scholarly journals A Label-free Optical Sensing Platform for Beta-glucosidase Activity Using Protein-inorganic Hybrid Nanoflowers

2021 ◽  
Author(s):  
Ziping Liu ◽  
Shasha Liu ◽  
Decai Gao ◽  
Yanan Li ◽  
Ye Tian ◽  
...  
Keyword(s):  
Optical Sensing ◽  
Enzymatic Reaction ◽  
Label Free ◽  
Inorganic Hybrid ◽  
Sensing System ◽  
Amplex Red ◽  
Sensing Platform ◽  
Synthesis Strategy ◽  
Beta Glucosidase

Abstract In this work, a convenient, label-free and dual-signal readout optical sensing platform for the sensitively and selectively determination of beta-glucosidase (β-Glu) activity was reported using protein-inorganic hybrid nanoflowers [BSA-Cu3(PO4)2·3H2O] possessing peroxidase-mimicking activity. The nanoflowers (NFs) were facilely synthesized through a self-assembled synthesis strategy at room temperature. The as-prepared NFs could catalytically convert the colorless and non-fluorescent Amplex Red into colored and highly fluorescent resorufin in the presence of hydrogen peroxide via electron transfer process. β-Glu could hydrolyze cyanogenic glycoside, using amygdalin (Amy) as a model, into cyanide ions (CN-), which can subsequently efficiently suppress the catalytic activity of NFs, accompanied with the fluorescence decrease and the color fading. The concentration of CN- was controlled by β-Glu-triggered enzymatic reaction of Amy. Thus, a sensing system was established for fluorescent and visual determination of β-Glu activity. Under the optimum conditions, the present fluorescent and visual bimodal sensing platform exhibited good sensitivity for β-Glu activity assay with a detection limit of 0.33 U·L-1. The sensing platform was further applied to determinate β-Glu in real samples and satisfactory results were attained. Additionally, the label-free optical sensing system can potentially be a promising candidate for β-Glu inhibitors screening.

scholarly journals Label-Free Fluorescent Determination of Sunset Yellow in Soft Drinks Based on an Indicator-Displacement Assay

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Shilian Wu ◽  
Yanqiong Zhang ◽  
Long Yang ◽  
Can-Peng Li
Keyword(s):  
Epigallocatechin Gallate ◽  
Label Free ◽  
Fluorescence Sensing ◽  
Sunset Yellow ◽  
Sensing Platform ◽  
Potential Applications ◽  
Displacement Assay ◽  
Signal Probe ◽  
Target Molecules

This work reported a fluorescence sensing platform for Sunset Yellow (SY) determination based on competitive host-guest interaction between cucurbit[7]uril (CB7) and signal probe/target molecules. Luteolin/epigallocatechin gallate (EGCG) and SY were selected as the probe and target molecules, respectively. When luteolin or EGCG entered the CB7 host, its fluorescence significantly improved. However, upon the presence of SY in the performed luteolin·CB7 or EGCG·CB7 complex, this led to a remarkable decrease in fluorescence. This result was due to the fact that the binding constant of CB7/SY (4.9×104 M−1) was greater than that of CB7/luteolin (3.2×103 M−1) or CB7/EGCG (4.8×103 M−1). The fluorescence intensities of CB7/luteolin and CB7/EGCG complexes decreased linearly with increased SY concentration ranges of 0.5–50.0 and 2.0–50.0 μM. The proposed method had detection limits of 0.12 and 0.45 μM and was successfully used to determine SY samples with good recoveries ranging from 96.3% to 103.8%. This competitive mode provided a promising fluorescence assay strategy for potential applications in food safety.

Label-free and highly sensitive detection of DNA adenine methylation methyltransferase through cathodic photoelectrochemistry

The Analyst ◽  
2021 ◽  
Author(s):  
Fang Li ◽  
Xiu-Ming Wu ◽  
Mengmeng Gu ◽  
Guang-Li Wang
Keyword(s):  
Potassium Ferricyanide ◽  
Sensitive Detection ◽  
Label Free ◽  
Sensing System ◽  
Highly Sensitive ◽  
Adenine Methylation ◽  
Highly Sensitive Detection

In this work, we report the first exploration of cathodic photoelectrochemistry for the determination of the activity of DNA adenine methylation (Dam) methyltransferase (MTase). In this sensing system, potassium ferricyanide...

Affinity Control of Aminopropylsiloxane-based Organic/Inorganic Hybrids for Optical Sensors

2004 ◽  
Vol 847 ◽  
Author(s):  
Shingo Katayama ◽  
Noriko Yamada ◽  
Masanobu Awano
Keyword(s):  
Optical Sensors ◽  
Optical Sensing ◽  
Hybrid Structure ◽  
Inorganic Component ◽  
Amino Groups ◽  
Sensitive Material ◽  
Inorganic Hybrid ◽  
Sensing System ◽  
Sensing Systems ◽  
Hydrolysis Of

ABSTRACTThe aminopropylsiloxane-based organic/inorganic hybrid is known as a sensitive material toward NO2gas for optical sensing systems but it has the irreversible detection. The affinity of the aminopropylsiloxane-based organic/inorganic hybrid toward NO2gas was controlled by incorporating an Nb inorganic component derived from Nb(OC2H5)5into the hybrid structure. The amino groups had a strong coordination bond with hydrolyzed Nb(OC2H5)5and/or an acid-base pair with an oxoanion formed by hydrolysis of Nb(OC2H5)5. Thus, the strong solid-basicity of the aminopropylsiloxane-based organic/inorganic hybrid was weakened. The aminopropylsiloxane-based organic/inorganic hybrid containing an Nb inorganic component provided reversible sensing toward NO2gas in an optical sensing system. NO2is thought to be more weakly adsorbed because of the weakened basicity of the amino groups.

scholarly journals A label-free and sensitive photoluminescence sensing platform based on long persistent luminescence nanoparticles for the determination of antibiotics and 2,4,6-trinitrophenol

RSC Advances ◽  
2018 ◽  
Vol 8 (11) ◽  
pp. 5714-5720 ◽  
Author(s):  
Xi Zhang ◽  
Na-Yan Xu ◽  
Qiong Ruan ◽  
Dan-Qing Lu ◽  
Yun-Hui Yang ◽  
...  
Keyword(s):  
High Sensitivity ◽  
Persistent Luminescence ◽  
Label Free ◽  
Low Background ◽  
Background Interference ◽  
Sensing Platform ◽  
Highly Sensitive ◽  
Visualized Detection

A highly sensitive luminescence sensor based on PLNPs for visualized detection of antibiotic and organic explosives was developed, which could eliminate the background interference, allowing low background and therefore high sensitivity.

High-performance plasmonic lab-on-fiber sensing system constructed by universal polymer assisted transfer technique

2021 ◽  
Author(s):  
Guangrong Wang ◽  
Lei Wang ◽  
Zhan Cheng ◽  
Dan Chen ◽  
Xuemin Zhang ◽  
...  
Keyword(s):  
Optical Fibers ◽  
High Performance ◽  
Sacrificial Layer ◽  
Label Free ◽  
Plasmonic Sensors ◽  
Fiber Sensing ◽  
Refractive Index Sensing ◽  
Sensing System ◽  
Sensing Platform ◽  
Sensing Performance

Abstract Plasmonic lab-on-fiber (LOF) system has become an emerging sensing platform for the realization of miniaturized and portable plasmonic sensors. Herein, a facile and efficient polymer assisted transfer technique was reported for the preparation of plasmonic LOF systems. The proposed plasmonic LOF system was constructed through transferring plasmonic arrays to the end surface of optical fibers using polylactic acid (PLA) as the sacrificial layer. The morphology of the transferred plasmonic arrays maintains excellent consistency with the original arrays. Importantly, the as-prepared plasmonic LOF system also possesses outstanding sensing performance in refractive index sensing and quantitative label-free biosensing applications. Additionally, the proposed polymer assisted transfer technique shows broad universality for various plasmonic arrays. Together with the above features, it is believed that the polymer assisted transfer technique will show great potential for the application of future plasmonic LOF systems.

Portable glucose meter-utilized label-free and washing-free telomerase assay

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5578-5583
Author(s):  
Hyo Yong Kim ◽  
Chang Yeol Lee ◽  
Hansol Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Enzymatic Reaction ◽  
Telomerase Activity ◽  
Label Free ◽  
Glucose Meter

A portable glucose meter (PGM)-utilized method relying on kinase-catalyzed cascade enzymatic reaction (KCER) for the facile determination of telomerase activity was developed.

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