scholarly journals Genetic Diversity in Cynara cardunculus Determined by Sequence-related Amplified Polymorphism Markers

2007 ◽  
Vol 132 (2) ◽  
pp. 208-212 ◽  
Author(s):  
Vanina Cravero ◽  
Eugenia Martín ◽  
Enrique Cointry

Twenty-six wild and cultivated accessions of cardoon [Cynara cardunculus L. var. sylvestris (Lam.) Fiori and C. cardunculus var. cardunculus L., respectively] and globe artichoke [C. cardunculus var. scolymus (L.) Fiori] were analyzed to evaluate genetic diversity using sequence-related amplified polymorphism (SRAP) markers. Eleven qualitative and quantitative traits were evaluated and euclidean distances among genotypes were calculated. A total of 15 primer pairs were initially assayed and seven of them were finally selected as a result of their consistent amplification together with the clear banding patterns obtained. Genetic distances were calculated according to standardized Jaccard's distance index. Both matrices were subjected to cluster analysis. Dendrograms showed that cardoons were clearly separated from artichoke. These results showed that SRAP markers will be useful tools for studying genetic diversity in C. cardunculus.

Genetika ◽  
2015 ◽  
Vol 47 (2) ◽  
pp. 571-580
Author(s):  
Vladan Popovic ◽  
Aleksandar Lucic ◽  
Danijela Ristic ◽  
Ljubinko Rakonjac ◽  
Sabahudin Hadrovic ◽  
...  

The analysis of Bald cypress genetic variability at the level of test trees was performed using RAPD (Random Amlified Polymorphic DNA) markers. RAPD analysis was performed on 20 test trees with 13 primers. A total of ten primers gave a clear picture while three primers amplified weakly. 60 is a total number of detected bands obtained by RAPD analysis with 10 selected primers, and the average number of bands is 6. Based on presence/absence of RAPD fragments among all 20 Bald cypress test trees were calculated similarity coefficients by Dice and they range from 0.73 to 1. Based on similarity coefficients was performed the cluster analysis and results were presented as a dendrogram. All 20 test trees were grouped into two sub-clusters. Test trees 1, 4 and 11 were grouped in the first sub-cluster while other test trees were grouped in the second sub-cluster. By analysis of relations within every sub-cluster and sub-sub-cluster the existence of genetic distances between observed test trees can be noticed. The greatest similarity is between test trees 2, 12, 15 and 18. The results of genetic similarity and distance between observed test trees indicate the overwhelming presence of genetic diversity.


2015 ◽  
Vol 19 (2) ◽  
pp. 273-296
Author(s):  
R. M. Fahmy ◽  
Abeer A. H. Elhalwagi ◽  
Sahar F. M. El-Hefnawy

2011 ◽  
Vol 18 (2) ◽  
pp. 93-104 ◽  
Author(s):  
Fatemeh Mohammadzadeh ◽  
Hassan Monirifar ◽  
Jalal Saba ◽  
Mostafa Valizadeh ◽  
Ahmad Razban Haghighi ◽  
...  

Genetic diversity among and within 10 populations of Iranian alfalfa, from different areas of Azarbaijan, Iran was analyzed by screening DNA from seeds of individual plants and bulk samples. In individual study, 10 randomly amplified polymorphic DNA (RAPD) primers produced 156 polymorphic bands and a high level of genetic diversity was observed within populations. The averages of total and within population genetic diversity were 0.2349 and 0.1892, respectively. Results of analysis of molecular variance (AMOVA) showed the great genetic variation existed within populations (81.37%). These Results were in agreement with allogamous and polyploid nature of alfalfa. Cluster analysis was performed based on Nei’s genetic distances resulting in grouping into 3 clusters which could separate breeding population from other populations. Results of cluster analysis were in consistent with morphological and geographical patterns of populations. The results of bulk method were different from individual analysis. Our results showed that RAPD analysis is a suitable method to study genetic diversity and relationships among alfalfa populations.Keywords: Alfalfa; RAPD; Genetic diversity; Analysis of Molecular Variance; Cluster analysis.DOI: http://dx.doi.org/10.3329/bjpt.v18i2.9296Bangladesh J. Plant Taxon. 18: (2): 93-104, 2011 (December)


2017 ◽  
Vol 16 (1) ◽  
Author(s):  
L.P. de Carvalho ◽  
F.J.C. Farias ◽  
J.I.S. Rodrigues ◽  
N.D. Suassuna ◽  
P.E. Teodoro

2020 ◽  
pp. 202-212
Author(s):  
S.A. Veeresh ◽  
J.S. Minimol ◽  
B. Suma ◽  
P.S. Panchami ◽  
K.S. Shilpa

The current research work was carried out to evaluate the genetic diversity associated with thirty cocoa accessions resistant to Phytophthora. The cluster analysis and principal component analysis evaluated the genetic variability among the different genotypes. The highest number of genotypes were observed in cluster III (8) when qualitative traits were considered. In quantitative cluster analysis, most of the genotypes were placed in separate clusters due to high variability in the germplasm. Principal component (PC) analysis showed that the first three PCs with more than one Eigen-value contributed to 79.9 per cent of variability for different traits. When qualitative and quantitative characters were considered along with resistant reaction, clusters with genotypes highly resistant to Phytophthora pod rot were observed. Hybridization programme involving these resistant hybrids belonging to diverse clusters will result in high yielding hybrids with ample resistance.


2021 ◽  
Vol 26 (2) ◽  
pp. 2541-2547
Author(s):  
MIHNEA VLADIMIRESCU ◽  
◽  
IOANA CĂTĂLINA PAICA ◽  
CRISTIAN BANCIU ◽  
GABRIEL MIHAI MARIA ◽  
...  

Halimione verrucifera – an extreme halophyte plant species spread mainly in Asia with a few locations in South Eastern Europe – have the westernmost marginal populations in Romania. In order to asses population fitness and conservation status, genetic diversity among and within population was estimated using ISSR markers.A set of 6 primers were utilized after selection from a primer set which showed clear and reproducible banding patterns, generating a total number of 86 loci. A presence/absence matrix was generated by evaluating the gel electrophoresis banding patterns of PCR products. From this, genetic distances between individuals and populations were calculated, which were used in all subsequent generation of genetic diversity indices, assuming the Hardy-Weinberg equilibrium. Result revealed a low expected heterozygosity for both populations. Further testing by constructing an UPGMA dendrogram and a PCoA analysis confirmed that populations are clearly separated and with levels of genetic diversity that could ensure population survival in time and space.


2018 ◽  
Vol 71 (4) ◽  
Author(s):  
Saima Mir Arain ◽  
Mahboob Ali Sial ◽  
Karim Dino Jamali ◽  
Khalil Ahmed Laghari

Wheat is a leading cereal, playing a crucial role in feeding the hungry world and improving global food security. The present study was undertaken to comparatively analyze the extent of genetic diversity for various quantitative traits among the wheat material exotic to Pakistan, received from CIMMYT (The International Maize and Wheat Improvement Center), Mexico. Nineteen advanced lines from the Semi-Arid Wheat Yield Trial (SAWYT) were studied along with a local cultivar, considered a control (NIA-Amber). Data were recorded on nine important agro-morphic traits. The compared genotypes differed significantly (<em>p</em> ≤ 0.05) in the studied traits, where line V6 produced the highest mean grain yield (6,049 kg ha<sup>−1</sup>) and maximum 1,000-grain weight (45.0 g). Other lines, V19, V17, and V2, also showed superiority in yield (5,723, 5,150, and 5,067 kg ha<sup>−1</sup>, respectively). Days to heading established a significant positive association with days to maturity (<em>r</em> = 0.7995), plant height (<em>r</em> = 0.3168), spike length (<em>r</em> = 0.2696), and spikelets per spike (<em>r</em> = 0.4391). The important yield associated trait, 1,000-grain weight, had a highly significant positive correlation (<em>r</em> = 0.6833) with grain yield. Cluster analysis for various quantitative traits showed important information about genetic diversity for the studied traits among wheat genotypes. Hence, selection of genotypes for higher grain yield based on these traits could be useful for future breeding.


HortScience ◽  
2016 ◽  
Vol 51 (8) ◽  
pp. 972-979 ◽  
Author(s):  
Xinyi Zhang ◽  
Li Liao ◽  
Zhiyong Wang ◽  
Changjun Bai ◽  
Jianxiu Liu

Molecular genetic diversity and relationships among 86 Chrysopogon aciculatus (Retz.) Trin. accessions were assessed using intersimple sequence repeat (ISSR) and sequence-related amplified polymorphism (SRAP) markers. Twenty-five ISSR markers generated 283 amplification bands, of which 266 were polymorphic. In addition, 576 polymorphic bands were detected from 627 bands amplified using 30 SRAP primers. Both marker types revealed a high level of genetic diversity, with ISSR markers showing a higher proportion of polymorphic loci (PPL; 94%) than SRAP markers (91.87%). The ISSR and SRAP data were significantly correlated (r = 0.8023). Cluster analysis of the separate ISSR and SRAP data sets clustered the accessions into three groups, which generally were consistent with geographic provenance. Cluster analysis of the combined ISSR and SRAP data set revealed four major groups similar to those based solely on ISSR or SRAP markers. The findings demonstrate that ISSR and SRAP markers are reliable and effective tools for analysis of genetic diversity in C. aciculatus.


2018 ◽  
Vol 75 (2) ◽  
pp. 165 ◽  
Author(s):  
Kaluram ◽  
Jai Prakash ◽  
S. K. Singh ◽  
A. K. Goswami ◽  
Preeti Singh ◽  
...  

2017 ◽  
Vol 39 (1) ◽  
Author(s):  
JAMILE DA SILVA OLIVEIRA ◽  
FÁBIO GELAPE FALEIRO ◽  
NILTON TADEU VILELA JUNQUEIRA ◽  
MARCELO LIBINDO VIANA

ABSTRACT The aim of this study was to characterize Passiflora spp. accessions and its genetic diversity based on quantitative morphological descriptors of flowers and fruits. The study was conducted at Embrapa Cerrados, Planaltina-DF. Fifteen Passiflora spp. accessions were characterized using 14 quantitative morphological descriptors. Genetic distances among accessions were estimated based on Mahalanobis’ generalized distance. Cluster analysis via dendrogram and graphic dispersion was analyzed. The relative contribution of characters for accession divergence was also calculated. The morphoagronomic characterization based on quantitative descriptors of flowers and fruits contributed to the differentiation of Passiflora spp. accessions, serving as an important tool for variability quantification. This information is useful to perform Passiflora spp. characterization and genetic diversity studies.


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