Essential oils, chemical constituents, antioxidant, antibacterial and in vitro cytotoxic activity of different Thymus species and Zataria multiflora collected from Iran

Pelargonium graveolens (Geranium) is a source of the finest quality of fragrance and its essential oils are used as antibacterial, and antifungal agents. The aim of the current research is to determine chemical constituents in the essential oil of P. graveolens by GC-MS and evaluate its antidiabetic activity via α-glucosidase inhibition assay. The chemical composition of P. graveolens essential oil was determined by GC/MS and its antidiabetic activity was assessed through inhibition of α-glucosidase enzyme in in-vitro models. GC-MS analysis determines 36 chemical components in the essential oil of P. graveolens leaves, and citronellyl isovalerate (10.41 %), menthol (9.61 %), linalool (8.63 %), p-menthone (6.31 %), and geranyl tiglate (4.99 %) were recorded as major constituents. The essential oil of P. graveolens leaves showed concentration dependant inhibition of α-glucosidase enzyme ranging from 28.13±1.41 to 74.24±2.53 µg/mL for concentration ranging from 31.25 to 1000 µg/mL. The IC50 values for of P. graveolens and acarbose were found as 93.72±4.76 and 80.4±2.17 µg/mL, respectively against the α-glucosidase enzyme. The study finding explores the chemical components of P. graveolens growing in the Iraqi Kurdistan region and scientifically supported its possible use in diabetic patients for controlling postprandial hyperglycemia.

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In VitroCytotoxic Potential of Essential Oils ofEucalyptus benthamiiand Its Related Terpenes on Tumor Cell Lines

Evidence-based Complementary and Alternative Medicine ◽

10.1155/2012/342652 ◽

2012 ◽

Vol 2012 ◽

pp. 1-8 ◽

Cited By ~ 32

Author(s):

Patrícia Mathias Döll-Boscardin ◽

Adilson Sartoratto ◽

Beatriz Helena Lameiro de Noronha Sales Maia ◽

Josiane Padilha de Paula ◽

Tomoe Nakashima ◽

...

Keyword(s):

Tumor Cell ◽

Essential Oils ◽

Cytotoxic Activity ◽

Cell Lines ◽

Cytotoxic Effect ◽

Herbal Remedy ◽

Jurkat Cells ◽

Tumor Cell Lines ◽

Hela Cell Lines

EucalyptusL. is traditionally used for many medicinal purposes. In particular, someEucalyptusspecies have currently shown cytotoxic properties. Local Brazilian communities have used leaves ofE. benthamiias a herbal remedy for various diseases, including cancer. Considering the lack of available data for supporting this cytotoxic effect, the goal of this paper was to study thein vitrocytotoxic potential of the essential oils from young and adult leaves ofE. benthamiiand some related terpenes (α-pinene, terpinen-4-ol, andγ-terpinene) on Jurkat, J774A.1 and HeLa cells lines. Regarding the cytotoxic activity based on MTT assay, the essential oils showed improved results thanα-pinene andγ-terpinene, particularly for Jurkat and HeLa cell lines. Terpinen-4-ol revealed a cytotoxic effect against Jurkat cells similar to that observed for volatile oils. The results of LDH activity indicated that cytotoxic activity of samples against Jurkat cells probably involved cell death by apoptosis. The decrease of cell DNA content was demonstrated due to inhibition of Jurkat cells proliferation by samples as a result of cytotoxicity. In general, the essential oils from young and adult leaves ofE. benthamiipresented cytotoxicity against the investigated tumor cell lines which confirms their antitumor potential.

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The effect of essential oils of Zataria multiflora and Mentha spicata on the in vitro rumen fermentation, and growth and deaminative activity of amino acid-fermenting bacteria isolated from Mehraban sheep

Animal Production Science ◽

10.1071/an12244 ◽

2014 ◽

Vol 54 (3) ◽

pp. 299 ◽

Cited By ~ 2

Author(s):

M. Taghavi-Nezhad ◽

D. Alipour ◽

M. D. Flythe ◽

P. Zamani ◽

G. Khodakaramian

Keyword(s):

Essential Oils ◽

Rumen Fermentation ◽

Gas Production ◽

Feed Additives ◽

Rrna Gene ◽

Ammonia Production ◽

Mentha Spicata ◽

Zataria Multiflora ◽

Rumen Microbes

Gas (CO2 and CH4) and ammonia production in the rumen represent major sources of lost carbon and nitrogen, respectively. The essential oils of some plants have been shown to decrease gas and ammonia production by selectively inhibiting rumen microbes. Particularly, those of Zataria multiflora (ZEO; thymol 21%, carvacrol 32%) and Mentha spicata (SEO; carvone 55%) were evaluated in vitro as ruminant-feed additives. The experiments employed mixed rumen microbes and a hyper-ammonia-producing bacterium (HAP) isolated from the rumen of a Mehraban sheep. Both ZEO and SEO decreased in vitro fibre digestibility and also gas production by mixed rumen microbes that were fermenting a typical growing-lamb diet. ZEO decreased ammonia concentration in mixed culture of rumen microbes, but SEO exerted the opposite effect. A bacterial isolate (MT8) was obtained from the rumen of a Mehraban sheep, and the 16S rRNA gene sequence indicated that it was most closely related to Clostridium bifermentans. Isolate MT8 exhibited rapid ammonia production when peptides were the growth substrate, which indicated that MT8 was a HAP. Both oils inhibited the growth and ammonia production of isolate MT8. However, ZEO decreased ammonia production at lower doses, and to a greater degree, than did SEO. These results indicated that both essential oils could potentially be used to modulate rumen fermentation. The detrimental effects on fibre digestion could be problematic in high-forage diets, and this requires further investigation. Isolate MT8 is the first described HAP from the Mehraban sheep rumen. Results on ammonia production by isolate MT8 and mixed rumen microbes indicate differential mode of action of each oil on this parameter.

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Effect of essential oils of Zataria multiflora on in vitro rumen fermentation, protozoal population, growth and enzyme activity of anaerobic fungus isolated from Mehraban sheep

Animal Feed Science and Technology ◽

10.1016/j.anifeedsci.2011.11.011 ◽

2012 ◽

Vol 172 (3-4) ◽

pp. 115-124 ◽

Cited By ~ 6

Author(s):

R. Talebzadeh ◽

D. Alipour ◽

M.J. Saharkhiz ◽

A. Azarfar ◽

M. Malecky

Keyword(s):

Enzyme Activity ◽

Population Growth ◽

Essential Oils ◽

Rumen Fermentation ◽

Anaerobic Fungus ◽

Zataria Multiflora ◽

In Vitro Rumen Fermentation

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Chemical Composition and In vitro Cytotoxic Activity of the Essential Oils of Stachys rupestris and Salvia heldreichiana, Two Endemic Plants of Turkey

Natural Product Communications ◽

10.1177/1934578x1300801134 ◽

2013 ◽

Vol 8 (11) ◽

pp. 1934578X1300801 ◽

Cited By ~ 1

Author(s):

Elif Ayşe Erdogan ◽

Ayşe Everest ◽

Laura De Martino ◽

Emilia Mancini ◽

Michela Festa ◽

...

Keyword(s):

Chemical Composition ◽

Essential Oils ◽

Cytotoxic Activity ◽

Cell Lines ◽

Cancer Cell ◽

Human Cancer ◽

Cancer Cell Lines ◽

Endemic Plants ◽

Total Oil

The chemical composition of the essential oils of two endemic plants of Turkey, Stachys rupestris Montbret et Aucher ex Benth. and Salvia heldreichiana Boiss. ex Benth., were obtained by hydrodistillation and studied by GC and GC–MS. In all, 46 compounds were identified, 22 for S. rupestris accounting for 94.6 % of the total oil and 30 for S. heldreichiana, accounting for 91.9 % of the total oil. The presence of diterpenoids (50.7%) characterized the oil from S. rupestris, while S. heldreichiana oil was rich in oxygenated sesquiterpenes (78.9%). The essential oils were evaluated for their in vitro potential cytotoxic activity on three human cancer cell lines. The oil of S. rupestris showed the higher antiproliferative activity against PC-3 and MCF-7 cancer cell lines.

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Chemical Composition of Nardostachys grandiflora Rhizome Oil from Nepal – A Contribution to the Chemotaxonomy and Bioactivity of Nardostachys

Natural Product Communications ◽

10.1177/1934578x1501000668 ◽

2015 ◽

Vol 10 (6) ◽

pp. 1934578X1501000 ◽

Cited By ~ 3

Author(s):

Prabodh Satyal ◽

Bhuwan K. Chhetri ◽

Noura S. Dosoky ◽

Ambika Poudel ◽

William N. Setzer

Keyword(s):

Escherichia Coli ◽

Antimicrobial Activity ◽

Candida Albicans ◽

Chemical Composition ◽

Essential Oil ◽

Essential Oils ◽

Cytotoxic Activity ◽

Biological Activities ◽

Mcf 7

The essential oil from the dried rhizome of Nardostachys grandiflora, collected from Jaljale, Nepal, was obtained in 1.4% yield, and a total of 72 compounds were identified constituting 93.8% of the essential oil. The rhizome essential oil of N. grandiflora was mostly composed of calarene (9.4%), valerena-4,7(11)-diene (7.1%), nardol A (6.0%), 1(10)-aristolen-9-ol (11.6%), jatamansone (7.9%), valeranal (5.6%), and cis-valerinic acid (5.7%). The chemical composition of N. grandiflora rhizome oil from Nepal is qualitatively very different than those from Indian, Chinese, and Pakistani Nardostachys essential oils. In this study we have evaluated the chemical composition and biological activities of N. grandiflora from Nepal. Additionally, 1(10)-aristolen-9-ol was isolated and the structure determined by NMR, and represents the first report of this compound from N. grandiflora. N. grandiflora rhizome oil showed in-vitro antimicrobial activity against Bacillus cereus, Escherichia coli, and Candida albicans (MIC = 156 μg/mL), as well as in-vitro cytotoxic activity on MCF-7 cells.

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Trypanocidal Essential Oils: A Review

Molecules ◽

10.3390/molecules25194568 ◽

2020 ◽

Vol 25 (19) ◽

pp. 4568 ◽

Cited By ~ 1

Author(s):

Mayara Castro de Morais ◽

Jucieudo Virgulino de Souza ◽

Carlos da Silva Maia Bezerra Filho ◽

Silvio Santana Dolabella ◽

Damião Pergentino de Sousa

Keyword(s):

Experimental Data ◽

Natural Products ◽

Essential Oils ◽

Therapeutic Potential ◽

Chemical Constituents ◽

Mechanisms Of Action ◽

New Drugs ◽

Important Group

Trypanosomiases are diseases caused by parasitic protozoan trypanosomes of the genus Trypanosoma. In humans, this includes Chagas disease and African trypanosomiasis. There are few therapeutic options, and there is low efficacy to clinical treatment. Therefore, the search for new drugs for the trypanosomiasis is urgent. This review describes studies of the trypanocidal properties of essential oils, an important group of natural products widely found in several tropical countries. Seventy-seven plants were selected from literature for the trypanocidal activity of their essential oils. The main chemical constituents and mechanisms of action are also discussed. In vitro and in vivo experimental data show the therapeutic potential of these natural products for the treatment of infections caused by species of Trypanosoma.

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