scholarly journals Integrated Genetic and Phenotypic Analysis for the Capsulars from Different Serotype of Campylobacter jejuni

2021 ◽  
Author(s):  
Jiaqi Wang ◽  
Xiaoli Chen ◽  
Guilan Zhou ◽  
Yixin Gu ◽  
Hairui Wang ◽  
...  
Keyword(s):  
Campylobacter Jejuni ◽  
Genome Sequencing ◽  
Capsular Polysaccharide ◽  
Draft Genome ◽  
Agglutination Reaction ◽  
Phenotypic Characteristics ◽  
Phenotypic Analysis ◽  
Genotypic Analysis ◽  
Draft Genome Sequencing ◽  
Different Strains

Abstract Background: Serotyping is one of the important typing scheme for Campylobacter jejuni (C. jejuni). However, this method is time-consuming and labor-intensive. Nowadays, genotypic analysis of the capsular polysaccharide (CPS) loci for the different serotype strains has been developed based on the unique sequences in specific serotypes. In order to establish the correlation between the genotypes and serotype characteristics, the genetic and phenotypic analysis was conducted in this study. Results: Draft-genome sequencing, followed by PCR, was performed to obtain the integrated CPS loci. Totally, the CPS loci from 26 C. jejuni isolates belonging to 9 genotypes were obtained, of which 15 serotypeable strains belonging to 10 serotypes. The main genetic polymorphisms of CPS loci are substitutions and variations in poly-G or poly-A tracts from the same genotype or serotype strains. A serotypic analysis was performed with different antigens, including the whole cell lysates, CPS and lipooligosaccharide (LOS), extracted from different strains. Compared to the results of the agglutination reaction with antiserum, similar typing results could be obtained using the CPS extract and LOS extracts did not cause serotype-specific passive agglutination.Conclusion: The genotyping results based on unique sequences were not always consistent with the phenotypic characteristics. The genotyping method based on unique sequences could not replace the serotyping but might be a supplement for genotyping of the non-serotypeable strains. Moreover, the present study supported that the determinant substance for Penner serotyping scheme is the CPS from C. jejuni instead of LOS.

scholarly journals Species Diversity of Dickeya and Pectobacterium Causing Potato Blackleg Disease in Pakistan

Plant Disease ◽  
2020 ◽  
Vol 104 (5) ◽  
pp. 1492-1499 ◽  
Author(s):  
Sohaib Sarfraz ◽  
Shahbaz Talib Sahi ◽  
Saïd Oulghazi ◽  
Kashif Riaz ◽  
Nasir Ahmed Rajput ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Dna Hybridization ◽  
Disease Incidence ◽  
Draft Genome ◽  
Carbon Sources ◽  
Housekeeping Genes ◽  
Blackleg Disease ◽  
Phenotypic Characteristics ◽  
Potato Blackleg ◽  
Draft Genome Sequencing

Potato blackleg is caused by a diverse species of pectinolytic bacteria. In Pakistan, approximately 90% of the pathogens involved belong to Pectobacterium atrosepticum. Survey (2014 to 2017), sampling, and isolation from different potato growing areas of Punjab, Pakistan depicted an overall disease incidence of approximately 15%. Thirty-six pectinolytic strains confirmed through biochemical and pathogenicity testing were characterized via gapA gene to identify them at the species level. To further validate the identification, one strain from each species SS26 (P. atrosepticum), SS28 (Pectobacterium polaris), SS70 (Dickeya dianthicola), SS90 (Pectobacterium parmentieri), SS95 (Pectobacterium punjabense), and SS96 (Pectobacterium versatile) were selected for draft genome sequencing and multilocus sequence analysis of 13 housekeeping genes (fusA, rpoD, acnA, purA, gyrB, recA, mdh, mtlD, groEL, secY, glyA, gapA, and rplB). Phylogenetic analysis revealed considerable genetic diversity in the genus Pectobacterium. In silico DNA–DNA hybridization and average nucleotide identity values of the strains selected for genome sequencing were determined with other reference Pectobacterium and Dickeya strains. Moreover, all six representative strains were also phenotypically characterized on the basis of metabolism of different carbon sources. Overall, on the basis of genotypic and phenotypic characteristics, these 36 isolates were grouped into six species: P. atrosepticum, P. versatile, P. parmentieri, P. polaris, P. punjabense, and D. dianthicola.

Draft genome sequencing of Sporolactobacillus terrae SBT-1, an efficient bacterium to ferment concentrated sugar to d-lactic acid

2021 ◽  
Author(s):  
Sitanan Thitiprasert ◽  
Jirabhorn Piluk ◽  
Vasana Tolieng ◽  
Naoto Tanaka ◽  
Yuh Shiwa ◽  
...  
Keyword(s):  
Lactic Acid ◽  
Genome Sequencing ◽  
Draft Genome ◽  
Draft Genome Sequencing

scholarly journals Draft Genome Sequences of Lysostaphin-Resistant (K07-204) and Lysostaphin-Susceptible (K07-561) Staphylococcus aureus Sequence Type 72 Strains Isolated from Patients in South Korea

2020 ◽  
Vol 9 (49) ◽  
Author(s):  
Nayab Batool ◽  
Kwan Soo Ko ◽  
Akhilesh Kumar Chaurasia ◽  
Kyeong Kyu Kim
Keyword(s):  
Staphylococcus Aureus ◽  
South Korea ◽  
Genome Sequencing ◽  
Draft Genome ◽  
Sequence Type ◽  
Genome Sequences ◽  
Content Type ◽  
Subtractive Genomics ◽  
Draft Genome Sequencing ◽  
Different Levels

ABSTRACT Methicillin-resistant Staphylococcus aureus sequence type 72 (ST72) is prevalent in South Korea and has shown resistance to multiple antimicrobials. ST72 isolates display different levels of resistance to the antistaphylococcal lysostaphin. Draft genome sequencing of ST72 human isolates exhibiting lysostaphin resistance or susceptibility was performed to better understand the mechanism of lysostaphin resistance using subtractive genomics.

scholarly journals First draft genome sequencing of the Salicola genus

2019 ◽  
Author(s):  
Lobna DAOUD ◽  
Adel HADJ BRAHIM ◽  
Houda HMANI ◽  
Mouna JLIDI ◽  
Manel BEN ALI ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Draft Genome ◽  
Draft Genome Sequencing

scholarly journals Draft Genome Sequencing of Pseudoalteromonas tetraodonis Strain kknpp56, a Potent Biofilm-Forming Bacterium Isolated from Early-Stage Marine Biofilm

2021 ◽  
Vol 10 (38) ◽  
Author(s):  
Meora Rajeev ◽  
T. J. Sushmitha ◽  
Subba Rao Toleti ◽  
Shunmugiah Karutha Pandian
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Complete Genome ◽  
Marine Bacterium ◽  
Early Stage ◽  
Draft Genome ◽  
Whole Genome ◽  
Content Type ◽  
Marine Biofilm ◽  
Draft Genome Sequencing

Pseudoalteromonas tetraodonis strain kknpp56 is an exopolysaccharide (EPS)-producing marine bacterium that forms potent biofilm. To determine the biosynthesis pathways involved in the EPS production of this bacterium, whole-genome sequencing was performed. The complete genome comes from one chromosome containing 3.72 Mbp of DNA with a G+C content of 41%.

Draft Genome Sequencing of Rhizoctonia solani Anastomosis Group 3 (AG3- PT) Causing Stem Canker and Black Scurf of Potato

2017 ◽  
Vol 95 (1) ◽  
pp. 87-91 ◽  
Author(s):  
Virupaksh U. Patil ◽  
Vanishree Girimalla ◽  
Vinay Sagar ◽  
Vinay Bhardwaj ◽  
S. K. Chakrabarti
Keyword(s):  
Rhizoctonia Solani ◽  
Genome Sequencing ◽  
Draft Genome ◽  
Stem Canker ◽  
Anastomosis Group ◽  
Black Scurf ◽  
Group 3 ◽  
Draft Genome Sequencing

scholarly journals Draft Genome Sequence ofBacillus licheniformisS127, Isolated from a Sheep Udder Clinical Infection

2015 ◽  
Vol 3 (5) ◽  
Author(s):  
Ievgenia Ostrov ◽  
Noa Sela ◽  
Mor Freed ◽  
Nihaya Khateb ◽  
Miriam Kott-Gutkowski ◽  
...  
Keyword(s):  
Genome Sequencing ◽  
Genome Sequence ◽  
Bacillus Licheniformis ◽  
Dairy Products ◽  
Draft Genome ◽  
Dairy Industry ◽  
Genetic Data ◽  
Draft Genome Sequence ◽  
Clinical Infection ◽  
Draft Genome Sequencing

Bacillus licheniformisis a Gram-positive biofilm- and endospore-forming bacterium, which contaminates dairy products and can be pathogenic to humans. The draft genome sequencing forB. licheniformisstrain S127 is reported here, providing genetic data relevant to the ability of this strain to sustain its survival in the dairy industry.

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