scholarly journals OPTIMASI NAA DAN BAP TERHADAP PERTUMBUHAN DAN PERKEMBANGAN TUNAS MIKRO TANAMAN KANTONG SEMAR (Nepenthes mirabilis) SECARA IN VITRO

2015 ◽  
Vol 5 (2) ◽  
pp. 29
Author(s):  
ROSMAINA ROSMAINA ◽  
DINNI ARYANI
Keyword(s):  
In Vitro Culture ◽  
Research Design ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Alternative Method ◽  
Shoot Number ◽  
Root Explant ◽  
Induction Stage

Conventional propagation of Nepenthes was difficult to do. To overcome the problems were required alternative method such as in vitro propagation. The objective of this research was to obtain the best treatment of BAP + NAA on shoot multiplication of Nepenthes through in vitro culture. The research design used Randomized Completely Design consist of seven treatments, e.g. 1) ½ MS0 (control); 2) ½ MS + 1 ppm BAP + 0.5 ppm NAA; 3) ½ MS + 1 ppm BAP + 1 ppm NAA; 4) ½ MS + 1.5 ppm BAP + 0.5 ppm NAA; 5) ½ MS + 1.5 ppm BAP + 1 ppm NAA; 6) ½ MS + 2 ppm BAP + 0.5 ppm NAA dan 7) ½ MS + 2 ppm BAP + 1 ppm NAA. The parameter observed were number of shoot, number of nodul, number of leafs, number of pitcher and number of root. The result of this research showed that treatment of ½ MS + 1 ppm BAP + 1 ppm NAA is the best treatment compared to others. At induction stage, this treatment can produce the number of shoot, number of nodul, and number of root were 1.6 shoots/explant, 10.8 nodul/explant and 3.6 root/explant, respectively. At subculture, this treatment can produce the number of shoot, number of leafs, and number of pitcher were 5.8 shoots/explant, 12.4 leafs/explant and 5.2 pitcher/explant, respectively.

In vitro Propagation of Nothofagus obliqua (Fagaceae).

1995 ◽  
Vol 43 (6) ◽  
pp. 601 ◽  
Author(s):  
Pastur GJ Martinez ◽  
ME Arena
Keyword(s):  
In Vitro Propagation ◽  
Shoot Culture ◽  
Shoot Multiplication ◽  
Culture Conditions ◽  
Multiplication Rate ◽  
Nothofagus Obliqua ◽  
Low Salt ◽  
Broadleaved Tree ◽  
Induction Stage

Suitable culture conditions were investigated for in vitro propagation through shoot culture of seedlings and the acclimatization of the rooted plantlets of Nothofagus obliqua. Explant size and leaf expansion were critical for culture initiation. A medium with a low salt concentration and the addition of L-glutamine, as broadleaved tree medium, was suitable for shoot multiplication and it was influenced by both cytokinin and auxin. A multiplication rate of x5 has been obtained with 0.55 micro M benzyladenine (BA), and 0.09 micro M BA and 0.09 micro M IBA at day 63. Rooted shoots (51.9%) were obtained with 0.61 IBA and 1 week darkness at the induction stage. Rooted plants were then successfully acclimatised and 75% survival rate was obtained after 4 months.

scholarly journals Perbanyakan Mikro Colocasia esculenta (L.) Schott var. Antiquorum Melalui Penggunaan IAA

2018 ◽  
Vol 14 (1) ◽  
pp. 28-34
Author(s):  
Asnad E Louw ◽  
Henry Kesaulya ◽  
Imelda J Lawalata
Keyword(s):  
In Vitro Culture ◽  
Seed Quality ◽  
Block Design ◽  
Shoot Multiplication ◽  
Leaf Number ◽  
Growth Promoter ◽  
Shoot Height ◽  
Shoot Number ◽  
Micro Propagation

An appropriate in vitro propagation medium is necessary to improve the shoot multiplication ability and seed quality in micro propagation. In the Murashige Shoog in vitro culture medium, plant growth regulator can be added as growth promoter. This study aimed to determine the best IAA concentration for in vitro culture growth of Japanese taro (satoimo). The treatment consisted of 4 IAA concentrations, i.e. I0 (0 mg / l), I1 (0.5 mg/L), I2 (1 mg/L), I3 (1.5 mg/L) in randomized block design, with 5 replicates. The results of this study showed that IAA treatment gave an effect on the time of shoot emergence, shoot number, leaf number and root number of satoimo plantlet. IAA concentration of 0.5 mg/L was the best for satoimo shoot number, whereas 1 mg/L IAA was the best concentration for shoot number, shoot height and leaf number in micropropagation of satoimo. Keywords: IAA, in vitro culture, Japanese taro, micro propagation   ABSTRAK Media perbanyakan in vitrosangat diperlukan untuk meningkatkan kemampuan multipikasi tunas maupun kualitas bibit. Media Murashige Shoog (MS)dapatditambahkanzat pengatur tumbuh sebagai pemacu pertumbuhan dalam kultur in vitro.Penelitian ini bertujuan untuk mendapatkan konsentrasi IAA terbaik bagi pertumbuhan talas jepang dalam kulturin vitro. Perlakuan terdiri dari 4 taraf konsentrasi IAA yaitu I0 (0 mg/L), I1 (0,5 mg/L), I2 (1 mg/L), I3 (1,5 mg/L) yang diulang sebanyak 5 kali dalam rancangan acak kelompok. Hasil penelitian ini menunjukan bahwa pemberian IAA berpengaruh nyata terhadap saat muncul tunas, jumlah tunas, tinggi tunas, jumlah daun dan jumlah akar satoimo. Konsentrasi IAA 0,5 mg/L merupakan konsentrasi terbaik untuk pertumbuhan akar dan saat muncul tunas talas satoimo, sedangkan konsentrasi IAA 1 mg/L merupakan konsentrasi terbaik untuk jumlah tunas, tinggi tunas dan jumlah daun satoimo. Kata kunci: kultur in vitro, talas jepang, IAA, perbanyakan

scholarly journals In Vitro Propagation of Alexandrian Laurel (Danae racemosa L. Moench), a Valuable Ornamental Plant

HortScience ◽  
2013 ◽  
Vol 48 (10) ◽  
pp. 1301-1303
Author(s):  
Xiuli Shen ◽  
Guochen Yang ◽  
Zhongge (Cindy) Lu
Keyword(s):  
Morphological Variation ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Ornamental Plant ◽  
Efficient Production ◽  
Ms Medium ◽  
Culture Initiation ◽  
Shoot Number ◽  
Alternative Means

To overcome the limitations of traditional propagation, this research was initiated to develop an alternative means for efficient production of Alexandrian laurel (Danae racemosa L. Moench). An in vitro propagation protocol has been developed for Danae racemosa L. Moench using seeds as a source of material for culture initiation. Seedlings were produced after seeds were cultured for 3 month on MS (Murashige and Skoog, 1962) medium. Shoot multiplication occurred on MS medium with or without 6-benzylaminopurine (BAP) with 100% multiplication percentage. However, shoot number was significantly increased from an average of 2.8 to more than six with the addition of 5 or 25 μM BAP. Among two indole-3-butyric acid (IBA) treatments tested for rooting of seedlings, incorporation of 5 μM IBA in MS medium significantly increased rooting percentage to 86.4% compared with 71.2% without IBA. The greatest number of roots (three) was produced by 5-minute IBA pulse. However, both IBA treatments significantly reduced root length. The longest root (12.8 mm) was observed on MS medium without any IBA treatment and the shortest (6.1 mm) was produced by IBA pulse. In vitro-propagated plantlets grew well after transfer to a substrate of peat and pine bark (1:1) in the greenhouse. No morphological variation was observed.

In vitro propagation of the bay laurel (Laurus nobilis.L) in Morocco

2014 ◽  
Vol 4 (3) ◽  
pp. 96-103
Author(s):  
Abdelali Chourfi ◽  
Tajelmolk Alaoui ◽  
Ghizlane Echchgadda
Keyword(s):  
Seed Germination ◽  
In Vitro Culture ◽  
In Vitro Propagation ◽  
Aerial Part ◽  
Basal Medium ◽  
Axillary Buds ◽  
Laurus Nobilis

Laurus nobilis L. is among the species which are most threatened by massive degradation in Morocco. The multiplication by seed or by cuttings gives very low percentages of recovery that is insufficient to meet the demand of growing market. In vitro culture proves to be a tremendous asset to solve this problem. Our work has focused on the study of seed germination of this species and its multiplication from microcuttings. Finally, we studied the ac-climatization ability of the plantlets resulting from this germination. The study of the germination, via the further measurement of the length of the aerial part and the roots and the number of axillary buds for nine weeks, showed that the MS basal medium was more efficient than media 1/2M.S and WPM. Among the eight tested hormones, IAA yielded the best growth of the plantlets. Hormonal combination of NAA and kinetin resulted into a per-centage of the greatest success in reaching 67 % micropropagation. The study also revealed that the MS basal medium in the presence of the IAA plants can acclimate most easily in two types of substrates with improved development in the peat alone.

scholarly journals An efficient in vitro propagation protocol for snowdrop anemone (Anemone sylvestris L.) 

2017 ◽  
Vol 44 (No. 4) ◽  
pp. 186-194 ◽  
Author(s):  
Jana Šedivá ◽  
Pavla Zahumenická ◽  
Eloy Fernández Cusimamani
Keyword(s):  
Plant Growth ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Growth Characteristics ◽  
Root Induction ◽  
Shoot Induction ◽  
Free Medium ◽  
In Vitro Production ◽  
Vitro Production

This study investigated in vitro production of diploid (AS2) and tetraploid (AS4) cytotypes of snowdrop anemone. The effect of plant growth regulators (PGRs) on in vitro shoot multiplication and rooting was investigated. The effect of activated charcoal (AC) on root induction was also studied. Ploidy level affected growth characteristics during multiplication and rooting. Shoot induction in AS4 was higher on medium supplemented with cytokinin (3.2–3.6), while the AS2 clone formed the most shoots on PGR-free medium (3.6). The highest rooting percentage was achieved on PGR-free medium in both genotypes (AS2 clone, 100% and AS4 clone, 93.3%). The addition of AC to the PGR media largely increased root induction and root length. Rooted plantlets were successfully acclimatised in the greenhouse with 100% survival. Thus, the described micropropagation protocol represents a rapid and effective in vitro propagation method for utilisation in horticulture and conservation programmes of snowdrop anemone.

scholarly journals Micropropagation of Bacopa monnieri using Cyanobacterial Liquid Medium

1970 ◽  
Vol 20 (2) ◽  
pp. 225-231 ◽  
Author(s):  
Meenakshi Banerjee ◽  
Priyanka Modi
Keyword(s):  
Plant Tissue ◽  
In Vitro Propagation ◽  
Liquid Culture ◽  
Culture Media ◽  
Shoot Multiplication ◽  
Axillary Node ◽  
Bacopa Monnieri ◽  
Shoot Initiation ◽  
Aulosira Fertilissima

Hot extract of Aulosira fertilissima (cyanobacterium) added in different proportions to MS as a liquid culture media for the in vitro propagation of Bacopa monnieri (L.) Pennell. Maximum numbers of shoots were induced from axillary node in MS media (40 ml) + Aulosira extract (60 ml) and maximum shoot multiplication was observed when Kn (1.0 mg/l) was added in the shoot initiation media (mentioned above). Surprisingly rooting was also found to be best in the same combination of MS + cyanobacterial extract that was used for initiation and multiplication of shoots. On an average within a period of three subcultures (2 - 3 months) the nodal explants generated 400 shoots.  Rooted plantlets were successfully transferred to the field, after acclimation in the net house.   Key words: Baccopa monnieri, Cyanobacterial extract, Regeneration, Acclimation   D.O.I. 10.3329/ptcb.v20i2.6917   Plant Tissue Cult. & Biotech. 20(2): 225-231, 2010 (December)

scholarly journals Effect of Bavistin and Adenine Sulphate on In vitro Shoot Multiplication of Picrorhiza scrophulariiflora

1970 ◽  
Vol 19 (2) ◽  
pp. 237-245 ◽  
Author(s):  
Pranay Bantawa ◽  
Olivia Saha Roy ◽  
Parthadeb Ghosh ◽  
Tapan Kumar Mondal
Keyword(s):  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Multiple Shoots ◽  
Multiple Shoot ◽  
Virgin Soil ◽  
Adenine Sulphate ◽  
Multiple Shoot Buds ◽  
Picrorhiza Scrophulariiflora ◽  
Regenerated Plantlets

An alternative protocol for in vitro propagation of Picrorhiza scrophulariiflora is described using bavistin and adenine sulphate. The explants differentiated into multiple shoot buds on MS supplemented with various concentrations of bavistin and adenine sulphate ranging from 0 - 400 mg/l either alone or in combination. Maximum number of multiple shoots were obtained on MS containing the combination of bavistin (100 mg/l) and adenine sulphate (100 mg/l). In this combination as high as 28 shoots per explant was achieved and also vetrification of the cultures were not recorded. This study also demonstrates that the bavistin has stronger cytokinin-like activity than adenine sulphate. For instance, it was observed that bavistin alone in the concentration of 300 mg/l produced as high as 24 shoots per explant, however, adenine sulphate (100 mg/l) could produce a maximum of 18 shoots per explant. Moreover, higher or lower concentration did not improve the shoot multiplication. The microshoots were separated from the multiple shoots and transferred to MS containing various concentrations of auxins. Among them, NAA (1 mg/l) produced as high as 6 roots per explant. The regenerated plantlets were hardened in plastic cups (6 x 8 cm) containing 9 : 1 virgin soil and soil at Kyongnosla nursery and acclimated for four weeks. A 90% survival rate of the plants was recorded after 60 days. D.O.I. 10.3329/ptcb.v19i2.5441 Plant Tissue Cult. & Biotech. 19(2): 237-245, 2009 (December)

scholarly journals In Vitro Propagation of Viburnum treleasei Gand., an Azorean Endemic with High Ornamental Interest

HortScience ◽  
2009 ◽  
Vol 44 (6) ◽  
pp. 1668-1671 ◽  
Author(s):  
Mónica Moura ◽  
Maria Irene Candeias ◽  
Luís Silva
Keyword(s):  
In Vitro Propagation ◽  
Natural Populations ◽  
Shoot Multiplication ◽  
Shoot Development ◽  
Naphthaleneacetic Acid ◽  
Benzyl Adenine ◽  
Single Node ◽  
Surface Sterilization ◽  
Rare And Endangered

The purpose of our research was to establish a protocol for the in vitro culture of Viburnum treleasei, a rare and endangered taxon with high ornamental potential endemic to the Azores islands. The surface sterilization of the explants was better achieved with a pretreatment of 0.1% (w/v) Benomyl for 2 h followed by 0.2% (w/v) HgCl2 for 10 min with agitation. Shoot tips were the most efficient explants for shoot development and single-node segments for proliferation. Woody plant medium (WPM) was adequate for all micropropagation stages. For culture establishment and shoot development, a hormone-free medium was adequate, whereas a 1.1 μM N6-benzyl adenine medium supplement was more efficient for shoot multiplication. Elongation and rooting could be carried out on a 1.3 μM 1-naphthaleneacetic acid-supplemented medium. Acclimatization of in vitro-produced plantlets was achieved after 1 month with a success rate of 50%. This in vitro propagation procedure will be useful for the conservation of Viburnum treleasei through production of morphologically true-to-type plants, allowing the recovery of depleted natural populations. Chemical names used: N6-benzyl adenine (BA); 1-naphthaleneacetic acid (NAA); HgCl2 (mercury bichloride).

scholarly journals Effect of gelling agents on in vitro development of Amelanchier canadensis ‘Rainbow Pillar’

2013 ◽  
Vol 19 (3-4) ◽  
Author(s):  
A. Fira ◽  
K. Magyar-Tábori ◽  
I. Hudák ◽  
D. Clapa ◽  
J. Dobránszky
Keyword(s):  
Guar Gum ◽  
Growth Parameters ◽  
Shoot Multiplication ◽  
Wheat Starch ◽  
Gelling Agent ◽  
Shoot Cultures ◽  
Gelling Agents ◽  
Shoot Number ◽  
The Media

In vitro shoot multiplication responses of Amelanchier canadensis ‘Rainbow Pillar’ were studied on media solidifi ed with different gelling agents. The media were gelled either with 6.8 g l-1 fi brous agar-agar, or 50.0 g l-1 wheat starch, or 20.0 g l-1 Guar gum, or 15 g l-1 Isubgol or 50.0 g l-1 wheat starch mixed with 0.5 g l-1 Phytagel. Shoot cultures were grown for two months, thereafter the multiplication rates (number of newly developed shoots per explant) were counted and the length of shoots were measured. We found that the highest shoot multiplication of Amelanchier canadensis ‘Rainbow Pillar’ occurred on media gelled with Guar gum, while the longest shoots developed on media with Starch. About four-fold shoot number were obtained on media with Guar gum compared to the weakest results found on media gelled with Isubgol. Finally, considering all factors (shoot growth parameters, costs) the most economical gelling agent for Amelanchier canadensis ‘Rainbow Pillar’ was proved to be wheat starch among the tested alternatives which allows a 75.6% cost reduction.

scholarly journals Establishment and In Vitro Propagation of a Putative Variant of Periwinkle

2000 ◽  
Vol 5 (1) ◽  
pp. 15
Author(s):  
A. S. AI-Wasel
Keyword(s):  
Acetic Acid ◽  
In Vitro Propagation ◽  
Shoot Multiplication ◽  
Shoot Elongation ◽  
Nodal Segments ◽  
Naphthalene Acetic Acid ◽  
Murashige And Skoog Medium ◽  
Half Strength ◽  
Bud Breaking

Shoot multiplication of a putative variant of Catharanthus roseus (L.) G. Don, was achieved in vitro using shoot tips and nodal segments as explants. The addition of growth regulators to establishment medium stimulated bud breaking and shoot elongation. The maximum shoot multiplication (15.1 shoots/microshoot) and the longest shoots (7.0 cm) occurred on Murashige and Skoog medium (MS) containing 1.0 mg L-1 of N6-Benzyladenine (BA) and a- Naphthalene acetic acid (NAA). All microshoots formed roots and normal root morphology occurred on half strength MS salt supplied with 0.5 mg L-1 NAA or Indole-B-Butyric acid (IBA). Rooted microshoots (95 %) were successfully transferred to soil.

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