scholarly journals Vegetative Propagation of Mature Eastern and Carolina Hemlocks by Rooted Softwood Cuttings

HortScience ◽  
2005 ◽  
Vol 40 (5) ◽  
pp. 1469-1473 ◽  
Author(s):  
Robert M. Jetton ◽  
John Frampton ◽  
Fred P. Hain
Keyword(s):  
Root Length ◽  
Root Formation ◽  
Tsuga Canadensis ◽  
Adventitious Root Formation ◽  
Eastern Hemlock ◽  
Stem Cuttings ◽  
Total Root Length ◽  
Auxin Concentration ◽  
Cutting Length ◽  
Percent Mortality

This study tested the effects of cutting length and auxin (NAA) concentration on adventitious root formation in softwood stem cuttings from mature eastern hemlock, Tsuga canadensis (L.) Carr., and carolina hemlock, T. caroliniana Engelm. Overall rooting percentage (41%) and percent mortality (22%) were higher for eastern hemlock compared with carolina hemlock (10% rooting and 13% mortality). Rooting percentage of each species responded differently to varying auxin concentrations (0, 1, 2, 4, 8 mm NAA). Maximum rooting (56%) for eastern hemlock occurred at 0 mm NAA; then decreased with increasing auxin concentration. Carolina hemlock rooting percentage increased from the control to a maximum (16%) at 1 mm NAA; then decreased with increasing auxin concentration. For both species, the lowest mortality occurred at the same auxin concentration as maximum rooting. The highest rates of mortality coincided with the same concentrations as the lowest rooting percentages. At all auxin concentrations, eastern hemlock had a higher number of roots and greater total root length relative to carolina hemlock. Mortality among 6-cm stem cuttings was twice that observed for 3-cm cuttings of both species. However, 6-cm cuttings of eastern hemlock that did form adventitious roots had more roots and longer total root length compared with 3-cm cuttings. Chemical name used: 1-naphthalenacetic acid.

scholarly journals Effects of Auxin Concentration and Medium Temperature on Four Woody Plant Taxa Propagated by Stem Cuttings using Recirculating Subirrigation in a Growth Chamber

2003 ◽  
Vol 21 (3) ◽  
pp. 126-130
Author(s):  
James S. Owen ◽  
William A. Johnson ◽  
Brian K. Maynard
Keyword(s):  
Root Formation ◽  
Woody Plant ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Root Number ◽  
Medium Temperature ◽  
Root Numbers ◽  
Auxin Treatment ◽  
Auxin Concentration ◽  
Propagation Medium

Abstract Four woody plant taxa (‘Sparkleberry’ holly, ‘Mariesii’ viburnum, ‘Shasta’ viburnum, and Red Sunset® maple) were propagated from softwood stem cuttings in a recirculating subirrigation propagation system to evaluate the effect on adventitious root formation of three auxin (Dip-n-Grow®) concentrations [0 (water), 20: 1, or 10: 1] and three medium temperatures [20C (68F), 23C (74F), or 26C (79F)]. All taxa showed a positive rooting response to auxin treatment. Compared to cuttings treated with water, root number of all taxa doubled when treated with the highest auxin concentration (10: 1 Dip-n-Grow® dilution). Length of the longest root of rooted cuttings increased with auxin treatment of two taxa. Medium temperature also affected rooting of all taxa. The intermediate temperature evaluated, 23C (74F), was optimal for all rooting parameters on cuttings of ‘Mariesii’ viburnum, and for root length and percentage rooting on cuttings of ‘Shasta’ viburnum and Red Sunset® maple. A propagation medium temperature of 26C (79F) resulted in the greatest root number on cuttings of ‘Shasta’ viburnum, while 20C (68F) was optimal for root number on cuttings of Red Sunset® maple. Root numbers on cuttings of ‘Mariesii’ viburnum were reduced at 20C (68F) and 26C (79F), which appeared to be sub-and supra-optimal temperatures, respectively, for root initiation. All rooting parameters on cuttings of ‘Sparkleberry’ holly increased with propagation medium temperature. Temperature-auxin interactions were apparent for root number and length on cuttings of ‘Mariesii’ and ‘Shasta’ viburnum. The recirculating subirrigation system was useful for evaluating the basal temperature and auxin dose interactions and was effective for rooting softwood stem cuttings without intermittent mist irrigation.

scholarly journals Improving the Rooting Capacity of Stem Cuttings of Virginia Pine by Severe Stumping of Parent Trees

2006 ◽  
Vol 30 (4) ◽  
pp. 172-181 ◽  
Author(s):  
Christopher L. Rosier ◽  
John Frampton ◽  
Barry Goldfarb ◽  
Frank A. Blazich ◽  
Farrell C. Wise
Keyword(s):  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Needle Length ◽  
Root Angle ◽  
Crown Position ◽  
Primary Roots ◽  
Auxin Concentration ◽  
Partial Crown ◽  
Rooting Capacity

Abstract Two experiments were conducted to develop a protocol for rooting stem cuttings from 5-year-old Virginia pine (Pinus virginiana Mill.). The first experiment tested the effects of stumping treatment (none and ¼, ½, and ¾ original height remaining), auxin type[indole-3-butyric acid or 1-napthalene acetic acid (NAA)], and auxin concentration (0, 2, 4, 6, 8, or 12 mM) on adventitious root formation. Stumping refers to the process of cutting back the stem combined with partial crown removal. The second experiment quantified the effects of crown position on the rooting of stem cuttings from stumped and nonstumped trees. Rooting percentage increased and percentage of mortality decreased as the severity of the stumping treatment increased. Auxin concentration significantly affected every rooting trait except root angle. Overall,cuttings from trees stumped to ¼ original height and treated with 4 mM NAA rooted in higher percentages (74%) and produced more primary roots (5.5) with greater total root lengths (601 mm) than the other treatments. The original crown position, particularly height, significantly affected rooting percentage. Primary needle length significantly increased as the severity of the stumping treatment increased and was positively correlated with rooting capacity. South. J. Appl. For. 30(4):182–187.

scholarly journals Growth Stage, Auxin Type, and Concentration Influence Rooting of Stem Cuttings of Fraser Fir

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1397-1402 ◽  
Author(s):  
Christopher L. Rosier ◽  
John Frampton ◽  
Barry Goldfarb ◽  
Frank A. Blazich ◽  
Farrell C. Wise
Keyword(s):  
Root Length ◽  
Growth Stage ◽  
Growth Stages ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Total Root Length ◽  
Fraser Fir ◽  
Root Angle ◽  
Primary Roots ◽  
Percent Mortality

Seven concentrations of IBA and seven concentrations of NAA plus a nonauxin control were tested over three growth stages to determine their effectiveness in promoting adventitious root formation on stem cuttings taken from 3- and 4-year-old stock plants of Fraser fir [Abies fraseri (Pursh) Poir.]. Cuttings were prepared in March (hardwood), June (softwood), or November (semi-hardwood) 2001, treated with auxin concentrations ranging from 0 to 64 mm, and placed under mist. Rooting percentage, percent mortality, number of primary roots, total root length, root system symmetry, and root angle were recorded after 16 weeks. Growth stage and auxin concentration significantly affected every rooting trait except root angle. NAA significantly increased the number of primary roots and total root length. However, auxin type did not significantly affect rooting percentage or percent mortality. The highest rooting percentages (99%) occurred when softwood cuttings were treated with 5 mm auxin, however, semi-hardwood cuttings also rooted at high percentages (90%) and had no mortality when treated with 14 mm auxin. Regardless of auxin type, the number of primary roots and total root length varied in similar patterns across concentration, although, NAA tended to induce a greater response. To root Fraser fir stem cuttings collected from 3- and 4-year-old stock plants, it is recommended that a concentration of 5 mm NAA should be used on softwood cuttings and 14 mm IBA on semi-hardwood cuttings. Chemical names used: indole-3-butyric acid (IBA); 1-naphthaleneacetic acid (NAA).

scholarly journals Growth Stage, Auxin Type, and Concentration Influence Rooting of Virginia Pine Stem Cuttings

HortScience ◽  
2004 ◽  
Vol 39 (6) ◽  
pp. 1392-1396 ◽  
Author(s):  
Christopher L. Rosier ◽  
John Frampton ◽  
Barry Goldfarb ◽  
Farrell C. Wise ◽  
Frank A. Blazich
Keyword(s):  
Root Length ◽  
Growth Stage ◽  
Adventitious Rooting ◽  
Root Diameter ◽  
Growth Stages ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Total Root Length ◽  
Root Angle ◽  
Percent Mortality

Seven concentrations of indole-3-butyric acid (IBA), seven concentrations of 1-naphthaleneacetic acid (NAA), and a nonauxin control were tested over three growth stages to determine their ability to promote adventitious rooting of stem cuttings from 3- and 4-year-old stock plants of virginia pine (Pinus virginiana Mill.). Cuttings were harvested September 2000 (semi-hardwood), February 2001 (hardwood), June 2001 (softwood), and October 2001 (semi-hardwood), treated with auxin concentrations ranging from 0 to 64 mm and placed under intermittent mist in a greenhouse. Rooting percentage, percent mortality, number of primary roots, total root length, root symmetry, root angle, and root diameter were assessed following 16 weeks. Growth stage affected every rooting trait measured except root symmetry and diameter. Auxin type affected total root length and root diameter, while auxin concentration affected every rooting trait except root angle. The highest predicted rooting percentages (46%) occurred when semi-hardwood cuttings were collected in September 2000 and treated with 7 mm auxin. Cuttings collected within the same growing season (2001) exhibited the highest predicted rooting percentage (33%) when softwood cuttings were treated with 6 mm auxin. Semi-hardwood cuttings rooted in 2001 produced the greatest number of roots and root lengths. Root diameter was significantly greater when NAA rather than IBA was applied, especially at higher concentrations.

scholarly journals Short Term Drought Conditioning Influences Adventitious Rooting of Firebush and Fraser's Photinia Stem Cuttings

2004 ◽  
Vol 22 (3) ◽  
pp. 144-148 ◽  
Author(s):  
Lindsey Fox ◽  
Thayne Montague
Keyword(s):  
Plant Growth ◽  
Growth Regulator ◽  
Root Length ◽  
Plant Growth Regulator ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Rooting ◽  
Adventitious Root Formation ◽  
Stem Cuttings ◽  
Short Term

Abstract Experiments investigated the influence of drought conditioning stock plants and an auxin-based plant growth regulator (PGR) on adventitious root formation of terminal stem cuttings taken from containerized firebush and Fraser's photinia stock plants. Drought conditioning (DC) treatments were initiated in a glass greenhouse and included: irrigation every day (no DC), irrigation every other day (medium DC), and irrigation every fourth day (high DC). Plants were subjected to DC for 16 consecutive days. Following DC treatments, all plants were irrigated and left overnight. Terminal stem cuttings were taken the following day and an 0.3% auxin talc formulation was used as the PGR. Plant growth regulator/DC treatments included: no DC ± PGR, medium DC ± PGR, and high DC ± PGR. Cuttings were placed under intermittent mist and heating pads maintained media temperature. After 21 and 78 days cuttings were evaluated for rooting percentage, number of roots, and length of the longest root. For firebush, rooting percentage was near 100% for all treatments. Number of roots and length of longest root for firebush cuttings was greatest for cuttings treated with PGR. For Fraser's photinia cuttings, rooting percentage was highest for cuttings treated with high DC + no PGR treatments. Cuttings treated with medium DC or high DC ± PGR treatment produced the greatest number of roots, while mean root length was longest for cuttings treated with medium DC + PGR and high DC + no PGR.

scholarly journals Gene expression profiling during adventitious root formation in carnation stem cuttings

BMC Genomics ◽  
2015 ◽  
Vol 16 (1) ◽  
Author(s):  
Carlos Villacorta-Martín ◽  
Ana Belén Sánchez-García ◽  
Joan Villanova ◽  
Antonio Cano ◽  
Miranda van de Rhee ◽  
...  
Keyword(s):  
Gene Expression ◽  
Gene Expression Profiling ◽  
Expression Profiling ◽  
Adventitious Root ◽  
Root Formation ◽  
Adventitious Root Formation ◽  
Stem Cuttings

scholarly journals Auxin, Wounding, and Propagation Medium Affect Rooting Response of Stem Cuttings of Arbutus andrachne

HortScience ◽  
2001 ◽  
Vol 36 (5) ◽  
pp. 976-978 ◽  
Author(s):  
Mohammed M. Al-Salem ◽  
Nabila S. Karam
Keyword(s):  
Root Formation ◽  
Adventitious Root Formation ◽  
Chemical Form ◽  
Naphthaleneacetic Acid ◽  
Stem Cuttings ◽  
Acid Form ◽  
Root Number ◽  
Propagation Medium ◽  
Salt Forms ◽  
Rooting Response

Stem cuttings of Arbutus andrachne L. were taken from basal or terminal portions of branches in October and treated with acid or salt forms of IBA or NAA at several concentrations. Also, the effects of wounding and propagation medium were assessed. Auxin was necessary for root formation, IBA acid being most effective. In general, salt forms of IBA and NAA were less effective than acid forms. Concentration of auxin had a significant effect on rooting regardless of auxin type or chemical form. The greatest rooting percentage, root number, length, and fresh and dry weights were exhibited by basal cuttings treated with 24 mm IBA. Position of the cutting on the branch did not affect rooting except when the IBA acid form was used; basal cuttings were then superior to terminal ones. Wounding, by making two opposite longitudinal incisions at the base of the cutting, increased rooting. The propagation medium had a significant effect on adventitious root formation, which was enhanced with increasing perlite percentage in the medium up to 100%. Chemical names used:1H-indole-3-butyric acid (IBA); α-naphthaleneacetic acid (NAA).

scholarly journals Vegetative Propagation of Mountain Fly Honeysuckle (Lonicera villosa) by Overhead Mist and Subirrigation

HortScience ◽  
2019 ◽  
Vol 54 (5) ◽  
pp. 916-919
Author(s):  
Darren J. Hayes ◽  
Bryan J. Peterson
Keyword(s):  
Root Formation ◽  
Dry Weight ◽  
Adventitious Root Formation ◽  
Organic Soils ◽  
Stem Cuttings ◽  
System Quality ◽  
Root Tips ◽  
Root Volume ◽  
Root Dry Weight ◽  
Propagation Medium

We assessed adventitious root formation on stem cuttings of mountain fly honeysuckle [Lonicera villosa (Michx.) Schult.] in separate experiments using overhead mist and subirrigation systems. The concentration of applied potassium salt of indole-3-butyric acid (K-IBA) and the proportions of coarse perlite and milled peatmoss in the propagation medium were varied within both systems. Across treatments, 98% of cuttings in the overhead mist system and 85% of cuttings in the subirrigation system produced roots. In the overhead mist system, root volume, root dry weight, and number of root tips were greatest among cuttings treated with 4000 to 12,000 mg·L−1 K-IBA and stuck into 100% perlite. In the subirrigation system, root dry weight was not significantly affected by K-IBA concentration, but the greatest root volume and number of root tips were produced by cuttings treated with 8000 or 12,000 mg·L−1 K-IBA and stuck into 100% perlite. Despite the natural affinity of mountain fly honeysuckle for moist, organic soils, all of the 18 rooted cuttings we planted in a landscape trial survived and grew appreciably with minimal care over 2 years in a mineral field soil. We conclude that cuttings of mountain fly honeysuckle can be propagated readily by overhead mist or subirrigation, that root system quality is improved substantially by increasing K-IBA concentration and using coarse perlite without peatmoss, and that mountain fly honeysuckle can be grown in typical horticultural landscapes.

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