scholarly journals Influence of Indolebutyric Acid Potassium Salt on Propagation of Semi-hardwood Stem Cuttings of Bougainvillea

HortScience ◽  
2006 ◽  
Vol 41 (4) ◽  
pp. 983D-983
Author(s):  
Christopher B. Cerveny ◽  
James L. Gibson
Keyword(s):  
Potassium Salt ◽  
Lateral Roots ◽  
Tropical Species ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Percent Survival ◽  
Industry Standard ◽  
Primary Roots ◽  
Napthaleneacetic Acid ◽  
Primary And Lateral Roots

Bougainvillea glabra is a tropical species with reportedly difficulty to propagate. Previous research has shown the importance of talc-based rooting hormones when propagating Bougainvillea, yet little has been published on the efficacy of liquid-based formulations. Therefore, our objective was to determine the optimum concentration of indolebutyric acid potassium salt (KIBA) needed to effectively root semi-hardwood stem cuttings of Bougainvillea `California Gold' and `Helen Johnson'. Sub-terminal cuttings measuring 6.5 cm were harvested from stock plants of Bougainvillea on 3-week intervals from 6 June to 8 Aug. and repeated 6 Sept. to 8 Nov. 2005. Cuttings were dipped 0.5 cm in a solution of 0, 1500, 3000, or 6000 mg·L-1 KIBA or in a 1500-mg·L-1 solution containing indolebutyric acid (IBA) 1%: napthaleneacetic acid (NAA) 0.5% and propagated under mist. Cuttings were evaluated for percent survival, rooting quality (1 = poor; 5 = best), and number of primary and lateral roots 5 weeks after planting (WAP). Differences in `California Gold' for percent survival, average rank, and number of roots were determined not significant at P ≤ 0.05. However, application of rooting hormone to `Helen Johnson' increased rooting quality, number of primary roots, and number of lateral roots by up to 24%, 53%, and 50%, respectively. Results indicated rooting performance was generally improved with application of KIBA; therefore, cuttings of Bougainvillea may benefit from a 1500-mg·L-1 solution. KIBA was also found to be as effective as the industry standard liquid formulation. Growers will have to consider the availability and cost of KIBA when propagating Bougainvillea.

scholarly journals INFLUENCE OF STEM CUTTING LOCATION ON PROPAGATION OF MURRAYA PANICULATA AND TECOMA STANS

HortScience ◽  
2006 ◽  
Vol 41 (3) ◽  
pp. 500F-501
Author(s):  
Christopher B. Cerveny ◽  
James L. Gibson ◽  
James E. Barrett
Keyword(s):  
Physiological Age ◽  
Stem Length ◽  
Seasonal Effect ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Percent Survival ◽  
Harvest Dates ◽  
Murraya Paniculata ◽  
Stem Quality ◽  
Napthaleneacetic Acid

Orange Jasmine (Murraya paniculata L. Jack) and Texas Star [Tecoma stans (L.) Juss.] are two tropical ornamentals which have become popular in the specialty floriculture crop market because of their outstanding flower characteristics. Unfortunately they are difficult to root and little has been published on how to propagate them effectively. Therefore, the objective of our experiment was to determine the optimum physiological age of stem tissue necessary to effectively root 2-node stem cuttings. Forty-five cm shoots of Murraya were harvested on 27 June and 7 Sept. 2005, and divided into 2-node stem cuttings representing the top, middle, and bottom sections of the stem (soft-wood, semi-hardwood, and hardwood, respectively). Cuttings were measured for stem length and diameter, dipped in a 1,500 mg·L–1 solution containing indolebutyric acid (IBA) 1%: napthaleneacetic acid (NAA) 0.5%, and propagated under mist for 10 weeks in a 4 perlite: 1 vermiculite substrate (by volume). Tecoma followed a similar regime but were harvested once on 13 Sept. and evaluated 4 weeks after planting. Both species were evaluated for percent survival and rooting quality on a 1 to 5 scale; 1 = poor, 5 = best. Stem quality differences in Tecoma cuttings were shown, but did not influence rooting performance or percent survival. Murrayacuttings indicated a similar trend suggesting that age of tissue is not an important factor when propagating these species. However, when comparing the two harvest dates, data from Murraya cuttings showed an increase in survival from 79% and 95% and an increase in rooting quality from 2.72 to 4.26 when harvested in June compared to Sept., respectively. Cuttings harvested in Sept. were also shown to be 17% shorter with a 126% larger diameter than those harvested in June. These data suggest a trend toward a seasonal effect when harvesting cuttings of Murraya paniculatain Florida. Further studies should be conducted to verify this trend and to identify the ideal season for propagation.

scholarly journals Kosteletzkya virginica Can be Rooted from Leafy or Leafless Stem Cuttings

2004 ◽  
Vol 22 (4) ◽  
pp. 173-175
Author(s):  
Cynthia L. Haynes ◽  
William R. Graves
Keyword(s):  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
Single Leaf ◽  
Primary Roots ◽  
Kosteletzkya Virginica ◽  
Herbaceous Perennial

Abstract Protocols for producing Virginia mallow [Kosteletzkya virginica (L.) K. Presl. ex A. Gray] are needed to allow growers to meet the emerging demand for this herbaceous perennial. Virginia mallow has been propagated from seeds and by division, but the potential for using stem cuttings has not been evaluated. Two experiments were conducted to determine how indolebutyric acid (IBA) treatment affects rooting percentage and the number and length of primary roots on stem cuttings taken from different positions on stock plants. Rooting percentage was similar (mean = 68%) among single-leaf cuttings from nonterminal, distal positions on stock plants and leafless cuttings from basal positions. Averaged over these nonterminal cutting types, IBA treatment more than doubled rooting percentage, root count, and the length of the longest root. Terminal cuttings with leaves rooted more successfully (83%) than subtending cuttings with leaves during a second experiment, and IBA effects were less pronounced than during the first experiment. Results demonstrate that stem cuttings with or without leaves can be used to propagate Virginia mallow efficiently. Application of IBA is not necessary but enhances rooting and appears most beneficial for leafless cuttings.

scholarly journals Impact of soil compaction heterogeneity and moisture on maize (Zea mays L.) root and shoot development

2008 ◽  
Vol 54 (No. 12) ◽  
pp. 509-519 ◽  
Author(s):  
B. Konôpka ◽  
L. Pagès ◽  
C. Doussan
Keyword(s):  
Zea Mays ◽  
Soil Moisture ◽  
Soil Compaction ◽  
Statistical Tests ◽  
Lateral Roots ◽  
Primary Root ◽  
Leaf Biomass ◽  
Fine Soil ◽  
Primary Roots ◽  
Primary And Lateral Roots

Soil compaction heterogeneity and water content are supposed to be decisive factors influencing plant growth. Our experiment focused on simulation of two soil moisture levels (0.16 and 0.19 g/g) plus two levels of clod proportion (30 and 60% volume) and their effects on root and leaf variables of maize (<I>Zea mays</I> L.). We studied number of primary and lateral roots as well as primary root length at the particular soil depths. Statistical tests showed that the decrease rate of the number of roots versus depth was significantly affected by the two studied factors (<I>P</I> < 0.01). Soil moisture and clod occurrence, interactively, affected leaf biomass (<I>P</I> = 0.02). Presence of clods modified root morphological features. Particularly, the diameter of primary roots in the clods was significantly higher than of those grown in fine soil (<I>P</I> < 0.01). For primary roots, which penetrated clods, branching density decreased considerably for the root segments located just after the clods (<I>P</I> = 0.01). Regarding their avoidance to clods and tortuosity, large differences were found between primary roots grown in the contrasting soil environments.

Geoperception in primary and lateral roots of Phaseolus vulgaris (Fabaceae). II. Intracellular distribution of organelles in columella cells

1984 ◽  
Vol 62 (5) ◽  
pp. 1090-1094 ◽  
Author(s):  
J. Steven Ransom ◽  
Randy Moore
Keyword(s):  
Phaseolus Vulgaris ◽  
Lateral Roots ◽  
Relative Volume ◽  
Precise Location ◽  
Primary Roots ◽  
Columella Cells ◽  
Primary And Lateral Roots ◽  
Cellular Components

A morphometric analysis of the ultrastructures of columella cells in primary and lateral roots of Phaseolus vulgaris was performed to determine the precise location of cellular components in these cells. Roots were fixed in situ to preserve the in vivo ultrastructure of the cells. All cellular components in columella cells of both types of roots were distributed asymmetrically. The nucleus and vacuome were located primarily in the middle third of both types of columella cells. Dictyosomes, mitochondria, and amyloplasts were most abundant in the lower third of the columella cells in both types of roots. The distribution of amyloplasts was the most asymmetrical of all cellular components examined, with the lower third of the columella cells containing approximately 90% of the relative volume of amyloplasts in both types of roots. The distribution of cellular components in columella cells of primary roots was not significantly different from that of columella cells of lateral roots. These results indicate that differences in georesponsiveness of primary and lateral roots of P. vulgaris are probably due to factors other than the ultrastructures of their individual columella cells.

scholarly journals Low pH Enhances Rooting of Stem Cuttings of Rhododendron in Subirrigation

1998 ◽  
Vol 16 (1) ◽  
pp. 4-7
Author(s):  
Thomas A. Holt ◽  
Brian K. Maynard ◽  
William A. Johnson
Keyword(s):  
Low Ph ◽  
Stem Cuttings ◽  
Solution Ph ◽  
Indolebutyric Acid ◽  
Sphagnum Peat ◽  
Napthaleneacetic Acid

Abstract Semi-hardwood terminal stem cuttings of Rhododendron L. ‘P.J.M.’, R. ‘Catawbiense Album’ and R. ‘Purple Gem’ were treated with a solution of 0.1% indolebutyric acid and 0.5% napthaleneacetic acid and inserted into perlite for rooting under subirrigation, without overhead mist, at solution pH of 4.5 or 7.5. All cultivars rooted in higher percentages and produced larger root balls at pH 4.5 than at pH 7.5. A second study with softwood stem cuttings of R. ‘P.J.M.’ and both softwood and semi-hardwood stem cuttings of R. ‘Purple Gem’ confirmed these findings and included an additional subirrigation treatment, a sphagnum peat slurry, pH 4.1, which produced nearly identical results to the pH 4.5 treatment.

scholarly journals Vegetative Propagation of Oconee Azalea (Rhododendron flammeum) by Stem Cuttings and Mound Layering

2010 ◽  
Vol 28 (2) ◽  
pp. 69-73
Author(s):  
Jeff R. Jones ◽  
Anthony V. LeBude ◽  
Thomas G. Ranney
Keyword(s):  
Vegetative Propagation ◽  
Potassium Salt ◽  
Root Systems ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
High Quality ◽  
Optimal Method ◽  
Large Root ◽  
Nursery Crops

Abstract Deciduous azaleas (Rhododendron L.) offer a range of desirable ornamental characteristics and can be valuable nursery crops. Availability in the nursery trade, however, can be limited by the lack of effective propagation protocols. Therefore, the objectives of this research were to develop and optimize vegetative propagation protocols for Oconee azalea, Rhododendron flammeum (Michx.) Sarg., utilizing stem cuttings or in-field mound layering. An optimal method for producing rooted stem cuttings with large root systems (&gt; 20 cm2) was to collect softwood stem cuttings from hedged stock plants followed by treatment with 10,000 ppm of the potassium salt (K-salt) of indolebutyric acid (K-IBA). Mound layering was also effective. Fifty percent of the stems on each mound resulted in rooted layers and approximately six layers were produced per mound regardless of mounding season (March or June), with or without wounding, or application of 5000 ppm K-IBA to stems prior to mounding in June. Both softwood cuttings and mound layering can be utilized to produce high quality plants.

scholarly journals Effect of Root-zone Temperature on Survival, Growth, and Root Morphology of Kalmia latifolia and Ilex crenata ‘Compacta’

2007 ◽  
Vol 25 (2) ◽  
pp. 73-77
Author(s):  
Amy N. Wright ◽  
Stuart L. Warren ◽  
Frank A. Blazich
Keyword(s):  
Root Morphology ◽  
Root Zone ◽  
Lateral Roots ◽  
Dry Weight ◽  
Stem Cuttings ◽  
Root Zone Temperature ◽  
Kalmia Latifolia ◽  
Percent Survival ◽  
Mountain Laurel ◽  
Zone Temperature

Abstract Root-zone temperature (RZT) is an important environmental factor affecting growth and performance of woody ornamental plants in the landscape. Research was conducted to compare the effects of RZT on survival, growth, and root morphology of a difficult-to-transplant species, mountain laurel (Kalmia latifolia L.), and an easy to transplant species, Japanese holly (Ilex crenata Thunb.). Seedlings of mountain laurel or micropropagated liners of mountain laurel (Kalmia latifolia L. ‘Sarah’) and rooted stem cuttings of Japanese holly (Ilex crenata Thunb. ‘Compacta’) were grown hydroponically for 12 weeks in controlled environment conditions under long days at 9-hr days/15-hr nights of 26/22C (79/72F) with RZTs of 16, 24, or 32C (61, 75, or 90F). Compared to 16 and 24C (61 and 75F), percent survival of mountain laurel was reduced by a RZT of 32C (90F), whereas percent survival of Compacta holly was unaffected by RZT. Root dry weight of mountain laurel was reduced 72% at 32C (90F) while top dry weight was unaffected by RZT. Top and root dry weights of Compacta holly were unaffected by RZT. Root: top ratio of mountain laurel was reduced by increasing RZT, whereas root: top ratio of Compacta holly was unaffected by RZT. Root area of mountain laurel and Compacta holly were reduced 80 and 64%, respectively, at 32C (90F) compared with 16C (61F). Number of lateral roots in the apical 2 cm (0.8 in) of primary roots of both taxa increased with increasing RZT. Results of this research indicate that reducing RZT in the landscape may increase survival and root growth of transplanted mountain laurel.

Geoperception in primary and lateral roots of Phaseolus vulgaris (Fabaceae). III. A model to explain the differential georesponsiveness of primary and lateral roots

1985 ◽  
Vol 63 (1) ◽  
pp. 21-24 ◽  
Author(s):  
J. Steven Ransom ◽  
Randy Moore
Keyword(s):  
Phaseolus Vulgaris ◽  
Concentration Gradient ◽  
Lateral Root ◽  
Lateral Roots ◽  
Primary Root ◽  
Growth Inhibitors ◽  
Gravitropic Response ◽  
Primary Roots ◽  
Primary And Lateral Roots

Half-tipped primary and lateral roots of Phaseolus vulgaris bend toward the side of the root on which the intact half tip remains. Therefore, tips of lateral and primary roots produce growth effectors capable of inducing gravicurvature. The asymmetrical placement of a tip of a lateral root onto a detipped primary root results in the root bending toward the side of the root onto which the tip was placed. That is, the lesser graviresponsiveness of lateral roots as compared with primary roots is not due to the inability of their caps to produce growth inhibitors. The more pronounced graviresponsiveness of primary roots is positively correlated with the presence of columella tissues that are 3.8 times longer, 1.7 times wider, and 10.5 times more voluminous than the columellas of lateral roots. We propose that the lack of graviresponsiveness exhibited by lateral roots is due to the facts that they (i) produce smaller amounts of the inhibitor than primary (i.e., strongly graviresponsive) roots and (ii) are unable to redistribute the inhibitor so as to be able to create a concentration gradient sufficient to induce a pronounced gravitropic response.

scholarly journals Growth of Primary and Lateral Roots of Vicia faba L. in the Solution of Calcium Sulfate

2014 ◽  
Author(s):  
Michael Pyshnov
Keyword(s):  
Vicia Faba ◽  
Calcium Sulfate ◽  
Bacterial Contamination ◽  
Lateral Roots ◽  
Moist Air ◽  
Surface Sterilization ◽  
Primary Roots ◽  
Vicia Faba L ◽  
Healthy Growth ◽  
Primary And Lateral Roots

The presence of calcium sulfate in cultivating solution prevents bacterial contamination, browning/lignification and the death of the roots. There is no need for surface sterilization of seeds. No other ingredients, beside calcium sulfate, are needed for the healthy growth of the primary and lateral roots. There is no need to change the solution for several weeks when up to 60 lateral roots per seed can appear. A modification of the two-stage growing technique where the seeds are first suspended in moist air over the cultivating solution to grow primary roots, and then, the primary roots are covered with the cultivating solution to grow lateral roots, was used. The hypothesis is put forward that primary roots actually need water as a liquid to expel air, as the air is probably preventing the appearance of lateral roots.

scholarly journals IBA, Juvenility, and Position on Ortets Influence Propagation of Carolina Buckthorn from Softwood Cuttings

2002 ◽  
Vol 20 (1) ◽  
pp. 57-61 ◽  
Author(s):  
William R. Graves
Keyword(s):  
Dry Weight ◽  
Root Systems ◽  
Stem Cuttings ◽  
Indolebutyric Acid ◽  
High Quality ◽  
Influence Propagation ◽  
Primary Roots ◽  
Foliar Traits ◽  
Juvenile Plants

Abstract Carolina buckthorn (Rhamnus caroliniana Walt.) has horticultural potential but is not often propagated or grown as a nursery crop. My objective was to determine how three concentrations of indolebutyric acid (IBA) affect foliar quality of cuttings, callus development, and rooting of terminal and subtending softwood cuttings from both juvenile and mature stock plants (ortets). Twelve cuttings were assigned to each of 12 factorial treatment combinations and held under intermittent mist for five weeks. Fewer than 10% of cuttings not treated with IBA rooted. Rooting ≥75% resulted when IBA at 3 or 8 g/kg (3000 or 8000 ppm) was applied unless the cuttings were from terminal positions on mature ortets. High quality and dry weight of leaves were associated with subtending cuttings that were juvenile and with terminal cuttings that were mature, but these foliar traits did not lead to particularly high rooting percentages. Juvenile cuttings with IBA at 8 g/kg (8000 ppm) formed the most primary roots and developed root systems with the greatest weight, and dry weight of roots on juvenile cuttings that were subtending (25.6 mg) was about four times that ofjuvenile, terminal cuttings (6.5 mg). The longest individual roots were on juvenile cuttings with IBA at 3 or 8 g/kg (3000 or 8000 ppm). Frequency of callus on the cut end of stems decreased as IBA concentration increased and was 94 and 75%, respectively, for cuttings from juvenile and mature ortets. Nearly all cuttings with IBA at 3 or 8 g/kg (3000 or 8000 ppm) developed callus on wounds that had been made along the sides of their stems. The frequency of side callus was greater for juvenile, subtending cuttings than for juvenile, terminal cuttings; the opposite was true for mature cuttings. These results confirm the sensitivity of stem cuttings of Carolina buckthorn to IBA. The data also demonstrate that use of subtending wood will improve rooting percentages of cuttings from mature ortets, and that use of subtending cuttings from juvenile plants and of IBA at 8 g/kg (8000 ppm) will increase the number and dry weight of roots.

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