scholarly journals Characterization of sweet cassava accessions based on molecular, quantitative and qualitative data

2011 ◽  
Vol 11 (3) ◽  
pp. 232-240 ◽  
Author(s):  
Eduardo Alano Vieira ◽  
Josefino de Freitas Fialho ◽  
Fábio Gelape Faleiro ◽  
Graciele Bellon ◽  
Kenia Graciele da Fonseca ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Quantitative Traits ◽  
Rapd Markers ◽  
Qualitative Data ◽  
Germplasm Bank ◽  
Qualitative And Quantitative ◽  
Qualitative Characters ◽  
Genetic Dissimilarity ◽  
Sweet Cassava

The purpose of this study was to estimate the genetic divergence in sweet cassava accessions by molecular markers and quantitative and qualitative characters, as well as determine the correlation between these estimates. Sixteen sweet cassava accessions of the Regional Cassava Germplasm Bank of the Cerrado were evaluated under field conditions, for 13 quantitative and 33 qualitative characters. In the laboratory, the accessions were evaluated with RAPD markers. Subsequently, matrixes of genetic dissimilarity/distance among the accessions were estimated based on molecular markers and quantitative and qualitative characters. Besides, the significance of the correlation between the matrixes was estimated. The RAPD, qualitative and quantitative data indicated the existence of high divergence among the accessions. The divergences estimated by molecular markers and by quantitative traits were weakly associated with each other and moderately with the divergence estimated by qualitative characters.

scholarly journals Examination of Apple (Malus × domestica) Fruit Quality and Tree and Fruit Morphology using Molecular Markers

HortScience ◽  
1995 ◽  
Vol 30 (4) ◽  
pp. 832C-832
Author(s):  
Patrick J. Conner ◽  
Susan K. Brown ◽  
Norman F. Weeden
Keyword(s):  
Molecular Markers ◽  
Fruit Quality ◽  
Quantitative Traits ◽  
Rapd Markers ◽  
Fruit Size ◽  
Stem Length ◽  
Fruit Morphology ◽  
Genetic Traits ◽  
Qualitative And Quantitative ◽  
Plant Form

Two half-sib populations (cross 1 = `Wijcik McIntosh' (WM) × NY 75441-67, and cross 2 = WM × NY 75441-58) were used to create maps for the parents and to find RAPD or isozyme markers for qualitative and quantitative traits. WM is a sport of `McIntosh' and is heterozygous for the dominant columnar (Co) gene for reduced branching. WM is of great interest in breeding because of the tremendous effect of the Co gene on many aspects of plant form. NY 75441-67 and NY 75441-58 are advanced selections with commercial fruit quality and resistance to scab (Vf resistance from M. floribunda). Traits examined included both tree (plant height, stem diameter, suckering, branching habit, leaf break, burr knot production) and fruit (size, shape, color, stem length, seed number) characters and fruit quality traits (pH, acid content, Brix). The conservation of RAPD markers in these closely related crosses will be examined and the usefulness of molecular markers to preselect for components of plant form and fruit quality will be discussed. Molecular markers will increase the efficiency of the apple breeding program by aiding the understanding and manipulation of complex genetic traits.

scholarly journals Morphologic and molecular characterization of Myrciaria spp species

2005 ◽  
Vol 27 (3) ◽  
pp. 507-510 ◽  
Author(s):  
Márcio Pereira ◽  
Antonio Luis de Oliveira ◽  
Regina Eli de Almeida Pereira ◽  
Janete Apparecida Desidério Sena ◽  
Juliana Regina Vieira da Costa ◽  
...  
Keyword(s):  
Molecular Markers ◽  
Rapd Markers ◽  
Fruit Trees ◽  
Sao Paulo ◽  
São Paulo ◽  
Paulo State ◽  
Morphologic Characteristics ◽  
Botanical Classification ◽  
Made In

The jabuticaba tree is considered one of the most typical Brazilian fruit trees. However, few studies of this plant are found in the literature and even its botanical classification is very controversial. The present research reports some comparisons between jabuticaba species, using morphologic (organography) and molecular markers techniques. The morphologic characteristics of the plant used as markers were compared with specimens of the herbaria from São Paulo and Minas Gerais States and with the descriptions reported in the literature. Molecular differences between the species were identified by the use of RAPD markers. The experiment was made in Piracicaba, Jaboticabal and Ituverava municipal districts in São Paulo State, Brazil. Morphologic and molecular differences between the studied plants were identified and four groups of species were defined: Myrciaria cauliflora (Mart.) O. Berg, M. coronata Mattos, M. jaboticaba (Vell.) O. Berg. and Myrciaria phytrantha (Kiaersk.) Mattos. Both molecular and morphologic markers techniques showed to be important tools for the identification of jabuticaba tree species.

scholarly journals Genetic evaluation and characterization of jute (Corchorus spp. L) genotypes using DUS

2014 ◽  
Vol 10 (2) ◽  
pp. 147-153 ◽  
Author(s):  
A Das ◽  
D Kumar
Keyword(s):  
Plant Height ◽  
Seed Weight ◽  
Quantitative Traits ◽  
Genetic Advance ◽  
High Heritability ◽  
Qualitative Characters ◽  
Fibre Fineness ◽  
Almost All ◽  
Varietal Characterization

In this study 32 jute (Corchorus spp.) varieties comprised of 18 olitorius and 14 capsularis were evaluated and characterized for five quantitative and 12 qualitative characters as per revised official DUS Test Guidelines of Jute. The experimental materials were grown in a RBD with three replications at Baarrackpore, India during 2009-10. Significant differences among genotypes noted almost for almost all the quantitative traits. PCV and GCV were highest for 1000 seed weight (33.78 and 33.29%) followed by fibre fineness (27.11 and 26.70%). Low variability was recorded in case of plant height. All the five characters exhibited more or less high heritability coupled with high genetic advance as per cent over mean. Out of 12 qualitative characters studied, in C. capsularis 8 traits were monomorphic, 4 traits were dimorphic. However, in C. olitorius no trait was monomorphic, 6 traits were dimorphic and 6 traits were polymorphic among varieties indicating their potential for varietal characterization. DOI: http://dx.doi.org/10.3329/sja.v10i2.18335 SAARC J. Agri., 10(2): 147-153 (2012)

scholarly journals MOLECULAR MARKERS FOR CHARACTERIZATION OF GRAPE CULTIVARS II

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 660f-660
Author(s):  
Y. Gogorcena ◽  
S. Arulsekar ◽  
D.E. Parfitt
Keyword(s):  
Molecular Markers ◽  
Extraction Method ◽  
Rapd Markers ◽  
Pinot Noir ◽  
Specific Reference ◽  
Series Of Experiments ◽  
Rapd Makers ◽  
Grape Cultivars

The work reported here is an extension of studies reported in 1990. The general objective was to develop molecular markers for genotype `fingerprinting', with specific reference to possible clonal differences among `Pinot noir' clones. Leaf DNA from 8 cultivars and 9 `Pinot noir' clones were isolated. RFLP and RAPD markers were identified and used to characterize the genotypes. 65 32-P labelled cloned probes were constructed with the pUC18 plasmid and Hind-III digested `Pinot noir' DNA. The probes were tested for their ability to discriminate among the 8 cultivars. 3 probes pGAD10, pGAD15, and pGAD44 showed polymorphisms among the cultivars. pGAD15 was most useful, with 5 polymorphisms for the 8 cultivars. RAPD makers were also tested for `fingerprinting'. Several primers were tested and polymorphisms were identified among cultivars. However, significant problems with repeatability for some bands were observed. Therefore, a series of experiments were conducted to test the effect of season and extraction method. These factors did not account for the inconsistancy which seemed to be more a function of the primer used. None of these studies showed clear evidence that the `Pinot noir' clones tested were geetically different.

scholarly journals COMPARATIVE ANALYSIS RESULT OF LOCAL PADDY KINSHIP BASED ON QUANTITATIVE AND QUALITATIVE CHARACTERS

Agric ◽  
2017 ◽  
Vol 29 (2) ◽  
pp. 89-102
Author(s):  
Wage Ratna Rohaeni ◽  
Nani Yunani
Keyword(s):  
Genetic Distance ◽  
Quantitative Character ◽  
Phenotypic Characterization ◽  
Component Level ◽  
Kinship Analysis ◽  
Qualitative And Quantitative ◽  
Genotype Information ◽  
Qualitative Characters ◽  
Using Data

Morpho-agronomic characters consist of two types of qualitative and quantitative characters. Qualitative characters were discontinuous and score data form, while the quantitative are continuous and measurable. Kinship analysis is important for breeders to know the genetic distance between genotypes. Allegedly there are differences in the genetic kinship analysis between based on data from qualitative to quantitative character. The aim of this study was to compare the results of the analysis of kinship based on quantitative and qualitative data on phenotypic characterization of local rice. A total of 43 local rice from West Java was used on phenotypic characterization activities. The research was done at Sukamandi experiment station of ICRR (Indonesian Rice Research Institute) on 2014. Qualitative characters were observed as many as 24 characters, and quantitative as many as 23 characters. Analysis of data was by using Minitab version 14. The results indicate that kinship analysis results using data from qualitative, quantitative or a combination of both was not significantly different at the component level analysis of the number of clusters and similarity but there were real differences in the level of genetic distance. Dendogram based on qualitative characters was different than based on quantitative character. Analysis of kinship between genotype recommended using a data combination of qualitative and quantitative character that the genetic distance between genotype information more accurately

Genetic Diversity of Sesame Based on Qualitative and Quantitative Traits and Molecular Markers

2015 ◽  
Vol 19 (2) ◽  
pp. 273-296
Author(s):  
R. M. Fahmy ◽  
Abeer A. H. Elhalwagi ◽  
Sahar F. M. El-Hefnawy
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Quantitative Traits ◽  
Qualitative And Quantitative

scholarly journals Identification and characterization of morphological diversity of Lemba (Curculigo latifolia) in East Kalimantan, Indonesia

2017 ◽  
Vol 18 (4) ◽  
pp. 1367-1376
Author(s):  
INCE RADEN ◽  
CANDRA CATUR NUGROHO ◽  
SYAHRANI SYAHRANI
Keyword(s):  
Morphological Traits ◽  
Quantitative Traits ◽  
Phenotypic Variability ◽  
Morphological Diversity ◽  
Qualitative Traits ◽  
Qualitative And Quantitative ◽  
East Kalimantan ◽  
Quantitative And Qualitative Traits ◽  
Identification And Characterization

Raden I, Nugroho CC. Syahrani. 2017. Identification and characterization of morphological diversity of Lemba (Curculigo latifolia) in East Kalimantan, Indonesia. Biodiversitas 18: 1367-1376. Lemba (Curculigo latifolia) is Kalimantan’s land prospective for commercial cultivation because its leaf fiber has been traditionally utilized as fiber source for ulap doyo woven cloth; its fruit as sweetener; and its root as medicinal herb. To this days, only a few of research has been conducted on lemba in East Kalimantan or even in Indonesia, including studies on lemba plant phenotype suitable for weaving material. Thus, a research about identification and morphological characterization of lemba cultivars is necessary to identify and characterize lemba plant in East Kalimantan to reveal phenotypic variability in both quantitative and qualitative traits that can be utilized as the source of germplasm for the plant breeding program. This study was carried out in three Sub-districts, i.e.: Tenggarong Sub-district (Kutai Kartanegara District), Kota Bangun Subdistrict (Kutai Kartanegara District), and Jempang Sub-district (West Kutai District). Data collection was done by observation, and measurement of quantitative and qualitative traits of lemba plant. Descriptive analysis was applied on the morphological traits data, while correlation analysis was performed to reveal relationship between the qualitative and quantitative morphological traits by using software Minitab v.14.12. Phenotypic variability was determined based on the standard deviation. Principal component analysis and cluster analysis of the data were performed by using software SPSS v.11.6. The variance value of the 33 observed lemba plant accessions from the three Sub-districts indicated that the phenotypic variability of the qualitative traits was narrow while that of the quantitative traits was broad. There are three principal components that account for the observed variability with a cumulative variance of 96% which is obtained from 19 observed traits. Cluster analysis at similarity 95% indicates that there are five lemba accessions groups based on only qualitative, only quantitative, or based on combined qualitative and quantitative traits.

scholarly journals GENETIC CHARACTERIZATION OF GALOBA DURIAN (AMONUM SPP.) IN AMBON ISLAND BASED ON RANDOM AMPLIFIED POLYMORPHIC DNA (RAPD)

2018 ◽  
Vol 3 (1) ◽  
pp. 27-33
Author(s):  
Fuadiska Salamena ◽  
Cecilia Anna Seumahu
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Genetic Characterization ◽  
Kidney Diseases ◽  
Random Amplified Polymorphic Dna ◽  
Quantitative Result ◽  
Dna Amplification ◽  
Polymorphic Band ◽  
Qualitative And Quantitative

Galoba durian is one of the endemic floras from Maluku. Galoba durian is a species belonging to the Amomum genera Zingiberaceae family. It is also used as a medicinal plant for waist and kidney diseases. Based on the color of the fruit, galoba durian is divided into two nmely red galoba durian and green galoba durian. Distribution of this plant in Ambon can found in a few places such as highland and coastal area. Different locations influence phenotypic of plants, but may not show different genetic characteristic. Genetic diversity can detected by molecular markers. Genetic characterization from galoba durian using RAPD markers has not been done before. This study aimed to analyze genetic diversity from galoba durian using molecular markers RAPD. Samples of plants are used red galoba durian from Hatu and green galoba durian from Hatalae. The result of the first study, characterization of the morphology of the galoba durian, showed that both galoba have almost similar characteristics. Further DNA was tested by qualitative and quantitative. Result shows good qualitative and quantitative of DNA genomic. The second study was amplification by PCR-RAPD. DNA amplifications were performed using 3 primers out of 9 screened random primers. The primers selection was based on hight polymorphism. DNA amplification has 36 bands which were 100% polymorphic. The size of each bands from visualization of agarose was determined by linear regression. Number of band amplified was range from 120 to 1612 bp. Polymorphic band of RAPD showed the highest  genetic diversity. It can be concluded that the two plants of galoba durian are different species

scholarly journals Phenotypic characterization of Native Chicken reared under intensive management system

1970 ◽  
Vol 8 (1) ◽  
pp. 79-82 ◽  
Author(s):  
S Faruque ◽  
NU Siddiquee ◽  
MA Afroz ◽  
MS Islam
Keyword(s):  
Management System ◽  
Quantitative Traits ◽  
Reproductive Traits ◽  
Phenotypic Characterization ◽  
Intensive Management ◽  
Phenotypic Characteristics ◽  
Qualitative And Quantitative ◽  
Native Chicken ◽  
Native Chickens

The Phenotypic characteristics of three genotypes of native chicken comprising of Non-descript Desi (ND), Hilly (H) and Naked Neck (NN) were studied. A total of 180 individual chickens (Male: ND= 20; H= 20 and NN=20; Female: ND= 40, H=40 and NN = 40) were characterized under intensive management system for qualitative and quantitative traits. The results indicated that the predominant plumage color of three types of native chickens was black brownish (33.33%) followed by white with black tips (28.33 %) and red brownish (18.33%). Values indicate that 35% of native chickens had whitish shank color followed by yellowish 31.68%; black 11.66 % and others 21.67 %. All hens of ND, H and NN laid light brown (62.42%) to cream or off white (30.28%) colored eggs. Variations were also found on quantitative traits such as shank length, body weight and reproductive traits on intensive management system. number of eggs/ hen from starting to ten months of laying were 108, 104 and 112, respectively in ND, H and NN genotypes. Three native chicken genotypes showed distinct physical variations for both qualitative and quantitative traits under intensive management system. Keywords: Native chicken; Quantitative traits; Qualitative traitsDOI: 10.3329/jbau.v8i1.6402J. Bangladesh Agril. Univ. 8(1): 79-82, 2010

scholarly journals Characterization of potato genotypes using molecular markers

2004 ◽  
Vol 39 (9) ◽  
pp. 871-878 ◽  
Author(s):  
Eliana Antonia Silveira Collares ◽  
Eva Choer ◽  
Arione da Silva Pereira
Keyword(s):  
Molecular Markers ◽  
Gel Electrophoresis ◽  
Rapd Markers ◽  
Polyacrylamide Gel Electrophoresis ◽  
Group Method ◽  
Jaccard Coefficient ◽  
Leaf Extracts ◽  
Arithmetic Average ◽  
Pair Group

The objective of this work was to characterize 27 potato genotypes, using molecular markers. Polyacrylamide gel electrophoresis, RAPD techniques and isozymes of esterase, phosphoglucomutase and soluble proteins were analyzed in tubers, and isocitrate dehydrogenase, aspartate transaminase, phosphoglucomutase and peroxidase, in leaves. Eighteen primers were tested and four were chosen, kits OPX (01, 04 and 09) and OPY (07), to analyze RAPD markers in leaf extracts. Similarity and cluster analysis were conducted using Jaccard coefficient and the unweighted pair-group method using arithmetic average. Despite the differences detected in the analysis of proteins and isozymes in the tubers, as well as of isozymes in the leaves, the characterization of all genotypes through gel electrophoresis was not possible, while RAPD markers were efficient to characterize all the 27 genotypes.

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