Characterization of Alstroemeria Species using Random Amplified Polymorphic DNA (RAPD) Analysis
Keyword(s):
Rapd Pcr
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In this study, 11 species, hybrids, and color variants were characterized using randomly amplified polymorphic DNA (RAPD) analysis. Total genomic DNA was extracted using a 2% CTAB extraction buffer using fresh or frozen leaf material. The DNA was amplified using standard RAPD-PCR protocols utilizing 10-mer primers. All primers utilized exhibited a high degree of polymorphism in their banding patterns among the species and hybrids studied. The primers used produced ≈40 reproducible bands. It was possible to identify and uniquely distinguish all species and hybrids investigated using these bands.
2009 ◽
Vol 75
(8)
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pp. 2259-2265
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2004 ◽
Vol 50
(1)
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pp. 193-198
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2016 ◽
Vol 97
(6)
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pp. 1307-1315
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2014 ◽
Vol 9
(9)
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pp. 543-555
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