Portable glucose meter-utilized label-free and washing-free telomerase assay

The Analyst ◽  
2020 ◽  
Vol 145 (16) ◽  
pp. 5578-5583
Author(s):  
Hyo Yong Kim ◽  
Chang Yeol Lee ◽  
Hansol Kim ◽  
Ki Soo Park ◽  
Hyun Gyu Park
Keyword(s):  
Enzymatic Reaction ◽  
Telomerase Activity ◽  
Label Free ◽  
Glucose Meter

A portable glucose meter (PGM)-utilized method relying on kinase-catalyzed cascade enzymatic reaction (KCER) for the facile determination of telomerase activity was developed.

scholarly journals A Label-free Optical Sensing Platform for Beta-glucosidase Activity Using Protein-inorganic Hybrid Nanoflowers

2021 ◽  
Author(s):  
Ziping Liu ◽  
Shasha Liu ◽  
Decai Gao ◽  
Yanan Li ◽  
Ye Tian ◽  
...  
Keyword(s):  
Optical Sensing ◽  
Enzymatic Reaction ◽  
Label Free ◽  
Inorganic Hybrid ◽  
Sensing System ◽  
Amplex Red ◽  
Sensing Platform ◽  
Synthesis Strategy ◽  
Beta Glucosidase

Abstract In this work, a convenient, label-free and dual-signal readout optical sensing platform for the sensitively and selectively determination of beta-glucosidase (β-Glu) activity was reported using protein-inorganic hybrid nanoflowers [BSA-Cu3(PO4)2·3H2O] possessing peroxidase-mimicking activity. The nanoflowers (NFs) were facilely synthesized through a self-assembled synthesis strategy at room temperature. The as-prepared NFs could catalytically convert the colorless and non-fluorescent Amplex Red into colored and highly fluorescent resorufin in the presence of hydrogen peroxide via electron transfer process. β-Glu could hydrolyze cyanogenic glycoside, using amygdalin (Amy) as a model, into cyanide ions (CN-), which can subsequently efficiently suppress the catalytic activity of NFs, accompanied with the fluorescence decrease and the color fading. The concentration of CN- was controlled by β-Glu-triggered enzymatic reaction of Amy. Thus, a sensing system was established for fluorescent and visual determination of β-Glu activity. Under the optimum conditions, the present fluorescent and visual bimodal sensing platform exhibited good sensitivity for β-Glu activity assay with a detection limit of 0.33 U·L-1. The sensing platform was further applied to determinate β-Glu in real samples and satisfactory results were attained. Additionally, the label-free optical sensing system can potentially be a promising candidate for β-Glu inhibitors screening.

Enzymatic colorimetry of lecithin and sphingomyelin in aqueous solution.

1983 ◽  
Vol 29 (8) ◽  
pp. 1513-1517 ◽  
Author(s):  
M W McGowan ◽  
J D Artiss ◽  
B Zak
Keyword(s):  
Aqueous Solution ◽  
Hydrogen Peroxide ◽  
Amniotic Fluid ◽  
Enzymatic Reaction ◽  
Detergent Solution ◽  
Enzymatic Determination ◽  
Red Color ◽  
Hydrolysis Of ◽  
Zwitterionic Detergent

Abstract A procedure for the enzymatic determination of lecithin and sphingomyelin in aqueous solution is described. The phospholipids are first dissolved in chloroform:methanol (2:1 by vol), the solvent is evaporated, and the residue is redissolved in an aqueous zwitterionic detergent solution. The enzymatic reaction sequences of both assays involve hydrolysis of the phospholipids to produce choline, which is then oxidized to betaine, thus generating hydrogen peroxide. The hydrogen peroxide is subsequently utilized in the enzymatic coupling of 4-aminoantipyrine and sodium 2-hydroxy-3,5-dichlorobenzenesulfonate, an intensely red color being formed. The presence of a non-reacting phospholipid enhances the hydrolysis of the reacting phospholipid. Thus we added lecithin to the sphingomyelin standards and sphingomyelin to the lecithin standards. This precise procedure may be applicable to determination of lecithin and sphingomyelin in amniotic fluid.

Label-Free Detection of Telomerase Activity in Urine Using Telomerase-Responsive Porous Anodic Alumina Nanochannels

2016 ◽  
Vol 88 (16) ◽  
pp. 8107-8114 ◽  
Author(s):  
Xu Liu ◽  
Min Wei ◽  
Yuanjian Liu ◽  
Bingjing Lv ◽  
Wei Wei ◽  
...  
Keyword(s):  
Telomerase Activity ◽  
Anodic Alumina ◽  
Porous Anodic Alumina ◽  
Label Free ◽  
Label Free Detection

Performance of the Ascensia ENTRUST glucose meter for determination of blood glucose concentration in rats

2009 ◽  
Vol 38 (4) ◽  
pp. 493-500
Author(s):  
S. Vijayakumar ◽  
Nancy E. Everds ◽  
P. Kalaiselvan ◽  
R.K. Shakthidevan ◽  
Yogesh Kumar V. Murkunde ◽  
...  
Keyword(s):  
Blood Glucose ◽  
Glucose Concentration ◽  
Blood Glucose Concentration ◽  
Glucose Meter

Label-Free Determination of the Cell Cycle Phase in Human Embryonic Stem Cells by Raman Microspectroscopy

2013 ◽  
Vol 85 (19) ◽  
pp. 8996-9002 ◽  
Author(s):  
Stanislav O. Konorov ◽  
H. Georg Schulze ◽  
James M. Piret ◽  
Michael W. Blades ◽  
Robin F. B. Turner
Keyword(s):  
Cell Cycle ◽  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryonic Stem Cells ◽  
Embryonic Stem ◽  
Raman Microspectroscopy ◽  
Cell Cycle Phase ◽  
Cycle Phase ◽  
Label Free

Label-free RF biosensors for human cell dielectric spectroscopy

2009 ◽  
Vol 1 (6) ◽  
pp. 497-504 ◽  
Author(s):  
Claire Dalmay ◽  
Arnaud Pothier ◽  
Mathilde Cheray ◽  
Fabrice Lalloue ◽  
Marie-Odile Jauberteau ◽  
...  
Keyword(s):  
Human Cell ◽  
Cell Types ◽  
Label Free ◽  
Microwave Frequencies ◽  
Non Invasive ◽  
System Cell ◽  
Electromagnetic Characteristics ◽  
Biosensor Chip ◽  
Rf Signal

This paper presents an original biosensor chip allowing determination of intrinsic relative permittivity of biological cells at microwave frequencies. This sensor permits non-invasive cell identification and discrimination using an RF signal to probe intracellular medium of biological samples. Indeed, these sensors use an RF planar resonator that allows detection capabilities on less than 10 cells, thanks to the microscopic size of its sensitive area. Especially, measurements between 15 and 35 GHz show the ability label-free biosensors to differentiate two human cell types using their own electromagnetic characteristics. The real part of permittivity of cells changes from 20 to 48 for the nervous system cell types studied. The proposed biodetection method is detailed and we show how the accuracy and the repeatability of measurements have been improved to reach reproducible measurements.

Label Free Determination of Potassium Ions Using Crystal Violet and Thrombin-Binding Aptamer

2014 ◽  
Vol 47 (10) ◽  
pp. 1726-1736 ◽  
Author(s):  
Bin Zheng ◽  
Sheng Cheng ◽  
Huaze Dong ◽  
Haojun Liang ◽  
Jian Liu ◽  
...  
Keyword(s):  
Crystal Violet ◽  
Potassium Ions ◽  
Label Free
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