scholarly journals Elucidation of new xylose metabolizing pathway in Pseudomonas gessardii VXlt-16 and its correlation with xylitol production from sugarcane bagasse hydrolysate

2021 ◽  
Author(s):  
Vishal Ahuja ◽  
Sanjeev Mehta ◽  
Ranju Kumari Rathour ◽  
Vaishali Sharma ◽  
Nidhi Rana ◽  
...  
Keyword(s):  
Large Scale ◽  
Xylose Reductase ◽  
Xylose Isomerase ◽  
Operating Conditions ◽  
Raw Material ◽  
Xylitol Production ◽  
Sugarcane Bagasse Hydrolysate ◽  
Rdna Sequencing ◽  
High Production ◽  
High Production Cost

Abstract Scientific interventions have identified lignocellulosic biomass as potential raw material for various industrial processes. However toxic byproducts released during the process result in deterioration of environment to a greater extent. Microbes can utilize these wastes for production of products of commercial value like bio-fuels, protein, organic acids and xylitol. However, high production cost and astringent operating conditions have been the major bottlenecks for its commercial production. In microbes, xylose is metabolized by xylose isomerase (XI) and xylose reductase-xylitol dehydrogenase (XR-XDH) pathways, with later having ability to transform pure xylose as well as xylose rich lignocelluloses. Efforts to find hyper producer isolates for xylitol production resulted in identification of one such isolate Pseudomonas gessardii VXlt-16 (MG770460) by 16s rDNA sequencing. Statistical optimization resulted in 7.28 folds’ increase in xylitol yield with 64.76% xylose bioconversion. Conversion of xylose to xylitol even at large scale suggests the possible application of bacterial isolate for the production of this useful product at industrial scale.

scholarly journals Elucidation of New Xylose Metabolizing Pathway in Pseudomonas Gessardii VXlt-16 and its Correlation with Xylitol Production from Sugarcane Bagasse Hydrolysate

2021 ◽  
Author(s):  
Vishal Ahuja ◽  
Sanjeev Mehta ◽  
Ranju Kumari Rathour ◽  
Vaishali Sharma ◽  
Nidhi Rana ◽  
...  
Keyword(s):  
Large Scale ◽  
Xylose Reductase ◽  
Xylose Isomerase ◽  
Operating Conditions ◽  
Raw Material ◽  
Xylitol Production ◽  
Sugarcane Bagasse Hydrolysate ◽  
Rdna Sequencing ◽  
High Production ◽  
High Production Cost

Abstract Scientific interventions have identified lignocellulosic biomass as potential raw material for various industrial processes. However toxic byproducts released during the process result in deterioration of environment to a greater extent. Microbes can utilize these wastes for production of products of commercial value like bio-fuels, protein, organic acids and xylitol. However, high production cost and astringent operating conditions have been the major bottlenecks for its commercial production. In microbes, xylose is metabolized by xylose isomerase (XI) and xylose reductase-xylitol dehydrogenase (XR-XDH) pathways, with later having ability to transform pure xylose as well as xylose rich lignocelluloses. Efforts to find hyper producer isolates for xylitol production resulted in identification of one such isolate Pseudomonas gessardii VXlt-16 (MG770460) by 16s rDNA sequencing. Statistical optimization resulted in 7.28 folds’ increase in xylitol yield with 64.76% xylose bioconversion. Conversion of xylose to xylitol even at large scale suggests the possible application of bacterial isolate for the production of this useful product at industrial scale.

scholarly journals In defense of the old generation: the strategic options to sustain a vintage urea plant production

2018 ◽  
Vol 74 ◽  
pp. 05004
Author(s):  
Diana Mutia Pratiwi ◽  
Sudarna
Keyword(s):  
Agricultural Sector ◽  
Descriptive Analysis ◽  
Process Condition ◽  
High Energy ◽  
Raw Material ◽  
Plant Production ◽  
Feed Ratio ◽  
Urea Fertilizer ◽  
High Production ◽  
High Production Cost

The Need for Urea Fertilizer supply is one of the essential factors for the development of the agricultural sector. However, due to the high energy usage and the increasing price of natural gas as the raw material, some of the old urea fertilizer industry was forced to shut down due to the inability to cover the high production cost. The purpose of this study is to analyze the alternative strategy for the urea industry to be more efficient and also produce good quality urea to remain sustainable. This paper will use descriptive analysis based on the Case Study compared to the literature and related references to find the appropriate and well-implemented alternative. Based on the study, several options can be purposed such as retrofitting in ammonia and urea plant mainly in syn-loop and high-pressure section, and by optimizing the feed ratio and process condition.

scholarly journals Comparative study of sucrose plasticised bioplastic derivatives with selected petro-plastics

2020 ◽  
Vol 12 (1) ◽  
pp. 386-394
Author(s):  
E.C. Agwamba
Keyword(s):  
Mechanical Properties ◽  
High Density Polyethylene ◽  
Production Cost ◽  
Young Modulus ◽  
Raw Material ◽  
Significant Difference ◽  
High Production ◽  
Bulk Matrix ◽  
High Production Cost ◽  
Hcl Concentration

The major setback with most bioplastics is their inherent inability to compete with Petro-plastics in terms of high production cost, and there poor mechanical properties like low tensile strength and percentage extension. This study explore the availability and affordability of mango starch as raw material for bioplastic production and compared some of its mechanical properties with High density Polyethylene (HDPE), Low density polyethylene (LDPE), polyvinyl chloride (PVC), and Polyurethane (PU). Mango starch was used to synthesize bioplastic derivatives, with variable levels of sucrose as plasticiser, aqueous HCl concentration and Carboxymethyl cellulose (CMC) as additive and the mechanical properties of the derived biofilms was measured and compared with the selected Petro-plastics films. It was observed that B1 thermoplastic derivatives have the higher young modulus of 5.658 GPa than that obtained for PVC (4.682 GPa), and PU (3.771 GPa) but show no significant difference and significantly higher than that of HDPE (0.049 GPa), and LDPE (0.063 GPa) (p < 0.05). B2 and B3 indicated a young modulus that is significantly lower than PU and PVC, but showed a young modulus that is higher than LDPE and HDPE with no significant difference (p < 0.05). The FTIR spectra indicate that hydrogen bond was formed in the bulk matrix of the bioplastic derivatives at a band region of 3600 -600 cm-1 wavenumber with broad discrete peaks. Keywords: Petro-plastics; Bioplastics; Mechanical Properties; Plasticization; Mango Starch

A Short Review on Recent Development of Laccase Immobilization on Different Support Materials

2021 ◽  
Vol 02 (01) ◽  
Author(s):  
Norsyafiqah Amalina Ahmad Jafri ◽  
◽  
Roshanida A. Rahman ◽  
Noorhalieza Ali ◽  
◽  
...  
Keyword(s):  
Large Scale ◽  
Cost Effective ◽  
Short Review ◽  
Industrial Applications ◽  
Innovative Strategies ◽  
Support Materials ◽  
High Production ◽  
Efficient Recovery ◽  
High Production Cost ◽  
Laccase Immobilization

Laccase is a bio catalytic agent and multi-copper enzyme containing oxidases that are potentially great for oxidizing large number of phenolic and non-phenolic compounds. However, drawbacks do arise when laccase use in large scale; low in stability, high production cost, non-reusability, sensitive towards denaturing and poor storage ability of free enzymes. These problems lead to the progress in laccase immobilization in order to facilitate the efficient recovery and re-use of the enzyme, thus enabling cost-effective in continuous processes. Apart from discussing on different methods in laccase immobilization such as entrapment, encapsulation and cross-linking in general, we have reviewed a recent development in laccase immobilization on different supports or carriers binding (natural and synthetic). Future works are recommended to focus on innovative strategies on the modified supports to improve the enzyme immobilization as well as sensible entrapment techniques for industrial applications.

Profitability of Sugar Beet Crop in 2018/2019 Campaign on the Example of Lubelskie Province

2020 ◽  
Vol 24 (3) ◽  
pp. 39-49
Author(s):  
Zbigniew Krzysiak
Keyword(s):  
Sugar Beet ◽  
Production Cost ◽  
Complex Analysis ◽  
Indirect Costs ◽  
Raw Material ◽  
Total Production ◽  
Previous Season ◽  
High Production ◽  
High Production Cost ◽  
Sugar Beet Crop

AbstractThe work presents a complex analysis and cost accounting of beet sugar cultivation in 2018/2019 campaign for individual farms of Lublin region. The economic results obtained by producers are mainly affected by indirect costs accounting for 60.11% of the revenue from the total production. Within this group of costs, the major components are sowing service, harvest and soil liming operations reaching 39.38%. Sugar beet production in the analyzed campaign was profitable, with the profitability index of 1.24 and unit production cost 14.33 PLN∙dt−1 is considered one of the profit-making activities in agricultural production, yet it is characterized by a high production cost that gobbled up to 80.39% of the total revenue in the analyzed 2018/19 campaign. The main factor influencing the income from sugar beet cultivation was the price for the raw material, which in the considered business year in relation to the previous season increased by only 0.77 PLN∙t−1.

Optimization of Xylose Reductase production from Citrobacter sp.

2021 ◽  
Vol 16 (8) ◽  
pp. 90-97
Author(s):  
Rashmi Niranjan ◽  
Vishal Ahuja ◽  
Arvind Kumar Bhatt
Keyword(s):  
Large Scale ◽  
Bacterial Isolate ◽  
Healthy Lifestyle ◽  
Xylose Reductase ◽  
Value Added ◽  
Cost Effective ◽  
Xylitol Production ◽  
Straight Chain ◽  
Bone Demineralization ◽  
Value Added Products

Xylitol is a poly-hydroxy straight-chain five-carbon alcohol that can replace sugar in daily uses without any side effects. Lowered risk of dental carries and bone demineralization further support its involvement in a healthy lifestyle. In addition, its role in the synthesis of various commercial products like glycol, ethanol, and resins etc. increases its market value and makes it one of the most valuable bio-products. Microbial fermentation is a cost-effective and eco-friendly method for xylitol production from agricultural residues as available xylose is reduced to xylitol by Xylose reductase (XR) using an equivalent amount of NADPH as a mediator for electron transfer. Previous literature emphasized the use of fungi and yeast for xylitol production rather than bacteria. In contrast to available reports, the potential of the bacterial isolate was evaluated for xylitol production. The effect of process parameters was observed on xylitol yield in terms of XR activity. Out of sixty-eight bacterial isolates obtained, XYLBV-05 was selected for XR production after screening and was identified as Citrobacter sp. based on morphological, microscopic, and biochemical characteristics. Parametric analysis increased the XR production by 4.12 folds (36.61 U/ml). Preliminary results also proved its efficiency in conversion of biomass hydrolysate to xylitol at lab scale but further efforts are needed for xylitol production using agro-industrial lignocellulosic biomass at a large scale which will not only aid in the generation of revenue as a result of value-added products but will also help in environment conservation.

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