scholarly journals Impact of media components from different suppliers on enterokinase productivity in Pichia pastoris

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Ján Krahulec ◽  
Martin Šafránek
Keyword(s):  
Recombinant Proteins ◽  
Fermentation Process ◽  
High Impact ◽  
Production Level ◽  
Media Component ◽  
Component Supplier ◽  
The Media ◽  
The Difference ◽  
Optimisation Procedure ◽  
The Ideal

Abstract Background The aim of this study was to provide an information about the homogeneity on the level of enterokinase productivity in P. pastoris depending on different suppliers of the media components. Results In previous studies, we performed the optimisation process for the production of enterokinase by improving the fermentation process. Enterokinase is the ideal enzyme for removing fusion partners from target recombinant proteins. In this study, we focused our optimization efforts on the sources of cultivation media components. YPD media components were chosen as variables for these experiments. Several suppliers for particular components were combined and the optimisation procedure was performed in 24-well plates. Peptone had the highest impact on enterokinase production, where the difference between the best and worst results was threefold. The least effect on the production level was recorded for yeast extract with a 1.5 fold difference. The worst combination of media components had a activity of only 0.15 U/ml and the best combination had the activity of 0.88 U/ml, i.e., a 5.87 fold difference. A substantially higher impact on the production level of enterokinase was observed during fermentation in two selected media combinations, where the difference was almost 21-fold. Conclusions Results demonstrated in the present study show that the media components from different suppliers have high impact on enterokinase productivity and also provide the hypothesis that the optimization process should be multidimensional and for achieving best results it is important to perform massive process also in terms of the particular media component supplier .

scholarly journals The intimacy effect: Girls’ reflections about pornography and ‘actual sex’

Sexualities ◽  
2020 ◽  
Vol 23 (7) ◽  
pp. 1248-1263
Author(s):  
Sanna Spišák
Keyword(s):  
Everyday Life ◽  
Social Relations ◽  
Online Survey ◽  
Media Education ◽  
Cultural Norms ◽  
Awareness Campaign ◽  
The Media ◽  
Youth Club ◽  
The Difference ◽  
The Ideal

Drawing on an online survey that was conducted as part of the media education awareness campaign of a Finnish online youth club, this article explores girls’ conceptions of the difference between pornographic representations and ‘actual sex’. As a result of the analysis, the greatest detachment between these two occurs concerning intimacy. Thus, this article will explore the pervasive influence of the notion of romantic intimacies, which provides the ideal for ‘normal’ relationships, sexual encounters and ‘good sex’ for the girls who participated in the survey through the concept of ‘the intimacy effect’. Like narratives of sexual storytelling in general, the survey dataset helps to trace connections between the personal and the societal. The dataset also draws attention to the ties between social relations and the cultural forms that mediate how these relations are set in motion, and to how certain cultural norms are forcefully influencing girls’ everyday life.

Growth of Dracaena marginata and Spathiphyllum ‘Petite’ in Sphagnum Peat- and Coconut Coir Dust-based Growing Media

1999 ◽  
Vol 17 (1) ◽  
pp. 49-52 ◽  
Author(s):  
Robert H. Stamps ◽  
Michael R. Evans
Keyword(s):  
Growth Parameters ◽  
Cocos Nucifera ◽  
Greenhouse Production ◽  
Growing Media ◽  
Sphagnum Peat ◽  
Affected Plant ◽  
Coir Dust ◽  
Media Component ◽  
The Media ◽  
Cocos Nucifera L

Abstract A comparison was made of Canadian sphagnum peat (SP) and Philippine coconut (Cocos nucifera L.) coir dust (CD) as growing media components for greenhouse production of Dracaena marginata Bak. and Spathiphyllum Schott ‘Petite’. Three soilless foliage plant growing mixes (Cornell, Hybrid, University of Florida #2 [UF-2]) were prepared using either SP or CD and pine bark (PB), vermiculite (V), and/or perlite (P) in the following ratios (% by vol): Cornell = 50 CD or SP:25 V:25 P, Hybrid = 40 CD or SP:30 V:30 PB, UF-2 = 50 CD or SP: 50 PB. Dracaena root growth was not affected by treatments but there were significant mix × media component interactions that affected plant top growth parameters. In general, the growth and quality of D. marginata were reduced by using CD in Cornell, had no effect in Hybrid, and increased in UF-2. S. ‘Petite’ grew equally well in all growing mixes regardless of whether CD or SP was used; however, plants grew more in Cornell and Hybrid than in UF-2. S. ‘Petite’ roots, which were infested with Cylindrocladium spathiphylli, had higher grades when grown in CD than when the media contained SP.

SEMINAL BREAKTHROUGH IN TACTILE PICTOGRAM DESIGN FOR VISUALLY IMPAIRED IN TRAIN STATION

2020 ◽  
Vol 13 (2) ◽  
pp. 55-60
Author(s):  
Fariz Fadhlillah
Keyword(s):  
Public Transportation ◽  
Visually Impaired ◽  
Great Opportunity ◽  
Train Station ◽  
Impaired Ability ◽  
Semiotic Approach ◽  
The Media ◽  
Impaired Person ◽  
Pictorial Form ◽  
The Ideal

One of the ideal public transportation facilities for the visually impaired in daily activities is trains. To be used at maximum, there is a need for communicative media to support the independence of orientation and mobility for the visually impaired in the train station. The media plays a role in supporting visually impaired individuals to know where they are, where to go, and how to reach the destination. The previous result regarding visually impaired ability to identify pictorial form which is designed with Primadi Tabrani’s ancient visual language semiotic approach shows a great opportunity for a pictogram to be the solution. However, the challenge is how to make the visually impaired person understand the meaning description that has been designed into tactile pictogram by touch. Basic consideration in designing process is the clarity of visual form when being touched, which is influenced by the way the shape is drawn and the tactile height

Madrasas and the Making of Islamic Womanhood

2018 ◽  
Author(s):  
Hem Borker
Keyword(s):  
Religious Institutions ◽  
Marriage Education ◽  
Women's Participation ◽  
Women's Lives ◽  
The Media ◽  
Education And Employment ◽  
A Site ◽  
Anthropological Literature ◽  
Educational Journeys ◽  
The Ideal

This ethnography provides a theoretically informed account of the educational journeys of students in girls’ madrasas in India. It focuses on the unfolding of young women’s lives as they journey from home to madrasa and beyond. Using a series of ethnographic portraits and bringing together the analytical concepts of community, piety, and aspiration, it highlights the fluidity of the essences of the ideal pious Muslim woman. It illustrates how the madrasa becomes a site where the ideals of Islamic womanhood are negotiated in everyday life. At one level, girls value and adopt practices taught in the madrasa as essential to the practice of piety (amal). At another level, there is a more tactical aspect to cultivating one’s identity as a madrasa-educated Muslim girl. The girls invoke the virtues of safety, modesty, and piety learnt in the madrasa to reconfigure conventional social expectations around marriage, education, and employment. This becomes more apparent in the choices exercised by the girls after leaving the madrasa, highlighted in this book through narratives of madrasa alumni pursuing higher education at a central university in Delhi. The focus on journeys of girls over a period of time, in different contexts, complicates the idealized and coherent notions of piety presented by anthropological literature on women’s participation in Islamic piety projects. Further, the educational stories of girls challenge the media and public representations of madrasas in India, which tend to caricature them as outmoded religious institutions with little relevance to the educational needs of modernizing India. Mapping madrasa students’ personal journeys of becoming educated while leading pious lives allows us to see how these young women are reconfiguring notions of Islamic womanhood.

scholarly journals A Secular Critique of Religious Ethics and Politics

2017 ◽  
Author(s):  
Paul Cliteur
Keyword(s):  
Extreme Case ◽  
Moral Autonomy ◽  
Religious Ethics ◽  
Religious Leader ◽  
Political Autonomy ◽  
Central Notion ◽  
The Difference ◽  
Do So ◽  
The Ideal ◽  
Ethics And Politics

This chapter discusses the difference between a nonsecular or religious critique of religious ethics and politics and a specifically secular critique. It introduces the central notion of a secular critique, autonomy, and its two types, moral and political. Moral autonomy entails the separation of religion from ethics. The ideal of making that separation is called “moral secularism.” The opposite of moral autonomy is “moral heteronomy.” An extreme case of moral heteronomy is discussed: Abraham’s willingness to sacrifice his own son when God commanded him to do so. Next, the importance of political autonomy and political secularism is illustrated with reference to the conflict between the king Ahab (the model of a secular ruler) and the prophet Elijah (the model of a religious leader). Some stories in the holy scriptures of the monotheist religions held in common by Judaism, Christianity, and Islam are unfavorable toward secularism (both moral and political).

Design of Measurement System of the Center of Gravity

2013 ◽  
Vol 706-708 ◽  
pp. 733-736
Author(s):  
Fang Li
Keyword(s):  
Measurement System ◽  
Center Of Gravity ◽  
Actual Position ◽  
Ideal Position ◽  
The Future ◽  
The Difference ◽  
New System ◽  
The Ideal

It is very difficult to confirm the position of the center of gravity irregular object.This paper introduces a new system,which can be used in measuring and adjusting of the center of gravity.The irregular object was put on the support parts,then the center of gravity was given.When the actual position was compared with the ideal position,the difference was given. Until actual position and ideal position were coincided,the machining was continuing. Matlab was programmed in this measure system. It will be applied widely in the future.

Producing Steam by Spraying Water on a Heated Bed of Steel Spheres

2021 ◽  
pp. 1-42
Author(s):  
D. Jordan Bouchard ◽  
HengSheng Yang ◽  
Sanjeev Chandra
Keyword(s):  
Particle Size ◽  
Thermal Mass ◽  
Steam Generation ◽  
Bed Porosity ◽  
The Media ◽  
Effect Of Particle Size ◽  
The Difference ◽  
Rate Of Evaporation ◽  
A Minor ◽  
Counter Current

Abstract Steam generators used in industrial baking ovens operate by pouring or spraying water on a preheated thermal mass. This paper presents a methodology to quantify the amount of steam generated from a thermal mass along with experiments to determine the effect of particle size and porosity on steam generation. Three sizes of steel spheres, 0.6 mm, 8 mm, and 16 mm in diameter, were used to construct porous media beds that were preheated in an oven after which water was sprayed onto them from a full-cone nozzle for a fixed duration. The weight of the heated bed and the impinging water were recorded during spraying. The difference in weight change when spraying on heated and unheated beds gave the rate of evaporation. Thermocouples were used to record the internal temperature of the bed. Steam generation rate increased with particle size while bed porosity had only a minor influence. The counter-current flow of steam within the media bed disrupts the downward flow of water enough to leave pockets of hot material, reducing steam production. To maximize steam generation the media size, material, and spray time should be matched to ensure the surfaces of particles remain above the boiling point of water during spraying.

Physical properties of biaxially oriented PA6 film for simultaneous stretching and sequential processing

2011 ◽  
Vol 31 (1) ◽  
Author(s):  
Masao Takashige ◽  
Toshitaka Kanai
Keyword(s):  
Impact Strength ◽  
Physical Properties ◽  
Physical Property ◽  
High Impact ◽  
Thermal Shrinkage ◽  
Double Bubble ◽  
Biaxial Stretching ◽  
High Impact Strength ◽  
The Difference ◽  
Stretching Process

Abstract There are two different stretching processes that produce the biaxially oriented film, namely the tenter process and double bubble tubular film process. Furthermore, there are two tenter processes, i.e., the sequential biaxial stretching process and simultaneous biaxial stretching process. There is no report describing the difference among film physical properties of the three different processes. The biaxially oriented polyamide film using the double bubble tubular process has good balanced physical property and high impact strength, thus it is used for proper applications utilizing their advantage properties. In this report, the influence of each biaxial stretching process on film physical properties of polyamide, which has hydrogen bond, was studied in detail. As a result, the tentering process film has anisotropic tensile properties between machine direction (MD) and transverse direction (TD). This result was influenced by a later stretching process, namely TD stretching. On the contrary, the double bubble tubular film has good balanced properties, especially thermal shrinkage and impact strength. Tentering simultaneous stretching film has much larger shrinkage in MD than in TD. The sequential stretching film has larger shrinkage in TD than in MD. The double bubble tubular film has high impact strength, because it corresponds to the balanced molecular orientation.

scholarly journals The Impact of Media in the Socialization Process in Albania

2014 ◽  
Vol 1 (1) ◽  
pp. 149
Author(s):  
Bukurie Lila
Keyword(s):  
Young Adults ◽  
Young People ◽  
Mass Media ◽  
Positive Function ◽  
Main Question ◽  
Negative Effects ◽  
Socialization Process ◽  
The Media ◽  
The Impact ◽  
The Ideal

Media is one of the main agents of socialization that affects youth the most. Young adults are majority time are surrounded by the media, which brings me to my main question, "How is Mass Media Affecting Socialization in Children and Young Adults in Albania?" To understand this question one must know and understand what socialization is. The socialization process is a very dramatic impact on a child's life. Socialization is a "Continuing process whereby an individual acquires a personal identity and learns the norms, values, behavior, and social skills appropriate to his or her social position". Mass media has enormous effects on our attitudes and behavior which makes it an important contributor to the socialization process. in some ways mass media can serve as a positive function. It helps there to be more diversity, we can learn more about things that are going on in different countries. It can help you learn new things you did not know. Sadly Media can serve as a negative function in young people life. Young people want to be accepted by society and the media creates the ideal image that tells you what the characteristics are to be accepted and to be able to fit in with society. They show what you should look like, how you can look like this, and where to go to buy these things that will make you look right. This is why many young women deal with anorexia because they want to look like the ideal type that the media displays. Media also influences young people to misbehave. Media shows that being deviant makes you cool and look tough and that it's okay to do deviant things. Statistics show that when young people watch violence on television it increases their appetites to become involved in violence. It opens their minds to violence and makes them aware of crimes and people acting deviant. Many people think that the media does not play a role in the socialization process as much as family, peers and education. But in fact the media plays a strong role in the socialization process. The aim of this study is to see the positive and negative effects that the Albanian media plays in the socialization process in Albania.

scholarly journals Optimization of an Economical Medium Composition For The Coculture of Clostridium Butyricum And Bacillus Coagulans

2021 ◽  
Author(s):  
Yonghong Li ◽  
Yun Wang ◽  
Yingying Liu ◽  
Xuan Li ◽  
Keke Li ◽  
...  
Keyword(s):  
Industrial Production ◽  
Fermentation Process ◽  
Bacillus Coagulans ◽  
Medium Composition ◽  
Clostridium Butyricum ◽  
Production Level ◽  
Beneficial Bacteria ◽  
Spore Yield ◽  
Viable Bacteria ◽  
Optimal Medium

Abstract Clostridium butyricum is a spore-forming probiotic existing in the intestines of humans and animals which can promote the enhancement of beneficial bacteria and maintain intestinal microecological balance. However, it is difficult to improve the production level of C. butyricum by conventional fermentation process. In this study, a co-fermentation process of C. butyricum DL-1 and Bacillus coagulans ZC2-1 was established to improve the viable counts and spore yield of C. butyricum, and the formula of coculture medium was optimized by flask fermentation. The results showed that the optimum medium composition is bran 10 g/L, corn steep powder 15 g/L, peptone 15 g/L, K2HPO4 1 g/L and MnSO4 0.5 g/L. Cultured stationarily in the optimal medium for 36 h, the number of viable bacteria of C. butyricum DL-1 reached 1.5×108 CFU/mL and the spore forming rate was 92.6%. The results revealed an economical and effective medium composition for the coculture of C. butyricum and B. coagulans. The co-fermentation process established in this study provides a new fermentation mode for the industrial production of other absolute anerobic bacteria.

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