A Method of Improving Physical Adsorption of Cellulase On Mesoporous Zr-Based MOF Surface: Enhanced Stability, Recyclability, And Catalytic Efficiency

Abstract In this paper, we aimed at developing immobilized cellulase biocatalyst by enhancing the anchor of cellulase on support surface. A mesoporous Zr-based MOF was first synthesized by biomineralization method using dextran as template. The resultant PVP-cellulase@CD-UIO-66-Zr exhibited a high loading capacity of 265 mg g− 1 support. The physical adsorption of cellulase on CD-UIO-66-Zr could be further enhanced by the capping of cellulase with PVP. About 83% of the activity of PVP-cellulase@CD-UIO-66-Zr could be retained after six cycles, and its equilibrium leakage ratio was 36% during thirty days’ leaching test. It was noting that about 80% of activity of immobilized PVP-cellulase@CD-UIO-66-Zr could be retained after incubation at 80°C for 1 hour. The immobilized cellulase exhibited higher pH stability, thermostability, storage stability and catalytic efficiency than free one.

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Manipulating the surface-enhanced Raman spectroscopy (SERS) activity and plasmon-driven catalytic efficiency by the control of Ag NP/graphene layers under optical excitation

Nanophotonics ◽

10.1515/nanoph-2020-0644 ◽

2021 ◽

Vol 10 (5) ◽

pp. 1529-1540

Author(s):

Xianwu Xiu ◽

Liping Hou ◽

Jing Yu ◽

Shouzhen Jiang ◽

Chonghui Li ◽

...

Keyword(s):

Raman Spectroscopy ◽

Hot Spot ◽

Catalytic Efficiency ◽

Surface Enhanced Raman Spectroscopy ◽

In Situ Monitoring ◽

Excitation Beam ◽

Graphene Layers ◽

Cu Film ◽

Surface Enhanced ◽

Surface Enhanced Raman

Abstract Highly efficient plasmon-driven catalysis and excellent surface-enhanced Raman spectroscopy (SERS) performance are proportional to the square of the local electromagnetic field (hot spot). However, a proven way to realize the enhancement in intensity and density of “hot spot” still needs to be investigated. Here, we report on multilayered Ag nanoparticle (Ag NP)/graphene coupled to an underlying Cu film system (MAgNP-CuF) which can be used as an effective SERS substrates realizing ultra-sensitive detection for toxic molecules and in situ monitoring the plasmon-driven reaction for p-nitrothiophenol (PNTP) to p,p′-dimercaptobenzene (DMAB) conversion. The mechanism of ultra-sensitive SERS response and catalytic reaction is investigated via Ag NP/graphene layer-dependent experiments combined with theoretical simulations. The research found that the intensity and density of “hot spot” can be effectively manipulated by the number of plasmonic layers, and the bottom Cu film could also reflect the scattered and excitation beam and would further enhance the Raman signals. Moreover, the MAgNP-CuF exhibits outstanding performance in stability and reproducibility. We believe that this concept of multilayered plasmonic structures would be widely used not only in the field of SERS but also in the wider research in photocatalysis.

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Effects of morphology and pore size of mesoporous silicas on the efficiency of an immobilized enzyme

RSC Advances ◽

10.1039/d1ra01358k ◽

2021 ◽

Vol 11 (17) ◽

pp. 10010-10017

Author(s):

Ping-Chung Kuo ◽

Zhi-Xun Lin ◽

Tzi-Yi Wu ◽

Chun-Han Hsu ◽

Hong-Ping Lin ◽

...

Keyword(s):

Enzyme Activity ◽

Mesoporous Silica ◽

Pore Size ◽

Immobilized Enzyme ◽

Mesoporous Silicas ◽

High Loading ◽

Loading Capacity ◽

Silica Films

Mesoporous silica films were used as supports with high loading capacity and enzyme activity.

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Real time monitoring of peptide delivery in vitro using high payload pH responsive nanogels

Polymer Chemistry ◽

10.1039/c9py01120j ◽

2020 ◽

Vol 11 (2) ◽

pp. 425-432 ◽

Cited By ~ 2

Author(s):

Shegufta Farazi ◽

Fan Chen ◽

Henry Foster ◽

Raelene Boquiren ◽

Shelli R. McAlpine ◽

...

Keyword(s):

Real Time ◽

Intracellular Delivery ◽

Peptide Delivery ◽

High Loading ◽

Loading Capacity ◽

Ph Responsive ◽

Real Time Monitoring ◽

High Payload

A pH responsive pMAA nanogel that demonstrates high loading capacity and rapid intracellular delivery of hydrophilic peptides.

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Preparation of porous cellulose 3,5-dimethylphenylcarbamate hybrid organosilica particles for chromatographic applications

Journal of Materials Chemistry B ◽

10.1039/c4tb01547a ◽

2015 ◽

Vol 3 (4) ◽

pp. 620-628 ◽

Cited By ~ 17

Author(s):

Xilun Weng ◽

Zongbi Bao ◽

Zhiguo Zhang ◽

Baogen Su ◽

Huabin Xing ◽

...

Keyword(s):

Silica Particles ◽

Chiral Selector ◽

High Loading ◽

Loading Capacity ◽

Functionalized Silica

Organic–inorganic hybrids incorporating cellulose tris(3,5-dimethylphenylcarbamate) (CDMPC)-functionalized silica particles can exhibit a high loading capacity with high loadings of the chiral selector.

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Design and synthesis of periodic mesoporous organosilica materials with a multi-compartment structure

RSC Advances ◽

10.1039/c5ra16497d ◽

2015 ◽

Vol 5 (109) ◽

pp. 89397-89406 ◽

Cited By ~ 1

Author(s):

Chun Xiang Cynthia Lin ◽

Siddharth Jambhrunkar ◽

Pei Yuan ◽

Chun Hui Clayton Zhou ◽

George Xiu Song Zhao

Keyword(s):

Anticancer Drug ◽

Release Rate ◽

Slow Release ◽

Drug Carrier ◽

High Loading ◽

Loading Capacity ◽

Periodic Mesoporous Organosilica ◽

Design And Synthesis ◽

Mesoporous Organosilica ◽

Organosilica Materials

Multi-compartment periodic mesoporous organosilica materials show desirable properties as anticancer drug carrier with high loading capacity and slow release rate.

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