scholarly journals Clinical Factors Associated With the Culture of Primary Human Peritoneal Mesothelial Cell From Peritoneal Dialysate Effluent

2021 ◽  
Author(s):  
Ping Shi ◽  
Peiran Yin ◽  
Lingling Liu ◽  
Haixia Zhang ◽  
Ye Zhu ◽  
...  
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Success Rate ◽  
Mesothelial Cell ◽  
Clinical Factors ◽  
Peritoneal Dialysate ◽  
Factors Associated ◽  
Peritoneal Mesothelial Cell ◽  
Human Peritoneal Mesothelial Cell

Abstract Background: The culture of primary human peritoneal mesothelial cell (HPMC) provides us an in vitro tool to investigate peritoneal fibrosis and ultrafiltration failure. The aim of the present study was to establish the method of culturing HPMC and to explore clinical factors associated with the success rate of culture.Methods: HPMCs were aseptically harvested by centrifuge from peritoneal dialysate effluent (PDE) of patients with end-stage renal disease (ESRD) who underwent peritoneal dialysis (PD) catheterization for less than 2 weeks and were cultured in vitro. Cells were identified by simple morphological observation and immunofluorescent staining. Clinical data of PD patients was collected. Comparison between groups and binary logistic regression analysis were employed to explore the clinical factors associated with the success rate of culture.Results: The study included 36 patients (26 male (72.2%); mean age 53.9±15.6 years). HPMC from PDE successfully grew and survived in 22 patients. A typical cobblestone-like appearance was observed by inverted phase contrast microscope. Immunofluorescence staining showed positive expression of cytokeratin-18 (CK-18) and Vimentin. Comparison between groups demonstrated significant differences in diabetes (P=0.041), days from catheterization (P=0.002) and the use of erythrocyte lysate (P=0.019) between the two groups. Multivariate logistic regression analysis revealed that the success rate was correlated with days from catheterization (OR=0.318, 95%CI=0.107-0.946, P=0.039) and the level of C-reactive protein (CRP, OR=0.893, 95%CI=0.805-0.991, P=0.032). Conclusions: The method of culturing primary HPMC from PDE has been successfully established. The success rate of culture is correlated with CRP level and days from catheterization.

scholarly journals Conventional Trabeculotomy versus Gonioscopy-Assisted Transluminal Trabeculotomy: A Retrospective Cohort Study

2021 ◽  
Vol 11 (1) ◽  
pp. 46
Author(s):  
Masashi Takata ◽  
Hiroto Ishikawa ◽  
Tomohiro Ikeda ◽  
Fumi Gomi
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Success Rate ◽  
Logistic Regression Analysis ◽  
Multivariate Logistic Regression Analysis ◽  
Glaucoma Surgery ◽  
Multivariate Logistic Regression ◽  
Surgical Success ◽  
Factors Associated ◽  
Lowering Effect

Background: Conventional trabeculotomy (CT) is performed in an ab-externo manner with at most 120 degrees of incision area of Schlemm’s canal (SC). Recently, gonioscopy-assisted transluminal trabeculotomy (GATT), which makes possible a 360-degree incision area of SC in an ab-interno manner, is introduced. The purpose of this study was to compare surgical outcomes for CT and GATT with and without simultaneous phacoemulsification over 24 months and to identify factors associated with surgical success. Results: Patients’ baseline characteristics were not significantly different between two groups. The surgical success rate in CT and GATT with phacoemulsification groups were 40.4% and 96.6% and were significantly higher in the GATT group than in the CT group (p < 0.001). However, the surgical success rate in CT and GATT without phacoemulsification groups were 40.8% and 54.2%, and there were no significant differences between two groups without phacoemulsification (p = 0.55). Similarly, the postoperative IOP was significantly lower in the GATT group than in the CT group only in eyes with simultaneous phacoemulsification. There were no significant differences in the numbers of glaucoma medications between the two groups. Additional glaucoma surgery was needed in 13.2% and 25.9% of patients in the GATT and CT groups, respectively (p = 0.157). The multivariate logistic regression analysis revealed that the surgical success of trabeculotomy was significantly associated with combined phacoemulsification and the type of glaucoma surgery (GATT). Conclusion: Although both groups without phacoemulsification had a similar surgical success and IOP-lowering effect, GATT combined with phacoemulsification had a higher surgical success rate and a greater IOP-lowering effect compared with combined CT and phacoemulsification. Multivariate logistic regression analysis showed that the factors associated with higher surgical success at one year and two years postoperatively were the combined phacoemulsification procedure and the GATT.

scholarly journals Factors Associated with the Success of Orthodontic Miniscrews

2018 ◽  
Vol 7 (2) ◽  
pp. 55-60 ◽  
Author(s):  
Amir Mohammadi ◽  
Sareh Keshavarz Meshkinfam ◽  
Saeid Foroughi Moghaddam
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Success Rate ◽  
Logistic Regression Analysis ◽  
Age Groups ◽  
Insertion Torque ◽  
Success Rates ◽  
Factors Associated ◽  
Osseointegrated Implants ◽  
Effect Of Age

Background and aims. Nowadays miniscrews are widely used as skeletal anchorage in orthodontics. However, the success rate of miniscrews is less than that of osseointegrated implants. The aim of this retrospective study was to evaluate factors influencing the success rate of orthodontic miniscrews. Materials and methods. Data of 244 miniscrews in 122 patients (99 females and 23 males, with a mean age of 19 years and 6 months) were collected. Logistic regression analysis was used to evaluate the effect of age, gender, placement side and insertion torque on the success rates of miniscrews. Results. The overall success rate of miniscrews was 90.6% in the present study (221/244). Logistic regression analysis showed that the success rate of miniscrews was not under the influence of variables such as gender, placement side and miniscrew brand. However, age groups and insertion torques over 10 Ncm decreased miniscrew success rates. In this context, the success rates of miniscrews in patients under 16 years of age was less than those in patients over 16 years of age (P<0.001) and the success rates of miniscrews with insertion torques ≤10 Ncm were higher than those with insertion torques over 10 Ncm (P=0.019). Conclusion. We concluded that patients under 16 years of age and insertion torques over 10 were increased the failure of orthodontic miniscrews.

scholarly journals Analysis and Factors Associated with Measles in Larut, Matang and Selama Districts, Perak, Malaysia

2020 ◽  
Vol 27 (5) ◽  
pp. 130-140
Author(s):  
Asraf Ahmad Qamruddin ◽  
Reza Qamruddin ◽  
Ayu Malik
Keyword(s):  
Logistic Regression ◽  
Regression Analysis ◽  
Incidence Rate ◽  
Logistic Regression Analysis ◽  
Protective Factor ◽  
Multiple Logistic Regression Analysis ◽  
Prior History ◽  
Multiple Logistic Regression ◽  
Case Definitions ◽  
Factors Associated

Objectives: To determine the incidence rate of measles and the factors associated with confirmed measles cases in Larut, Matang and Selama districts. Methods: Cross-sectional analysis was carried out looking at all suspected and laboratoryconfirmed measles cases in Larut, Matang and Selama districts between 2015 and 2019. Multiple logistic regression analysis was used to determine the associated factors for laboratory-confirmed measles cases. Results: The incidence rate for suspected measles showed an increasing trend from 2015–2019. For laboratory-confirmed measles cases, the incidence rate showed more variation with an increase to 36.11 per million population in 2017 from 5.67 per million population in 2015. The incidence rate later decreased to 10.99 per million population in 2018 and increased again to 24.47 per million population in 2019. From multiple logistic regression analysis, cases that fulfilled the case definition of measles were more likely to be laboratory-confirmed measles. On the other hand, a prior history of measles immunisation was a protective factor. Conclusion: Measles incidence is increasing in trend. Any suspected measles cases that fulfilled the clinical case definitions need to be further investigated. Immunisation should be promoted as they are effective in preventing and eliminating measles.

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