scholarly journals Immunohistochemical Analysis of Expression of GATA3 in Carcinoma Breast and its Correlation with Prognostic Parameters

2021 ◽  
Vol 8 (2) ◽  
pp. A26-32
Author(s):  
Tanvi Suri ◽  
Charanjeet Ahluwalia
Keyword(s):  
Histological Grade ◽  
Statistical Significance ◽  
Tumour Size ◽  
Lymph Node Status ◽  
Inverse Association ◽  
Prognostic Parameters ◽  
Tumour Grade ◽  
Carcinoma Breast ◽  
Gata3 Expression ◽  
Her 2

Background: GATA3 plays an essential role in the normal development and function of the mammary gland where it promotes the luminal transcriptional program. Its loss is implicated in the pathogenesis of breast cancer. We proposed to study the expression of GATA3 in carcinoma breast by immunohistochemistry and determine its correlation with prognostic parameters. Methods: The expression pattern of GATA3 was evaluated by immunohistochemistry in 30 cases of invasive breast carcinoma. GATA3 scoring was done and a score of ≥ 1+ was considered positive. Patient characteristics, including age, tumour laterality, tumour size, lymph node status, tumour grade, histological type, molecular subtypes were collected. The relationships between protein expression and clinicopathological variables were analysed. Statistical significance was determined by Pearson’s chi-square test and Mann Whitney U test (for age). Result: 46.7% of cases (14/30) scored positive for GATA3 expression in tumour cells including 63% of luminal subtypes, 14% of Her-2 neu enriched carcinomas and 20% of triple negative carcinomas. Most positive cases (35.7%) demonstrated 3+ staining. GATA3 expression showed an inverse association with histological grade (P = 0.012) and HER-2 overexpression (P = 0.038), and a direct association with ER expression (P =0.017) and PR expression (P =0.009). Conclusion: GATA3 is luminal marker as it shows strong association with ER and PR in breast cancers. High GATA3 expression is also correlated good prognostic parameters like low tumour grade. Our findings advocate for GATA3 as a promising new breast-specific immunomarker.

Role of progesterone receptor status (PR) as predictive factor of pathological complete response (pCR) to neoadjuvant chemotherapy (NACT) in breast cancer (BC) patients (pts)

2009 ◽  
Vol 27 (15_suppl) ◽  
pp. 637-637
Author(s):  
J. M. Perez Garcia ◽  
C. Saura ◽  
E. Muñoz ◽  
G. Sanchez-Olle ◽  
P. Gomez ◽  
...  
Keyword(s):  
Predictive Factor ◽  
Multivariate Analyses ◽  
Histological Grade ◽  
Statistical Significance ◽  
Univariate Analysis ◽  
Complete Response ◽  
Her 2 ◽  
Few Data

637 Background: While estrogen receptor (ER) negativity has been consistently associated to higher pCR rates after NACT in BC, few data regarding the association of PR with pCR has been reported. PR positivity has been related to taxol resistance in in vitro chemosensitivity assays. Our aim was to investigate the putative role of PR in the prediction of pCR in a cohort of BC pts receiving NACT. Methods: Medical reports of all pts receiving NACT and breast surgery between 2004 and 2006 in our institution were reviewed. Baseline clinical and histological features, along with type of preoperative therapy were examined as variables for association with pCR (no invasive tumor in breast and axilla) using univariate and multivariate analyses. Results: 128 pts were included. 73.4% received anthracycline and taxanes (A&Tx) based CT and 9.4% also received trastuzumab (T). PR+ (≥ 10%) was significantly associated with low histological grade (HG), low Ki67 (<10%), HER-2- (Herceptest 0,1,or 2 with FISH-) and ER+ (≥10%). Overall pCR rate was 18.8%. No PR+ patient achieved pCR. In univariate analysis, high HG, ER-, PR-, HER-2-, high Ki67, T therapy, and number of CT cycles were significantly associated with pCR. In multivariate analysis only HER-2 and PR status were statistically significant. Similarly, PR and HER-2 status were independently related to pCR in the subgroup of pts receiving A&Tx (N = 94, 20.2%pCR). In the HER-2- subgroup (n = 89, 9%pCR) only ER independently predicted pCR, while in the HER-2+ subgroup (n = 33, 42.4%pCR, 57.1%pCR with T), both PR- and T therapy were the only predictive factors for pCR in the univariate and multivariate analyses. When the 24 HER-2+ pts treated with A&Tx (23 taxol/1 taxotere) ± T were separately analyzed, only PR retained statistical significance in the multivariate model. Conclusions: In our cohort of BC pts treated with NACT, PR status independently predicts pCR in the whole population as well as in the A&Tx-treated and in the HER-2+ subgroups. In the HER-2+ pts receiving A&Tx ± T PR negativity was the only factor associated with pCR. PR status merits further investigation as a predictive factor for pCR to NACT, in particular in HER-2+ population. No significant financial relationships to disclose.

scholarly journals Comparison of ER, PR, Ki67 and HER-2/neu Reactivity Pattern with Patients’ Age, Histologic Grade, Tumor Size and Lymph Node Status in Invasive Ductal Breast Cancer

2020 ◽  
Vol 13 (6) ◽  
Author(s):  
Mahsa Ahadi ◽  
Motahareh Heibatollahi ◽  
Sara Zahedifard
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Tumor Size ◽  
Histological Grade ◽  
Lymph Node Involvement ◽  
Lymph Node Status ◽  
Invasive Ductal Breast Cancer ◽  
Ductal Breast Cancer ◽  
Node Involvement ◽  
Her 2

Background: Breast cancer is the most prevalent neoplasm diagnosed in Iranian women. Objectives: The current study was performed to measure the hormone receptor status and its possible connection with the patient’s age, tumor size, histological grade, and lymph node status and involvement in patients with invasive ductal breast cancer (IDBC) Methods: A total of 103 women with IDBC recently diagnosed at the Department of Pathology of Shohada-E-Tajrish Hospital were entered into this study. The mean age of the patients was 48.4 years, and 59.2% of cases were 50 years old or less. Results: Most lesions (78.6%) were more than 2 cm at their greatest dimension. Grade-II lesions were observed in a large number of patients and 59.8% of cases had lymph node involvement. Positive ER, PR, and HER-2/neu were detected in 59%, 57%, and 29% of patients, respectively. A significant correlation was found between patients’ age and histologic score, tumor dimension and both histologic score and nuclear grade, and, finally, between lymph node involvement and nuclear grade. Conclusions: According to previous studies, the evaluation of hormone receptor status in patients with breast cancer is strongly recommended. Here, by studying its possible connection with the patient’s age, tumor size, histological grade, and lymph node metastasis, we detected some biomarkers, which could be used as prognostic indices in these patients. These biomarkers could help us in the clinical management of patients with IDBC by providing the best therapeutic options.

scholarly journals Significance of Immunohistochemical Biomarker in Breast Carcinoma from a Single Tertiary Care Hospital of Southern Assam

2021 ◽  
Author(s):  
Naiding Momota ◽  
Choudhury Biswadeep ◽  
Bezbaruah Babul ◽  
Nivedita Dasgupta ◽  
Deuri Biman ◽  
...  
Keyword(s):  
Lymph Node ◽  
Breast Carcinoma ◽  
Tumor Size ◽  
Tertiary Care Hospital ◽  
Histological Grade ◽  
Statistical Significance ◽  
Tertiary Care ◽  
Tumor Grade ◽  
Lymph Node Status ◽  
Care Hospital

Abstract Background: Breast carcinoma is the most common malignancy in female worldwide, leading cause of death in women.immunohistochemistry plays a very important role in the prognostication and treatment determination of breast carcinoma patients. Objective: To analyze the immunohistochemical markers in invasive carcinoma of breast and to correlate the expression of hormonal receptors with age of the patient, tumor size, histological grade and lymph node metastasis.Materials and method: The study was conducted on 88 infiltrating ductal breast carcinoma sample in a tertiary care hospital of Southern Assam for a period of two year (January2018- December 2019). Data including age, tumor size, and histologic grade and lymph node status retrieved from pathology department. Chi- square was used to determine the statistical significance between ER/PR status HER2/neu status along with their correlation with various clinicopathological parametres with respect to infiltrating ductal breast carcinoma.Result: The mean age of the patients was 56.6 years. We observed correlation between ER and PR expression with age, tumor size, tumor grade. There was correlation between HER2/neu expression and age only. None of the markers showed correlation with lymph node involvement (P>0.05).Conclusion: Our findings showed the importance of biomarkers (ER, PR, HER2/neu) expression as prognostic factors for therapeutic decision.

scholarly journals Association of HER-2 Expression with Age, Tumour Size, Grade, Differentiation and Lymph Node Deposit in Patients with Breast Cancer

2020 ◽  
Vol 32 (2) ◽  
pp. 14-21
Author(s):  
Sharmin Ferdousi ◽  
Shah Md Badruddoza ◽  
SM Asafuddullah
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Hormone Receptors ◽  
Tumour Size ◽  
Breast Cancer Incidence ◽  
Age Groups ◽  
Lymph Node Status ◽  
Cross Sectional ◽  
Size Grade ◽  
Her 2

Breast Cancer incidence is increasing, but its mortality has started to decrease. The existence of strong reliable prognostic and predictive factors is of utmost importance to the practicing clinician. Some factors are only prognostic such as age, tumor size, and lymph node status, while others are both prognostic and predictive such as hormone receptors and human epidermal receptor 2 (HER’s-2) status. This study was done to see the association of HER-2 positivity with age, tumour size, grade, differentiation and lymph node deposit in breast cancer patient. A cross-sectional type of descriptive study was conducted among 50 patients. Patient age range is from 22 to 64 years (mean 42.64 ± 10.26 years). Tumour size ranged from 1 to 8 cm (mean 4.39 ± 1.564cm). Well differentiated tumour was 20, moderately differentiated tumour was 18 cases and poorly differentiated was 12 cases. HER-2/neu negative included 35(70%) cases and positive included 15(30%) cases. In this study it was found that the association between age groups and tumour differentiation with HER-2/neu status was statistically significant (p= <0.05). TAJ 2019; 32(2): 14-21

Clinicopathological characteristics of oestrogen receptor negative, progesterone receptor positive breast cancers: re-evaluating subsets within this group

2016 ◽  
Vol 70 (4) ◽  
pp. 320-326 ◽  
Author(s):  
Syed Salahuddin Ahmed ◽  
Aye Aye Thike ◽  
Kathryn Zhang ◽  
Jeffrey Chun Tatt Lim ◽  
Puay Hoon Tan
Keyword(s):  
Ductal Carcinoma ◽  
Histological Grade ◽  
Tumour Size ◽  
Hormonal Treatment ◽  
Tissue Microarrays ◽  
Prognostic Indicator ◽  
Early Recurrence ◽  
Clinicopathological Parameters ◽  
Lymph Node Status ◽  
Breast Cancers

AimsThe presence of oestrogen and progesterone receptors (ER, PR) in breast carcinoma is an important prognostic indicator as well as a predictor of likely response to hormonal treatment. Current ambiguity surrounds ER-negative (–)/PR-positive (+) breast cancer (BC) as to whether this phenotype exists as a distinct entity. The independent predictive value of PR for treatment considerations is also in question, as some investigators believe ER status to be the single most important therapeutic predictive factor in BC. We undertook this study to determine the existence of ER(–)/PR(+) BC and the prognostic effect, if any, of this phenotype.MethodsWe investigated 267 archival documented ER(–)/PR(+) BCs diagnosed between January 1994 and July 2009. Histological slides were retrieved and reviewed. Tissue microarrays were constructed by selecting two 1 mm cores of tumour per case. Repeat immunohistochemistry was performed for confirmation of the ER(–)/PR(+) status. Clinicopathological parameters including age, ethnicity, tumour size, histological grade, histological subtype, associated ductal carcinoma in situ, lymphovascular invasion and lymph node status were evaluated.ResultsOn repeat immunohistochemistry, 92 tumours were confirmed as ER(–)/PR(+) BCs. This phenotype accounted for 1.1% of all BC phenotypes and exhibited different clinicopathological features and survival outcome when compared with other phenotypes. ER(–)/PR(+) tumours showed a trend for an early recurrence and poorer overall survival as compared with the patients with ER(+)/PR(+) tumours and similar to ER(–)/PR(–) tumours.ConclusionsOur findings suggest that ER(–)/PR(+) BCs exist, although rare, with distinct pathological and clinical characteristics from patients with ER(+)/PR(+) BCs.

scholarly journals HER-2/neu overexpression in invasive ductal breast cancer: An association with other prognostic and predictive factors

2007 ◽  
Vol 15 (1-2) ◽  
pp. 15-18 ◽  
Author(s):  
Tatjana Ivkovic-Kapicl ◽  
Slavica Knezevic-Usaj ◽  
Milana Panjkovic ◽  
Dragana Ðilas-Ivanovic ◽  
Mileta Golubovic
Keyword(s):  
Breast Cancer ◽  
Hormone Receptors ◽  
Histological Grade ◽  
Lymph Node Status ◽  
Breast Cancers ◽  
Protein Overexpression ◽  
Large Tumor ◽  
Invasive Ductal Breast Cancer ◽  
Ductal Breast Cancer ◽  
Her 2

Background: HER-2/neu is a proto-oncogene that is amplified/overexpressed in 15 to 30% of invasive breast cancers. The purpose of this study was to determine if any relationship exist between HER-2/neu protein overexpression and estrogen receptor (ER), progesterone receptor (PR), grade, size, and lymph node status in female breast cancer. Methods: A total of 100 cases of invasive ductal breast cancer were included in this study. The hormone receptors and HER-2/neu were studied immunohistochemically (IHC). Using the HER-2/neu DAKO scoring system, scores of 0, 1+ and 2+ were defined as negative and 3+ as positive. Results: HER-2/neu protein overexpression was seen in 20 (20%) of cases. HER-2/neu protein overexpression was present in 4 of 52 T1 lesions (8%), in 11 of 37 T2 lesions (30%), in 3 of 6 T3 lesions (50%), and in 2 of 5 T4 lesions (40%), (p<0.05). Protein overexpression was seen in 7 of 17 grade III tumors (41%), and 13 of 61 grade II tumors (21%). Overexpression was not detected in grade I tumors (p<0.01). Of the 20 Her-2/neu positive cases, ER- and PR-negative status was detected in 60% and 70%, respectively. Conclusion: Statistically significant correlation was found between HER-2/neu protein overexpression and large tumor size, high histological grade, and ER-, PR-negativity. There was no correlation with lymphonodal status.

scholarly journals Correlation of ER PR receptors with other prognostic parameters of breast carcinoma

2021 ◽  
Vol 8 (4) ◽  
pp. 437-442
Author(s):  
Vishrabdha Rahul Pawar ◽  
Nekta Anand ◽  
Shahnaz Zaidi
Keyword(s):  
Breast Carcinoma ◽  
Tumor Size ◽  
Histological Grade ◽  
Tumor Type ◽  
Age Group ◽  
Prognostic Parameters ◽  
Carcinoma Breast ◽  
Poor Predictor ◽  
Size Grade ◽  
Grade Ii

To study the MRM specimens for size, grade of the tumor, LVI and LN metastases and the ER/PR receptors of the tumor. To study the association of ER/PR status with the above mentioned prognostic parameters. This three-year study includes 72 histopathologically confirmed cases of carcinoma breast. The tumor type, grade, LNM, LVE were reported on H & E. The ER-PR study was done of all 72 cases. The tumor size, grade, LNM, LVE were correlated with receptor status. Out of 72 cases majority (53%) of cases were in 41-50 years of age group, and tumor size was between 2-4 cm in (54.16%) cases; majority were of grade II carcinoma (60%) of which 59% were ER-PR positive. Out of 46 cases with negative LNM and LVE, 30 cases (65%) were ER-PR positive. In our observation there is no association between higher histological grade and ER-PR status. No obvious correlation with tumor size was noted. But increase in tumor size could also be a poor predictor of ER-PR status. LNM, LVE is poor predictor of ER-PR status.

scholarly journals Estrogen Receptors, Progesterone Receptors and their Correlation with respect to HER-2/neu Status, Histological Grade, Size of Lesion, Lymph node Metastasis, Lymphovascular Involvement and Age in Breast Cancer patients in a hospital in north India

2016 ◽  
Vol 7 (3) ◽  
pp. 28-34 ◽  
Author(s):  
Mushood G. Nabi ◽  
Andleeb Ahangar ◽  
Subiya Kaneez
Keyword(s):  
Breast Cancer ◽  
Lymph Node ◽  
Lymph Node Metastasis ◽  
Tumor Size ◽  
Hormone Receptor ◽  
Histological Grade ◽  
Low Grade ◽  
Inverse Association ◽  
Node Metastasis ◽  
Her 2

Background: Breast cancer is the second most common cancer overall (1.4 million cases, 10.9%) and ranks 5th as cause of death (458,000. 6.1%).The present study was aimed to find the pattern of expression of ER, PR, HER-2/neu and to correlate ER, PR status with respect to various clinicopathlogical factors like HER-2/neu over expression, histological grade, tumor size, lymphnode metastasis, lymphovascular invasion and age of the patient.Materials and Methods: Data from 139 patients was collected and correlation of ER, PR status with various clinicopathological factors was analysed.Results: Mean age at diagnosis was 48.5 years, 56.8% cases being ?50years of age. Tumor size ranged from 1 to 12 cm. Most lesions (57.5%) were>2 to 5 cm in size. The predominant morphology was Infiltrating Duct carcinoma-NOS (90.6%). Majority of cases presented as grade II(46.7%) lesions and lymph node involvement was seen in 66.9%. 49.6% cases were ER+, 49.6% cases were PR+, and HER-2/neu was positive (3+) in 15.8% cases. Older patients were mostly ER+PR+. Low grade tumors were mostly ER+PR+ as compared to high grade tumors. HER-2/neu status revealed an inverse association with hormone receptor positivity. No correlation was found between hormone receptor positivity and lymph node metastasis. Lymphovascular involvement was noted more in hormone negative ER-PR- tumors.Conclusion:  ER and PR expression in our patients was found to be comparable to the studies done in Indian subcontinent, but lower than studies done in west, but triple negative breast cancer prevalence was relatively higher in our study (34.5%).Asian Journal of Medical Sciences Vol. 7(3) 2016 28-34

Sign in / Sign up

Export Citation Format

Share Document