scholarly journals Unexpected Detection of a Sub-millimeter Early Hepatocellular Carcinoma Focus by Intraoperative Near-infrared Fluorescence Imaging

2020 ◽  
Author(s):  
Bo Dai ◽  
Ziyang Wang ◽  
Qian Lu ◽  
Hucheng Ma ◽  
Nida El Islem Guissi ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Real Time ◽  
Tumor Cells ◽  
Near Infrared ◽  
Imaging System ◽  
Residual Tumor ◽  
Cancer Surgery ◽  
Early Hepatocellular Carcinoma ◽  
Nirf Imaging

Abstract Background: Intraoperative near-infrared fluorescence (NIRF) imaging became a great assistance to surgeons for precision cancer surgeries. Here, we demonstrate that NIRF was capable of detecting ultra-small hepatocellular carcinoma focus of about 430µm during real time monitored liver cancer surgery. Prognosis of hepatocellular carcinoma is closely related to residual tumor cells and tissues after tumor resection. Thus, close monitoring to ensure a complete removal of residual tumor is primordial. However, up to now, the identification of tiny lesions has not been reported. Herein, we report our findings about a case where tiny lesions were successfully identified by real-time ICG-NIRF imaging. Case presentation: A 55-year-old man, with chronic hepatitis B infection, was preoperatively diagnosed with liver space-occupying lesion. A fluorescence signal was detected on the surface of the liver through the NIRF imaging system. We then tested the residual liver surface and observed a high signal point, less than 1 mm in the right anterior lobe of the liver. Histopathological examination revealed that the tiny fluorescent spot belongs to an early hepatocellular carcinoma focus. Conclusion: Results strongly suggest that ICG-NIRF imaging system should be used as a routine intraoperative detection method for liver cancer surgery, in order to remove any residual tumor cells and tissue, hence minimizing further risk of remnant tumor regrowth.

scholarly journals NIRF Optical/PET Dual-Modal Imaging of Hepatocellular Carcinoma Using Heptamethine Carbocyanine Dye

2018 ◽  
Vol 2018 ◽  
pp. 1-12 ◽  
Author(s):  
Caiqin Zhang ◽  
Yong Zhao ◽  
Ningning Zhao ◽  
Dengxu Tan ◽  
He Zhang ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Liver Cancer ◽  
Near Infrared ◽  
Imaging Techniques ◽  
Standardized Uptake Value ◽  
Dual Modality ◽  
Imaging Probe ◽  
Orthotopic Xenograft ◽  
Nirf Imaging ◽  
Carbocyanine Dye

Combining near-infrared fluorescence (NIRF) and nuclear imaging techniques provides a novel approach for hepatocellular carcinoma (HCC) diagnosis. Here, we report the synthesis and characteristics of a dual-modality NIRF optical/positron emission tomography (PET) imaging probe using heptamethine carbocyanine dye and verify its feasibility in both nude mice and rabbits with orthotopic xenograft liver cancer. This dye, MHI-148, is an effective cancer-specific NIRF imaging agent and shows preferential uptake and retention in liver cancer. The corresponding NIRF imaging intensity reaches 109/cm2tumor area at 24 h after injection in mice with HCC subcutaneous tumors. The dye can be further conjugated with radionuclide68Ga (68Ga-MHI-148) for PET tracing. We applied the dual-modality methodology toward the detection of HCC in both patient-derived orthotopic xenograft (PDX) models and rabbit orthotopic transplantation models. NIRF/PET images showed clear tumor delineation after probe injection (MHI-148 and68Ga-MHI-148). The tumor-to-muscle (T/M) standardized uptake value (SUV) ratios were obtained from PET at 1 h after injection of68Ga-MHI-148, which was helpful for effectively capturing small tumors in mice (0.5 cm × 0.3 cm) and rabbits (1.2 cm × 1.8 cm). This cancer-targeting NIRF/PET dual-modality imaging probe provides a proof of principle for noninvasive detection of deep-tissue tumors in mouse and rabbit and is a promising technique for more accurate and early detection of HCC.

The near infrared imaging system for the real-time protection of the JET ITER-like wall

2017 ◽  
Vol T170 ◽  
pp. 014027 ◽  
Author(s):  
A Huber ◽  
D Kinna ◽  
V Huber ◽  
G Arnoux ◽  
I Balboa ◽  
...  
Keyword(s):  
Real Time ◽  
Near Infrared ◽  
Infrared Imaging ◽  
Imaging System ◽  
Near Infrared Imaging ◽  
The Real ◽  
Infrared Imaging System

scholarly journals Targeting Contrast Agents With Peak Near-Infrared-II (NIR-II) Fluorescence Emission for Non-invasive Real-Time Direct Visualization of Thrombosis

2021 ◽  
Vol 8 ◽  
Author(s):  
Kenneth S. Hettie
Keyword(s):  
Contrast Agents ◽  
Real Time ◽  
Near Infrared ◽  
Fluorescence Emission ◽  
Therapeutic Window ◽  
Direct Visualization ◽  
Spatiotemporal Resolution ◽  
Accurate Identification ◽  
Non Invasive ◽  
Nirf Imaging

Thrombosis within the vasculature arises when pathological factors compromise normal hemostasis. On doing so, arterial thrombosis (AT) and venous thrombosis (VT) can lead to life-threatening cardio-cerebrovascular complications. Unfortunately, the therapeutic window following the onset of AT and VT is insufficient for effective treatment. As such, acute AT is the leading cause of heart attacks and constitutes ∼80% of stroke incidences, while acute VT can lead to fatal therapy complications. Early lesion detection, their accurate identification, and the subsequent appropriate treatment of thrombi can reduce the risk of thrombosis as well as its sequelae. As the success rate of therapy of fresh thrombi is higher than that of old thrombi, detection of the former and accurate identification of lesions as thrombi are of paramount importance. Magnetic resonance imaging, x-ray computed tomography (CT), and ultrasound (US) are the conventional non-invasive imaging modalities used for the detection and identification of AT and VT, but these modalities have the drawback of providing only image-delayed indirect visualization of only late stages of thrombi development. To overcome such limitations, near-infrared (NIR, ca. 700–1,700 nm) fluorescence (NIRF) imaging has been implemented due to its capability of providing non-invasive real-time direct visualization of biological structures and processes. Contrast agents designed for providing real-time direct or indirect visualization of thrombi using NIRF imaging primarily provide peak NIR-I fluorescence emission (ca. 700–1,000 nm), which affords limited tissue penetration depth and suboptimal spatiotemporal resolution. To facilitate the enhancement of the visualization of thrombosis via providing detection of smaller, fresh, and/or deep-seated thrombi in real time, the development of contrast agents with peak NIR-II fluorescence emission (ca. 1000–1,700 nm) has been recently underway. Currently, however, most contrast agents that provide peak NIR-II fluorescence emissions that are purportedly capable of providing direct visualization of thrombi or their resultant occlusions actually afford only the indirect visualization of such because they only provide for the (i) measuring of the surrounding vascular blood flow and/or (ii) simple tracing of the vasculature. These contrast agents do not target thrombi or occlusions. As such, this mini review summarizes the extremely limited number of targeting contrast agents with peak NIR-II fluorescence emission developed for non-invasive real-time direct visualization of thrombosis that have been recently reported.

Image-guided near-infrared fluorescent sentinel lymph node mapping in human breast cancer

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. e11591-e11591
Author(s):  
S. Troyan ◽  
S. Gibbs-Strauss ◽  
S. Gioux ◽  
R. Oketokoun ◽  
F. Azar ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Optical Imaging ◽  
Real Time ◽  
Near Infrared ◽  
Imaging System ◽  
Sulfur Colloid ◽  
Guided Surgery ◽  
Image Guided ◽  
Nir Fluorescence ◽  
Surgical Field

e11591 Background: Breast cancer surgery is presently performed without real-time image-guidance. We have developed a novel optical imaging system for image-guided surgery that uses invisible near-infrared (NIR) fluorescent light to highlight structures on the surgical field with high sensitivity, specificity, and contrast. We have also performed the first human clinical trial of the imaging system in women undergoing SLN mapping for breast cancer. Methods: We used a portable imaging system with an articulating arm that has 6 degrees of freedom, high power LED light source, custom optics, custom software, and sterile drape. The imaging system provided simultaneous and real-time imaging of color video and NIR fluorescence at up to 15 frames per second. N = 6 women with biopsy- confirmed breast cancer undergoing SLN mapping gave informed consent. All subjects received conventional mapping with Tc-99m sulfur colloid using a handheld gamma probe as well as NIR fluorescence-guided SLN mapping using a mixture of indocyanine green (ICG) diluted to a final concentration of 10 μM in human serum albumin (ICG:HSA). Results: The imaging system was easy to position in the operating room, with the articulating arm providing 50” horizontal reach and 70” vertical reach. Working distance to the patient was 18”. NIR fluorescence excitation was 20 mW/cm2 at 760 nm. NIR-depleted white light was 40,000 lux. A total of 1.6 ml of ICG:HSA was injected intra-tumorally and peri-tumorally and the site massaged for 5 min. 8 of 9 SLNs identified by Tc- 99m sulfur colloid were also identified by NIR fluorescence. However, NIR fluorescence identified an SLN, confirmed to have cancer in it, that was not identified by Tc-99m sulfur colloid. These differences were consistent with asynchrony in the injection techniques. Unlike the gamma-ray probe, NIR fluorescence provided high-resolution, large area optical imaging of the surgical field, and helped guide surgical resection. Conclusions: In this 6-patient pilot study, a novel NIR fluorescence optical imaging system was used for the first time, and provided real-time image-guided surgery for SLN mapping of breast cancer. No significant financial relationships to disclose.

A Hyperspectral Imaging System for Identification of Faecal and Ingesta Contamination on Poultry Carcasses

2003 ◽  
Vol 11 (4) ◽  
pp. 269-281 ◽  
Author(s):  
Kurt C. Lawrence ◽  
William R. Windham ◽  
Bosoon Park ◽  
R. Jeff Buhr
Keyword(s):  
Real Time ◽  
Hyperspectral Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Principal Component ◽  
Ccd Camera ◽  
Sample Population ◽  
Multivariate System ◽  
Ratio Image ◽  
On Line

A method and system for detecting faecal and ingesta contaminants on poultry carcasses were demonstrated. A visible/near infrared monochromator, which measured reflectance and principal component analysis were first used to identify key wavelengths from faecal and uncontaminated skin samples. Measurements at 434, 517, 565 and 628 nm were identified and used for evaluation with a hyperspectral imaging system. The hyperspectral imaging system, which was a line-scan (pushbroom) imaging system, consisted of a hyperspectral camera, fibre-optic line lights, a computer and frame grabber. The hyperspectral imaging camera consisted of a high-resolution charge coupled device (CCD) camera, a prism-grating-prism spectrograph, focusing lens, associated optical hardware and a motorised controller. The imaging system operated from about 400 to 900 nm. The hyperspectral imaging system was calibrated for wavelength, distance and percent reflectance and analysis of calibrated images at the key wavelengths indicated that single-wavelength images were inadequate for detecting contaminants. However, a ratio of images at two of the key wavelengths was able to identify faecal and ingesta contaminants. Specifically, the ratio of the 565-nm image divided by the 517-nm image produced good results. The ratio image was then further processed by masking the background and either enhancing the image contrast with a non-linear histogram stretch, or applying a faecal threshold. The results indicated that, for the limited sample population, more than 96% of the contaminants were detected. Thus, the hyperspectral imaging system was able to detect contaminants and showed feasibility, but was too slow for real-time on-line processing. Therefore, a multivariate system operating at 565 and 517 nm, which should be capable of operating at real-time on-line processing speed, should be used. Further research with such a system needs to be conducted.

A NEAR-INFRARED FLUORESCENT DEOXYGLUCOSE DERIVATIVE FOR OPTICAL IMAGING OF EXPERIMENTAL ARTHRITIS

2009 ◽  
Vol 02 (02) ◽  
pp. 179-187
Author(s):  
XIUPING LIU ◽  
ZHENGMING XIONG ◽  
SHEEN-WOO LEE ◽  
JELENA LEVI ◽  
SHAHRIAR YAGHOUBI ◽  
...  
Keyword(s):  
Optical Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Micro Computed Tomography ◽  
Fdg Uptake ◽  
Nirf Imaging ◽  
Positron Emission ◽  
Quantification Analysis ◽  
Optical Imaging System ◽  
Microct Imaging

The purpose of this study is to investigate whether a near-infrared fluorescence (NIRF) probe, Cy5.5-D-glucosamine (Cy5.5-2DG), can image arthritis in collagen-induced arthritic (CIA) mice. The presence of arthritis was verified by both visual examination and micro-computed tomography (MicroCT) imaging. CIA mice were imaged by a micro-positron emission tomography (MicroPET) scanner one hour after intravenous injection of 2-deoxy-2-[18F]fluoro-D-glucose ([18F]FDG). After radioactivity of [18F]FDG decayed away, Cy5.5-2DG was injected into a lateral tail vein of the mice. Arthritic tissue targeting and retention of Cy5.5-2DG in CIA mice were evaluated and quantified by an optical imaging system. Inflammatory tissue in CIA mice was clearly visualized by [18F]FDG-MicroPET scan. NIRF imaging of Cy5.5-2DG in the same mice revealed that the pattern of localization of Cy5.5-2DG in the arthritic tissue was very similar to that of [18F]FDG. Quantification analysis further showed that [18F]FDG uptake in arthritic tissues at one hour post-injection (p.i.) and Cy5.5-2DG uptakes at different time points p.i. were all well correlated (r2over 0.65). In conclusion, Cy5.5-DG can detect arthritic tissues in living mice. The good correlation between the [18F]FDG uptake and Cy5.5-2DG accumulation in the same arthritic tissue warrants further investigation of Cy5.5-2DG as an approach for assessment of anti-inflammatory treatments.

scholarly journals Near Infrared Fluorescence Imaging to Assess Breast Flap Perfusion and Prevention of Ischemia: Case Report

2019 ◽  
Vol 103 (9-10) ◽  
pp. 473-476
Author(s):  
Ju Yong Cheong ◽  
David Goltsman ◽  
Sanjay Warrier
Keyword(s):  
Fluorescence Imaging ◽  
Near Infrared ◽  
Imaging System ◽  
Skin Flap ◽  
Color Temperature ◽  
Near Infrared Fluorescence ◽  
Clinical Method ◽  
Nirf Imaging ◽  
Near Infrared Fluorescence Imaging ◽  
Flap Perfusion

Introduction: Breast skin flap ischemia and necrosis is a serious consequence of breast reconstruction with reported incidence of 10% to 15%. Current clinical method of assessing breast skin flap perfusion includes combination of skin color, temperature, dermal edge bleeding, and capillary refill. Given the high incidence of ischemia and the presence of certain cohort of patient with greater risk, there is a need for an objective method of assessing the skin flap perfusion. Near infrared fluorescence imaging using indocyanine green is a new technique of assessing skin flap perfusion. We present a first reported case where breast skin flap ischemia was objectively identified intraoperatively by NIRF imaging, and this ischemia was reversed through active intra- and postoperative measures, which was objectively again identified by NIRF imaging. Case presentation: A 36-year-old female patient underwent bilateral nipple sparing mastectomies with immediate reconstruction using tissue expanders. Clinical assessment of the breast skin flap was equivocal. NIRF imaging using SPY imaging system (Novadaq Technologies Inc, Toronto, ON, Canada) showed poor perfusion in the periareolar region. As a result, it was decided to completely deflate the expander, actively warm the skin flap and encourage cutaneous perfusion through use of topical glycerin trinitrate patch. A repeat NIRF imaging 48 hours showed 10-fold increase in perfusion in the skin flap. Conclusion: NIRF imaging is a useful tool in assessing breast skin perfusion and to predict potential ischemia, which can aid in surgeon's management of patient.

scholarly journals Hypericin Exerts Detrimental Effect on Huh-7 As a Delegacy of Hepatocellular Carcinoma: A P53 Dependent Pathway

2020 ◽  
Vol 9 ◽  
pp. 1896
Author(s):  
Maedeh Olya ◽  
Hamid Zaferani Arani ◽  
Amirhossein Shekarriz ◽  
Amirhossein Zabolian ◽  
Hadi Zare Marzouni ◽  
...  
Keyword(s):  
Hepatocellular Carcinoma ◽  
Flow Cytometry ◽  
Liver Cancer ◽  
Cell Line ◽  
Real Time ◽  
Real Time Pcr ◽  
Huh7 Cell ◽  
P53 Expression ◽  
Huh7 Cells ◽  
Huh7 Cell Line

Background: Hepatocellular carcinoma is the most common type of liver cancer which arises from the main cells in the liver. We address many studies investigating anti-cancer role of hypericin, however the proposing corresponding molecular pathway seems to be still a debate. Therefore, the present study aimed to evaluate the apoptotic effect of hypericin on the Huh7 as the liver cancer cell line and its relation with the gate keeper gene P53. Materials and Methods: In this study, the Huh7 cell line and fibroblast cells (as control group) were treated with different concentrations of hypericin for 24 and 48 hours. Detection of cell death was performed by MTT assay and flow cytometry. The expression of bax, bcl2 and p53 mRNAs was evaluated by Real-time PCR. Also, Immunocytochemistry (ICC) analysis was used for further evaluation of P53expression. Results: The results showed that hypericin has a dose-dependent cytotoxic effect on the Huh7 cell line, with no or marginal effect on fibroblastic cells. According to flow cytometry results, about 53%cells underwent apoptosis after exposure to LD50 of hypericin for 24 hours. Real-time PCR data demonstrated that the pro-apoptotic genes Bax and P53 expression level increased. Expectedly ICC results confirmed the up-regulation of P53 proteins in treated samples. Conclusion: Our results indicate the cytotoxicity of hypericin on Huh7 cells by affecting the expression of the gate keeper gene P53; furthermore it is suggested that this herb can be utilized simultaneously with modalities targeting P53 up-regulation or related molecular pathways. [GMJ.2020;9:e1896]

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