scholarly journals Bt cotton seed purity in Burkina Faso: Status and lessons learnt

2020 ◽  
Author(s):  
Larbouga Bourgou ◽  
Ester Kargougou ◽  
Mahamadou Sawadogo ◽  
Michel Fok
Keyword(s):  
Burkina Faso ◽  
Seed Production ◽  
Minor Role ◽  
Bt Cotton ◽  
Cotton Production ◽  
Cry1ac Gene ◽  
Organic Cotton ◽  
Production Scheme ◽  
Cotton Seeds ◽  
Varietal Purity

Abstract [Background] Since the commercial release of Bt cotton in Burkina Faso in 2009, the issue of seed purity in producers' fields has rarely been addressed in an unbiased and objective manner. The potential for contamination of conventional seed varieties with Bt traits, and the consequent threat to the continuation of organic cotton production has been documented. However, studies are rare on the varietal purity of Bt cotton seeds, despite the implications for the effectiveness and sustainability of their use. This paper compensates for the lack of research on the varietal purity of cotton seeds in Burkina Faso by reporting the results of ELISA tests collected in 2015 on samples of both conventional and Bt varieties from 646 fields. [Results] According to the conservative criteria used to declare the presence of a Bt gene in a given variety (more than 10% of seeds of conventional variety exhibit Bt traits, and at least 90% of seeds of Bt variety exhibit Bt traits) seed purity was very questionable for both types of variety. For the supposedly conventional variety, the Cry1Ac gene was observed in 63.6% of samples, the Cry2Ab gene was observed in 59.3% of samples, and both genes were detected in 52.2% of the seed samples. Only 29.3% of the seeds that were supposed to be conventional contained no Bt genes. Conversely, for the labeled Bt variety, the Cry1Ac gene was found in only 59.6% of samples, the Cry2Ab gene was found in 53.6% of the samples, and both genes were found in 40.4% of the samples. Finally, for the seeds that were supposed to contain both genes (Bollguard 2), both Cry1Ac and Cry2Ab genes were found in only 40.4% of the samples, only one of the genes was found in 32.4% of the samples, and 27.2% of the seeds in the samples contained neither gene. Two factors are responsible for the severe lack of seed purity. First, conventional varieties are being contaminated with Bt traits because of a failure to revise the seed production scheme in Burkina Faso to prevent cross fertilization. Second, the original Bt seeds provided to Burkina Faso lacked varietal purity. The organic sector plays a very minor role in the cotton sector of Burkina Faso (production of organic cotton totaled 453 tonnes in 2018/19, out of national cotton production of 183,000 tonnes). Nevertheless, the lack of purity in conventional seed varieties is a threat to efforts to expand certified organic cotton production. The poor expression of Bt traits in supposedly Bt varieties undermines their effectiveness in controlling pests and increases the likelihood of the development of resistance among pest populations. [Conclusion] Our results show the extent of purity loss when inadequate attention is paid to the preservation of seed purity. Pure conventional seeds could totally vanish in Burkina Faso, while Bt seeds do not carry the combination of the expected Bt traits. Any country wishing to embark on the use of Bt cotton, or to resume its use, as in the case of Burkina Faso, must first adjust its national seed production scheme to ensure that procedures to preserve varietal purity are enforced. The preservation of varietal purity is necessary to enable the launch or the continuation of identity-cotton production. In addition, the preservation of varietal purity is necessary to ensure the sustainable effectiveness of Bt cotton. In order to ensure that procedures to preserve varietal purity are observed, seed purity must be tested regularly, and test results must be published.

scholarly journals Bt cotton seed purity in Burkina Faso: status and lessons learnt

2020 ◽  
Vol 3 (1) ◽  
Author(s):  
Larbouga BOURGOU ◽  
Ester KARGOUGOU ◽  
Mahamadou SAWADOGO ◽  
Michel FOK
Keyword(s):  
Burkina Faso ◽  
Seed Production ◽  
Enzyme Linked Immunosorbent Assay ◽  
Minor Role ◽  
Bt Cotton ◽  
Cotton Production ◽  
Organic Cotton ◽  
Production Scheme ◽  
Cotton Seeds ◽  
Varietal Purity

Abstract Background Since the commercial release of Bt cotton in Burkina Faso in 2009, the issue of seed purity in producers’ fields has rarely been addressed in an unbiased and objective manner. The potential for contamination of conventional seed varieties with Bt traits and the consequent threat to the continuation of organic cotton production has been documented. However, studies are rare on the varietal purity of Bt cotton seeds, despite the implications for the effectiveness and sustainability of their use. This paper compensates for the lack of research on the varietal purity of cotton seeds in Burkina Faso by reporting the results of Enzyme linked immunosorbent assay tests collected in 2015 on samples of both conventional and Bt varieties from 646 fields. Results According to the conservative criteria used to declare the presence of a Bt gene in a given variety (more than 10% of seeds of conventional variety exhibit Bt traits, and at least 90% of seeds of Bt variety exhibit Bt traits), seed purity was very questionable for both types of variety. For the supposedly conventional variety, the Cry1Ac gene was observed in 63.6% of samples, the Cry2Ab gene was observed in 59.3% of samples, and both genes were detected in 52.2% of the seed samples. Only 29.3% of the seeds that were supposed to be of conventional type contained no Bt genes. Conversely, for the labeled Bt variety, the Cry1Ac gene was found in only 59.6% of samples, the Cry2Ab gene was found in 53.6% of the samples, and both genes were found in 40.4% of the samples. Finally, for the seeds that were supposed to contain both genes (Bollguard 2), both Cry1Ac and Cry2Ab genes were found in only 40.4% of the samples, only one of the genes was found in 32.4% of the samples, and 27.2% of the seeds in the samples contained neither. Two factors are responsible for the severe lack of seed purity. First, conventional varieties are being contaminated with Bt traits because of a failure to revise the seed production scheme in Burkina Faso to prevent cross-pollination. Second, the original Bt seeds provided to Burkina Faso lacked varietal purity. The organic sector plays a very minor role in the cotton sector of Burkina Faso (production of organic cotton totaled 453 t in 2018/2019, out of national cotton production of 183 000 t). Nevertheless, the lack of purity in conventional seed varieties is a threat to efforts to expand certified organic cotton production. The poor presence of Bt proteins in supposed Bt varieties undermines their effectiveness in controlling pests and increases the likelihood of the development of resistance among pest populations. Conclusion Our results show the extent of purity loss when inadequate attention is paid to the preservation of seed purity. Pure conventional seeds could vanish in Burkina Faso, while Bt seeds do not carry the combination of the expected Bt traits. Any country wishing to embark on the use of Bt cotton, or to resume its use, as in the case of Burkina Faso, must first adjust its national seed production scheme to ensure that procedures to preserve varietal purity are enforced. The preservation of varietal purity is necessary to enable the launch or the continuation of identity-cotton production. In addition, the preservation of varietal purity is necessary to ensure the sustainable effectiveness of Bt cotton. In order to ensure that procedures to preserve varietal purity are observed, seed purity must be tested regularly, and test results must be published.

scholarly journals Bt cotton seed purity in Burkina Faso: Status and lessons learnt

2020 ◽  
Author(s):  
Larbouga Bourgou ◽  
Ester Kargougou ◽  
Mahamadou Sawadogo ◽  
Michel Fok
Keyword(s):  
Burkina Faso ◽  
Seed Production ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bt Cotton ◽  
Cotton Production ◽  
Cry1ac Gene ◽  
Organic Cotton ◽  
Production Scheme ◽  
Cotton Seeds ◽  
Varietal Purity

Abstract BackgroundSince the commercial release of Bt cotton in Burkina Faso in 2009, the issue of seed purity in producers' fields has rarely been addressed in an unbiased and objective manner. The potential for contamination of conventional seed varieties with Bt traits and the consequent threat to the continuation of organic cotton production has been documented. However, studies are rare on the varietal purity of Bt cotton seeds, despite the implications for the effectiveness and sustainability of their use.This paper compensates for the lack of research on the varietal purity of cotton seeds in Burkina Faso by reporting the results of Enzyme linked immunosorbent assay tests collected in 2015 on samples of both conventional and Bt varieties from 646 fields.ResultsAccording to the conservative criteria used to declare the presence of a Bt gene in a given variety (more than 10% of seeds of conventional variety exhibit Bt traits, and at least 90% of seeds of Bt variety exhibit Bt traits), seed purity was very questionable for both types of variety. For the supposedly conventional variety, the Cry1Ac gene was observed in 63.6% of samples, the Cry2Ab gene was observed in 59.3% of samples, and both genes were detected in 52.2% of the seed samples. Only 29.3% of the seeds that were supposed to be of conventional type contained no Bt genes. Conversely, for the labeled Bt variety, the Cry1Ac gene was found in only 59.6% of samples, the Cry2Ab gene was found in 53.6% of the samples, and both genes were found in 40.4% of the samples. Finally, for the seeds that were supposed to contain both genes (Bollguard 2), both Cry1Ac and Cry2Ab genes were found in only 40.4% of the samples, only one of the genes was found in 32.4% of the samples, and 27.2% of the seeds in the samples contained neither.Two factors are responsible for the severe lack of seed purity. First, conventional varieties are being contaminated with Bt traits because of a failure to revise the seed production scheme in Burkina Faso to prevent cross-pollination. Second, the original Bt seeds provided to Burkina Faso lacked varietal purity.The organic sector plays a very minor role in the cotton sector of Burkina Faso (production of organic cotton totaled 453 tonnes in 2018/2019, out of national cotton production of 183 000 tonnes). Nevertheless, the lack of purity in conventional seed varieties is a threat to efforts to expand certified organic cotton production. The poor presence of Bt proteins in supposedly Bt varieties undermines their effectiveness in controlling pests and increases the likelihood of the development of resistance among pest populations.ConclusionOur results show the extent of purity loss when inadequate attention is paid to the preservation of seed purity. Pure conventional seeds could vanish in Burkina Faso, while Bt seeds do not carry the combination of the expected Bt traits. Any country wishing to embark on the use of Bt cotton, or to resume its use, as in the case of Burkina Faso, must first adjust its national seed production scheme to ensure that procedures to preserve varietal purity are enforced. The preservation of varietal purity is necessary to enable the launch or the continuation of identity-cotton production. In addition, the preservation of varietal purity is necessary to ensure the sustainable effectiveness of Bt cotton. In order to ensure that procedures to preserve varietal purity are observed, seed purity must be tested regularly, and test results must be published.

scholarly journals Bt cotton seed purity in Burkina Faso: Status and lessons learnt

2020 ◽  
Author(s):  
Larbouga Bourgou ◽  
Ester Kargougou ◽  
Mahamadou Sawadogo ◽  
Michel Fok
Keyword(s):  
Burkina Faso ◽  
Seed Production ◽  
Bt Cotton ◽  
Cotton Production ◽  
Organic Cotton ◽  
Related Information ◽  
Production Scheme ◽  
Bt Genes ◽  
Cotton Seeds ◽  
Bt Gene

Abstract BackgroundSince the commercial release of Bt cotton the issue of seed purity in producers' fields has been little addressed and in an unbalanced way when it was. It is well documented that the loss of purity in conventional seeds has endangered the continuation of organic cotton production. However, studies are rare on the purity of Bt-cotton seeds despite its implications on the effectiveness and sustainability of their use.This paper compensates for the mentioned lack of literature by analyzing data collected in 2015 in Burkina Faso, namely results of ELISA tests on samples of seeds from 646 fields grown with conventional or Bt varieties. ResultsAccording to the conservative criteria retained to declare the presence of Bt gene (more than 10% and 90% of controlled seeds for conventional and Bt variety, respectively), seed purity was very questionable for both types of varieties. For the conventional variety, the presence of Bt gene was observed on 63.6 and 59.3% of samples for Cry1Ac and Cry2Ab, respectively. Only 29.3% of samples corresponded to pure conventional seeds while 52.2% were double Bt seeds. Conversely, for the Bt variety, the presence of Bt gene was observed on 59.6 and 53.6% of samples for Cry1Ac and Cry2Ab, respectively. Actually BG2 seeds with both Bt genes were found in 40.4% of samples against 27.2% of samples of actually conventional seeds while the remaining of 32.4% of samples corresponded to single Bt gene seeds.Two factors affected the severe lack of seed purity. As regard to conventional seeds, it clearly resulted from a phenomenon of contamination, indicative of a failure in adjusting the seed production scheme to the use of Bt-cotton. With regard to the Bt variety, the lack of purity of the original seeds provided to Burkina Faso accounted and should even be the major factor.The observed lack of seed purity is a threat to the initiative of organic cotton production, albeit a very minor production mode in the country. It also calls upon the effectiveness and furthermore the sustainability of Bt cotton to control target pests. ConclusionOur results show the extent of purity loss when no especial attention is paid to the preservation of seed purity. Pure conventional seeds could totally vanish while Bt seeds become a combination of seeds of various types encompassing or not the expected Bt genes.Any country willing to embark the use of Bt cotton, or to resume this use like Burkina Faso, must previously adjust its seed production scheme and enforce its operation. This is a condition to preserve pure seeds both to enable the launch or the continuation of identity-cotton production and to ensure a sustainable effectiveness of Bt-cotton. The mentioned condition implies that seed purity must be checked and the related information shared.

scholarly journals Extent of Bollworm and Sucking Pest Damage on Modern and Traditional Cotton Species and Potential for Breeding in Organic Cotton

2019 ◽  
Vol 11 (22) ◽  
pp. 6353 ◽  
Author(s):  
Vonzun ◽  
Messmer ◽  
Boller ◽  
Shrivas ◽  
Patil ◽  
...  
Keyword(s):  
Water Dynamics ◽  
Bt Cotton ◽  
Cotton Production ◽  
Damage Potential ◽  
Scoring Method ◽  
Time Points ◽  
Pest Damage ◽  
Organic Cotton ◽  
Organic Systems ◽  
Cotton Species

Resistance against cotton bollworm is one of the main arguments for the use of genetically modified (GM) Bt cotton around the globe. The use of GM is prohibited in organic systems and thus the remunerative value of organic cotton cultivation depends on effective bollworm control. In this study, we investigated the extent of bollworm and sucking pest damage in 68 different hybrid and varietal lines of Gossypium hirsutum and varietal lines of G. arboreum at two different locations with contrasting soil fertility and water dynamics. The damage potential of bollworms was assessed from open capsules at two time points. Sucking pests were assessed at three time points using a scoring method. G. arboreum varietal lines and G. hirsutum hybrids were on average significantly more tolerant than G. hirsutum varietal lines to bollworm under fertile and irrigated situations. For sucking pests, the G. arboreum varietal lines were clearly more tolerant than G. hirsutum hybrids and varietal lines. Since, recently, pink bollworm (Pectinophora gossypiella) became resistant against Bt cotton and pressure of sucking pests severely increased, screening of genetic resources and systems-based cotton breeding for bollworm and sucking pest tolerance will improve sustainability of organic and conventional cotton production.

scholarly journals Evaluation et sélection d’une variété de cotonnier (FK64, Gossypium hirsutum L.) au Burkina Faso

2020 ◽  
Vol 14 (3) ◽  
pp. 869-882
Author(s):  
Larbouga Bourgou ◽  
Windpouiré Vianney Tarpaga ◽  
Sidiki K. Diane ◽  
Denys Sanfo
Keyword(s):  
Gossypium Hirsutum ◽  
Burkina Faso ◽  
Fiber Quality ◽  
Bt Cotton ◽  
Market Demand ◽  
Cotton Production ◽  
Gossypium Hirsutum L ◽  
Seed Index ◽  
Production Areas ◽  
Multilocation Trials

Le coton du Burkina Faso est presqu’entièrement exporté ; les variétés cultivées doivent régulièrement répondre aux exigences du marché. Une fratrie de lignées de cotonnier a été comparée à deux variétés, FK37 et Stam 59A, respectivement dans les zones cotonnières humides et sèches de 2006 à 2009, pour mettre en exergue celle qui améliore les performances agronomiques et technologiques de fibre de ces témoins. A l’issue d’évaluations multilocales, FK64 et FK69 ont été identifiées comme de potentielles nouvelles variétés et testées en milieu paysan. Dans les zones humides, FK64 et FK69 n’ont pas apporté d’amélioration significative ; il est inopportun de les y vulgariser. Dans les zones sèches, FK64 a le mieux performé et a été retenue pour y être vulgarisée. Elle apporte un surplus en pourcentage fibre (+ 0,41%) et en seed index (+ 0,35 g). Aussi, elle apparait meilleure que Stam 59A pour la longueur (+ 1,44 mm) et la ténacité (+ 1,90 g/tex), deux caractéristiques déterminantes du marketing de la fibre. Avec la suspension du coton Bt et le retour à la culture conventionnelle, FK64 lancée en culture commerciale a convaincu. Elle pourrait être candidate à la transformation en cas de retour dans la technologie Bt.Mots clés: Evaluations multilocales, tests variétaux, caractéristiques agronomiques, caractéristiques technologiques, Burkina Faso.   English Title: Assessment and selection of a cultivar of cotton (FK64, Gossypium hirsutum L.) in Burkina Faso Cotton produced in Burkina Faso is almost entirely exported; so cultivars must be developed following the market demand. A sibling of cotton lines was compared to two varieties, FK37 and Stam 59A, respectively in humid and dry cotton production areas between 2006 and 2009 to highlight which one enhances agronomic and fiber quality performances compared to the local control. After multilocation trials, FK64 and FK69 were highlighted as promising news varieties then evaluated under farmer’s conditions of production. In the humid areas, FK64 as well as FK69 did not bring any significant improvement; it was not recommended to release any of them there. In the dry production areas, FK64 performed better, then it was retained to be possibly popularized. It brings a surplus in terms of ginning out turn (+ 0,41%) and seed index (+ 0,35 g). Moreover, it outperformed Stam 59A in fiber length (+ 1,44 mm) and strength (+ 1,90 g/tex), two major cotton fiber marketing properties. At the morrow of the suspension of the Bt cotton and back to conventional production, FK64 was launched in commercial production and it convinced. It could be aspirant to Bt introgression in case country resumes back to the technology.Keywords: multilocation trials, varietal trials, agronomical characteristics, fiber properties, Burkina Faso.

Broad-scale suppression of cotton bollworm, Helicoverpa armigera (Lepidoptera: Noctuidae), associated with Bt cotton crops in Northern New South Wales, Australia

2016 ◽  
Vol 107 (2) ◽  
pp. 188-199 ◽  
Author(s):  
G.H. Baker ◽  
C.R. Tann
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Cotton Bollworm ◽  
Bt Cotton ◽  
Cotton Production ◽  
Bt Toxin ◽  
Pheromone Trapping ◽  
Eastern Australia ◽  
Helicoverpa Armígera ◽  
Broad Scale

AbstractThe cotton bollworm, Helicoverpa armigera, is a major pest of many agricultural crops in several countries, including Australia. Transgenic cotton, expressing a single Bt toxin, was first used in the 1990s to control H. armigera and other lepidopteran pests. Landscape scale or greater pest suppression has been reported in some countries using this technology. However, a long-term, broad-scale pheromone trapping program for H. armigera in a mixed cropping region in eastern Australia caught more moths during the deployment of single Bt toxin cotton (Ingard®) (1996–2004) than in previous years. This response can be attributed, at least in part, to (1) a precautionary cap (30% of total cotton grown, by area) being applied to Ingard® to restrict the development of Bt resistance in the pest, and (2) during the Ingard® era, cotton production greatly increased (as did that of another host plant, sorghum) and H. armigera (in particular the 3rd and older generations) responded in concert with this increase in host plant availability. However, with the replacement of Ingard® with Bollgard II® cotton (containing two different Bt toxins) in 2005, and recovery of the cotton industry from prevailing drought, H. armigera failed to track increased host-plant supply and moth numbers decreased. Greater toxicity of the two gene product, introduction of no cap on Bt cotton proportion, and an increase in natural enemy abundance are suggested as the most likely mechanisms responsible for the suppression observed.

scholarly journals A discussion on cotton transformation during the last decade (2010–2021); an update on present trends and future prospects

2021 ◽  
Vol 4 (1) ◽  
Author(s):  
QANDEEL-E-ARSH ◽  
Muhammad Tehseen AZHAR ◽  
Rana Muhammad ATIF ◽  
Mahwish ISRAR ◽  
Azeem Iqbal KHAN ◽  
...  
Keyword(s):  
Herbicide Tolerance ◽  
Routine Activity ◽  
Fourth Generation ◽  
Bt Cotton ◽  
Gene Gun ◽  
Cotton Production ◽  
Cotton Transformation ◽  
Lepidopteran Insects ◽  
The Us ◽  
The World

AbstractThe introduction of genetically modified (GM) cotton in 1996 in the US and its worldwide spread later rejuvenated cotton production in many parts of the world. The evolution is continued since then and currently, the 3rd and fourth generation of same GM cotton is grown in many parts of the world. The GM cotton introduced in 1996 was simple Bt cotton that expressed a single Cry1Ac gene, the later generation carried multiple Cry genes along with the genes controlling herbicide tolerance. Current day GM cotton does not only give stable resistance against lepidopteran insects but also facilitates the farmers to spray broad-spectrum herbicides without harming the crop. The evolution of GM cotton is continued both on the basic and applied side and interventions have been introduced during the last decade. Earlier the cotton transformation was limited to Cocker strains which are getting possible in many other varieties, too. It is successful with both gene gun, and Agrobacterium and inplanta transformation has made it a routine activity. Apart from overexpression studies for various purposes including biotic, abiotic, and quality traits, RNAi and genome editing are explored vigorously. Through this review, we have tried to explore and discuss various interventions for improving transformation protocols, the applications of cotton transformation, and future strategies being developed to get maximum benefits from this technology during the last decade.

Varietal Identity and Varietal Purity. Viral and Viroid Pathogens Controlled in Potato Seed Production

2021 ◽  
pp. 87-93
Author(s):  
Evgeny Alekseevich Simakov ◽  
Elena Vasil’evna Oves ◽  
Sergey Nikolaevich Zebrin ◽  
Boris Vasil’evich Anisimov
Keyword(s):  
Seed Production ◽  
Potato Seed ◽  
Varietal Purity

scholarly journals Peasant essentialism in GMO debates: Bt cotton in Burkina Faso

2020 ◽  
Vol 20 (4) ◽  
pp. 579-597 ◽  
Author(s):  
Jessie K. Luna
Keyword(s):  
Burkina Faso ◽  
Bt Cotton

Mating of Helicoverpa armigera (Lepidoptera: Noctuidae) moths and their host plant origins as larvae within Australian cotton farming systems

2012 ◽  
Vol 103 (2) ◽  
pp. 171-181 ◽  
Author(s):  
G.H. Baker ◽  
C.R. Tann
Keyword(s):  
Host Plant ◽  
Helicoverpa Armigera ◽  
Stable Carbon Isotopes ◽  
Production Systems ◽  
Farming Systems ◽  
C4 Plants ◽  
C3 Plants ◽  
Bt Cotton ◽  
Cotton Production ◽  
Helicoverpa Armígera

AbstractTransgenic (Bt) cotton dominates Australian cotton production systems. It is grown to control feeding damage by lepidopteran pests such as Helicoverpa armigera. The possibility that these moths might become resistant to Bt remains a threat. Consequently, refuge crops (with no Bt) must be grown with Bt cotton to produce large numbers of Bt-susceptible moths to reduce the risk of resistance developing. A key assumption of the refuge strategy, that moths from different host plant origins mate at random, remains untested. During the period of the study reported here, refuge crops included pigeon pea, conventional cotton (C3 plants), sorghum or maize (C4 plants). To identify the relative contributions made by these (and perhaps other) C3 and C4 plants to populations of H. armigera in cotton landscapes, we measured stable carbon isotopes (δ13C) within individual moths captured in the field. Overall, 53% of the moths were of C4 origin. In addition, we demonstrated, by comparing the stable isotope signatures of mating pairs of moths, that mating is indeed random amongst moths of different plant origins (i.e. C3 and C4). Stable nitrogen isotope signatures (δ15N) were recorded to further discriminate amongst host plant origins (e.g. legumes from non-legumes), but such measurements proved generally unsuitable. Since 2010, maize and sorghum are no longer used as dedicated refuges in Australia. However, these plants remain very common crops in cotton production regions, so their roles as ‘unstructured’ refuges seem likely to be significant.

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