Enhanced Saccharfication of Pomegranate Peel by Trichoderma Reesei and Statiscal Optimization of Medium Composition by Response Surface Methodology

Mapping Intimacies ◽

10.21203/rs.3.rs-226555/v1 ◽

2021 ◽

Author(s):

P.SARAVANAN PANCHAMOORTHY ◽

R.Rajesh Kannan

Keyword(s):

Trichoderma Reesei ◽

Cellulase Production ◽

Medium Composition ◽

Central Component ◽

Pomegranate Peel ◽

Component Design ◽

Media Component ◽

Soybean Cake ◽

The Media ◽

Burman Design

Abstract In this work, pomegranate peels (PPs) otherwise considered as waste is used as substrates for producing cellulase. Pomegranate peel was employed as a carbon source to be produced of cellulase in submerged state fermentation (SmF). Statistical experimental designs were employed to screen the nutrients and optimize the media composition for cellulase production by Trichoderma reesei. Nine nutrients were selected by the preliminary screening of medium component production by plackett –burman design (PBD) technique. Four nutrients have been found to be important for cellulase production and optimized by central component design (CCD). The best media component for submerged fermentation of cellulase using pomegranate peel were avicel– 24.812 g/l, KH2PO4 – 4.626 g/l, soybean cake flour – 20.7 g/l and MnSO4.H2O-1.036 g/l. Under these optimum conditions, the production of cellulase was found to be 9.3 IU/mL. The optimum factors acquired from the statistical model were further confirmed using the experimental results.

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Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

Enzyme Research ◽

10.1155/2012/157643 ◽

2012 ◽

Vol 2012 ◽

pp. 1-7 ◽

Cited By ~ 10

Author(s):

P. Saravanan ◽

R. Muthuvelayudham ◽

T. Viruthagiri

Keyword(s):

Trichoderma Reesei ◽

Cellulase Activity ◽

Statistical Design ◽

Cellulase Production ◽

Positive Influence ◽

Mango Peel ◽

Soybean Cake ◽

Burman Design ◽

Plackett Burman Design ◽

Cake Flour

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.

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Cellulase production and morphology of Trichoderma reesei in different experimental conditions

Studia Universitatis Babeş-Bolyai Biologia ◽

10.24193/subbbiol.2018.2.09 ◽

2018 ◽

Vol 63 (2) ◽

pp. 115-129

Author(s):

Rahela Carpa ◽

◽

Alin Cândea ◽

Alexei Remizovschi ◽

Lucian Barbu-Tudoran ◽

...

Keyword(s):

Trichoderma Reesei ◽

Cellulase Production ◽

Experimental Conditions

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Growth of Dracaena marginata and Spathiphyllum ‘Petite’ in Sphagnum Peat- and Coconut Coir Dust-based Growing Media

Journal of Environmental Horticulture ◽

10.24266/0738-2898-17.1.49 ◽

1999 ◽

Vol 17 (1) ◽

pp. 49-52 ◽

Cited By ~ 4

Author(s):

Robert H. Stamps ◽

Michael R. Evans

Keyword(s):

Growth Parameters ◽

Cocos Nucifera ◽

Greenhouse Production ◽

Growing Media ◽

Sphagnum Peat ◽

Affected Plant ◽

Coir Dust ◽

Media Component ◽

The Media ◽

Cocos Nucifera L

Abstract A comparison was made of Canadian sphagnum peat (SP) and Philippine coconut (Cocos nucifera L.) coir dust (CD) as growing media components for greenhouse production of Dracaena marginata Bak. and Spathiphyllum Schott ‘Petite’. Three soilless foliage plant growing mixes (Cornell, Hybrid, University of Florida #2 [UF-2]) were prepared using either SP or CD and pine bark (PB), vermiculite (V), and/or perlite (P) in the following ratios (% by vol): Cornell = 50 CD or SP:25 V:25 P, Hybrid = 40 CD or SP:30 V:30 PB, UF-2 = 50 CD or SP: 50 PB. Dracaena root growth was not affected by treatments but there were significant mix × media component interactions that affected plant top growth parameters. In general, the growth and quality of D. marginata were reduced by using CD in Cornell, had no effect in Hybrid, and increased in UF-2. S. ‘Petite’ grew equally well in all growing mixes regardless of whether CD or SP was used; however, plants grew more in Cornell and Hybrid than in UF-2. S. ‘Petite’ roots, which were infested with Cylindrocladium spathiphylli, had higher grades when grown in CD than when the media contained SP.

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Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

BMC Biotechnology ◽

10.1186/s12896-021-00681-y ◽

2021 ◽

Vol 21 (1) ◽

Author(s):

Ján Krahulec ◽

Martin Šafránek

Keyword(s):

Recombinant Proteins ◽

Fermentation Process ◽

High Impact ◽

Production Level ◽

Media Component ◽

Component Supplier ◽

The Media ◽

The Difference ◽

Optimisation Procedure ◽

The Ideal

Abstract Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed the optimisation process for the production of enterokinase by improving the fermentation process. Enterokinase is the ideal enzyme for removing fusion partners from target recombinant proteins. In this study, we focused our optimization efforts on the sources of cultivation media components. YPD media components were chosen as variables for these experiments. Several suppliers for particular components were combined and the optimisation procedure was performed in 24-well plates. Peptone had the highest impact on enterokinase production, where the difference between the best and worst results was threefold. The least effect on the production level was recorded for yeast extract with a 1.5 fold difference. The worst combination of media components had a activity of only 0.15 U/ml and the best combination had the activity of 0.88 U/ml, i.e., a 5.87 fold difference. A substantially higher impact on the production level of enterokinase was observed during fermentation in two selected media combinations, where the difference was almost 21-fold. Conclusions Results demonstrated in the present study show that the media components from different suppliers have high impact on enterokinase productivity and also provide the hypothesis that the optimization process should be multidimensional and for achieving best results it is important to perform massive process also in terms of the particular media component supplier .

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cAMP activates calcium signalling via phospholipase C to regulate cellulase production in the filamentous fungus Trichoderma reesei

Biotechnology for Biofuels ◽

10.1186/s13068-021-01914-0 ◽

2021 ◽

Vol 14 (1) ◽

Author(s):

Yumeng Chen ◽

Xingjia Fan ◽

Xinqing Zhao ◽

Yaling Shen ◽

Xiangyang Xu ◽

...

Keyword(s):

Signal Transduction ◽

Phospholipase C ◽

Trichoderma Reesei ◽

Filamentous Fungus ◽

Calcium Signalling ◽

Signal Transduction Pathway ◽

Cellulase Production ◽

Signalling Pathway ◽

Dependent Manner ◽

Transduction Pathway

Abstract Background The filamentous fungus Trichoderma reesei is one of the best producers of cellulase and has been widely studied for the production of cellulosic ethanol and bio-based products. We previously reported that Mn2+ and N,N-dimethylformamide (DMF) can stimulate cellulase overexpression via Ca2+ bursts and calcium signalling in T. reesei under cellulase-inducing conditions. To further understand the regulatory networks involved in cellulase overexpression in T. reesei, we characterised the Mn2+/DMF-induced calcium signalling pathway involved in the stimulation of cellulase overexpression. Results We found that Mn2+/DMF stimulation significantly increased the intracellular levels of cAMP in an adenylate cyclase (ACY1)-dependent manner. Deletion of acy1 confirmed that cAMP is crucial for the Mn2+/DMF-stimulated cellulase overexpression in T. reesei. We further revealed that cAMP elevation induces a cytosolic Ca2+ burst, thereby initiating the Ca2+ signal transduction pathway in T. reesei, and that cAMP signalling causes the Ca2+ signalling pathway to regulate cellulase production in T. reesei. Furthermore, using a phospholipase C encoding gene plc-e deletion strain, we showed that the plc-e gene is vital for cellulase overexpression in response to stimulation by both Mn2+ and DMF, and that cAMP induces a Ca2+ burst through PLC-E. Conclusions The findings of this study reveal the presence of a signal transduction pathway in which Mn2+/DMF stimulation produces cAMP. Increase in the levels of cAMP activates the calcium signalling pathway via phospholipase C to regulate cellulase overexpression under cellulase-inducing conditions. These findings provide insights into the molecular mechanism of the cAMP–PLC–calcium signalling pathway underlying cellulase expression in T. reesei and highlight the potential applications of signal transduction in the regulation of gene expression in fungi.

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