scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test. Results The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2020 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis.Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis.Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF Levels in the Crevicular Fluid of Patients With Periodontitis or Healthy Implants

2020 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Clinical Manifestations ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Appropriate Therapy ◽  
Crevicular Fluid ◽  
Therapy Intervention

Abstract Purpose: The evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data implies that an examination of cytokine level in peri-implant sulcular fluid (PISF) might prove useful when evaluating the condition of peri-implant tissues and monitoring the development of peri-implant inflammation, including both mucositis and peri-implantitis. Thus, in this paper, it has been decided to assess the level of TNF, CXCL8, and IL-1β in PISF collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Materials and Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for TNF, CXCL8, and IL-1β levels using an ELISA test.Results: We documented that the IL-1β level, in PISF in patients with implants, was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. We also revealed that their CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. However, the TNF level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Our observation might imply that the monitoring of TNF, CXCL8, and IL-1β levels in PISF could help with the diagnosis of mucositis/peri-implantitis before any clinical manifestations, thus allowing a quicker appropriate therapy intervention at an early stage.

scholarly journals Evaluation of Metalloproteinase-8 Levels in Crevicular Fluid of Patients with Healthy Implants or Periodontitis

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Paulina Żelechowska ◽  
Justyna Agier ◽  
Katarzyna Starska ◽  
Krzysztof Kędzierski ◽  
...  
Keyword(s):  
Clinical Examination ◽  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Clinical Manifestations ◽  
Elisa Test ◽  
Imaging Diagnostics ◽  
Periodontal Inflammation ◽  
Appropriate Therapy ◽  
Crevicular Fluid

Evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data imply that Metalloproteinase-8 (MMP-8) level examination in peri-implant sulcular fluid (PISF) might be useful for evaluating the condition of peri-implant tissues and monitoring a development of peri-implant inflammation, including both mucositis and peri-implantitis. Hence, in this study, we decided to evaluate the level of MMP-8 in PISF obtained from patients without clinical symptoms of mucositis or peri-implantitis and compare it with MMP-8 level in gingival crevicular fluid (GCF) obtained from patients with healthy periodontium and those with varying severity of periodontitis. A total of 189 subjects were included in the study, and GCF/PISF samples were analysed for MMP-8 level by ELISA test. We documented that MMP-8 level in PISF obtained from patients without symptoms of mucositis or peri-implantitis was significantly higher not only than in GCF of periodontally healthy patients but also, which seems to be very interesting, than in GCF of patients with varying degrees of periodontal inflammation, consistent with earlier studies. Our observation might imply that monitoring of MMP-8 level in PISF could help to diagnose mucositis/peri-implantitis in an early stage, prior to clinical manifestations, which may allow for quick start of appropriate therapy.

Comparative Evaluation of Cytokines in Gingival Crevicular Fluid and Saliva of Patients with Aggressive Periodontitis

2013 ◽  
Vol 28 (1) ◽  
pp. 108-112 ◽  
Author(s):  
Yuan Yue ◽  
Qing Liu ◽  
Chunjiao Xu ◽  
Wings TY Loo ◽  
Min Wang ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Aggressive Periodontitis ◽  
Control Group ◽  
Clinical Parameters ◽  
Quick Method ◽  
Probing Depth ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Before And After ◽  
Crevicular Fluid

Objectives This study aims to evaluate and compare cytokines in gingival crevicular fluid (GCF) and saliva of patients with aggressive periodontitis (AP) before and after treatment. Methods Forty AP patients and 40 healthy volunteers were enrolled in this study. Clinical parameters included probing depth and sulcus bleeding index. GCF and saliva were collected from both groups. The levels of IL-1β, IL-2, IL-4, IL-6, IFN-γ and TNF-α were measured using ELISA. Results The probing depth in AP patients was significantly deeper before treatment than after treatment. The concentrations of cytokines in GCF and saliva were significantly higher in AP patients than in the control group and decreased after periodontal treatment. Positive relationships were found between cytokine levels in GCF and clinical parameters. The reliability of cytokines in GCF and saliva was assessed by Cronbach's alpha analysis, which could be considered satisfactory. Conclusion Cytokine levels in GCF and saliva correlated well with clinical parameters and AP. Measurements of cytokines in saliva may be regarded as a noninvasive and quick method for monitoring periodontal disease activity.

scholarly journals Two mixed-ligand coordination polymers based on 2,5-thiophenedicarboxylic acid and flexible N-donor ligands: the protective effect on periodontitis via reducing the release of IL-1β and TNF-α

2020 ◽  
Vol 18 (1) ◽  
pp. 391-398
Author(s):  
Shao-Hsuan Wu ◽  
Jun-Hui Huang
Keyword(s):  
Coordination Polymers ◽  
Gingival Crevicular Fluid ◽  
Mixed Ligand ◽  
Donor Ligands ◽  
X Ray Diffraction ◽  
Functional Studies ◽  
Tnf Α ◽  
Mucosal Cells ◽  
Ligand Coordination ◽  
Crevicular Fluid

AbstractTwo novel mixed-ligand coordination polymers, {[Co(tdc)(btrp)]·0.67DMF}n (1) and {[Zn2(bimb)2(tdc)2]·2H2O}n (2) involving 2,5-thiophenedicarboxylate (H2tdc), and bitopic flexible N-donor ligands, 1,3-bis(1,2,4-triazol-1-yl)propane (btrp) and 1,4-bis((1H-benzo[d]imidazol-1-yl)methyl)benzene (bimb), have been synthesized by the hydrothermal method and characterized via IR, elemental analysis, thermal analysis, and powder X-ray diffraction. The biological functional studies were performed; the treatment activity of the compounds on periodontitis and the specific mechanism was explored. First, the real-time RT-PCR was carried out to determine the inflammatory genes nf-κb and p53 relative expression in periodontal mucosal cells after treating with compounds 1 and 2. Then, the level of the inflammatory cytokine in the gingival crevicular fluid after treating with compounds was also determined by the ELISA detection kit.

scholarly journals Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis

2019 ◽  
Vol 8 (5) ◽  
pp. 630 ◽  
Author(s):  
Kaja Eriksson ◽  
Guozhong Fei ◽  
Anna Lundmark ◽  
Daniel Benchimol ◽  
Linkiat Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Mediators ◽  
Gingival Crevicular Fluid ◽  
Severe Form ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Periodontal Health ◽  
Microbial Profile ◽  
Severe Periodontitis ◽  
Crevicular Fluid

This study aimed to investigate the periodontal health of patients with established rheumatoid arthritis (RA) in relation to oral microbiota, systemic and oral inflammatory mediators, and RA disease activity. Forty patients underwent full-mouth dental/periodontal and rheumatological examination, including collection of blood, saliva, gingival crevicular fluid (GCF) and subgingival plaque. Composition of plaque and saliva microbiota were analysed using 16S rRNA sequencing and levels of inflammatory mediators by multiplex-immunoassay. The majority of the patients (75%) had moderate or severe periodontitis and the rest had no/mild periodontitis. Anti-citrullinated protein antibody (ACPA) positivity was significantly more frequent in the moderate/severe periodontitis (86%) compared to the no/mild group (50%). No significance between groups was observed for RA disease duration or activity, or type of medication. Levels of sCD30/TNFRSF8, IFN-α2, IL-19, IL-26, MMP-1, gp130/sIL-6Rß, and sTNF-R1 were significantly higher in serum or GCF, and April/TNFSF13 was significantly higher in serum and saliva samples in moderate/severe periodontitis. The microbial composition in plaque also differed significantly between the two groups. In conclusion, the majority of RA patients had moderate/severe periodontitis and that this severe form of the disease was significantly associated with ACPA positivity, an altered subgingival microbial profile, and increased levels of systemic and oral inflammatory mediators.

scholarly journals Cytokines in crevicular fluid and orthodontic force: a systematic review

2017 ◽  
Vol 32 (2) ◽  
pp. 104 ◽  
Author(s):  
Susiane Allgayer ◽  
Luciane Macedo De Menezes ◽  
Mariana Roennau Lemos Rinaldi
Keyword(s):  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Cellular Level ◽  
Reference Tracking ◽  
Mesh Terms ◽  
Cytokine Levels ◽  
Synergistic Regulation ◽  
Handling Methods ◽  
Crevicular Fluid

OBJECTIVE: This paper aimed to analyze studies in the literature addressing the role of cytokines in the gingival crevicular fluid (GCF) in the orthodontic treatment.METHODS: Several databases were surveyed using both MESH terms and free terms. Additional studies were obtained by reference tracking. This review was registered in PROSPERO, and the procedures foreseen by its statement were followed. Data were obtained from the included studies addressing the orthodontic mechanics, GCF sampling/handling methods, and cytokine measurements. Clinical studies written in English were browsed. Papers were selected by one reviewer and checked by a second investigator.RESULTS: A total of 115 articles were identified, among which 25 were selected for detailed analysis. Common drawbacks consisted mainly of inadequacies in the study design (e.g. short duration and small number of study subjects). The most consistent result was a peak of cytokine levels at 1 d. Associations were observed between prostaglandin E2 (PGE2) and interleukin-1β (IL-1β) and pain, velocity of tooth movement, and treatment mechanics. Interleukin-1b and PGE2 showed different patterns of up-regulation, with IL-1β being more responsive to mechanical stress and PGE2 more responsive to synergistic regulation of IL-1β and mechanical force. The results support the use of light continuous forces for orthodontic treatment.CONCLUSION: There was a tendency of maintenance of relatively high IL-1β levels for longer periods with the use of light continuous forces, which might decrease the frequency of activation. These outcomes provide evidence at the cellular level for the utilization of light continuous forces.

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