scholarly journals Radial Extracorporeal Shock Wave Reduce Myogenic Contracture and Muscle Atrophy via inhibiting NF-κB/HIF-1α Signaling Pathway in Rabbit

2021 ◽  
Author(s):  
Feng Wang ◽  
Wen Li ◽  
Yun Zhou ◽  
Peng Peng Huang
Keyword(s):  
Shock Wave ◽  
Knee Joint ◽  
Signaling Pathway ◽  
Muscle Atrophy ◽  
Biological Effects ◽  
Left Knee ◽  
Myogenic Regulatory Factors ◽  
Protein Levels ◽  
Extracorporeal Shock Wave ◽  
Time Point

Abstract BackgroundRecent studies have shown that radial extracorporeal shock wave therapy (rESWT) modulates viability and gene expression of human skeletal muscle cells in vitro. However, the underlying biological effects and mechanisms of rESWT on muscle atrophy and myogenic contracture in animal mode have not yet been investigated.ObjectiveTo investigate the underlying biological effects and mechanisms of rESWT on myogenic contracture and muscle atrophy in a rabbit model of extending knee joint contracture.MethodsForty rabbits were randomly divided into eight groups. In group Control, the left knee joint was not fixed. In group I-4w, the left knee joint was only fixed for four weeks. In groups SR-1w, SR-2w, and SR-4w, the left knee joint was fixed for four weeks before the rabbits underwent one, two, and four weeks of self-recovery, respectively. In groups rESWT-1w, rESWT-2w, and rESWT-4w, the left knee joint was fixed for four weeks before the rabbits underwent one, two, and four weeks of rESWT, respectively. The degree of myogenic contracture was measured, the cross-sectional area and key protein levels for NF-κB/HIF-1α signaling pathway and myogenic regulatory factors of the rectus femoris were evaluated.ResultsDuring the recovery period, biological findings in this study showed that the levels of myogenic contracture and muscle atrophy were milder in group rESWT by compared with group SR at the same time point. Molecular biological analysis in the present study showed that MyoD protein levels in the group rESWT was significantly higher than those in the group SR, and importantly, phospho-NF-κB p65 and HIF-1α protein levels in the group rESWT were significantly lower than those in the group SR at the same time point.ConclusionsOur study demonstrates that rESWT has the potential to reduce myogenic contracture and muscle atrophy after long-term immobilization in animal model. It is a possible mechanism that changing the low oxygen environment in skeletal muscle through rESWT may inhibit activation of NF-κB/HIF-1α signaling pathway, thereby enhancing the expression of myogenic regulatory factors.

scholarly journals Role of the PERK-eIF2α-CHOP Signaling Pathway in the Effect of Needle Knife Therapy on Knee Joint Chondrocyte Apoptosis

2019 ◽  
Vol 2019 ◽  
pp. 1-7 ◽  
Author(s):  
Yong-hui Yang ◽  
Tian-hao Liu ◽  
Li-da Zhang ◽  
Zhu-yue Chen ◽  
Xiao-shuang Huang
Keyword(s):  
Knee Joint ◽  
Signaling Pathway ◽  
Ultrastructural Changes ◽  
Chondrocyte Apoptosis ◽  
Mrna Levels ◽  
Model Group ◽  
Protein Levels ◽  
Transmission Electron ◽  
Starting Point ◽  
Needle Knife

Needle knife therapy, a form of acupuncture and moxibustion, has been widely used in the clinical treatment of knee osteoarthritis (KOA). However, the mechanism is not clear. Therefore, we studied the mechanisms of action of needle knife intervention on KOA in rabbits, with the PERK-eIF2α-CHOP pathway as a starting point, in order to determine the mechanism underlying knee joint chondrocyte apoptosis. Apoptosis and ultrastructural changes in the articular cartilage were examined by pathological study and transmission electron microscopy, and PERK, eIF2α, and CHOP mRNA and protein levels were detected by qRT-PCR and western blot, respectively. PERK, eIF2α, and CHOP protein levels were significantly higher in the model group than in the normal group (P< 0.01) and were considerably downregulated in the needle knife and the medicine groups compared to the model group (P< 0.01). The eIF2α, p-eIF2α, and CHOP protein levels were not significantly different between the needle knife and medicine groups. The PERK, eIF2α, and CHOP mRNA levels in the drug group were higher than those in the needle knife group (P< 0.01). Needle knife therapy can regulate PERK-eIF2α-CHOP signaling pathway, which could be one of the mechanisms by which it affects chondrocyte apoptosis in KOA rabbits.

Prospects for Development of Shock Wave Therapy

2008 ◽  
pp. 1125-1129
Author(s):  
B. A. Garilevich ◽  
Y. V. Kudryavtsev ◽  
Y. V. Olefir ◽  
Y. V. Andrianov ◽  
A. E. Rotov
Keyword(s):  
Shock Wave ◽  
Shock Waves ◽  
Shock Wave Lithotripsy ◽  
Biological Effects ◽  
Medical Systems ◽  
Extracorporeal Shock Wave ◽  
Shock Wave Generation ◽  
The World ◽  
Physical Effects ◽  
The Given

Nowadays the world medical practice widely uses the method of extracorporeal shock wave lithotripsy for treatment of patients with urolithiasis bay means of pulverizing action of focused shock waves (FSW). In the early 1980s in Germany, the Dornier Medical Systems Company fabricated the first lithotriptor based on the electrohydraulic principle of shock wave generation. By 1988, practically all countries had applied lithotriptors developed by Dornier Medical Systems. Later, more than 15 countries started to fabricate their own lithotriptors, which use various methods of FSW generation, such as electrohydraulic, electromagnetic, and piezoelectric. These countries were Germany, USA, Russia, China, Italy, France, Israel, Turkey, Slovakia, Poland, Austria, Sweden, and so forth. However, the given method of the physical effects can be used not only for the extracorporeal fragmentation of calculi. The biological effects of shock waves presume a perspective of their use for treatment and aftertreatment of the patients with other disorders and pathological conditions using their stimulatory and inhibitory actions.

scholarly journals Low-frequency Electrical Stimulation Alleviates Immobilization-evoked Disuse Muscle Atrophy via Repressing Autophagy in Skeletal Muscle of Rabbits

2021 ◽  
Author(s):  
A-Ying Liu ◽  
Quan-Bing Zhang ◽  
Hua-Long Zhu ◽  
Yong-Wei Xiong ◽  
Feng Wang ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Electrical Stimulation ◽  
Knee Joint ◽  
Muscle Atrophy ◽  
Rabbit Model ◽  
Low Frequency ◽  
Cross Sectional ◽  
Disuse Muscle Atrophy ◽  
Joint Range ◽  
Time Point

Abstract Objective: The present study was to investigate the effect of low-frequency electrical stimulation on disuse muscle atrophy and its mechanism in a rabbit model of extending knee joint contracture.Methods: This study designed two experiments. In the time-point experiment, 24 rabbits were randomly divided into Control 1(Ctrl1), immobilization for 2 weeks (I-2), I-4, and I-6 groups. In the intervention experiment, 24 rabbits were also randomly divided into Control 2 (Ctrl2), electrical stimulation (ES), natural recovery (NR) and electrical stimulation treatment (EST) groups. All intervention effects were assessed by evaluating the knee joint range of motion (ROM), cross-sectional area (CSA) of muscle and the expression of autophagy-related proteins.Results: Time-point experiment showed that immobilization reduced knee ROM, muscle CSA, and activated autophagy in skeletal muscle. Levels of four autophagic proteins including p-mTOR, Atg7, p62 and LC3B-II, were significantly elevated in the skeletal muscle of I-4 group. The intervention experiment further presented that LFES significantly improved the immobilization-induced ROM and CSA reduction. Additionally, LFES significantly reversed autophagy activation of skeletal muscle caused by immobilization.Conclusions: Low-frequency electrical stimulation alleviates immobilization-evoked disuse muscle atrophy maybe via inhibiting autophagy in skeletal muscle of rabbits.

scholarly journals Outcome of Extracorporeal Shock Wave Therapy for Insertional Achilles Tendinopathy with and without Haglund’s Deformity

2016 ◽  
Vol 2016 ◽  
pp. 1-6 ◽  
Author(s):  
Ziying Wu ◽  
Wei Yao ◽  
Shiyi Chen ◽  
Yunxia Li
Keyword(s):  
Shock Wave ◽  
Extracorporeal Shock Wave Therapy ◽  
Likert Scale ◽  
Achilles Tendinopathy ◽  
Shock Wave Therapy ◽  
Extracorporeal Shock Wave ◽  
Haglund’S Deformity ◽  
Insertional Achilles Tendinopathy ◽  
Time Point

Purpose.To compare the results of extracorporeal shock wave therapy (ESWT) for insertional Achilles tendinopathy (IAT) with or without Haglund’s deformity.Methods.Between September 2014 and May 2015, all patients who underwent ESWT were retrospectively enrolled in this study. A total of 67 patients were available for follow-up and assigned into nondeformtiy group (n=37) and deformtiy group (n=30). Clinical outcomes were evaluated by VISA-A Score and 6-point Likert scale.Results.The VISA-A score increased in both groups, from49.57±9.98at baseline to83.86±8.59at14.5±7.2months after treatment in nondeformity group (P<0.001) and from48.70±9.38at baseline to67.78±11.35at15.3±6.7months after treatment in deformity group (P<0.001). However, there was a greater improvement in VISA-A Score for the nondeformity group compared with deformity group (P=0.005). For the 6-point Likert scale, there were decreases from3.92±0.80at baseline to1.57±0.73at the follow-up time point in nondeformity group (P<0.001) and from4.0±0.76at baseline to2.37±1.03at the follow-up time point in deformity group (P<0.001). There was no significant difference in improvement of the 6-point Likert scale between both groups (P=0.062).Conclusions.ESWT resulted in greater clinical outcomes in patients without Haglund’s deformity compared with patients with Haglund’s deformity.

Sign in / Sign up

Export Citation Format

Share Document