scholarly journals Somatic embryogenesis of hypocotyl derived calli from an eggplant cultivar

2020 ◽  
Vol 115 (1) ◽  
pp. 151
Author(s):  
Hajar SABET ◽  
Mahmood MALEKI ◽  
Maryam ABDOLI NASAB ◽  
Saeid MIRZAEI
Keyword(s):  
Plant Regeneration ◽  
Culture Medium ◽  
Shoot Elongation ◽  
Gene Transformation ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Hypocotyl Explants ◽  
Somaclonal Variations ◽  
Micro Propagation ◽  
Embryogenic Callus Induction

<p>Optimization of tissue culture and regeneration conditions of eggplant is necessary for achieving different goals such as gene transformation and the development of somaclonal variations. In this study, hypocotyl explants ware used to produce callus in a medium containing different concentrations of NAA and BAP. Moreover, the concentration of the elements Ca, Mn, Mg, Fe and K were measured and analysed between embryogenic and non-embryogenic calli. For shoot elongation, embryogenic calli were transferred to a new culture medium containing 3.5, 4 and 4.5 mg l<sup>-1</sup> BAP plus 2 mg l<sup>-1</sup> GA3. Finally, produced shoots were rooted in a culture medium containing 1, 1.5 and 2 mg l<sup>-1</sup> NAA. Results showed that the best treatment for the embryogenic callus induction was MS medium containing 0.5 mg l<sup>-1</sup> BAP plus 0.25 mg l<sup>-1</sup> NAA. Two elements, Fe and K, had the highest amount in non-embryogenic calli compare to the embryogenic one. For plant regeneration, MS medium containing 4.5 mg l<sup>-1</sup> BAP plus 2 mg l<sup>-1</sup> GA3 and 2 mg l<sup>-1</sup> NAA were the best treatments for shooting and rooting, respectively. In this study, the best treatments for plant regeneration produced 35 shoots from an explant with 92 % shooting. This regeneration protocol could be useful for gene transformation and micro-propagation studies.</p>

scholarly journals DEVELOPMENT OF TRANSFORMATION SYSTEM FOR COFFEA ARABICA USING PARTICLE GUN METHOD

HortScience ◽  
1992 ◽  
Vol 27 (6) ◽  
pp. 661d-661 ◽  
Author(s):  
Chifumi Nagai ◽  
Zhuping Mai ◽  
Jerek Jong
Keyword(s):  
Somatic Embryos ◽  
Coffea Arabica ◽  
Culture Medium ◽  
Gene Transformation ◽  
Ms Medium ◽  
Particle Gun ◽  
Embryogenic Calli ◽  
Blue Mountain ◽  
Secondary Culture ◽  
Leaf Disks

Somatic embryogenesis of coffee has been studied for the purpose of obtaining target tissues for stable genetic transformation through use of a particle gun. Eight cultivars of C. arabica were selected for callus induction from leaves. Primary calli were induced within two weeks in over 98% of the leaf disks explanted on MS medium with 2,4-D and kinetin prior to treatment on a secondary culture medium. Somatic embryos were obtained from `Catuai' and `Blue Mountain' after six months from explanting. Somatic embryos were germinated in MS media and developed into plants. Somatic embryos and embryogenic calli are being used for gene transformation experiments with the helium-driven particle gun.

scholarly journals Peculiarities of induction of callusogenesis and morphogenesis in fennel depending on the age of seedlings

2019 ◽  
pp. 101-108
Author(s):  
A. Sh. Tevfik ◽  
N. A. Yegorova
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Culture Medium ◽  
Hypocotyl Explants ◽  
Breeding Material ◽  
Morphogenetic Potential ◽  
Embryogenic Callus Induction ◽  
Improved Methods

The influence of the age of seedlings (of which hypocotyl explants were isolated) and the composition of the culture medium on the callusogenesis and morphogenesis induction of fennel cultivars ‘Mertsishor’ and ‘Oksamit Kryma’ were studied. It was established that with the use of younger seedlings (7-day of age) callus induction began in a larger number of explants for a week of cultivation earlier compared to 14 and 21-day seedlings. The frequency of somatic embryogenesis in the callus obtained from 7-day seedling hypocotyl was almost twice higher than that of the callus from more mature seedling. It was revealed that the cultivar ‘Mertsishor’ had a higher morphogenetic potential compared to ‘Oksamit Kryma’. Improved methods of embryogenic callus induction and plant regeneration can be used for obtaining the initial breeding material of fennel.

scholarly journals 582 PB 091 PLANT REGENERATION FROM EMBRYOGENIC CALLUS TISSUES OF KENTUCKY BLUEGRASS

HortScience ◽  
1994 ◽  
Vol 29 (5) ◽  
pp. 515c-515
Author(s):  
Shanqiang Ke ◽  
Chiwon W. Lee
Keyword(s):  
Plant Regeneration ◽  
Light Exposure ◽  
Poa Pratensis ◽  
Shoot Proliferation ◽  
Kentucky Bluegrass ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Albino Plants ◽  
Proliferation Medium ◽  
Callus Tissues

Coleoptile tissues from dark-germinated seedlings of Kentucky bluegrass (Poa pratensis L.) cv. Touchdown were excised and cultured on MS medium supplemented with 1.5-2.5 mg/liter picloram plus 0.2 mg/liter benzyladenine (BA) or with 4 mg/liter 2,4-D. Embryogenic calli were formed on media containing 1.5 mg/liter picloram plus 2.5 mg/liter 2,4-D in the dark. When these embryogenic calli were subcultured on MS medium containing either 0.15-0.3 mg/liter picloram or 0.2-0.5 mg/liter 2,4-D in a 16-h day/8-h night photoperiod, 10.5% of the cultures regenerated shoots. Pretreatment of cultures in the dark for 2 weeks prior to light exposure slightly increased the plant regeneration efficiency to 15.5%. Pigmentation of the regenerants varied with a ratio of 8.5 completely green: 2.5 green plus albino: 1 completely albino plants. The shoots were multiplied in the medium containing 0.5 mg/liter BA plus either 0.2 mg/liter picloram or 0.1 mg/liter indoleacetic acid (IAA). Over 90% cultures in the shoot proliferation medium produced roots after 4 weeks.

scholarly journals Bauhinia forficata link shoot regeneration: histological analysis of organogenesis pathway

2000 ◽  
Vol 43 (4) ◽  
pp. 431-431 ◽  
Author(s):  
Marcia O. Mello ◽  
Murilo Melo ◽  
Beatriz Appezzato-da-Glória
Keyword(s):  
Plant Regeneration ◽  
Culture Medium ◽  
De Novo ◽  
Shoot Elongation ◽  
Small Cells ◽  
Indirect Organogenesis ◽  
Shoot Bud ◽  
Half Strength ◽  
Bauhinia Forficata

Plant regeneration was achieved from cells of callus induced from hypocotyl segments of Bauhinia forficata on half strength Murashige and Skoog culture medium supplemented with several concentrations of BAP. Within 40 days of culture shoot buds formation was observed on callus surface. Calli were then transferred to a same composition culture medium without plant growth regulator in order to induce shoot elongation. Histological studies indicated that in vitro plant regeneration in B. forficata occurred through indirect organogenesis. Meristemoids consisting of small cells with dense cytoplasm and prominent nuclei were randomly distributed throughout the callus surface indicating early stages of shoot bud differentiation. Shoots developed de novo from superficial layers of cells and the pattern of shoot origin and development were very similar to those previously described for other leguminous species.

scholarly journals An efficient regeneration system through in vitro somatic embryogenesis of barley (Hordeum vulgare L.)

2019 ◽  
Vol 27 ◽  
pp. 89-99
Author(s):  
M Haque ◽  
SMS Islam
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Casein Hydrolysate ◽  
Ms Medium ◽  
Regeneration System ◽  
Embryogenic Calli ◽  
Hordeum Vulgare L ◽  
The Media ◽  
Barley Genotypes

This study was carried out to improve an efficient protocol for in vitro callus induction and plant regeneration using Bangladeshi barley genotypes collected from BARI, Gazipur, Bangladesh. After sterilization embryos were separated carefully from mature seeds of six barley genotypes (BB-1, BB-2, BB-3, BB-4, BB-5 and BB-6) and cultured them in MS medium supplemented with various concentration and combination of PGRs for callus induction and regeneration. Out of six genotypes BB-6 showed highest (38.17%) callus induction in MS + 4.0 mg/l 2,4-D + 200 mg/l L-proline + 300 mg/l casein hydrolysate; whereas, BB-4 and BB-5 showed no callus induction in the same medium. For plant regeneration from embryogenic calli the same genotype (BB-6) also performed the best results (19.25%) in MS medium supplemented with 1.5 mg/l BAP + 30 g/l sucrose. Analysis of variance (ANOVA) showed highly significant differences among the media and the genotypes. J. bio-sci. 27: 89-99, 2019

scholarly journals Potential Embryogenic Callus Induction Protocol Through Cell Suspension Culture For High Frequency Plant Regeneration Of Maspine Pineapple (Ananas comosus L.)

1970 ◽  
Vol 3 (2) ◽  
pp. 40-45
Author(s):  
M.F. Mohamad Bukhori ◽  
Norzulaani Khalid ◽  
Ch'ng Lou Ven
Keyword(s):  
Plant Regeneration ◽  
Suspension Culture ◽  
Cell Suspension ◽  
Cell Suspension Culture ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Ananas Comosus ◽  
Ms Medium ◽  
Embryogenic Callus Induction

To explore the potential for embryogenic callus induction protocol through cell suspension culture forhigh frequency plant regeneration of Maspine pineapple (Ananas comosus L.), eight different culturemedia formulation were evaluated for their effects on the induction of somatic embryos from suckerexplants. Explants were cultured on MS medium supplemented with various media concentration(NAA, Dicamba and BAP, Picloram, Kinetin and NAA, 2,4-D, TDZ, and TDZ and BAP).Embryogenic callus induction percentage, color and texture of the callus were assessed after fivemonths of culture. The optimum medium for the proliferation of in vitro shoots from sucker explantswas MS medium supplemented with 3 mg/L BAP. Meanwhile, the optimum medium for the inductionof fastest and high percentage of embryogenic callus growth from in vitro leaf-based was MS mediumsupplemented with Picloram. Results of mean comparison showed that 3 mg/L Picloram were moreeffective on explants than 10 mg/L. Results of the double staining method proved that somaticembryogenesis occurred in MS supplemented with 3 mg/L Picloram. Under microscopic observations,the globular-stage of the embryos were revealed in callus cells which is relatively suitable forsuspension cells inoculums, indicating that the tested PGR were significantly effective for somaticembryogenesis formation in this species. Most embryogenic callus from sucker explants wasyellowish-mucilaginous-wet-friable. The developed protocol potentially leads to the production ofembryogenic callus from sucker explants and plant regeneration through somatic embryogenesis.

scholarly journals Plant regeneration from protoplasts of Gentiana straminea Maxim

2016 ◽  
Vol 11 (1) ◽  
pp. 55-60 ◽  
Author(s):  
Guomin Shi ◽  
Lina Yang ◽  
Tao He
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Protoplast Culture ◽  
Dichlorophenoxyacetic Acid ◽  
Ms Medium ◽  
Fresh Weight ◽  
Embryogenic Calli ◽  
2,4 Dichlorophenoxyacetic Acid ◽  
Solid Liquid

AbstractA protocol is described for plant regeneration from protoplasts of Gentiana straminea Maxim. via somatic embryogenesis. Protoplasts were isolated from embryogenic calli in an enzyme solution composed of 2% Cellulase Onozuka R-10, 0.5% Macerozyme R-10, 0.5% Hemicellulase, and 0.5 M sorbitol with a yield of 3.0 × 106 protoplasts per gram of fresh weight. Liquid, solid-liquid double layer (sLD) and agar-pool (aPL) culture systems were used for protoplast culture. The aPL culture was the only method that produced embryogenic, regenerative calli. With aPL culture, the highest frequencies of protoplast cell division and colony formation were 39.6% and 16.9%, respectively, on MS medium supplemented with 2 mg/L 2,4-dichlorophenoxyacetic acid (2,4-D) and 0.5 mg/L N6-benzylaminopurine (BA). Microcalli were transferred to solid MS medium containing a reduced concentration of 2,4-D (0.5 mg/L) to promote the formation of embryogenic calli. Somatic embryos developed into plantlets on MS medium supplemented with 2 mg/L BA at a rate of 43.7%.

scholarly journals Embryogenic Callus Induction and Regeneration of Elite Bangladeshi Indica Rice Cultivars

1970 ◽  
Vol 17 (1) ◽  
pp. 65-70 ◽  
Author(s):  
ME Hoque ◽  
MS Ali ◽  
NH Karim
Keyword(s):  
Plant Regeneration ◽  
Callus Induction ◽  
Embryogenic Callus ◽  
Callus Formation ◽  
Indica Rice ◽  
Rice Cultivars ◽  
Embryogenic Calli ◽  
Rice Callus ◽  
Embryogenic Callus Induction ◽  
Embryogenic Callus Formation

Significant variations were observed among six elite Bangladeshi Indica rice cultivars tested in relation to total callus induction frequency (p = 0.017), embryogenic callus formation frequency (p = 0.001) and subsequent plant regeneration responses (p = 0.005). In all the cases, embryogenic callus formation frequency was much more less than the total callus (embryogenic + non-embryonegic) formation frequency. The embryogenic calli derived from mature seed embryos produced green plants, successfully established in soil and produced fertile seeds.Key words: Indica rice, Callus induction, Plant regeneration, Genotypic variationsDOI = 10.3329/ptcb.v17i1.1122Plant Tissue Cult. & Biotech. 17(1): 65-70, 2007 (June)

scholarly journals Plant regeneration through somatic embryogenesis of yacón [smallanthus sonchifolius (poepp. and endl.) H. Robinson]

2009 ◽  
Vol 52 (3) ◽  
pp. 549-554 ◽  
Author(s):  
Cynthia Manyra Corrêa ◽  
Graciele Nicolodi de Oliveira ◽  
Leandro Vieira Astarita ◽  
Eliane Romanato Santarém
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Ms Medium ◽  
Embryogenic Calli ◽  
Efficient System ◽  
Smallanthus Sonchifolius ◽  
Tuberous Roots ◽  
Medicinal Use ◽  
Half Strength

Smallanthus sonchifolius has tuberous roots containing large amounts of fructo-oligosaccharides and its medicinal use has increased due to the hypoglycemic properties reported for this species. An efficient system for propagation via somatic embryogenesis is reported using petiole segments cultivated on MS medium supplemented with combinations of BA, kinetin and 2,4-D, under light and darkness conditions. Embryogenic callus was formed in most of the treatments; however, somatic embryogenesis was promoted by the presence of light. Clusters of somatic embryos appeared on callus surface after 50 days of culture. The highest number of embryos was produced on 0.45 µM BA and 4.5 µM 2,4-D. Embryogenic calli were maintained on MS medium containing 4.5 µM BA and 0.045 µM 2,4-D. Embryos converted on hormone-free half-strength MS medium with 2 g.L-1 activated charcoal and plantlets were transferred to non-sterile conditions for acclimatization, showing 100% of survival.

scholarly journals Somatic Embryogenesis and Plant Regeneration from Cotyledon and Hypocotyl Explants of Fagopyrum esculentum Moench lpls Mutant

Agronomy ◽  
2019 ◽  
Vol 9 (11) ◽  
pp. 768
Author(s):  
Yue Fei ◽  
Lan-Xiang Wang ◽  
Zheng-Wu Fang ◽  
Zhi-Xiong Liu
Keyword(s):  
Somatic Embryogenesis ◽  
Plant Regeneration ◽  
Somatic Embryos ◽  
Basal Medium ◽  
Fagopyrum Esculentum ◽  
Cereal Crop ◽  
Embryogenic Calli ◽  
Hypocotyl Explants ◽  
Indirect Somatic Embryogenesis ◽  
Fagopyrum Esculentum Moench

Buckwheat (Fagopyrum esculentum, Family Polygonaceae) is an annual pseudo-cereal crop with healing benefits. However, the genetic improvement of common buckwheat has achieved only limited success, mainly due to buckwheat’s dimorphic flowers and heteromorphic self-incompatibility. Here, we develop a useful protocol for indirect somatic embryogenesis and subsequent plant regeneration from hypocotyl explants of F. esculentum. Firstly, the initial calli of hypocotyl explants were induced on Murashige and Skoog (MS) basal medium containing 2.0 mgL−1 2,4-D and 1.5 mgL−1 6-BA for 30 days culture, and then the yellowish white friable embryogenic calli were developed when the initial calli were transferred to fresh MS basal medium supplemented with 1.0 mgL−1 6-BA and 0.5 mgL−1 thidiazuron (TDZ)two to three times subculture at 40–60 days intervals. Subsequently, the somatic embryos were able to germinate from embryogenic callus sub-cultured on MS basal medium containing 1.0 mgL−1 6-BA and 0.5 mgL−1 TDZ with 15% potato puree for 20 days subculture. Finally, maximum mean percentage (75.75%) of somatic embryo-derived plants were obtained when the mature somatic embryos were transferred to MS basal medium without growth regulators for 40 days culture. Our result provides a useful protocol for plant regeneration and SE from hypocotyl explants of F. esculentum.

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