Identification, Functional Analysis and Preliminary Validation of Differentially Expressed Genes in Hyperacute Cerebral Infarction Patients
Abstract Background/AimsAt present, most studies on ischemic stroke and micro RNA are focused on 24h after stroke. Therefore, our experiment intends to analyze the differentially expressed genes in hyperacute cerebral infarction patients, and to analyze the relationship between the most significantly differentially expressed miRNA and clinical characteristics. MethodsDownload miRNA expression microarray and gene expression microarray from GEO database, screen differentially expressed genes and miRNA by bioinformatics method, and analyze their biological functions. The peripheral plasma of patients with hyperacute stroke was collected, the related miRNAs were extracted, and the relationship between them and clinical characteristics was analyzed. ResultsThe microarray included 39 hyperacute cerebral infarction patients patients and 33 healthy volunteers and 5 differentially expressed miRNAs and 1107 differentially expressed genes were obtained by analysis. MiR-3156-5p was selected to further analyze and verify the correlation with the clinical characteristics of patients. The results showed that the expression level of miR-3156-5p was significantly positively correlated with triglyceride level, and had a significant negative impact on stroke. The low level of miR-3156-5p in hyperacute cerebral infarction patients may be one of the initiating factors of neuroinflammation induced by ischemic stroke, and functional analysis and some existing experimental results also support this view. ConclusionsIn our study, a series of differentially expressed genes and differentially expressed miRNAs were screened by analyzing GEO data sets. In addition, the low expression of miR-3156-5p in hyperacute cerebral infarction patients may be one of the initiating factors of neuroinflammation induced by ischemic stroke.