scholarly journals Analysis of IL-1β, CXCL8, and TNF Levels in the Crevicular Fluid of Patients With Periodontitis or Healthy Implants

2020 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Clinical Manifestations ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Appropriate Therapy ◽  
Crevicular Fluid ◽  
Therapy Intervention

Abstract Purpose: The evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data implies that an examination of cytokine level in peri-implant sulcular fluid (PISF) might prove useful when evaluating the condition of peri-implant tissues and monitoring the development of peri-implant inflammation, including both mucositis and peri-implantitis. Thus, in this paper, it has been decided to assess the level of TNF, CXCL8, and IL-1β in PISF collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Materials and Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for TNF, CXCL8, and IL-1β levels using an ELISA test.Results: We documented that the IL-1β level, in PISF in patients with implants, was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. We also revealed that their CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. However, the TNF level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Our observation might imply that the monitoring of TNF, CXCL8, and IL-1β levels in PISF could help with the diagnosis of mucositis/peri-implantitis before any clinical manifestations, thus allowing a quicker appropriate therapy intervention at an early stage.

scholarly journals Evaluation of Metalloproteinase-8 Levels in Crevicular Fluid of Patients with Healthy Implants or Periodontitis

2017 ◽  
Vol 2017 ◽  
pp. 1-7 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Paulina Żelechowska ◽  
Justyna Agier ◽  
Katarzyna Starska ◽  
Krzysztof Kędzierski ◽  
...  
Keyword(s):  
Clinical Examination ◽  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Clinical Manifestations ◽  
Elisa Test ◽  
Imaging Diagnostics ◽  
Periodontal Inflammation ◽  
Appropriate Therapy ◽  
Crevicular Fluid

Evaluation of periodontal and peri-implant tissue condition is mainly based on clinical examination and imaging diagnostics. Some data imply that Metalloproteinase-8 (MMP-8) level examination in peri-implant sulcular fluid (PISF) might be useful for evaluating the condition of peri-implant tissues and monitoring a development of peri-implant inflammation, including both mucositis and peri-implantitis. Hence, in this study, we decided to evaluate the level of MMP-8 in PISF obtained from patients without clinical symptoms of mucositis or peri-implantitis and compare it with MMP-8 level in gingival crevicular fluid (GCF) obtained from patients with healthy periodontium and those with varying severity of periodontitis. A total of 189 subjects were included in the study, and GCF/PISF samples were analysed for MMP-8 level by ELISA test. We documented that MMP-8 level in PISF obtained from patients without symptoms of mucositis or peri-implantitis was significantly higher not only than in GCF of periodontally healthy patients but also, which seems to be very interesting, than in GCF of patients with varying degrees of periodontal inflammation, consistent with earlier studies. Our observation might imply that monitoring of MMP-8 level in PISF could help to diagnose mucositis/peri-implantitis in an early stage, prior to clinical manifestations, which may allow for quick start of appropriate therapy.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Vol 21 (1) ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test. Results The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2021 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis. Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis. Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Analysis of IL-1β, CXCL8, and TNF-α levels in the crevicular fluid of patients with periodontitis or healthy implants

2020 ◽  
Author(s):  
Paweł Aleksandrowicz ◽  
Ewa Brzezińska-Błaszczyk ◽  
Elżbieta Kozłowska ◽  
Paulina Żelechowska ◽  
Andrea Enrico Borgonovo ◽  
...  
Keyword(s):  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Elisa Test ◽  
Cytokine Concentration ◽  
Severe Periodontitis ◽  
Tnf Α ◽  
Cytokine Levels ◽  
Risk Patients ◽  
Α Level ◽  
Crevicular Fluid

Abstract Background: Our study aimed to assess the level of IL-1β, CXCL8, and TNF-α in peri-implant sulcular fluid (PISF) collected from patients with no clinical symptoms of mucositis or peri-implantitis and compare them with cytokine concentration in gingival crevicular fluid (GCF) acquired from patients with healthy periodontium and those with varying severity of periodontitis.Methods: A total of 189 subjects were included in the study, and GCF/PISF samples were checked for IL-1β, CXCL8, and TNF-α levels using an ELISA test.Results: The IL-1β level in PISF in patients with implants was significantly lower than in GCF in patients with mild, moderate, or severe periodontitis. The CXCL8 level in PISF was considerably lower than in patients with moderate periodontitis. The TNF-α level in PISF in patients with implants was markedly higher compared to subjects with healthy periodontium or patients with mild periodontitis.Conclusion: Analysis of cytokine levels may help describe the pathogenesis and early diagnosis of peri-implantitis and prevision in high-risk patients.

scholarly journals Evaluation of IL1β and IL6 Gingival Crevicular Fluid Levels during the Early Phase of Orthodontic Tooth Movement in Adolescents and Young Adults

2021 ◽  
Vol 11 (2) ◽  
pp. 521
Author(s):  
Simina Chelărescu ◽  
Petra Șurlin ◽  
Mioara Decusară ◽  
Mădălina Oprică ◽  
Eugen Bud ◽  
...  
Keyword(s):  
Young Adults ◽  
Orthodontic Treatment ◽  
Tooth Movement ◽  
Gingival Crevicular Fluid ◽  
Early Stage ◽  
Statistical Tests ◽  
Orthodontic Tooth Movement ◽  
Adolescents And Young Adults ◽  
Periodontal Tissues ◽  
Crevicular Fluid

Background: The crevicular fluid analysis represents a useful diagnosis tool, with the help of which noninvasive cellular metabolic activity can be analyzed. The aim of the study is to investigate comparatively IL1β and IL6 in the gingival crevicular fluid of clinically healthy adolescents and young adults during the acute phase of orthodontic treatment. Methods: Gingival crevicular fluid was collected from 20 patients (aged between 11 and 28) undergoing orthodontic treatment. Measurements were taken before (T0) and after 24 h after distalization forces were activated (T1). IL1β and IL 6 were analyzed using Elisa tests. The statistical tests used were two-sided t tests. Results: Between the two time periods there was a significant raise both in the crevicular fluid rate (0.57 µL at T0 vs. 0.95 µL at T1, p = 0.001) and in IL1β levels (15.67 pg/µL at T0 vs. 27.94 pg/µL at T1, p = 0.009). We were able to identify IL6 only in a third of the sites. There is a significantly increased level of ILβ at T1 in adolescents, more than in young adults (42.96 pg/µL vs. 17.93 pg/µL, p = 0.006). Conclusions: In the early stage of orthodontic treatment, the periodontal tissues of adolescents are more responsive to orthodontic forces than those of young adults.

Marfan Syndrome

2005 ◽  
Vol 44 (04) ◽  
pp. 487-497 ◽  
Author(s):  
G. Mátyás ◽  
B. Steinmann ◽  
D. Baumgartner ◽  
C. Baumgartner
Keyword(s):  
Medical Treatment ◽  
Marfan Syndrome ◽  
Clinical Symptoms ◽  
Early Stage ◽  
Molecular Genetic ◽  
Clinical Manifestations ◽  
Hierarchical Cluster ◽  
Molecular Genetic Analysis ◽  
Missense Mutations ◽  
Surgical Interventions

Summary Objectives: Marfan syndrome (MFS) is an autosomal dominant inherited connective tissue disorder caused by mutations in the fibrillin-1 (FBN1) gene with variable clinical manifestations in the cardiovascular, musculoskeletal and ocular systems. Methods: Data of molecular genetic analysis and a catalogue of clinical manifestations including aortic elastic parameters were mined in order to (i) assess aortic abnormality before and during medical treatment, and to (ii) identify novel correlations between the genotype and phenotype of the disease using hierarchical cluster analysis and logistic regression analysis. A score measure describing the similarity between a patient’s clinical symptoms and a characteristic phenotype class was introduced. Results: A probabilistic model for monitoring the loss of aortic elasticity was built on merely aortic parameters of 34 patients with classic MFS and 43 control subjects showing a sensitivity of 82% and a specificity of 96%. The clinical phenotypes of 100 individuals with classical or suspected MFS were clustered yielding four different phenotypic expressions. The highest correlation was found between FBN1 missense mutations, which manifested as ectopia lentis, skeletal major and skin minor criteria, and two out of four clustered phenotypes. The probability of the presence of a missense mutation in both phenotype classes is approximately 70%. Conclusions: Monitoring of aortic elastic properties during medical treatment may serve as additional criterion to indicate elective surgical interventions. Genotype-phenotype correlation may contribute to anticipate the clinical consequences of specific FBN1 mutations more comprehensively and may be helpful to identify MFS patients at risk at an early stage of disease.

scholarly journals Periodontal Health and Oral Microbiota in Patients with Rheumatoid Arthritis

2019 ◽  
Vol 8 (5) ◽  
pp. 630 ◽  
Author(s):  
Kaja Eriksson ◽  
Guozhong Fei ◽  
Anna Lundmark ◽  
Daniel Benchimol ◽  
Linkiat Lee ◽  
...  
Keyword(s):  
Rheumatoid Arthritis ◽  
Inflammatory Mediators ◽  
Gingival Crevicular Fluid ◽  
Severe Form ◽  
Oral Microbiota ◽  
Microbial Composition ◽  
Periodontal Health ◽  
Microbial Profile ◽  
Severe Periodontitis ◽  
Crevicular Fluid

This study aimed to investigate the periodontal health of patients with established rheumatoid arthritis (RA) in relation to oral microbiota, systemic and oral inflammatory mediators, and RA disease activity. Forty patients underwent full-mouth dental/periodontal and rheumatological examination, including collection of blood, saliva, gingival crevicular fluid (GCF) and subgingival plaque. Composition of plaque and saliva microbiota were analysed using 16S rRNA sequencing and levels of inflammatory mediators by multiplex-immunoassay. The majority of the patients (75%) had moderate or severe periodontitis and the rest had no/mild periodontitis. Anti-citrullinated protein antibody (ACPA) positivity was significantly more frequent in the moderate/severe periodontitis (86%) compared to the no/mild group (50%). No significance between groups was observed for RA disease duration or activity, or type of medication. Levels of sCD30/TNFRSF8, IFN-α2, IL-19, IL-26, MMP-1, gp130/sIL-6Rß, and sTNF-R1 were significantly higher in serum or GCF, and April/TNFSF13 was significantly higher in serum and saliva samples in moderate/severe periodontitis. The microbial composition in plaque also differed significantly between the two groups. In conclusion, the majority of RA patients had moderate/severe periodontitis and that this severe form of the disease was significantly associated with ACPA positivity, an altered subgingival microbial profile, and increased levels of systemic and oral inflammatory mediators.

scholarly journals Effects of Standardised Fermented Papaya Gel on Clinical Symptoms, Inflammatory Cytokines, and Nitric Oxide Metabolites in Patients with Chronic Periodontitis: An Open Randomised Clinical Study

2016 ◽  
Vol 2016 ◽  
pp. 1-12 ◽  
Author(s):  
Zaira F. Kharaeva ◽  
Lyana R. Zhanimova ◽  
Magomet Sh. Mustafaev ◽  
Chiara De Luca ◽  
Wolfgang Mayer ◽  
...  
Keyword(s):  
Nitric Oxide ◽  
Gingival Crevicular Fluid ◽  
Clinical Symptoms ◽  
Topical Administration ◽  
Control Group ◽  
Pocket Depth ◽  
Bacterial Killing ◽  
Anti Inflammatory ◽  
Nitric Oxide Metabolites ◽  
Crevicular Fluid

The clinical efficacy of topical administration of standardised fermented papaya gel (SFPG), known to have antioxidant and anti-inflammatory properties,versusconventional therapy was evaluated in a group of 84 patients with moderate-to-severe periodontitis, randomly assigned to control group (n=45) undergoing traditional pharmacologic/surgical protocols or to experimental group (n=39), additionally treated with intragingival pocket SFPG (7 g) applications (15 min daily for 10 days). Patients undergoing SFPG treatment showed significant (P<0.05), durable improvement of three major clinical indices of disease severity: reduced bleeding (day 7), plaque and gingival conditions (day 14), and consistent gingival pocket depth reduction (day 45). Proinflammatory nitric oxide metabolites reached normal values in plasma (day 14) and gingival crevicular fluid (GCF) at day 45 with SFPG applications compared to controls that did not reach normalisation. Levels of highly increased proinflammatory (IL-1B, IL-6) and suppressed anti-inflammatory (IL-10) cytokines normalised in the SFPG group by days 14 (plasma) and 45 (GCF), but never in the control group. Although not acting directly as antibiotic, SFPG acted in synergy with human granulocytes blocking adaptive catalase induction inS. aureusin response to granulocyte-derived oxidative stress, thus enhancing intracellular bacterial killing.

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