Evaluation of In Vitro Antioxidant Characteristics of Corn Starch Bioactive Films Incorporated With Bunium Persicum and Zataria Multiflora Essential Oils

2017 ◽  
Author(s):  
Majid Aminzare ◽  
Elham Amiri ◽  
Zahra Abbasi ◽  
Hassan Hassanzad Azar ◽  
Mohammad Hashemi
2014 ◽  
Vol 54 (3) ◽  
pp. 299 ◽  
Author(s):  
M. Taghavi-Nezhad ◽  
D. Alipour ◽  
M. D. Flythe ◽  
P. Zamani ◽  
G. Khodakaramian

Gas (CO2 and CH4) and ammonia production in the rumen represent major sources of lost carbon and nitrogen, respectively. The essential oils of some plants have been shown to decrease gas and ammonia production by selectively inhibiting rumen microbes. Particularly, those of Zataria multiflora (ZEO; thymol 21%, carvacrol 32%) and Mentha spicata (SEO; carvone 55%) were evaluated in vitro as ruminant-feed additives. The experiments employed mixed rumen microbes and a hyper-ammonia-producing bacterium (HAP) isolated from the rumen of a Mehraban sheep. Both ZEO and SEO decreased in vitro fibre digestibility and also gas production by mixed rumen microbes that were fermenting a typical growing-lamb diet. ZEO decreased ammonia concentration in mixed culture of rumen microbes, but SEO exerted the opposite effect. A bacterial isolate (MT8) was obtained from the rumen of a Mehraban sheep, and the 16S rRNA gene sequence indicated that it was most closely related to Clostridium bifermentans. Isolate MT8 exhibited rapid ammonia production when peptides were the growth substrate, which indicated that MT8 was a HAP. Both oils inhibited the growth and ammonia production of isolate MT8. However, ZEO decreased ammonia production at lower doses, and to a greater degree, than did SEO. These results indicated that both essential oils could potentially be used to modulate rumen fermentation. The detrimental effects on fibre digestion could be problematic in high-forage diets, and this requires further investigation. Isolate MT8 is the first described HAP from the Mehraban sheep rumen. Results on ammonia production by isolate MT8 and mixed rumen microbes indicate differential mode of action of each oil on this parameter.


2020 ◽  
Author(s):  
Farzaneh Mirzaei ◽  
Roghayeh Norouzi ◽  
Abolghasem Siyadatpanah ◽  
Bibi Fatemeh Haghirosadat ◽  
Fatemeh Rezaei ◽  
...  

Abstract Background: Trichomonas vaginalis, a parasitic flagellated protozoan, is one of the main non-viral sexually-transmitted diseases worldwide. Treatment options for trichomoniasis are limited to nitroimidazole compounds. However, resistance to these drugs has been reported, which requires the development of new anti-Trichomonas agents that confer suitable efficacy and less toxicity.Methods: In the present work, we assessed the effectiveness of the liposomal system containing essential oils of Bunium persicum and Trachyspermum ammi against T. vaginalis in vitro. Liposomal vesicles were prepared with phosphatidylcholine (70%) and cholesterol (30%) using the thin-film method. The essential oils of B. persicum and T. ammi were loaded into the liposomes using the inactive loading method. Liposomal vesicles were made for two plants separately. Their physicochemical features were tested using Zeta-Sizer, AFM and SEM. The anti-Trichomonas activity was determined after 12 and 24 hours of parasite cultures in TYI-S-33 medium. Results: After 12 and 24 hours of administration, the IC50 of the B. persicum essential oil nano-liposomes induced 14.41 µg/mL and 45.19 µg/mL, respectively. The IC50 of T. ammi essential oil nano-liposomes induced 8.08 µg/mL and 25.81 µg/mL, respectively. Conclusions: These data suggested that nano-liposomes of the essential oils of B. persicum and T. ammi may be a promising alternative to current treatments for Trichomonas infection.


2017 ◽  
Vol 19 (1) ◽  
pp. 1-9 ◽  
Author(s):  
Majid Aminzare ◽  
Mohammad Hashemi ◽  
Hassan Hassanzadazar ◽  
Elham Amiri ◽  
Zahra Abbasi

2020 ◽  
Author(s):  
Farzaneh Mirzaei ◽  
Roghayeh Norouzi ◽  
Abolghasem Siyadatpanah ◽  
Bibi Fatemeh Haghirosadat ◽  
Fatemeh Rezaei ◽  
...  

Abstract Background: Trichomonas vaginalis, a parasitic flagellated protozoan, is one of the main non-viral sexually-transmitted diseases worldwide. Treatment options for trichomoniasis are limited to nitroimidazole compounds. However, resistance to these drugs has been reported, which requires the development of new anti-Trichomonas agents that confer suitable efficacy and less toxicity.Methods: In the present work, we assessed the effectiveness of the liposomal system containing essential oils of Bunium persicum and Trachyspermum ammi against T. vaginalis in vitro. Liposomal vesicles were prepared with phosphatidylcholine (70%) and cholesterol (30%) using the thin-film method. The essential oils of B. persicum and T. ammi were loaded into the liposomes using the inactive loading method. Liposomal vesicles were made for two plants separately. Their physicochemical features were tested using Zeta-Sizer, AFM and SEM. The anti-Trichomonas activity was determined after 12 and 24 hours of parasite cultures in TYI-S-33 medium. Results: After 12 and 24 hours of administration, the IC50 of the B. persicum essential oil nano-liposomes induced 14.41 µg/mL and 45.19 µg/mL, respectively. The IC50 of T. ammi essential oil nano-liposomes induced 8.08 µg/mL and 25.81 µg/mL, respectively. Conclusions: These data suggested that nano-liposomes of the essential oils of B. persicum and T. ammi may be a promising alternative to current treatments for Trichomonas infection.


2020 ◽  
Author(s):  
Farzaneh Mirzaei ◽  
Roghayeh Norouzi ◽  
Abolghasem Siyadatpanah ◽  
Bibi Fatemeh Haghirosadat ◽  
Fatemeh Rezaei ◽  
...  

Abstract Background: Trichomonas vaginalis, a parasitic flagellated protozoan, is one of the main non-viral sexually-transmitted diseases worldwide. Treatment options for trichomoniasis are limited to nitroimidazole compounds. However, resistance to these drugs has been reported, which requires the development of new anti-Trichomonas agents that confer suitable efficacy and less toxicity.Methods: In the present work, we assessed the effectiveness of the liposomal system containing essential oils of Bunium persicum and Trachyspermum ammi against T. vaginalis in vitro. Liposomal vesicles were prepared with phosphatidylcholine (70%) and cholesterol (30%) using the thin-film method. The essential oils of B. persicum and T. ammi were loaded into the liposomes using the inactive loading method. Liposomal vesicles were made for two plants separately. Their physicochemical features were tested using Zeta-Sizer, AFM and SEM. The anti-Trichomonas activity was determined after 12 and 24 hours of parasite cultures in TYI-S-33 medium.Results: After 12 and 24 hours of administration, the IC50 of the B. persicum essential oil nano-liposomes induced 14.41 µg/mL and 45.19 µg/mL, respectively. The IC50 of T. ammi essential oil nano-liposomes induced 8.08 µg/mL and 25.81 µg/mL, respectively.Conclusions: These data suggested that nano-liposomes of the essential oils of B. persicum and T. ammi may be a promising alternative to current treatments for Trichomonas infection.


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