scholarly journals Growth Regulator and Axillary Bud Position Effects on in Vitro Establishment of Vitis rotundifolia

HortScience ◽  
1991 ◽  
Vol 26 (3) ◽  
pp. 304-307 ◽  
Author(s):  
◽  
Ronald G. Goldy
Keyword(s):  
Shoot Proliferation ◽  
Axillary Buds ◽  
Position Effects ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Murashige And Skoog Medium ◽  
In Vitro Establishment ◽  
Bud Position ◽  
Different Levels

Four muscadine grape (Vitis rotundifolia Michx.) cultivars (Carlos, Noble, Regale, and Tarheel) were evaluated for their ability to be cultured in vitro. Axillary buds were placed on Murashige and Skoog medium as modified by Chee. Different levels of benzylaminopurine [(BA) 0.5 to 10.0 μm], kinetin [(KIN) 0.5 to 5.0 μm], and thidiazuron [(TDZ) 0.5 to 11.3 μm], and different explant positions were evaluated for their effect on in vitro explant establishment and shoot production. Thidiazuron (2.3 to 4.5 μm) alone or in combination with BA (1.0 to 5.0 μm) or KIN (1.0 or 5.0 μm) was effective for establishing axillary buds. Similar levels were also effective for promoting shoot proliferation. Explants originating from the 10 basal nodes of a shoot with at least 25 nodes gave better shoot proliferation than explants originating from the 10 distal nodes. Chemical names used: 6-benzylaminopurine, 6-furfurylaminopu. rine (kinetin):N -phenyl-N'-l,2,3 -thiadiazol-5-y lurea (thidiazuron).

scholarly journals INCREASED SHOOT PROLIFERATION OF APPLES CO-CULTIVATED WITH AGROBACTERIUM TUMEFACIENS

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 661a-661
Author(s):  
F.A. Hammerschlag ◽  
R.H. Zimmerman ◽  
A.C. Smigocki
Keyword(s):  
Shoot Proliferation ◽  
Shoot Formation ◽  
Pcr Analysis ◽  
Axillary Shoot ◽  
Putative Transformants ◽  
Proliferation Medium ◽  
Shoot Production ◽  
Dna Fragment ◽  
Different Levels

`McIntosh' apple shoots were inoculated in vitro with Agrobacterium tumefaciens strain tms328::Tn5 (tms) carrying a functional cytokinin gene. Callus tissue, removed from the infected stems, produced shoots on shoot proliferation medium. After three subcultures, axillary shoot production from a tms-infected putative transformant was eight times that of controls. Subsequent shoot production on three different levels of BA (3, 6 and 10 uM) was significantly greater than from controls on all levels of BA. PCR analysis of putative transformants revealed an expected 503 bp DNA fragment corresponding to the amplified portion of the cytokinin gene. After 6 months of in vitro propagation, proliferation rates of shoots obtained from the original transformants were similar to the controls and the expected PCR fragment of 503 bp could only be detected by Southern analysis. Even though the T-DNA appears to be lost from the apple genome, the data suggest that the tms strain may be useful in co-infection experiments to induce shoot formation, thus avoiding difficult regeneration procedures.

scholarly journals In Vitro Propagation of Muscadine Grape by Axillary Shoot Proliferation

1990 ◽  
Vol 115 (2) ◽  
pp. 324-329 ◽  
Author(s):  
Ni Lee ◽  
Hazel Y. Wetzstein
Keyword(s):  
Basal Medium ◽  
Shoot Proliferation ◽  
Nodal Segments ◽  
Axillary Shoot ◽  
Axillary Shoot Proliferation ◽  
Vitis Rotundifolia ◽  
Shoot Production ◽  
Muscadine Grape ◽  
Better Than

Plantlets were recovered from axillary bud cultures of muscadine grape (Vitis rotundifolia, `Summit'). Nodal segments 0.5 to 1.0 cm long were cultured in Murashige and Skoog (MS) basal medium supplemented with 5, 10, 20, or 40 μm BA. Best total shoot production was obtained with 10 μm BA; with higher BA levels, shoots were unexpanded and exhibited high mortalities. MS medium supplemented with IBA enhanced rooting by increasing rooting percentage and number per plantlet. Shoots previously proliferated on medium with 5 μm BA rooted significantly better than those multiplied on 10 μM BA. Shoot vigor during rooting was greater in shoots proliferated on 5 vs. 10 μm BA. Root development was not significantly affected by liquid vs. agar-solidifted medium or shoot length. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA), 1H-indole-3-butyric acid (IBA).

scholarly journals Effect of different levels of NAA on in vitro growth and development of shoots of Dendrobium orchid

1970 ◽  
Vol 34 (3) ◽  
pp. 411-416 ◽  
Author(s):  
MS Parvin ◽  
ME Haque ◽  
F Akhter ◽  
M Moniruzzaman ◽  
ABM Khaldun
Keyword(s):  
Growth And Development ◽  
Shoot Proliferation ◽  
Shoot Length ◽  
In Vitro Growth ◽  
Shoot Number ◽  
Number Of Leaves ◽  
Shoot Weight ◽  
Different Levels ◽  
In Vitro Shoot Proliferation

The present study was conducted to investigate the effect of growth regulator NAA on in vitro shoot proliferation, rooting, and plantlet establishment. Among the different concentrations of NAA, the best increase in shoot weight (0.25 g) and shoot number (8.83) were observed from 0.1 mg/I NAA. The highest shoot length (2.60 cm), number of leaves (4.83), number of roots (5.15), and root length (2.67 cm) were obtained with 0.2 mg/I NAA at 60 DAT. Key Words: Dendrobium orchid, NAA, MS media. DOI: 10.3329/bjar.v34i3.3966 Bangladesh J. Agril. Res. 34(3) : 411-416, September 2009

scholarly journals Axillary Shoot Proliferation from in Vitro Culture of Pulmonaria L. Shoots

HortScience ◽  
1996 ◽  
Vol 31 (4) ◽  
pp. 630d-630
Author(s):  
Dennis P. Stimart ◽  
John C. Mather
Keyword(s):  
In Vitro Culture ◽  
Shoot Proliferation ◽  
Axillary Shoot ◽  
Optimum Level ◽  
Axillary Shoot Proliferation ◽  
Skoog Medium ◽  
Murashige And Skoog Medium ◽  
Rooting Response

Actively growing shoots from Pulmonaria L. `Roy Davidson' were cultured in vitro on Murashige and Skoog medium containing benzyladenine (BA) to establish proliferating cultures. BA at 0, 0.4, 0.8, 4.4, 8.8, and 44.4 μm was compared for shoot proliferation and rooting response. Shoot count was highest on 8.8 μm BA with root count highest on 0 or 0.4 μm BA. Subculture 4 weeks later of shoots to the same treatments resulted in highest shoot counts on 44.4 μm BA. Optimum level for micropropagation was 8.8 or 44.4 μm BA. Greatest rooting was at 0 or 0.4 μm BA.

scholarly journals Multiple Shoot Production In Vitro of the Tropical Timber Tree, Sentang (Azadirachta excelsa Linn.)

HortScience ◽  
1998 ◽  
Vol 33 (6) ◽  
pp. 1073-1075 ◽  
Author(s):  
T.K. Liew ◽  
C.K.H. Teo
Keyword(s):  
Sodium Hypochlorite ◽  
Multiple Shoot ◽  
Axillary Buds ◽  
Naphthaleneacetic Acid ◽  
Ms Medium ◽  
Tropical Timber ◽  
Shoot Production ◽  
Timber Tree

Axillary buds from 5-month-old seedlings of Azadirachta excelsa Linn. were surface-sterilized twice with 1.35% (m/v) and 1.05% (m/v) of sodium hypochlorite for 25 and 15 minutes, respectively, before culturing on Murashige and Skoog (MS) medium containing combinations of BA and NAA. A combination of 4.4 μM BA + 0.5 μM NAA induced the most axillary buds to grow (eight per explant). Subsequent proliferation of the micropropagated shoots on this medium yielded abnormal shoots. The best medium for maximum proliferation of these micropropagated shoots contained 3.3 μM BA and 0.27 μM NAA. On this medium about four normal shoots were produced per explant. These findings indicate that two different media are needed for successful micropropagation of sentang. Chemical names used: N6-benzylaminopurine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals Some preliminary results of experiments with in-vitro culture of dipterocarps.

1983 ◽  
Vol 31 (3) ◽  
pp. 233-238
Author(s):  
W.T.M. Smits ◽  
B. Struycken
Keyword(s):  
In Vitro Culture ◽  
Growth Regulators ◽  
Leaf Explants ◽  
Axillary Buds ◽  
Parent Plant ◽  
Preliminary Results ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

A study was made of the influence of light, temp., growth regulators, and sugar and macrosalt concn. on the growth and morphogenesis of leaf and axis explants of young Shorea curtisii, S. obtusa and Dipterocarpus grandiflorus. Terminal and axillary buds grew best on half strength Murashige and Skoog medium. Leaf explants formed more callus on full strength medium, when containing part of the midrib and when taken from the lower half of the leaf. More than 95% of D. grandiflorus explants were infected by a fungus apparently present in the parent plant. (Abstract retrieved from CAB Abstracts by CABI’s permission)

scholarly journals MICROPROPAGATION OF ARONIA ARBUTIFOLIA AND A. MELANOCARPA

HortScience ◽  
1990 ◽  
Vol 25 (9) ◽  
pp. 1096a-1096
Author(s):  
Mark H. Brand ◽  
William G. Cullina
Keyword(s):  
Rapid Growth ◽  
Woody Plant ◽  
Shoot Proliferation ◽  
Breeding Programs ◽  
Murashige And Skoog Medium ◽  
Woody Plant Medium ◽  
Culture Morphology ◽  
Selection Of ◽  
Growing Shoot

Increasing interest in landscape use of Aronia arbutifolia and A. melanocarpa has led to the establishment of breeding programs and selection of improved phenotypes within the genus. A rnicropropagation system was developed to facilitate rapid and easy multiplication of improved forms of Aronia. Actively growing shoot tips of A. arbutifolia `Brilliantissima' and A. melanocarpa were used to initiate shoot proliferation from axillary buds. Optimum proliferation of shoots useful for micropropagation occurred on media supplemented with 0.5 to 1.0 mg 1-1 benzyladenine. Both Murashige and Skoog medium and Woody Plant medium supported vigorous shoot proliferation, but differences in culture morphology were evident. In vitro rooting and non-sterile rooting methods both resulted in high rooting percentages, the formation of numerous roots and subsequent rapid growth of plantlets.

scholarly journals Micropropagation of the Aquatic Plant Cryptocoryne lucens

HortScience ◽  
1990 ◽  
Vol 25 (6) ◽  
pp. 687-689 ◽  
Author(s):  
Michael E. Kane ◽  
Edward F. Gilman ◽  
Matthew A. Jenks ◽  
Thomas J. Sheehan
Keyword(s):  
Polyurethane Foam ◽  
Aquatic Plant ◽  
Shoot Proliferation ◽  
Shoot Multiplication ◽  
Naphthaleneacetic Acid ◽  
Shoot Cultures ◽  
Ex Vitro ◽  
Single Node ◽  
In Vitro Establishment

Procedures for in vitro establishment, rapid shoot proliferation, and ex vitro plantlet acclimatization of Cryptocoryne lucens de Witt were determined. Shoot cultures were established from surface-sterilized shoot tips cultured on Linsmaier and Skoog salts and vitamins medium (LS) solidified with 0.8% (w/v) agar and supplemented with 2.0 μm BA and 0.5 μm NAA. The effect of BA (0 to 20 μm) and 0.5 μm NAA on shoot multiplication from single-node and clustered triple-node shoot explants was determined after 35 days. The most efficient shoot proliferation (7.7 shoots/explant) occurred from single-node shoot explants cultured on LS + 20 μm BA and 0.5 μm NAA. Maximum plantlet establishment was achieved by direct sticking of triple-node (cluster) microcuttings in either soilless planting medium or polyurethane foam cubes. Production of highly branched salable plants from microcuttings was possible within 18 weeks. Chemical names used: N-(phenylmethyl) -1H-purin-6-amine (BA); 1-naphthaleneacetic acid (NAA).

scholarly journals In Vitro Propagation of Apios americana

HortScience ◽  
1990 ◽  
Vol 25 (11) ◽  
pp. 1439-1440 ◽  
Author(s):  
E.R.M. Wickremesinhe ◽  
W.J. Blackmon ◽  
B.D. Reynolds
Keyword(s):  
Gibberellic Acid ◽  
In Vitro Propagation ◽  
Basal Medium ◽  
Shoot Proliferation ◽  
Axillary Buds ◽  
Ms Medium ◽  
Butanoic Acid ◽  
Apios Americana

Shoot proliferation from axillary buds of Apios americana Medikus (apios, groundnut) was obtained on a modified Murashige and Skoog (MS) medium supplemented with 2.22 μm BAP, 0.5 μm IBA, and 3.0 μm GA3. Existed shoots rooted on MS basal medium. About 60% of the rooted plants were successfully established in soil. Chemical names used: 1 H-indole-3-butanoic acid (IBA). gibberellic acid (GA3), N6-benzylaminopurine (BAP).

scholarly journals In Vitro Micropropagation of 54 Species from 15 Genera of Bamboo

HortScience ◽  
1992 ◽  
Vol 27 (5) ◽  
pp. 453-454 ◽  
Author(s):  
P. Prutpongse ◽  
P. Gavinlertvatana
Keyword(s):  
Room Temperature ◽  
Multiple Shoots ◽  
Axillary Buds ◽  
Skoog Medium ◽  
Stem Node ◽  
In Vitro Micropropagation ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Strength Medium

Fifty-four out of 67 species of bamboo tested were successfully propagated in vitro. For nearly every species, multiple shoots were produced from axillary buds on stem node segments cultured on Murashige and Skoog medium containing BA. In a very few species plants could be regenerated adventitiously from callus. This method of propagation was not very efficient or reliable. Rooting occurred in media containing NAA at 2.7 to 5.4 μM. Several species could be stored in vitro on half-strength medium at room temperature > 15 months without transfer. Chemical names used: N6-benzylamino purine (BA); napthyleneacetic acid (NAA).

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