Detection of the Communication Site by Indocyanine Green Adsorbed to Human Serum Albumin Fluorescence During Surgery for a Pleuroperitoneal Communication

2021 ◽  
Author(s):  
Daisuke Kimura ◽  
Ikuo Fukuda ◽  
Takeshi Fujita ◽  
Reiichi Murakami ◽  
Norio Nakamura ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Near Infrared ◽  
Thoracoscopic Surgery ◽  
Good Method ◽  
Pleuroperitoneal Communication ◽  
Fluorescence Images ◽  
One Year

Abstract A pleuroperitoneal communication is a serious complication for patients undergoing continuous ambulatory peritoneal dialysis (CAPD). Video-assisted thoracoscopic surgery is performed using indocyanine green adsorbed to human serum albumin fluorescence to identify the communication because human serum albumin reinforces fluorescence images. A patient diagnosed with a pleuroperitoneal communication was referred to our department and underwent surgery. To detect the communication, a dialysate mixture that contained indocyanine green and human serum albumin was injected from the CAPD catheter. Real-time fluorescence images were able to clearly show a bleb-like lesion with a near-infrared spectroscopy camera, and the site was repaired. The patient had no recurrence at one-year follow-up. This method might be good method for pleuroperitoneal communication surgery.

Human serum albumin (HSA) coated liposomal indocyanine green for in vivo tumor imaging

RSC Advances ◽  
2016 ◽  
Vol 6 (18) ◽  
pp. 15220-15225 ◽  
Author(s):  
Siqin Chen ◽  
Gongjie Yu ◽  
Bo Zhang ◽  
Yinsong Wang ◽  
Ning Zhang ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Near Infrared ◽  
Tumor Imaging ◽  
Fluorescent Nanoprobe

In this study, a near-infrared (NIR) fluorescent nanoprobe based on indocyanine green (ICG) was synthesized.

A near infrared light-triggered human serum albumin drug delivery system with coordination bonding of indocyanine green and cisplatin for targeting photochemistry therapy against oral squamous cell cancer

2019 ◽  
Vol 7 (12) ◽  
pp. 5270-5282 ◽  
Author(s):  
Yuxin Wang ◽  
Diya Xie ◽  
Jiongru Pan ◽  
Chengwan Xia ◽  
Lei Fan ◽  
...  
Keyword(s):  
Drug Delivery ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Drug Delivery System ◽  
Delivery System ◽  
Near Infrared ◽  
Cell Cancer ◽  
Infrared Light

To ensure site–specific drug release in tumor cells and cancer-associated fibroblasts and reduce the systemic toxicity of chemotherapy, a novel drug delivery system called human serum albumin-indocyanine green-cisplatin nanoparticles was developed.

Smart Human Serum Albumin-Indocyanine Green Nanoparticles Generated by Programmed Assembly for Dual-Modal Imaging-Guided Cancer Synergistic Phototherapy

ACS Nano ◽  
2014 ◽  
Vol 8 (12) ◽  
pp. 12310-12322 ◽  
Author(s):  
Zonghai Sheng ◽  
Dehong Hu ◽  
Mingbin Zheng ◽  
Pengfei Zhao ◽  
Huilong Liu ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Programmed Assembly

Benefit of technetium-99mgalactosyl human serum albumin scintigraphy instead of indocyanine green test in patients scheduled for hepatectomy

2014 ◽  
Vol 44 (10) ◽  
pp. E118-E128 ◽  
Author(s):  
Yasuo Ohkura ◽  
Shugo Mizuno ◽  
Masashi Kishiwada ◽  
Takashi Hamada ◽  
Masanobu Usui ◽  
...  
Keyword(s):  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Indocyanine Green Test

scholarly journals Human Serum Albumin Decorated Indocyanine Green Improves Fluorescence-Guided Resection of Residual Lesions of Breast Cancer in Mice

2021 ◽  
Vol 11 ◽  
Author(s):  
Zun Wang ◽  
Min Chen ◽  
Jing-Jing Liu ◽  
Rong-He Chen ◽  
Qian Yu ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Human Serum Albumin ◽  
Serum Albumin ◽  
Indocyanine Green ◽  
Human Serum ◽  
Real Time ◽  
Mice Model ◽  
Cancer Tissues ◽  
Residual Lesions

ObjectiveAchieving negative resection margin is critical but challenging in breast-conserving surgery. Fluorescence-guided surgery allows the surgeon to visualize the tumor bed in real-time and to facilitate complete resection. We envisioned that intraoperative real-time fluorescence imaging with a human serum albumin decorated indocyanine green probe could enable complete surgical removal of breast cancer in a mouse model.MethodsWe prepared the probe by conjugating indocyanine green (ICG) with human serum albumin (HSA). In vitro uptake of the HSA-ICG probe was compared between human breast cancer cell line MDA-MB-231 and normal breast epithelial cell line MCF 10A. In vivo probe selectivity for tumors was examined in nude mice bearing MDA-MB-231-luc xenografts and the FVB/N-Tg (MMTV-PyMT) 634Mul/J mice model with spontaneous breast cancer. A positive-margin resection mice model bearing MDA-MB-231-luc xenograft was established and the performance of the probe in assisting surgical resection of residual lesions was examined.ResultsA significantly stronger fluorescence intensity was detected in MDA-MB-231 cells than MCF 10A cells incubated with HSA-ICG. In vivo fluorescence imaging showed that HSA-ICG had an obvious accumulation at tumor site at 24 h with tumor-to-normal tissue ratio of 8.19 ± 1.30. The same was true in the transgenic mice model. The fluorescence intensity of cancer tissues was higher than that of non-cancer tissues (58.53 ± 18.15 vs 32.88 ± 11.34). During the surgical scenarios, the residual tumors on the surgical bed were invisible with the naked eye, but were detected and resected with negative margin under HSA-ICG guidance in all the mice (8/8). Recurrence rate among mice that underwent resection with HSA-ICG (0/8) was significantly lower than the rates among mice with ICG (4/8), as well as the control group under white light (7/7).ConclusionsThis study suggests that real-time in vivo visualization of breast cancer with an HSA-ICG fluorescent probe facilitates complete surgical resection of breast cancer in a mouse xenograft model.

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