scholarly journals The Effect of Alcoholic Extract of Thymus Vulgaris on Hepatic Enzymes Activity and Apoptosis-Related Gene Expression in Streptozotocin-Induced Diabetic Rats

2021 ◽  
Author(s):  
Mohammadreza Azimi ◽  
Elnaz Ahmadi ◽  
Fatemeh Aghaie ◽  
Mohammad Orafei ◽  
Armita Ahmadi ◽  
...  
Keyword(s):  
Gene Expression ◽  
Liver Enzymes ◽  
Diabetic Rats ◽  
Thymus Vulgaris ◽  
Alcoholic Extract ◽  
Diabetic Mice ◽  
Related Gene ◽  
Hepatic Enzymes ◽  
Enzymes Activity ◽  
Apoptosis Related Gene

Abstract Many diseases, including diabetes, are involve in the development of liver disorders through changes in the expression of genes such as apoptosis-related gene. In the present study, the effect of alcoholic extract of Thymus Vulgaris on Hepatic Enzymes Activity and apoptosis-related gene expression in streptozotocin-induced diabetic rats. In this study, 50 adult male Wistar rats weighing approximately 200–220 g were divided into five groups. Diabetes was induced by intraperitoneal injection of STZ (60 mg/kg). Following 18 days, all the animals in different groups were weighed and blood samples were taken from their cardiac veins. GC analysis revealed 45 different compounds in the Thymus Vulgaris, including; thymol (39.1%), p-cymene (20.63%), γ-Terpinene (14.85%). The results showed a significant increase in liver enzymes (AST, ALT and ALP) in diabetic or streptozoic mice compared to the control group (healthy mice) (p < 0.0001). The level of liver enzymes (AST, ALT and ALP) in rats treated with doses 200 mg/kg and 400 mg/kg of thymus vulgaris extract showed a significant decrease in these enzymes in comparison with diabetic rats (p < 0.0001). The expression of caspase 3 and 9 genes in the groups treated with thyme significantly decreased compared to diabetic mice (P < 0.0001) and the expression of Bcl in the group receiving 400 mg / kg of thyme significantly increased compared to diabetic mice (P = 0.0001). Due to its antioxidant compounds, thyme improves the liver tissue cells in streptozotocin-induced diabetic mice by reducing caspases 3 and 9 as well as increasing Bcl-2.

The effects of grape seed on apoptosis-related gene expression and oxidative stress in streptozotocin-induced diabetic rats

Renal Failure ◽  
2015 ◽  
Vol 37 (2) ◽  
pp. 192-197 ◽  
Author(s):  
Aydın Güçlü ◽  
Nilüfer Yonguç ◽  
Yavuz Dodurga ◽  
Gülşah Gündoğdu ◽  
Zuhal Güçlü ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Expression ◽  
Grape Seed ◽  
Diabetic Rats ◽  
Related Gene ◽  
And Oxidative Stress ◽  
Apoptosis Related Gene

scholarly journals Apoptosis-related gene expression can predict the response of ovarian cancer cell lines to treatment with recombinant human TRAIL alone or combined with cisplatin

Clinics ◽  
2020 ◽  
Vol 75 ◽  
Author(s):  
Letícia da Conceição Braga ◽  
Nikole Gontijo Gonçales ◽  
Rafaela de Souza Furtado ◽  
Warne Pedro de Andrade ◽  
Luciana Maria Silva ◽  
...  
Keyword(s):  
Gene Expression ◽  
Ovarian Cancer ◽  
Cell Lines ◽  
Cancer Cell ◽  
Ovarian Cancer Cell ◽  
Cancer Cell Lines ◽  
Related Gene ◽  
Ovarian Cancer Cell Lines ◽  
Apoptosis Related Gene

Enhancement of Apoptosis-Related Gene Expression in Human Renal Graft Rejection

2000 ◽  
Vol 6 (S2) ◽  
pp. 626-627
Author(s):  
P. Y. Lau ◽  
J. Papadimitriou ◽  
C. Drachenberg ◽  
M. R. Weir ◽  
C. Wei
Keyword(s):  
Gene Expression ◽  
Kidney Transplantation ◽  
Graft Rejection ◽  
Human Kidney ◽  
Renal Tissue ◽  
Related Gene ◽  
Renal Graft ◽  
Normal Human ◽  
Apoptosis Related Gene ◽  
Renal Graft Rejection

Apoptosis or programmed cell death is involved in many diseases include end-stage renal failure. Apoptosis-related genes include both stimulate genes and inhibitory gene of apoptosis. The genes which stimulate apoptosis include p53 and p21-WAF. The genes which inhibit apoptosis include bcl-2 gene family. The mechanisms of apoptosis include p53-dependend pathway and p53- independent pathway. We hypothesized that apoptosis-related genes may activate in renal graft rejection after kidney transplantation. Therefore, the present study was designed to investigate apoptosis-related gene expression and localization by immunohistochemical staining (IHCS) in human renal tissues with graft rejection and compare with that in normal human renal tissue.Human renal biopsy (n=5) were obtained after kidney transplantation with mild and moderate renal rejection. Normal human kidney biopsy was obtained during nephrectomy. P53, p21-WAF and Bcl-2 levels in renal tissue were determined by IHCS. The results of IHCS was evaluated by IHCS staining density scores (0, no staining; 1, minimal staining; 2, mild staining; 3, moderate staining; and 4, strong staining).

scholarly journals Apoptosis-Related Gene Expression Profiling in Hematopoietic Cell Fractions of MDS Patients

PLoS ONE ◽  
2016 ◽  
Vol 11 (11) ◽  
pp. e0165582 ◽  
Author(s):  
Saskia MC Langemeijer ◽  
Niccolo Mariani ◽  
Ruth Knops ◽  
Christian Gilissen ◽  
Rob Woestenenk ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Hematopoietic Cell ◽  
Related Gene ◽  
Cell Fractions ◽  
Apoptosis Related Gene

Proliferation and Apoptosis-Related Gene Expression in Experimental Acquired Immunodeficiency Syndrome-Related Simian Lymphoma

Blood ◽  
1999 ◽  
Vol 93 (4) ◽  
pp. 1364-1371
Author(s):  
Esmeralda Castaños-Vélez ◽  
Thomas Heiden ◽  
Marianne Ekman ◽  
Joseph Lawrence ◽  
Gunnel Biberfeld ◽  
...  
Keyword(s):  
Gene Expression ◽  
Acquired Immunodeficiency Syndrome ◽  
Proliferative Activity ◽  
Epstein Barr Virus ◽  
Related Gene ◽  
Barr Virus ◽  
Acquired Immunodeficiency ◽  
Epstein Barr ◽  
Immunodeficiency Syndrome ◽  
Apoptosis Related Gene

Lymphomas in 10 cynomolgus monkeys infected with a simian immunodeficiency virus (SIVsm) were studied with regard to proliferative activity and apoptosis-related gene expression. All were diffuse large-cell lymphomas, showed mono or oligoclonality and a 9/10 diploid cellular DNA content. Expression of a simian homologue to Epstein-Barr virus (HVMF-1) was shown in nine cases. The lymphomas showed moderate to high proliferative activity by Ki67 immunostaining and DNA flow cytometry, and a low number of apoptotic cells detected by TdT-mediated dUTP nick-end labeling (TUNEL). Immunohistochemistry showed abundant tumor infiltrating TIA-1+ cytotoxic lymphocytes (CTL) and macrophages. Bcl-2, Mcl-1, and also Bax and Bak, but not p53 were demonstrable in the tumor cells by immunostaining. Our findings suggest a causal relationship between HVMF-1 infection and a low apoptotic index of the lymphomas due to the expression of Bcl-2. The apparent inefficient function of tumor-infiltrating CTL could be due to inactivation of CTL and/or resistance of the lymphoma cells to CTL effects. The tumors showed immunoreactivity for CD18, CD29, and CD49d, but not for CD11a, mimicking the phenotype of human Epstein-Barr virus (EBV)–related lymphomas. In summary, our observations indicate a high similarity between this simian model of acquired immunodeficiency syndrome (AIDS)-related lymphomas (ARL) and human ARL and other immunosuppression-related lymphomas.

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