scholarly journals A synthetic receptor platform enables rapid and portable monitoring of liver dysfunction via engineered bacteria.

2021 ◽  
Author(s):  
Hung-Ju Chang ◽  
Ana Zuniga ◽  
Ismael Conejero ◽  
Peter Voyvodic ◽  
Jerome Gracy ◽  
...  
Keyword(s):  
Liver Dysfunction ◽  
High Performance ◽  
Limit Of Detection ◽  
Portable Monitoring ◽  
Monitoring And Control ◽  
Modular Assembly ◽  
E Coli ◽  
Synthetic Receptor ◽  
And Control ◽  
Engineered Bacteria

Abstract Bacterial biosensors, or bactosensors, are promising field-deployable agents for medical and environmental diagnostics. However, the lack of scalable frameworks to systematically program ligand detection limits their applications. Here we present a synthetic receptor platform, termed EMeRALD (Engineered Modularized Receptors Activated via Ligand-induced Dimerization) which supports the modular assembly of sensing modules onto a high-performance, generic signaling scaffold controlling gene expression in E. coli. We applied EMeRALD to detect bile salts, a biomarker of liver dysfunction, by repurposing sensing modules from enteropathogenic Vibrio species. We improved the sensitivity and lowered the limit-of-detection of the sensing module by directed evolution. We then engineered a colorimetric bactosensor detecting pathological bile salt levels in serum from patients having undergone liver transplant, providing an output detectable by the naked-eye. The EMeRALD technology enables functional exploration of natural sensing modules and rapid engineering of synthetic receptors for diagnostics, environmental monitoring, and control of therapeutic microbes.

scholarly journals A synthetic receptor platform enables rapid and portable monitoring of liver dysfunction via engineered bacteria.

2021 ◽  
Author(s):  
Hung-Ju Chang ◽  
Ana Zuniga ◽  
Ismael Conejero ◽  
Peter L Voyvodic ◽  
Jerome Gracy ◽  
...  
Keyword(s):  
Liver Dysfunction ◽  
High Performance ◽  
Limit Of Detection ◽  
Portable Monitoring ◽  
Monitoring And Control ◽  
Modular Assembly ◽  
E Coli ◽  
Synthetic Receptor ◽  
And Control ◽  
Engineered Bacteria

Bacterial biosensors, or bactosensors, are promising field-deployable agents for medical and environmental diagnostics. However, the lack of scalable frameworks to systematically program ligand detection limits their applications. Here we present a synthetic receptor platform, termed EMeRALD (Engineered Modularized Receptors Activated via Ligand-induced Dimerization) which supports the modular assembly of sensing modules onto a high-performance, generic signaling scaffold controlling gene expression in E. coli. We applied EMeRALD to detect bile salts, a biomarker of liver dysfunction, by repurposing sensing modules from enteropathogenic Vibrio species. We improved the sensitivity and lowered the limit-of-detection of the sensing module by directed evolution. We then engineered a colorimetric bactosensor detecting pathological bile salt levels in serum from patients having undergone liver transplant, providing an output detectable by the naked-eye. The EMeRALD technology enables functional exploration of natural sensing modules and rapid engineering of synthetic receptors for diagnostics, environmental monitoring, and control of therapeutic microbes.

scholarly journals Programmable receptors enable bacterial biosensors to detect pathological biomarkers in clinical samples

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Hung-Ju Chang ◽  
Ana Zúñiga ◽  
Ismael Conejero ◽  
Peter L. Voyvodic ◽  
Jerome Gracy ◽  
...  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Clinical Samples ◽  
Monitoring And Control ◽  
Modular Assembly ◽  
E Coli ◽  
Naked Eye ◽  
Synthetic Receptor ◽  
And Control ◽  
Do So

AbstractBacterial biosensors, or bactosensors, are promising agents for medical and environmental diagnostics. However, the lack of scalable frameworks to systematically program ligand detection limits their applications. Here we show how novel, clinically relevant sensing modalities can be introduced into bactosensors in a modular fashion. To do so, we have leveraged a synthetic receptor platform, termed EMeRALD (Engineered Modularized Receptors Activated via Ligand-induced Dimerization) which supports the modular assembly of sensing modules onto a high-performance, generic signaling scaffold controlling gene expression in E. coli. We apply EMeRALD to detect bile salts, a biomarker of liver dysfunction, by repurposing sensing modules from enteropathogenic Vibrio species. We improve the sensitivity and lower the limit-of-detection of the sensing module by directed evolution. We then engineer a colorimetric bactosensor detecting pathological bile salt levels in serum from patients having undergone liver transplant, providing an output detectable by the naked-eye. The EMeRALD technology enables functional exploration of natural sensing modules and rapid engineering of synthetic receptors for diagnostics, environmental monitoring, and control of therapeutic microbes.

scholarly journals Detection of Antimicrobial Residues in Poultry Litter: Monitoring a Risk through a Selective and Sensitive HPLC–MS/MS Method

Animals ◽  
2021 ◽  
Vol 11 (5) ◽  
pp. 1399
Author(s):  
Karina Yévenes ◽  
Ekaterina Pokrant ◽  
Lina Trincado ◽  
Lisette Lapierre ◽  
Nicolás Galarce ◽  
...  
Keyword(s):  
High Performance ◽  
Limit Of Detection ◽  
Poultry Litter ◽  
Chromatography Tandem Mass Spectrometry ◽  
Antimicrobial Residues ◽  
Field Samples ◽  
Liquid Chromatography Tandem Mass ◽  
Commercial Poultry ◽  
Agriculture Fertilizer ◽  
And Control

Tetracyclines, sulphonamides, and quinolones are families of antimicrobials (AMs) widely used in the poultry industry and can excrete up to 90% of AMs administrated, which accumulate in poultry litter. Worryingly, poultry litter is widely used as an agriculture fertilizer, contributing to the spread AMs residues in the environment. The aim of this research was to develop a method that could simultaneously identify and quantify three AMs families in poultry litter by high-performance liquid chromatography–tandem mass spectrometry (HPLC–MS/MS). Samples of AMs free poultry litter were used to validate the method according to 657/2002/EC and VICH GL49. Results indicate that limit of detection (LOD) ranged from 8.95 to 20.86 μg kg−1, while limits of quantitation (LOQ) values were between 26.85 and 62.58 µg kg−1 of tetracycline, 4-epi-tetracycline, oxytetracycline, 4-epi-oxytetracycline, enrofloxacin, ciprofloxacin, flumequine, sulfachloropyridazine, and sulfadiazine. Recoveries obtained ranged from 93 to 108%. The analysis of field samples obtained from seven commercial poultry flocks confirmed the adequacy of the method since it detected means concentrations ranging from 20 to 10,364 μg kg−1. This provides us an accurate and reliable tool to monitor AMs residues in poultry litter and control its use as agricultural fertilizer.

scholarly journals Polyaniline Nanocomposites for Hydrogen Sulfide Detection at ppb Level

2021 ◽  
Vol 6 (1) ◽  
pp. 69
Author(s):  
Caroline Duc ◽  
Mohamed-Lamine Boukhenane ◽  
Thomas Fagniez ◽  
Nathalie Redon ◽  
Jean-Luc Wojkiewicz
Keyword(s):  
Hydrogen Sulfide ◽  
Tin Oxide ◽  
High Performance ◽  
Limit Of Detection ◽  
Anthropogenic Sources ◽  
Hazardous Substance ◽  
Repeatability And Reproducibility ◽  
New Sensors ◽  
And Control ◽  
Ppm Level

Coming from natural and anthropogenic sources, hydrogen sulfide gas (H2S) is a smelly hazardous substance at the sub-ppm level, which can lead to poisoning deaths at higher concentrations. New sensors with high metrological properties (detection limit lower than 1 ppm) and good stability are still needed to monitor and control the risk associated with this gas. The properties of a high-performance hydrogen sulfide gas sensor based on tin oxide and conductive polymers (polyaniline and poly(3,4-ethylenedioxythiophene):polystyrene sulfonate (PEDOT:PSS) are investigated. The principle of detection of this resistive sensor consists of a two steps reaction. H2S reacts with tin oxide producing hydrochloride acid, which dopes polyaniline, leading to the increase of its conductivity. Those systems present high repeatability and reproducibility, with sensitivities around 10%/ppm and a limit of detection close to 30 ppb. Moreover, the effect of interfering species such as humidity and oxidative gases (ammonia) is addressed. Those species have a limited impact, corrigible by data treatment. Finally, the sensors present an increase of sensitivity with time, apparently due to the modification of the interface between the electrodes and the sensitive materials.

scholarly journals Reconfigurable Multiple Sensing Node for the Automation of Agricultural Field

2019 ◽  
Vol 8 (10) ◽  
pp. 27-31
Keyword(s):  
High Performance ◽  
Low Cost ◽  
Computing System ◽  
Monitoring And Control ◽  
Agricultural Field ◽  
Real Time System ◽  
Agricultural Applications ◽  
High Yields ◽  
External Interface ◽  
And Control

In a real time system, monitoring and control of various parameters of the field is vital .In order to achieve high yields and quality , exact parameters of soils and its necessary inputs to soil need to be put in action. Pest and diseases are also important factors in decline of yield and quality. Considering the various applications of this area, the present paper explains the wireless multi-sensing node for remote monitoring and control system for agricultural applications. This is design with a number of good performance front-end devices and circuits suitable for various types of sensors. Any Sensor device elements can be directly connected without the need of extra circuits. The number of input sensors can be reconfigured with time to time as system demand. The paper describes development and the interface of high performance and low-cost typical features elements. The developed system displayed all the measures field parameters and corresponding set point values on the LCD interface and also are stored in external interface memory for future reference. The developed node can be connected to a personal computing system for decision support using wired RS-232 interface or wireless connectivity using the RF modem from Xbee.

scholarly journals Monitoring and control of E. coli cell integrity

2021 ◽  
Vol 329 ◽  
pp. 1-12
Author(s):  
Jens Kastenhofer ◽  
Vignesh Rajamanickam ◽  
Julian Libiseller-Egger ◽  
Oliver Spadiut
Keyword(s):  
Coli Cell ◽  
Cell Integrity ◽  
Monitoring And Control ◽  
E Coli ◽  
And Control

scholarly journals Development of RFID Based Smart Sensor Prototype for Wireless Industrial Monitoring and Control

2011 ◽  
pp. 176-181
Author(s):  
Urmila Deshmukh ◽  
R. N. Moghe
Keyword(s):  
Radio Frequency Identification ◽  
High Performance ◽  
Data Availability ◽  
Prototype System ◽  
Monitoring And Control ◽  
Wireless Technologies ◽  
Rfid Tag ◽  
Passive Tags ◽  
Industrial Monitoring ◽  
And Control

The Purpose of this paper is to present one of the various wireless technologies currently available for industrial monitoring and control. Applications of wireless data transmission are universal. In industrial automation, the benefits of adopting wireless technologies in eliminating the needs for cables in hard to reach areas within the plant, increasing data availability and quality and monitoring and controlling remote assets, that otherwise were inaccessible. Radio frequency identification (RFID) technology is commonly used for object or animal identification and tracking. This article explores the feasibility of its use in a rapid solution to wireless real time monitoring of industry. A prototype system for wireless industrial monitoring and control was developed using a commercially available 12.5 GHZ RFID passive tags. Various parameters are sensed by respective sensors (Slaves), which are then monitored by low power, high performance, 8bit AVR microcontroller. Monitored signals are then sent to the RFID tag or transponder unit, hence the smart feature of the sensor. A receiving unit (Interrogator) emits an electromagnetic field which when detected by passive RFID tag causes it to transfer sensor information (data stored in memory) to the interrogator. Interrogator detects these parameters and sends them to the data collection PC (Master Unit). The architecture of the developed wireless sensor prototype allows for additional RFID tags (Slave Units) to be integrated into it without changes to the sensor designs. Design also provides means to update operating and monitoring parameters as well as sensors/RF link specific firmware modules ‘over - the - air’.

scholarly journals LoRa (Long-Range) High-Density Sensors for Internet of Things

2019 ◽  
Vol 2019 ◽  
pp. 1-9 ◽  
Author(s):  
Alexandru Lavric
Keyword(s):  
Internet Of Things ◽  
Smart City ◽  
High Performance ◽  
Large Scale ◽  
Geographic Area ◽  
High Density ◽  
Monitoring And Control ◽  
Spreading Factor ◽  
Transfer Of Information ◽  
And Control

Over the past few years, there has been a growing awareness regarding the concept of Internet of Things (IoT), which involves connecting to the Internet various objects surrounding us in everyday life. The main purpose of this concept closely connected to the smart city issue is increasing the quality of life by contributing to streamlining resource consumption and protecting the environment. The LoRa communication mechanism is a physical layer of the LoRaWAN protocol, defined by the LoRa Alliance. Compared to other existing technologies, LoRa is a modulation technique enabling the transfer of information over a range of tens of kilometers. The main contribution this paper brings to the field is analyzing the scalability of the LoRa technology and determining the maximum number of sensors which can be integrated into this type of monitoring and control architecture. The sensor architecture is specific to the smart city concept that involves the integration of a large number of high-density sensors distributed on a large-scale geographic area. The reason behind this study is the need to assess the scalability of the LoRa technology, taking into consideration other factors, such as the packet payload size, the duty circle parameter, the spreading factor, and the number of nodes. The experimental results reveal that the maximum number of LoRa sensors that can communicate on the same channel is 1,500; furthermore, in order to obtain a high performance level, it is necessary to schedule and plan the network as carefully as possible. The spreading factor must be allocated according to the distance at which the sensor is placed from the gateway.

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