scholarly journals Sodium Nitroprusside Enhances Biomass And Gymnemic Acids Production In Cell Suspension of Gymnema Sylvestre (Retz.) R.Br. Ex. Sm.

2021 ◽  
Author(s):  
Ganesan Mahendran ◽  
Deepak Kumar ◽  
Sanjeet Kumar Verma ◽  
Ashish Chandran ◽  
Zafar Iqbal Warsi ◽  
...  
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Sodium Nitroprusside ◽  
Cell Suspension Culture ◽  
Large Scale ◽  
Gymnema Sylvestre ◽  
Scale Production ◽  
Produce Cell ◽  
Triterpenoid Saponins ◽  
Gymnemic Acid

Abstract Gymnema sylvestre (Retz.) R.Br. ex Sm. is widely used as an efficient Ayurvedic traditional medicine for the treatment of diabetes. Phytochemical investigations of this plant showed gymnemic acids (a group of triterpenoid saponins) as the main active components. The present study aims to investigate the effectiveness of sodium nitroprusside (SNP) treatment for enhancement of cell suspension culture biomass and to evaluate their deacylgymnemic acid, gymnemagenin, gymnemic acid IV and gymnemic acid XVII contents of G. sylvestre. Callus was obtained from in vitro derived leaves of G. sylvestre on MS medium fortified with 3.0 mg/L 2, 4-D (2, 4-dichlorophenoxyacetic acid) and 2.0 mg/L Kn (Kinetin), and the same were used further to produce cell suspension cultures. Cell suspensions were exposed to different concentrations of SNP (5, 10, 20 and 40 µM) and data were collected at 20, 30 and 40 days. Out of the tested concentrations, 20 µM SNP had the highest level of cell culture growth (398.94 ± 8.32 g/L FCW and 40.00 ± 0.75 g/L DCW) on 40-day as compared to control. High-performance liquid chromatography analysis showed that maximum accumulation of deacylgymnemic acid (5.51 mg/g DCW), gymnemagenin (2.80 mg/g DCW) and gymnemic acid XVII (2.08 mg/g DCW) in 20 µM SNP treatment which is (13.43, 13.86 and17.33 folds) higher than the respective control at 40 days exposure. This research suggests that G. sylvestre cell suspension culture with optimal SNP elicitation treatment could be used as a good strategy for the large-scale production of these secondary metabolites at the industrial level.

scholarly journals Engineering Considerations to Produce Bioactive Compounds from Plant Cell Suspension Culture in Bioreactors

Plants ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 2762
Author(s):  
Elizabeth Alejandra Motolinía-Alcántara ◽  
Carlos Omar Castillo-Araiza ◽  
Mario Rodríguez-Monroy ◽  
Angélica Román-Guerrero ◽  
Francisco Cruz-Sosa
Keyword(s):  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Large Scale ◽  
Scaling Up ◽  
Operating Conditions ◽  
Scale Production ◽  
Plant Cell Suspension Culture ◽  
Plant Cell Suspension ◽  
Large Scale Production

The large-scale production of plant-derived secondary metabolites (PDSM) in bioreactors to meet the increasing demand for bioactive compounds for the treatment and prevention of degenerative diseases is nowadays considered an engineering challenge due to the large number of operational factors that need to be considered during their design and scale-up. The plant cell suspension culture (CSC) has presented numerous benefits over other technologies, such as the conventional whole-plant extraction, not only for avoiding the overexploitation of plant species, but also for achieving better yields and having excellent scaling-up attributes. The selection of the bioreactor configuration depends on intrinsic cell culture properties and engineering considerations related to the effect of operating conditions on thermodynamics, kinetics, and transport phenomena, which together are essential for accomplishing the large-scale production of PDSM. To this end, this review, firstly, provides a comprehensive appraisement of PDSM, essentially those with demonstrated importance and utilization in pharmaceutical industries. Then, special attention is given to PDSM obtained out of CSC. Finally, engineering aspects related to the bioreactor configuration for CSC stating the effect of the operating conditions on kinetics and transport phenomena and, hence, on the cell viability and production of PDSM are presented accordingly. The engineering analysis of the reviewed bioreactor configurations for CSC will pave the way for future research focused on their scaling up, to produce high value-added PDSM.

scholarly journals TINJAUAN Penggunaan Suspensi Sel dalam Kultur In Vitro

2016 ◽  
Vol 5 (2) ◽  
pp. 84 ◽  
Author(s):  
Sri Hutami
Keyword(s):  
Secondary Metabolites ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Mass Production ◽  
Large Scale ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Cell Physiology

<p>Cell suspension culture could be defined as a<br />process that allows rapidly dividing homogenous suspension<br />of cells to grow in liquid nutrient media. There are two main<br />types of suspension cultures: (1) Batch cultures in which<br />cells are nurtured in a fixed volume of medium until growth<br />ceases and (2) Continuous cultures in which cell growth is<br />maintained by continuous replenishment of sterile nutrient<br />media. Plant cell suspension cultures are mostly used for the<br />biochemical investigation of cell physiology, growth, metabolism,<br />protoplast fusion, transformation and for large scale<br />production of seed by bioreactor and production of secondary<br />metabolites. Contamination is one of the largest problems<br />when dealing with cell cultures. Differences between<br />the products of cell suspension culture and whole plant are<br />frequently observed. These phenomena’s may be resulted<br />from lack of differentiation and organization and cell cultureinduced<br />variation. Utilization of cell suspension culture in<br />Indonesia is still limited, some of them for mass production<br />of plantation seed with bioreactor system and for production<br />of secondary metabolites. The success of this study give the<br />opportunity for mass production of seeds from other plants<br />and also production of secondary metabolites.</p>

Sodium nitroprusside enhances biomass and gymnemic acids production in cell suspension of Gymnema sylvestre (Retz.) R.Br. ex. Sm.

2021 ◽  
Author(s):  
Ganesan Mahendran ◽  
Deepak Kumar ◽  
Sanjeet Kumar Verma ◽  
Ashish Chandran ◽  
Zafar Iqbal Warsi ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Sodium Nitroprusside ◽  
Gymnema Sylvestre

scholarly journals Citrus Cell Suspension Culture Establishment, Maintenance, Efficient Transformation and Regeneration to Complete Transgenic Plant

Plants ◽  
2021 ◽  
Vol 10 (4) ◽  
pp. 664
Author(s):  
M. Moniruzzaman ◽  
Yun Zhong ◽  
Zhifeng Huang ◽  
Huaxue Yan ◽  
Lv Yuanda ◽  
...  
Keyword(s):  
Transgenic Plants ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Culture System ◽  
Transformation Rate ◽  
Suspension Cell Culture ◽  
Citrus Reticulata ◽  
Agrobacterium Mediated Transformation ◽  
Solid Media

Agrobacterium-mediated transformation of epicotyl segment has been used in Citrus transgenic studies. The approach suffers, however, from limitations such as occasionally seed unavailability, the low transformation efficiency of juvenile tissues and the high frequency of chimeric plants. Therefore, a suspension cell culture system was established and used to generate transgenic plants in this study to overcome the shortcomings. The embryonic calli were successfully developed from undeveloped ovules of the three cultivars used in this study, “Sweet orange”-Egyptian cultivar (Citrus sinensis), “Shatangju” (Citrus reticulata) and “W. Murcott” (Citrus reticulata), on three different solid media. Effects of media, genotypes and ages of ovules on the induction of embryonic calli were also investigated. The result showed that the ovules’ age interferes with the callus production more significantly than media and genotypes. The 8 to 10 week-old ovules were found to be the best materials. A cell suspension culture system was established in an H+H liquid medium. Transgenic plants were obtained from Agrobacterium-mediated transformation of cell suspension as long as eight weeks subculture intervals. A high transformation rate (~35%) was achieved by using our systems, confirming BASTA selection and later on by PCR confirmation. The results demonstrated that transformation of cell suspension should be more useful for the generation of non-chimeric transgenic Citrus plants. It was also shown that our cell suspension culture procedure was efficient in maintaining the vigor and regeneration potential of the cells.

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