonen ◽  
Fausto Biancari
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Coronary Surgery ◽  
Cell Storage

scholarly journals Heterogeneity of blood processing and storage additives in different centers impacts stored red blood cell metabolism as much as storage time: lessons from REDS‐III—Omics

Transfusion ◽  
2018 ◽  
Vol 59 (1) ◽  
pp. 89-100 ◽  
Author(s):  
Angelo D'Alessandro ◽  
Rachel Culp‐Hill ◽  
Julie A. Reisz ◽  
Mikayla Anderson ◽  
Xiaoyun Fu ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Cell Metabolism ◽  
Blood Processing ◽  
Processing And Storage ◽  
And Storage

scholarly journals Reduction of exposure to plasticizers in stored red blood cell units

Perfusion ◽  
2019 ◽  
Vol 35 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Frank Münch ◽  
Thomas Göen ◽  
Robert Zimmermann ◽  
Werner Adler ◽  
Ariawan Purbojo ◽  
...  
Keyword(s):  
Blood Cell ◽  
Red Blood Cell ◽  
Storage Time ◽  
Time Range ◽  
Cell Salvage ◽  
Chemical Contamination ◽  
Daily Lives ◽  
Duration Of Storage ◽  
Before And After ◽  
Ethylhexyl Phthalate

Introduction: Plastic can be toxic and hazardous to an organism’s health, but it is being widely used in our daily lives. Di-2-ethylhexyl-phthalate is the most common plasticizer in medical devices made of polyvinylchloride and is commonly found in soft bags storing red blood cell units. Di-2-ethylhexyl-phthalate and its degradation product mono-2-ethylhexyl-phthalate can migrate into human body fluids, for example, blood and tissues. The aim of the study was to assess the concentration of plasticizers in red blood cell units according to storage time and after mechanical rinsing using a cell salvage device. Methods: Levels of di-2-ethylhexyl-phthalate and mono-2-ethylhexyl-phthalate were analysed in 50 unwashed red blood cell units using liquid chromatography coupled with tandem mass spectrometry. In addition, phthalate concentrations were measured before and after mechanical rinsing in six more washed red blood cell units with storage times ranging between 36 and 56 days. A linear regression model was determined by the daily increase of di-2-ethylhexyl-phthalate and mono-2-ethylhexyl-phthalate in the stored red blood cell units subject to their storage time (range = 4-38 days), and the effect of mechanical rinsing on their phthalate concentration was calculated. Results: A linear correlation was found between storage time of unwashed red blood cell units and the concentration of di-2-ethylhexyl-phthalate (p < 0.001) or mono-2-ethylhexyl-phthalate (p < 0.001). Stored red blood cell units older than 14 days had significantly higher concentrations of both contaminants than red blood cell units of shorter storage time (p < 0.001). Mechanical rinsing in washed red blood cell units attained a reduction in the di-2-ethylhexyl-phthalate and mono-2-ethylhexyl-phthalate concentration by a median of 53% (range = 18-68%; p = 0.031) and 87% (range = 68-96%; p = 0.031), respectively. Conclusion: Leaching of di-2-ethylhexyl-phthalate and mono-2-ethylhexyl-phthalate into red blood cell units depends on the duration of storage time. Plasticizers can be significantly reduced by mechanical rinsing using cell salvage devices, and thus, red blood cell units can be regenerated with respect to chemical contamination.

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