scholarly journals Antibodies against dextransucrase from Streptococcus mutans display anti-biofilm and growth suppressing activities

2021 ◽  
Author(s):  
Shabeer Ahmad Rather ◽  
Akhtar Mahmood ◽  
Lakhvinder Singh ◽  
Alka Bhatia ◽  
Sukesh Chander Sharma ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Membrane Fraction ◽  
Inhibitory Effect ◽  
Cross Reactivity ◽  
Plaque Formation ◽  
Hydrophobic Properties ◽  
Experimental Conditions ◽  
Glass Surfaces ◽  
Reduced Adherence

Abstract Dextransucrase produced by Streptococcus mutans play an essential role in the formation of dental caries by synthesizing exopolysaccharides from sucrose, an important metabolite of the organism. In this study we report the location of dextransucrase in Streptococcus mutans cells and describe that antibodies raised against dextransucrase inhibited biofilm formation and reduced the adherence and hydrophobic properties of Streptococcus mutans. Western blot analysis and immunoelectron microscopy revealed that dextransucrase is located abundantly in the membrane fraction in S. mutans cells. Scanning electron microscopy and fluorescence microscopy revealed reduced cell density, impaired bioflim (plaque) formation in presence of dextransucrase antibodies. Genes associated with bioflim formation in S. mutans such as GtfB, GtfC, BrpA, relA, Smu630, vicK were down regulated (50–97%) in presence of the enzyme antibody. Presence of enzyme antibodies reduced adherence of S. mutans cells to glass surfaces by 58% and hydrophobicity by 55.2%. However dextransucrase antibodies did not affect acid production by S. mutans, under the experimental conditions. Immunohistochemistry studies with certain human samples displayed no cross reactivity with dextransucrase antibody. These findings suggest that antibodies against dextransucrase exhibit a profound inhibitory effect on the vital cariogenic factors of S. mutans and have no cross reactivity with human tissues tested, thus implying that dextransucrase could be a promising antigen to study its anticariogenic potential.

Anti-Biofilm Formation of Streptococcus mutans by Jasmine Mouthwash

2018 ◽  
Vol 773 ◽  
pp. 323-327
Author(s):  
Sroisiri Thaweboon ◽  
Boonyanit Thaweboon
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antimicrobial Activities ◽  
Inhibitory Effect ◽  
Negative Control ◽  
Oral Streptococci ◽  
Drug Of Choice ◽  
Causative Microorganism ◽  
Warm Temperate

Streptococcus mutans has been reported to be a major causative microorganism for oral biofilm associated with dental caries. Jasmine sambac or Arabian jasmine is a species of jasmine native to tropical and warm temperate regions particularly West and Southeast Asia. The antimicrobial activities of essential oil extracted from the flowers of J. sambac have been shown to attract researchers. Objective: To determine the anti-biofilm formation of S. mutans by mouthwash containing jasmine oil. Materials and Methods: S. mutans KPSK2, the cariogenic strain of oral streptococci was used in the study. The 24-h biofilms of S. mutans were formed on polystyrene plates treated with jasmine mouthwash. The 0.2% chlorhexidine gluconate and phosphate buffer saline mouthwash were used as a positive and negative control respectively. The amount of biofilm was quantified by crystal violet staining and spectrophotometry at an optical density of 595 nm. Results: Jasmine mouthwash showed a significant inhibitory effect on S. mutans biofilm formation by decreasing 43% of biofilm whereas that of chlorhexidine showed 71% reduction. Conclusion: The anti-biofilm formation property of jasmine mouthwash was elucidated; therefore it might be another drug of choice that can be used as an adjunct to control the oral health in the prevention of dental caries.

scholarly journals Antibiofilm Activity of Chilean Propolis onStreptococcus mutansIs Influenced by the Year of Collection

2015 ◽  
Vol 2015 ◽  
pp. 1-6 ◽  
Author(s):  
Jorge Jesús Veloz ◽  
Nicolás Saavedra ◽  
Alexis Lillo ◽  
Marysol Alvear ◽  
Leticia Barrientos ◽  
...  
Keyword(s):  
Chemical Composition ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Antibacterial Effect ◽  
Inhibitory Effect ◽  
Biological Properties ◽  
Antibiofilm Activity ◽  
Total Polyphenols

The chemical composition of propolis varies according to factors that could have an influence on its biological properties. Polyphenols from propolis have demonstrated an inhibitory effect onStreptococcus mutansgrowth. However, it is not known if different years of propolis collection may affect its activity. We aimed to elucidate if the year of collection of propolis influences its activity onStreptococcus mutans. Polyphenol-rich extracts were prepared from propolis collected in three different years, characterized by LC-MS and quantified the content of total polyphenols and flavonoids groups. Finally, was evaluated the antibacterial effect onStreptococcus mutansand the biofilm formation. Qualitative differences were observed in total polyphenols, flavones, and flavonols and the chemical composition between the extracts, affecting the strength of inhibition of biofilm formation but not the antimicrobial assays. In conclusion, chemical composition of propolis depends on the year of collection and influences the strength of the inhibition of biofilm formation.

scholarly journals Inhibitory Effect ofBacillus velezensison Biofilm Formation byStreptococcus mutans

2018 ◽  
Author(s):  
Yesol Yoo ◽  
Dong-Ho Seo ◽  
Hyunjin Lee ◽  
Young-Do Nam ◽  
Myung-Ji Seo
Keyword(s):  
Dental Caries ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Culture Medium ◽  
Focal Point ◽  
Cell Surface Hydrophobicity ◽  
Surface Hydrophobicity ◽  
Inhibitory Effect ◽  
Fermented Foods ◽  
Bacillus Velezensis

ABSTRACTStreptococcus mutansplays a key role in the development of dental caries and promotes the formation of oral biofilm produced by glucosyltransferases (GTFs).Bacillus velezensisK68 was isolated from traditional fermented foods and inhibits biofilm formation mediated byS. mutans. Gene amplification results demonstrated thatB. velezensisK68 contained genes for the biosynthesis of 1-deoxynojirimycin (1-DNJ), a known GTF expression inhibitor. The presence of the GabT1, Yktc1, and GutB1 genes required for 1-DNJ synthesis inB. velezensisK68 was confirmed. Supernatant fromB. velezensisK68 culture medium inhibited biofilm formation by 84% whenS. mutanswas cultured for 48 h, and inhibited it maximally when 1% glucose was added to theS. mutansculture medium as a GTF substrate. In addition, supernatant fromB. velezensisK68 medium containing 3 ppb 1- DNJ decreasedS. mutanscell surface hydrophobicity by 79.0 ± 0.8% compared with that of untreated control. The supernatant containing 1-DNJ decreasedS. mutansadherence by 99.97% and 98.83% under sugar-dependent and sugar-independent conditions, respectively.S. mutanstreated with the supernatant exhibited significantly reduced expression of the essential GTF genesgtfB,gtfC,andgtfDcompared to that in the untreated group. Thus,B. velezensisinhibits the biofilm formation, adhesion, and GTF gene expression ofS. mutansthrough 1- DNJ production.IMPORTANCEDental caries is among the most common infectious diseases worldwide, and its development is closely associated with physiological factors of bacteria, such as the biofilm formation and glucosyltransferase production ofStreptococcus mutans.Biofilms are difficult to remove once they have formed due to the exopolysaccharide matrix produced by the microorganisms residing in them; thus, inhibiting biofilm formation is a current focal point of research into prevention of dental caries. This study describes the inhibitory properties ofBacillus velezensisK68, an organism isolated from traditional Korean fermented foods, against biofilm formation byS. mutans. Herein, we show thatB. velezensisinhibits the biofilm formation, adherence to surfaces, and glucosyltransferase production ofS. mutans.

scholarly journals Effects of resveratrol on cariogenic virulence properties of Streptococcus mutans

2020 ◽  
Author(s):  
Jinheng Li ◽  
Tiantian Wu ◽  
Weiwei Peng ◽  
yaqin zhu
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Acid Production ◽  
Virulence Gene ◽  
Acid Tolerance ◽  
Extracellular Polysaccharide ◽  
Inhibitory Effect ◽  
Water Soluble ◽  
Virulence Gene Expression ◽  
Virulence Properties

Abstract Background: Streptococcus mutans is the principal etiological agent of human dental caries. The major virulence factors of S. mutans are acid production, acid tolerance, extracellular polysaccharide (EPS) synthesis and biofilm formation. The aim of this study is to evaluate the effect of resveratrol, a natural compound, on virulence properties of S. mutans . Results: Resveratrol at sub-MIC levels significantly decreased acid production and acid tolerance, inhibited synthesis of water-soluble polysaccharide and water-insoluble polysaccharide, compromised biofilm formation. Related virulence gene expression ( ldh, relA, gtfC, comDE ) was down-regulated with increasing concentrations of resveratrol. Conclusions : Resveratrol has an inhibitory effect on S. mutans cariogenic virulence properties and it represents a promising anticariogenic agent. Keywords : resveratrol, Streptococcus mutans , acidogenicity, aciduricity, extracellular polysaccharide, biofilm

scholarly journals Polyphenols Inhibit Candida albicans and Streptococcus mutans Biofilm Formation

2019 ◽  
Vol 7 (2) ◽  
pp. 42 ◽  
Author(s):  
Yosi Farkash ◽  
Mark Feldman ◽  
Isaac Ginsburg ◽  
Doron Steinberg ◽  
Miriam Shalish
Keyword(s):  
Candida Albicans ◽  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Confocal Scanning Laser Microscopy ◽  
Total Biomass ◽  
Biofilm Inhibition ◽  
Confocal Scanning ◽  
Confocal Scanning Laser ◽  
Biofilm Development

Background: Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) are two major contributors to dental caries. They have a symbiotic relationship, allowing them to create an enhanced biofilm. Our goal was to examine whether two natural polyphenols (Padma hepaten (PH) and a polyphenol extraction from green tea (PPFGT)) could inhibit the caries-inducing properties of S. mutans and C. albicans. Methods: Co-species biofilms of S. mutans and C. albicans were grown in the presence of PH and PPFGT. Biofilm formation was tested spectrophotometrically. Exopolysaccharides (EPS) secretion was quantified using confocal scanning laser microscopy. Biofilm development was also tested on orthodontic surfaces (Essix) to assess biofilm inhibition ability on such an orthodontic appliance. Results: PPFGT and PH dose-dependently inhibited biofilm formation without affecting the planktonic growth. We found a significant reduction in biofilm total biomass using 0.625 mg/mL PPFGT and 0.16 mg/mL PH. A concentration of 0.31 mg/mL PPFGT and 0.16 mg/mL PH inhibited the total cell growth by 54% and EPS secretion by 81%. A reduction in biofilm formation and EPS secretion was also observed on orthodontic PVC surfaces. Conclusions: The polyphenolic extractions PPFGT and PH have an inhibitory effect on S. mutans and C. albicans biofilm formation and EPS secretion.

scholarly journals EFFECT OF CURCUMA LONGA EXTRACT ON BIOFILM FORMATION BY STREPTOCOCCUS MUTANS

2017 ◽  
Vol 10 (7) ◽  
pp. 186
Author(s):  
Gokul G ◽  
Geetha Rv
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Inhibitory Action ◽  
Curcuma Longa ◽  
Saliva Sample ◽  
Inhibitory Effect ◽  
Tryptone Soya Agar

Objective: To find the effect of Curcuma longa extract on biofilm formation by Streptococcus mutans.Methods: The organism S. mutans was isolated from saliva sample using special media (Mutans - sanguis agar) and maintained in tryptone soya agar at 4°C in Department of Microbiology, Saveetha Dental College and Hospitals.Results: From the study, we infer that 78.35% of inhibition of the biofilm formation is seen with 100 ug of curcumin extract. From the result, it is evident that curcumin has a very good inhibitory effect on S. mutans growth.Conclusion: The study concludes the inhibitory action of curcumin on S. mutans by preventing biofilm formation.

scholarly journals Investigation on the effect of vitamin C on growth & biofilm-forming potential of Streptococcus mutans isolated from patients with dental caries

2020 ◽  
Author(s):  
Zehdi Eydou ◽  
Bader Naser Jad ◽  
Zeyad Elsayed ◽  
Anas Ismail ◽  
Michael Magaogao ◽  
...  
Keyword(s):  
Dental Caries ◽  
Vitamin C ◽  
Streptococcus Mutans ◽  
Crystal Violet ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Clinical Isolates ◽  
Zone Of Inhibition ◽  
Negative Effect ◽  
Biofilm Prevention

Abstract Background: Streptococcus mutans is a major cause of dental caries. Its capacity to produce biofilm is fundamental in the pathogenesis of this ubiquitous condition. As maintaining a healthy dentition is a genuine goal given the contemporary advance in caries control, researchers are striving to achieve a breakthrough in caries therapy. We are taking the anti-cariogenic properties of vitamin C a step-further, considering the well-known evidence of the inversely proportionate relationship between salivary levels of vitamin C and dental caries. The aim of this study was to determine MIC, MBC, biofilm prevention concentration (BPC), and derivative measures of vitamin C against fresh clinical isolates of S. mutans to evaluate its efficacy as an anti-cariogenic agent.Results: Based on the data of four independent experiments done in quadruplicates, we found a concentration-dependent inhibitory effect of vitamin C on all S. mutans strains tested. The average MBC, MIC, and BPC of vitamin C were found to be 10.16, 9.38, and 5.61 mg/ml, respectively. Spectrophotometric quantitation of crystal violet showed diminished biofilm formation in the presence of vitamin C (p < 0.05). When compared with gentamicin, vitamin C produced a zone of inhibition that was three times as large against the clinical isolates.Conclusion: Our results show that vitamin C has a negative effect on S. mutans growth and biofilm formation. Being the first to meticulously utilize BPC to explore a well-known effect of vitamin C, this report aims to help in the instigation of trials of higher evidence that will ultimately culminate in repurposing vitamin C as a novel anti-cariogenic agent, albeit further studies are required to provide auxiliary evidence in this context.

scholarly journals Inhibitory effect of Bacillus velezensis on biofilm formation by Streptococcus mutans

2019 ◽  
Vol 298 ◽  
pp. 57-63 ◽  
Author(s):  
Yesol Yoo ◽  
Dong-Ho Seo ◽  
Hyunjin Lee ◽  
Eui-Sang Cho ◽  
Nho-Eul Song ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Bacillus Velezensis

scholarly journals Enterococcus faeciumWB2000 Inhibits Biofilm Formation by Oral Cariogenic Streptococci

2011 ◽  
Vol 2011 ◽  
pp. 1-5 ◽  
Author(s):  
Nao Suzuki ◽  
Masahiro Yoneda ◽  
Yuko Hatano ◽  
Tomoyuki Iwamoto ◽  
Yosuke Masuo ◽  
...  
Keyword(s):  
Streptococcus Mutans ◽  
Enterococcus Faecalis ◽  
Enterococcus Faecium ◽  
Biofilm Formation ◽  
Inhibitory Effect ◽  
Mutans Streptococci ◽  
Streptococcus Sobrinus ◽  
Dual Cultures

This study investigated the inhibitory effect of probioticEnterococcus faeciumWB2000 on biofilm formation by cariogenic streptococci. The ability ofE. faeciumWB2000 and JCM5804 andEnterococcus faecalisJCM5803 to inhibit biofilm formation by seven laboratory oral streptococcal strains and 13 clinical mutans streptococcal strains was assayed. TheEnterococcalstrains inhibited biofilm formation in dual cultures with the mutans streptococcal strainsStreptococcus mutansXc andStreptococcus sobrinusJCM5176 (P<0.05), but not with the noncariogenic streptococcal strains.Enterococcus faeciumWB2000 inhibited biofilm formation by 90.0% (9/10) of the clinicalS. mutansstrains and 100% (3/3) of the clinicalS. sobrinusstrains. After culturing, the pH did not differ between single and dual cultures. The viable counts of floating mutans streptococci were lower in dual cultures withE. faeciumWB2000 than in single cultures.Enterococcus faeciumWB2000 acted as a probiotic bacterial inhibitor of cariogenic streptococcal biofilm formation.

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