scholarly journals First Record of Traumatic Myiasis Obtained From Forest Musk Deer (Moschus Berezovskii) Using Dna Barcoding

2021 ◽  
Author(s):  
Yunyun Gao ◽  
Yajun Fu ◽  
Liping Yan ◽  
Defu Hu ◽  
Benmo Jiang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Haplotype Diversity ◽  
First Record ◽  
Nucleotide Composition ◽  
Musk Deer ◽  
Accurate Identification ◽  
Tree Construction ◽  
Nj Tree ◽  
Nucleotide Divergence ◽  
First Time

Abstract BackgroundMyiasis is an infestation of maggots on living tissue in humans and animals all over the world. It is known to occur in wild animals, while no information is reported in forest musk deer. We found a case of traumatic myiasis of an injured forest musk deer (Moschus berezovskii), the wound of which was infected by numerous maggots, and the fur was covered by clusters of eggs. The affected individual was clinically treated immediately and recovered. MethodsDNA barcoding is an efficient technique for species diagnosis, therefore is employed to identify the blowfly samples collected from the infected forest musk deer. Firstly, we extracted genomic DNA from larvae and eggs respectively. The DNA barcoding sequences of 49 individuals were obtained and subsequently analyzed calculating nucleotide composition and divergence and haplotypes, constructing a neighbor-joining (NJ) tree, for accurate identification of these blowflies. ResultsOur results suggest that the average nucleotide divergence between the 49 sequences of blowfly samples is 0.0022, 0.0054 is between sequences of blowfly samples and Lucilia caesar (Linnaeus, 1758) (Diptera: Calliphoridae). Furthermore, the NJ tree construction indicates that the flies collected from the forest musk deer are clustered together with L. caesar. The sequences of sampled blowflies have nine haplotypes, including two shared haplotypes, with haplotype diversity 0.588, nucleotide diversity 0.00215, and the average number of nucleotide differences was 1.374. ConclusionsWe report traumatic myiasis of forest musk deer for the first time, which expands the information on parasite and myiasis of forest musk deer and confirmed the potential risk of traumatic myiasis of forest musk deer.

scholarly journals First Record of Traumatic Myiasis Obtained From Forest Musk Deer (Moschus Berezovskii) Using DNA Barcoding

2020 ◽  
Author(s):  
Yunyun Gao ◽  
Yajun Fu ◽  
Liping Yan ◽  
Defu Hu ◽  
Benmo Jiang ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Animal Husbandry ◽  
First Record ◽  
Coi Gene ◽  
Common Disease ◽  
Human Society ◽  
Musk Deer ◽  
Two Samples ◽  
Nucleotide Divergence ◽  
Coi Sequences

Abstract Background: Myiasis is a common disease occurring in humans and livestock all over the world. It is closely related to human society and of great significance to animal husbandry, forensic science, and medicine. Myiasis is known to occur in wild animals, while no information is reported in forest musk deer. On July 6, 2019, we found severe traumatic myiasis of an injured forest musk deer (Moschus berezovskii) (Flerov, 1929), infected by lots of maggots and clusters of eggs. However, the precise identification of the larvae that we collected was difficult with few specific morphology characteristics.Methods: DNA barcoding is an efficient technique for species diagnosis, therefore is employed to identify the samples collected from the infected forest musk deer. Firstly, we extracted genomic DNA from one larva and one egg respectively. The cytochrome oxidase I (COI) gene barcoding region was amplified by polymerase chain reaction (PCR) and bidirectionally sequenced by Sanger sequencing. The sequences were searched for similarity using BLAST and the best hits were Lucilia. To identify these blowflies accurately, these newly generated sequences were subsequently analyzed with COI sequences of Lucilia and Calliphora downloaded from GenBank, to calculate nucleotide divergence, and to construct a neighbor-joining tree.Results: Our results suggest that nucleotide divergence between the two samples is 0.0033cM, between two samples and Lucilia caesar (Diptera: Calliphoridae) (Linnaeus, 1758) is 0.0016–0.0050cM. Furthermore, the NJ tree construction indicates that the flies collected from the musk deer are Lucilia caesar.Conclusions: Our results indicate that DNA barcoding can successfully identify pathogenic species. As far as the authors know, this is the first time that myiasis is detected in forest musk deer caused by a traumatic infection in China. The affected individual was clinically treated immediately and apparently recovered.

scholarly journals Research on Phylogenetic Relationship of Lotus Populations Collected in Thua Thien Hue Province, Vietnam based on the Chloroplast Genome by DNA Barcode

2021 ◽  
Author(s):  
Dang Thanh Long ◽  
Hoang Thi Kim Hong ◽  
Le Ly Thuy Tram ◽  
Nguyen Thi Quynh Trang
Keyword(s):  
Chloroplast Genome ◽  
Dna Barcoding ◽  
Phylogenetic Analyses ◽  
Dna Barcode ◽  
Haplotype Diversity ◽  
Universal Primers ◽  
Nucleotide Position ◽  
Accurate Identification ◽  
Low Levels ◽  
Relationship Of

Background: The DNA barcoding is currently an effective and widely used tool that enables rapid and accurate identification of plant species. Methods: DNA barcoding of 9 chloroplast genes (rbcL, matK, trnH-psbA, accD-psaI, ndhA, psbE-petL, Rpl32-trnL, trnW-psaJ, trnSGCU-trnGGCC) were used to provide the theoretical basis for species identification, genetic diversity analysis of lotus population collected in Thua Thien Hue province, Vietnam. Universal primers were used and sequence products were analyzed using the MEGA X program. Result: The results showed that high levels of haplotype diversity (Hd), ranging from 0.618-0.869 and low levels of nucleotide diversity (Pi), ranging from 0.180 × 10-3-3.280 × 10-3 base on a total of nine gene regions of chloroplast genome. The neutrality tests show an excess of rare nucleotide position variations in individuals’ white lotus and derived haplotypes recent expansion. While the evolution of the individuals in the pink lotus may have to decrease. The phylogenetic analyses indicated that combined sequences were not insufficient to make a difference to the DNA barcoding in the individual’s lotus of the N. nucifera species this is in the study. The standardized and accurate barcode information of lotus is provided for researchers. It lays the foundation for the conservation, evaluation, innovative utilization and protection of Nelumbonaceae germplasm resources.

scholarly journals DNA barcoding confirms the first record of a Desmodema polystictum (Ogilby, 1898) egg and all-time high adult catches in the Indian Ocean

2019 ◽  
Vol 12 (1) ◽  
Author(s):  
M. I. G. Rathnasuriya ◽  
A. Mateos-Rivera ◽  
A. G. G. C. Bandara ◽  
R. Skern-Mauritzen ◽  
R. P. P. K. Jayasinghe ◽  
...  
Keyword(s):  
Pacific Ocean ◽  
Indian Ocean ◽  
Dna Barcoding ◽  
First Record ◽  
Spawning Ground ◽  
Molecular Analyses ◽  
Line Drawings ◽  
Central Indian Ocean ◽  
The Indian Ocean ◽  
First Time

Abstract The eggs of Polka-dot ribbonfish Desmodema polystictum have been recorded for the first time in the Indian Ocean. Although the only previous information on eggs from this species consists of line drawings from 1973 (Pacific Ocean), the identification was possible by combining morphological and molecular analyses. As far as we are aware, only few confirmed records of adult individuals have been previously reported from the Indian Ocean. We found eggs in the proximity of numerous adults (57 and 42 individuals at two stations) indicating that the Central Indian Ocean is potentially an important spawning ground of D. polystictum.

scholarly journals Phylogeographic and molecular characterization of Pronghorn spiny lobster (Panulirus penicillatus Olivier, 1791) in the Southern Coast of Java and Lombok, Indonesia

2020 ◽  
Vol 21 (12) ◽  
Author(s):  
Irwani ◽  
Diah Permata Wijayanti ◽  
ANINDIA WIRA SATRIA ◽  
AGUS SABDONO
Keyword(s):  
Dna Barcoding ◽  
Molecular Characterization ◽  
Spiny Lobster ◽  
Taxonomic Status ◽  
Molecular Technique ◽  
Southern Coast ◽  
Central Java ◽  
Nucleotide Divergence ◽  
First Time

Abstract. Irwani, Wijayanti DP, Satria AW, Sabdono A. 2020. Phylogeographic and molecular characterization of Pronghorn spiny lobster (Panulirus penicillatus Olivier, 1791) in the Southern Coast of Java and Lombok, Indonesia. Biodiversitas 21: 5691-5697. Panulirus penicillatus is one of the most important lobster species in Central Java. It is one of the most populous species in the southern part of Java and Lombok. However, uncontrolled catching causes a decrease in the population of P. penicillatus. Despite such human threats, information about the taxonomic status of this species is limited. Several previous reports on the taxonomy of this species have always been based on the morphological features that cause ambiguous identification. Therefore, this study aimed to establish the relationships of P. penicillatus in southern parts of the southern part of Java and Lombok by using the molecular technique. Twelve samples were collected from four study areas of the southern coast of the southern part of Java and Lombok and identified using DNA barcoding technique. DNA barcoding technique was used for the first time to identify P. penicillatus in this region. This study demonstrated that all specimens belonged to P. penicillatus. However, one specimen (PPK-06) showed high intraspecies nucleotide divergence that formed a distinct subclade. Therefore, the specimen (PPK-06) could represent a cryptic species within P. penicillatus that needs to be studied further.

scholarly journals CO1-Based DNA barcoding for assessing diversity of Pteropus giganteus from the state of Azad Jammu Kashmir, Pakistan

2020 ◽  
Author(s):  
Sana Karamat ◽  
Nasra Ashraf ◽  
Tasleem Akhtar ◽  
Faisal Rahim ◽  
Nuzhat Shafi ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Haplotype Diversity ◽  
Nucleotide Composition ◽  
Neutral Evolution ◽  
Fruit Bat ◽  
Bioinformatics Software ◽  
Pcr Products ◽  
The Mean ◽  
Flying Fox

Abstract The flying fox (Pteropus giganteus) also familiar with the name of the greater Indian fruit Bat belongs to the order Chiroptera and family Pteropodidae. Current research emphasis on the DNA barcoding of P. giganteus in Azad Jammu Kashmir. Bat sequences were amplified and PCR products were sequenced and examined by bioinformatics software. Congeneric and conspecific, nucleotide composition and K2P nucleotide deviation, haplotype diversity and the number of haplotypes were estimated. The analysis showed that all of the five studied samples of P. giganteus had low G contents (G 19.8%) than C (27.8%), A (25.1%) and T (27.3%) contents. The calculated haplotype diversity was 0.60% and the mean intraspecific K2P distance was 0.001% having a high number of transitional substitutions. The study suggested that P. giganteus (R=0.00) do not deviate from the neutral evolution. It was determined from the conclusion that this mtDNA gene is a better marker for identification of Bat species than nuclear genes due to its distinctive characteristics and may serve as a landmark for the identification of interconnected species at the molecular level and in the determination of population genetics.

scholarly journals Hidden in our pockets: building of a DNA barcode library unveils the first record of Myotis alcathoe for Portugal

2020 ◽  
Vol 8 ◽  
Author(s):  
Hugo Rebelo ◽  
Sónia Ferreira ◽  
Francisco Amorim ◽  
Pedro Horta ◽  
Helena Raposeira ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Dna Sequences ◽  
Dna Barcode ◽  
First Record ◽  
The Other ◽  
Index Numbers ◽  
Vast Array ◽  
First Time ◽  
Nyctalus Noctula

The advent and boom of DNA barcoding technologies have provided a powerful tool for the fields of ecology and systematics. Here, we present the InBIO Barcoding Initiative Database: Portuguese Bats (Chiroptera) dataset containing DNA sequences of 63 specimens representing the 25 bat species currently known for continental Portugal. For that, we sequenced tissues samples obtained in a vast array of projects spanning the last two decades. We added four new Barcoding Index Numbers (BINs) to existing Chiroptera barcodes on BOLD, two belonging to Myotis escalerai, one to Plecotus auritus and the other to Rhinolophus hipposideros. Surprisingly, one of the samples initially identified in the field as Myotis mystacinus turned out to be Myotis alcathoe, which represents the first record of this species for Portugal. The presence of Nyctalus noctula in Portugal was also genetically confirmed for the first time. This case study shows the power and value of DNA barcoding initiatives to unravel new data that may be hidden on biological collections.

scholarly journals First record of Cyrnus crenaticornis (Kolenati, 1859) (Insecta, Trichoptera, Polycentropodidae) in Croatia: morphological determination and DNA barcoding

2021 ◽  
Vol 30 (2) ◽  
pp. 405-416
Author(s):  
Renata Ćuk ◽  
◽  
Mladen Kučinić ◽  
Lidija Kladarić ◽  
Dora Hlebec ◽  
...  
Keyword(s):  
Dna Barcoding ◽  
Larval Stage ◽  
Morphological Characteristics ◽  
First Record ◽  
Odra River ◽  
First Time

The caddisfly species Cyrnus crenaticornis (Kolenati, 1859) was recorded for the first time in Croatia in the Odra River during August 2015. The record refers to a larval stage which was determined according to morphological characteristics and supported by DNA barcoding.

First record of the superfamily Proctotrupoidea from French Polynesia (Hymenoptera)

2020 ◽  
Vol 125 (4) ◽  
pp. 404-406
Author(s):  
Thibault Ramage
Keyword(s):  
First Record ◽  
French Polynesia ◽  
Society Islands ◽  
First Time

A Helorid wasp, Helorus ruficornis Förster, 1856, is reported for the first time on Tahiti (Society Islands), which is also the first record of both Proctotrupoidea and family Heloridae in French Polynesia. The potential hosts of Helorus ruficornis in French Polynesia are discussed.

FIRST RECORD OF MANTIDS (MANTODEA: INSECTA) FROM INDIA

2018 ◽  
Vol 24 (1) ◽  
Author(s):  
SHVETA PATEL ◽  
RAJENDRA SINGH
Keyword(s):  
Uttar Pradesh ◽  
First Record ◽  
Target Area ◽  
Extensive Survey ◽  
First Time

Extensive survey of mantids in the northeastern Uttar Pradesh was conducted. Two mantid species were recorded for the first time from the target area, viz.: Pyrgomantis pallida, 1917 and Bactromantis mexicana.

First Record of Trichodina magna Van As and Basson, 1989 (Ciliophora: Trichodinidae) from Gills of Blue Tilapia Oreochromis aureus (Steindachner, 1864) in Iraq

2018 ◽  
pp. 59
Author(s):  
Kefah Naser Abdul-Ameer ◽  
Fatima Khalaf Atwan
Keyword(s):  
First Record ◽  
Oreochromis Aureus ◽  
Tigris River ◽  
Blue Tilapia ◽  
First Time

   The Ciliophoran Trichodina magna Van As and Basson, 1989 is recorded for the first time in in Iraq from gills of the blue tilapia Oreochromis aureus (Steindachner, 1864) Iraq from Al-Graiat location on the Tigris River at Baghdad city. The description and measurements of this external parasite as well as its illustrations are given.  

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