scholarly journals Genetic Diversity and Discrimination of Chimonanthus praecox (L.) Link Germplasm Using ISSR and RAPD Markers

HortScience ◽  
2007 ◽  
Vol 42 (5) ◽  
pp. 1144-1148 ◽  
Author(s):  
Kai-Ge Zhao ◽  
Ming-Qin Zhou ◽  
Long-Qing Chen ◽  
Donglin Zhang ◽  
Gituru Wahiti Robert

Chimonanthus praecox (wintersweet) is endemic to China. It has been cultivated there for more than 1000 years as a garden, potted, and cut-flower plant. Many cultivars have been developed during its long history of cultivation, and recently many germplasms were collected in Wuhan and Nanjing, China. The identification and genetic relationship of these resources were studied based mainly on morphological traits. In the current study, intersimple sequence repeat markers (ISSR) and random amplified polymorphic DNA markers (RAPD) were used for the first time to investigate 72 wintersweet clones from the two regions. Eleven ISSR primers amplified 115 bands, 90 (78.26%) of which were polymorphic. Nineteen RAPD primers amplified 165 bands, 105 (63.63%) of which were polymorphic. Either ISSR or RAPD markers were sufficient to distinguish all the clones surveyed. A Dendrogram based on Jaccard's similarity coefficients indicated that the distribution pattern of the 72 clones was coherent with their geographical origins. Most of the genetic variation (85.68% with ISSR data; 86.75% with RAPD data) occurred among clones within each region. However, the difference between Wuhan and Nanjing groups is statistically significant (ΦST = 0.143, P < 0.001, with ISSR data; ΦST = 0.132, P < 0.001, with RAPD data). Morphological variation and classification of wintersweet cultivars were also discussed compared with the genetic relationship based on ISSR and RAPD markers. This is the first report of the partitioning of genetic variability within and between different cultivated wintersweet regions, and it provides useful baseline data for optimizing sampling strategies in breeding. These results are important for future genetic improvement, identification, and conservation of Chimonanthus praecox germplasm.

Author(s):  
Lale Kabadayı

In the history of cinema, bad girl/boy characters are less common than other villain characters. However, these characters have a lot of influence on the audience. The Bad Seed movies, which are important book adaptations, are remarkable for the evil done by a charming, pretty little girl. The audience watched the story of this eight-year-old-girl for the first time with the adaptation made in 1956. The book was adapted as a television movie in the US both in 1985 and 2018. However, it was made in Turkey, too. This adaptation was shot in 1963 by director Nevzat Pesen. This black-and-white film is considered one of the best thriller-horror films of Turkish Cinema. In this study, the relationship of the little girl with evil will be examined in terms of differences in US and Turkish adaptations. Thus, the difference between the two cultures regarding the relationship between child and villainy will be evaluated from the point of cinema.


2011 ◽  
Vol 41 (No. 2) ◽  
pp. 73-78 ◽  
Author(s):  
L. Milella ◽  
J. Salava ◽  
G. Martelli ◽  
I. Greco ◽  
E.F. Cusimamani ◽  
...  

Random amplified polymorphic DNA (RAPD) markers are widely used for evaluating the genetic relationship of crop germplasm. Five different landraces of yacon (Smallantus sonchifolius (Poepp. and Hendl.) H. Robinson; Asteraceae) collected in various countries and showing different morphological traits were investigated using a total of 61 decamer primers. A total of 282 RAPD markers were scored and 28.7% of them were polymorphic at least within landraces. RAPD markers generated by one primer (OBP14) discriminated between all landraces. Markers were used to calculate genetic similarity coefficient and to build a dendrogram representing the genetic relationship between analysed landraces. The results suggest that RAPD markers could be used as a reliable tool to perform fingerprinting studies in Smallantus sonchifolius genome. This is the first report on the use of RAPDs to evaluate genetic distance and to distinguish between different landraces in yacon. &nbsp;


2016 ◽  
Vol 44 (2) ◽  
Author(s):  
Jiji George, K.T. Venkataramana, ◽  
P. Nainar M.K. Rajesh ◽  
Anitha Karun

The genetic relationship of 96 palmyrah palms, consisting of 24 indigenous accessions from Tamil Nadu and Andhra Pradesh, was investigated using Random Amplified Polymorphic DNA (RAPD) markers. Hundred and eighty primers were used initially to identify the polymorphic primers in six random samples and 10 polymorphic primers were selected to amplify the 96 palms. These 10 primers produced a total of 112 reproducible bands and out of them, 41 fragments (36.6 %) showed polymorphism.  The number of bands produced with each primer varied from seven to 15 with an average of 11.2 bands per primer.  The percent polymorphism ranged from 7.7 to 71.4 with an average of 37.4 per cent when all the primers were taken collectively.  UPGMA grouped all the accessions into two major clusters at 0.85 similarity value. The highest similarity value (0.96) was observed between the accessions KLKM-8 and THY-54 and the lowest similarity value (0.782) was obtained between ANBI-17 and RCML-11. The relatively low polymorphism suggests a narrow genetic diversity of palmyrah populations from which the present accessions have been derived and maintained over the years.


Author(s):  
Seha Akduman

Aim: In this retrospective crossectional study, it was aimed to evaluate the development of COPD in terms of urea, creatinine and some blood parameters in patients with allergic rhinitis. Method: Patients who were admitted to Kadıköy Medicana Hospital between 12.10.2017 and 12.10.2018 with respiratory complaints and has COPD diagnosis for the first time were evaluated retrospectively. Among the 845 patients admitted to the clinic for a one-year period, 160 patients with the diagnosis of COPD for the first time and 42 patients with a history of allergic rhinitis were subjected. The study group consisted of 63 patients with COPD who were diagnosed as COPD for the first time but without allergic rhinitis and 57 allergic rhinitis patients with no diagnosis of COPD. Findings: CRP, urea, WBC and neutrophil levels were higher in COPD group; creatinine and eisonofil levels were higher in COPD-Allergic rhinitis group and HGB was higher in the allergic rhinitis group. The differences between CRP and urea levels of COPD and allergic rhinitis group were statistically significant (p <0.05). The urea values were significantly different between COPD + allergic rhinitis and COPD groups (p <0.05). According to the results of ROC analysis, the value of urea for the COPD + allergic rhinitis group was statistically insignificant (p> 0.05), whereas it was statistically significant for the allergic rhinitis group (p <0.05). Conclusion: In detecting the difference between allergic rhinitis and COPD associated allergic rhinitis, urea levels may have a diagnostic value. An increase in urea in patients with allergic rhinitis may indicate comorbid COPD.


Author(s):  
Anna I. Reznichenko

The article is devoted to the literary and philosophical origins of Sergei Durylin’s report “On a Symbol in Dostoevsky” (the report was read in 1926 at a meeting of the Commission for the Study of Dostoevsky at the Literary Section of GAKhN). The history of the report in the context of the Literary Section is considered. Аbstracts and debates on the report are published for the first time. The relationship of Durylin’s ideas with the complex of Dostoevsky’s interpretations, developed by both the Symbolists (G.I. Chulkov) and Russian religious philosophers (P.A. Florensky, A.F. Losev) is shown. Both the report “On a Symbol in Dostoevsky’s” and the subsequent report “Landscape in Dostoevsky’s” are devoted to an anthropological and Christological story, connected with the symbolism of the setting sun, the symbolism of “oblique rays”, and its embodiment in Dostoevsky’s novels. Both texts are a continuation and a development of the same theme. A landscape is an artist’s mapping of nature, the created world; interiors are the artist’s image of the anthropomorphic world, the human space. The ontological symbol receives its sociocultural projection: a landscape or an interior. The problem of the relationship between the mapping/image and the object of the image, the problem of the ontological status of reality and its embodiment in the artistic/mythopoetic language, reflected in the report, corresponded to the focus of GAKhN on the development of a new “language of things” and a new concept of the humanitarian knowledge. The article is timed to coincide with the 200th anniversary of F.M. Dostoevsky, the 135th anniversary of S.N. Durylin, and the 100th anniversary of GAKhN.


2019 ◽  
pp. 100-113
Author(s):  
O.V. Shalygina

The article describes the original aesthetic and philosophical concept – the motor aesthetics of Akim Volynsky. Volynsky uses the concept of «motor aesthetics» in the Kniga likovanii, describing the value of circular lines for the «all aesthetics, visual, sound and motor», and particularly pirouette for motor aesthetics. The term «motor aesthetics of Akim Volynsky» is used in this article for the first time and is studied by the author from an interdisciplinary perspective. Motor aesthetics is developed by Volynsky for plastic art as a language of description of classical ballet, he introduces the basic concepts, formulates the laws, defines the basic philosophical categories that underlie it. The importance of Volynsky's work on the formation of the language of classical ballet description is recognized in the professional environment and theater criticism. The study of the motor aesthetics of Akim Volynsky is relevant in connection with the study of the philosophical foundations of intermedial analysis. The article deals with the problem of time and space in the motor aesthetics of Akim Volynsky for the first time. The direct connection of Volynsky's later works on ballet with his early article on Kant is revealed, the conclusion about the originality of Volynsky's philosophical position in relation to the categories of time and space is made. Using the thesaurus of Kant's transcendental aesthetics, Volynsky defines the two-act structural relationship of time and space according to the «par coupe» (fr) principle, which he regards as universal. It was concluded of Volynsky's motorial aesthetics value not only in the history of classical ballet and theatre criticism, the history of of the Russian literature and philosophy of the late 19th - early 20th century, but also in the modern philosophical anthropology and ontology.


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