Total Flavones Isolated from Lycium Barbarum L. Inhibit the Proliferation, Migration and Invasion of GlioblastomaU-87MG Cells by Decreasing Formyl Peptide Receptor 1 Expression
Abstract Background: To study the role and mechanisms of total flavones from Lycium barbarum L. (TFL) and FPR1 in the growth of glioblastoma U-87MG cells.Main Methods: CCK-8, wound-healing and Transwell were used for investigating proliferation, motility and invasion of U87 cells after treating with total flavones. RT-qPCR and Western blot were used to study the effect of total flavones on proliferating cell nuclear antigen (PCNA), matrix metalloproteinase2 (MMP2) and FPR1. The short hairpin RNA and FPR agonist fMLP were used to delineate the role of FPR1.Results: TFL was successfully isolated, and its concentration was determined to be 6.205 mg/l. TFL inhibited the proliferation, migration and invasion of U-87MG cells in a time and dose-dependent manner compared to controls. Decreasing FPR1 expression using short hairpin RNA significantly inhibited the migration and invasion of U-87MG cells. Notably, increased expression of FPR1 and treatment with FPR-agonist peptides such as N-formylmethionyl-leucyl-phenylalanine induced the migration and invasion of U-87MG cells, which was significantly decreased when the cells were treated with TFL. Conclusion: TFL inhibits the proliferation, migration and invasion of human glioblastoma U-87MG cells through decreasing the expression of FPR1. These findings provide valuable evidence for the development of antitumor drugs.