scholarly journals Candida tropicalis distribution and drug resistance correlated to ERG11 and UPC2 expression

2020 ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mrna Expression ◽  
Candida Tropicalis ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Airway Secretion ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Mrna Expression Levels

Abstract Objective: This study is to analyze the distribution characteristics and drug resistance of Candida tropicalis, and the relationship between ERG11 and UPC2 expression and the resistance to azole.Methods: The Candida tropicalis were cultured and identified by the Sabouraud Agar Medium, CHROM Agar Candida and France Bio-Mérieux ATB test. The total RNA of the collected strains was extracted, and the ERG11 and UPC2 mRNA expression levels were analyzed by the quantitative real-time PCR. Results: Totally 2872 clinical isolates of Candida, including 319 strains of Candida tropicalis were analyzed herein, which were mainly distributed in the Departments of Respiratory Medicine and ICU. The specimen type was mainly based on the airway secretion, and the detection trend in four years was mainly related to the type of the departments and specimens. The resistance rates of Candida tropicalis to fluconazole, itraconazole and voriconazole have been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole resistant group were significantly higher than the sensitive group. In addition, there was a significant linear positive correlation between these two genes in the fluconazole resistant group.Conclusions: Over-expression of ERG11 and UPC2 genes in Candida tropicalis could increase the resistance to antifungal drugs. The routine detection of ERG11 and UPC2 for high-risk patients in the key departments would provide theoretical basis for the rational application of antifungal drugs.

scholarly journals Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression

2020 ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Drug Resistance ◽  
Mrna Expression ◽  
Candida Tropicalis ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Positive Linear Correlation ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Mrna Expression Levels

Abstract Objective This study analyzed the distribution and drug resistance of Candida tropicalis ( C. tropicalis ) and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. Methods C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. Results In total, 2872 clinical isolates of Candida , including 319 strains of C. tropicalis , were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the sensitive group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. Conclusions Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.

scholarly journals Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression

2021 ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Candida Tropicalis ◽  
Drug Susceptibility ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Opportunistic Pathogenic ◽  
Mrna Expression Levels

Abstract Background: Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents.Methods: C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. Results: In total, 2872 clinical isolates of Candida, including 319 strains of C. tropicalis, were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group.Conclusions: Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.

scholarly journals Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression

2021 ◽  
Vol 10 (1) ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Candida Tropicalis ◽  
Drug Susceptibility ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Opportunistic Pathogenic ◽  
Mrna Expression Levels

Abstract Background Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. Methods C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. Results In total, 2872 clinical isolates of Candida, including 319 strains of C. tropicalis, were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. Conclusions Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.

scholarly journals Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression

2020 ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Candida Tropicalis ◽  
Drug Susceptibility ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Positive Linear Correlation ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Mrna Expression Levels

Abstract Objective This study analyzed the distribution and drug susceptibility of Candida tropicalis ( C. tropicalis ) and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents. Methods C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR. Results In total, 2872 clinical isolates of Candida , including 319 strains of C. tropicalis , were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the sensitive group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group. Conclusions Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.

scholarly journals Candida tropicalis distribution and drug resistance is correlated with ERG11 and UPC2 expression

2020 ◽  
Author(s):  
Dan Wang ◽  
Na An ◽  
Yuwei Yang ◽  
Xianggui Yang ◽  
Yingzi Fan ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Candida Tropicalis ◽  
Drug Susceptibility ◽  
Antifungal Drugs ◽  
Expression Levels ◽  
Sabouraud Agar ◽  
Fluconazole Resistant ◽  
Resistant Group ◽  
Opportunistic Pathogenic ◽  
Mrna Expression Levels

Abstract Background: Candida tropicalis (C. tropicalis) is an important opportunistic pathogenic Candida species that can cause nosocomial infection. In this study, we analyzed the distribution and drug susceptibility of C. tropicalis and the relationship between ERG11 and UPC2 expression and resistance to azole antifungal agents.Methods: C. tropicalis was cultured and identified by Sabouraud Agar Medium, CHROM Agar Candida and ATB tests (Bio-Mérieux, France). Total RNA was extracted from the collected strains, and the ERG11 and UPC2 mRNA expression levels were analyzed by quantitative real-time PCR.Results: In total, 2872 clinical isolates of Candida, including 319 strains of C. tropicalis, were analyzed herein; they were mainly obtained from the Departments of Respiratory Medicine and ICU. The strains were predominantly isolated from airway secretion samples, and the detection trend in four years was mainly related to the type of department and specimens. The resistance rates of C. tropicalis to fluconazole, itraconazole and voriconazole had been increasing year by year. The mRNA expression levels of ERG11 and UPC2 in the fluconazole-resistant group were significantly higher than they were in the susceptible group. In addition, there was a significant positive linear correlation between these two genes in the fluconazole-resistant group.Conclusions: Overexpression of the ERG11 and UPC2 genes in C. tropicalis could increase resistance to azole antifungal drugs. The routine testing for ERG11 and UPC2 in high-risk patients in key departments would provide a theoretical basis for the rational application of azole antifungal drugs.

scholarly journals The role of CXCR3 and its ligands expression in Brucellar spondylitis

2020 ◽  
Vol 21 (1) ◽  
Author(s):  
Xin Hu ◽  
Xiaoqian Shang ◽  
Liang Wang ◽  
Jiahui Fan ◽  
Yue Wang ◽  
...  
Keyword(s):  
Protein Expression ◽  
Mrna Expression ◽  
Mononuclear Cells ◽  
Healthy Controls ◽  
Inflammatory Infiltration ◽  
Peripheral Blood Mononuclear ◽  
Expression Levels ◽  
Inflammatory Damage ◽  
Ifn Γ ◽  
Mrna Expression Levels

Abstract Aim Brucellar spondylitis (BS) is one of the most serious complications of brucellosis. CXCR3 is closely related to the severity of disease infection. This research aimed to study the degree of BS inflammatory damage through analyzing the expression levels of CXCR3 and its ligands (CXCL9 and CXCL10) in patients with BS. Methods A total of 29 BS patients and 15 healthy controls were enrolled. Real-Time PCR was used to detect the mRNA expression levels of IFN-γ, CXCR3, CXCL9 and CXCL10 in peripheral blood mononuclear cells (PBMCs) of BS patients and healthy controls. Hematoxylin-Eosin staining was used to show the pathological changes in BS lesion tissues. Immunohistochemistry staining was used to show the protein expression levels of Brucella-Ab, IFN-γ, CXCR3, CXCL9 and CXCL10 in BS lesion tissues. At the same time, ELISA was used to detect the serum levels of IFN-γ, CXCL9 CXCL10 and autoantibodies against CXCR3 in patients with BS. Results In lesion tissue of BS patients, it showed necrosis of cartilage, acute or chronic inflammatory infiltration. Brucella-Ab protein was abundantly expressed in close lesion tissue. And the protein expression levels of IFN-γ, CXCR3 and CXCL10 were highly expressed in close lesion tissue and serum of BS patients. At the same time, the mRNA expression levels of IFN-γ, CXCR3 and CXCL10 in PBMCs of BS patients were significantly higher than those in controls. Conclusion In our research, the expression levels of IFN-γ, CXCR3 and its ligands were significantly higher than those in controls. It suggested that high expression levels of IFN-γ, CXCR3 and its ligands indicated a serious inflammatory damage in patients with BS.

Unilateral labyrinthectomy induced changes on GDNF receptor complex proteins in the rat medial vestibular nuclei

2007 ◽  
Vol 16 (4-5) ◽  
pp. 171-177
Author(s):  
Adrian Lozada ◽  
Kaj Karlstedt ◽  
Pertti Panula ◽  
Antti A. Aarnisalo
Keyword(s):  
Mrna Expression ◽  
Vestibular Nucleus ◽  
Medial Vestibular Nucleus ◽  
Receptor Complex ◽  
Trophic Effect ◽  
Expression Levels ◽  
Protein Levels ◽  
Unilateral Labyrinthectomy ◽  
Ganglion Neurons ◽  
Mrna Expression Levels

In the auditory periphery, GDNF has been shown to have a trophic effect to spiral ganglion neurons, both during development and in adult animals. We have studied the effect of unilateral labyrinthectomy (UL) on protein levels and expression of GDNF multicomponent receptor complex: the ret tyrosine kinase and coreceptor GFRα-1 in the medial vestibular nucleus of the adult rat. GFRα-1 protein levels display an increasing trend in ipsilateral medial vestibular nucleus culminating at 48 h post UL. On the other hand, GFRα-1 mRNA expression levels in ipsi- and contralateral medial vestibular nucleus show a steadily decreasing trend that is significant at 1 week post-lesion. Protein levels for c-Ret isoforms also show an initial bilateral decreasing trend that ceases at 48 h in ipsilateral medial vestibular nucleus but persists on the contralateral side. c-Ret mRNA expression levels show a significant decrease at 4 h post UL followed by another significant decrease 1 week post UL. Our data would suggest that neurotrophins belonging to the GDNF family are involved in this model of post-lesional CNS plasticity.

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