scholarly journals Effect of Hormones (IBA & IAA) on the Propagation of Himalayan Yew in Pakistan: A Conservation Approach

2020 ◽  
Author(s):  
Javaid Iqbal ◽  
Bushra Khan ◽  
Sardar Khan ◽  
Nasreen Ghaffar ◽  
Ishaq Ahmad Mian ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Medicinal Plants ◽  
Butyric Acid ◽  
Indole Acetic Acid ◽  
Average Length ◽  
Stem Cuttings ◽  
Indole Butyric Acid ◽  
Number Of Leaves ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Abstract Background Himalayan yew (Taxus wallichiana) is one of the endangered medicinal plants species having great importance due to the presence of anticancer drug Taxol. This metabolite is mainly used for the treatment of ovarian, breast, AIDS-related cancers, and other indications. The study being reported here was conducted for the propagation of Himalayan yew by using two different trials of Indole Butyric acid (IBA) and Indole acetic acid (IAA) hormones treatments (2000–7000 ppm) through stem cuttings (140 each). In the same way, 3840 cuttings were treated with IBA and IAA from November 2016 to November 2017 at Lalku valley, Swat, Khyber Pakhtunkhwa (KP), Pakistan. Results The influence of IBA treatment (7000 ppm) showed a survival of 85.22% (average number of roots = 10.4, average length of roots = 15.5 cm, average number of leaves = 92.4 and average number of sprouts = 3.3) while that of IAA treatment (7000 ppm) the survival of 81.11% (average number of roots = 9.1, average length of roots = 14.6 cm, average number of leaves = 84.0 and average number of sprout = 3.0) were more significant followed by 2000–6000 ppm (IBA and IAA). Lowest survival 40–45% (average number of roots = 4.2, average length of roots = 8.0 cm, average number of leaves = 32.2, average number of sprouts = 1.7) was noticed for controlled cuttings. Conclusion The present study enhanced the potential of conservation and propagation of T. wallichiana. Hence our study suggests and recommends the application of IBA (7000 ppm) as a better hormone for the conservation and propagation of Himalayan yew.

scholarly journals In vitro Plantlets Regeneration from Nodal Segments of Murici (Byrsonima gardneriana)

2018 ◽  
Vol 10 (4) ◽  
pp. 402
Author(s):  
Francisca S. Sá ◽  
Jorge M. P. Porto ◽  
Alone L. Brito ◽  
José R. F. Santana ◽  
Rafaeli A. V. Souza ◽  
...  
Keyword(s):  
Acetic Acid ◽  
Butyric Acid ◽  
Activated Charcoal ◽  
Indole Acetic Acid ◽  
Nodal Segments ◽  
Naphthaleneacetic Acid ◽  
Indole Butyric Acid ◽  
In Vitro Plantlets ◽  
In Vitro Micropropagation

This study aimed to develop efficient protocols for the in vitro micropropagation of Byrsonima gardneriana. Nodal segments were obtained from seedlings germinated in vitro with 60 days of life. These were inoculated in MS/2 supplemented with 87.64 µM of sucrose and solidified with 0.7% of agar, supplemented with different concentrations of cytokinin 6-benzylaminopurine (0.0; 2.0; 4.0 and 8.0 µM) associated with different concentrations of auxin, indole acetic acid (0.0; 0.5 and 1.0 µM) and naphthaleneacetic acid (0.0; 0.5 and 1.0 µM). The sprouting were individualized and transferred to MS/2 cultures with different concentrations of indole butyric acid (0.0; 1.0; 2.0 and 3.0 µM), and presence and absence of activated charcoal (1.0 g L-1). The use of concentrations from 2.0 to 4.0 µM 6-benzylaminopurine was efficient in the multiplication of B. gardneriana, given that, using concentrations above these, a decrease in this efficiency occurs. The use of auxin interfered negatively with the results. In vitro rooting occurs even in medium free of auxin. The activated charcoal was insufficient for rooting. The use of growth regulators 6-benzylaminopurine and indole butyric acid are efficient in micropropagation of B. gardneriana, however, further studies should be performed to optimize this protocol.

scholarly journals Adventitious root formation in branch cuttings of Taxus wallichiana Zucc.(Himalayan yew): A clonal approach to conserve the scarce resource

2017 ◽  
Vol 8 ◽  
Author(s):  
Mohd Aslam ◽  
Parvaiz A. Raina ◽  
Rouf Ur Rafiq ◽  
T.O. Siddiqi ◽  
Zafar A. Reshi
Keyword(s):  
Acetic Acid ◽  
Adventitious Root ◽  
Root Formation ◽  
Block Design ◽  
Natural Habitat ◽  
Adventitious Root Formation ◽  
Naphthalene Acetic Acid ◽  
Indole Butyric Acid ◽  
Himalayan Yew ◽  
Taxus Wallichiana

Himalayan yew (<em>Taxus wallichiana</em> Zucc.), is an economically valuable plant and critically endangered due to overexploitation for the isolation of Taxol,an exciting anticancer drug from its bark and leaves. Since the species is unisexual and due to its long seed dormancy period and rapid loss of viability coupled with low survival percentage, its natural regeneration from seeds is very poor. As the seed raised plants add little growth, propagation by stem cuttings was tried under natural conditions and a considerable success was achieved after making use of different auxins (Indole-3- Acetic Acid-IAA, Indole Butyric Acid-IBA and Naphthalene Acetic Acid-NAA) in different concentrations. A Randomized block design (RBD) was adopted for laying the experiment of the present study. Of the ten treatments studied, IBA at 500ppm performed best of all the treatments and registered higher callusing percentage, rooting percentage, number of roots and length of roots in the juvenile shoot cuttings of the species. The results achieved through the application ofplant growth regulators (PGRs)by way of adventitious root formation (ARF) could be useful for the management of this understory coniferous tree species, whether for conservation, habitat restoration or for the production of Taxol,a promising anti-cancer agent. The technique evolved will be the most handy, quickest, inexpensive and can be applied any where in its natural habitat for the restoration and restocking of this valuable plant, which is otherwise facing the peril of extinction throughout the range of its distribution including Indian Himalayas.

scholarly journals Pengendalian Kejadian Gugur Bunga dan Buah (Fruit-drop) dengan Aplikasi Indole Acetic Acid (IAA), Indole Butyric Acid (IBA) dan Giberelin Pada Tanaman Cabai (Capsicum annuum L.)

2020 ◽  
Vol 4 (1) ◽  
pp. 35-40
Author(s):  
Rahmansyah Dermawan ◽  
Ifayanti Ridwan Saleh ◽  
Katriani Mantja ◽  
Hari Iswoyo ◽  
St Salmiati
Keyword(s):  
Acetic Acid ◽  
Capsicum Annuum ◽  
Butyric Acid ◽  
Indole Acetic Acid ◽  
Indole Butyric Acid ◽  
Capsicum Annuum L ◽  
Fruit Drop

Penelitian bertujuan mendapatkan data dan informasi morfofisiologi kejadian gugur bunga dan buah (fruit-drop) pada tanaman cabai terhadap pemberian zat pengatur tumbuh (ZPT) auksin (IAA dan IBA) dan GA3. Penelitian disusun berdasarkan Rancangan Petak Terpisah dalam rancangan acak kelompok dengan 3 ulangan. Petak utama adalah 3 jenis ZPT yaitu IAA, IBA, dan GA3. Anak petak yaitu konsentrasi perlakuan yang terdiri dari 5 taraf (ppm) yaitu 0, 25, 50, 75, dan 100 ppm. Data yang diperoleh dianalis menggunakan software STAR dan jika terdapat beda nyata diuji lanjut dengan Beda Nyata Terkecil (BNT) pada taraf 5%. Hasil penelitian menunjukkan bahwa perlakuan IAA, IBA, dan GA3 serta konsentrasi perlakuannya tidak berpengaruh nyata terhadap jumlah bunga tinggal maupun bunga gugur pada tanaman cabai besar. Namun, hasil penelitian menemukan bahwa perlakuan ZPT menghasilkan jumlah bunga tinggal yang lebih banyak dibandingkan bunga gugur. Pemberian IAA 25 ppm menghasilkan jumlah buah tinggal tertinggi dibandingkan konsentrasi IAA lainnya. Peningkatan konsentrasi GA3 cenderung meningkatkan jumlah buah tinggal dan tertinggi pada konsentrasi GA3 100 ppm. Interaksi antara jenis ZPT dan konsentrasi pemberiannya berpengaruh nyata terhadap bobot per buah. Perlakuan GA3 100 ppm menghasilkan bobot per buah tertinggi. Peningkatan konsentrasi auksin cenderung menurunkan bobot per buah. Pemberian IBA 100 ppm menurunkan panjang buah cabai besar.

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