Gonadotropin Surge-Induced Expression of Progesterone Receptor Serves the Ovary as a Trigger of Ovulation and a Terminator of Ovulatory Inflammation

2019 ◽  
Author(s):  
Chan Jin Park ◽  
Po-Ching Lin ◽  
Radwa Barakat ◽  
Sherry Zhou ◽  
Jeong Moon Choi ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Induced Expression ◽  
Gonadotropin Surge

scholarly journals Gonadotropin-Induced Expression of Messenger Ribonucleic Acid for Cyclooxygenase-2 and Production of Prostaglandins E and F2α in Bovine Preovulatory Follicles Are Regulated by the Progesterone Receptor

Endocrinology ◽  
2006 ◽  
Vol 147 (10) ◽  
pp. 4713-4722 ◽  
Author(s):  
P. J. Bridges ◽  
C. M. Komar ◽  
J. E. Fortune
Keyword(s):  
Granulosa Cells ◽  
Medroxyprogesterone Acetate ◽  
Cyclooxygenase 2 ◽  
Induced Expression ◽  
Messenger Ribonucleic Acid ◽  
Preovulatory Follicles ◽  
Gonadotropin Surge ◽  
Induced Changes

Follicular production of prostaglandins (PGs) is essential for ovulation, but the factors mediating gonadotropin-induced secretion of PGE and PGF2α remain largely unknown. We tested the hypothesis that gonadotropin-induced changes in progesterone and its receptor (PR) mediate the increase in periovulatory PGs. Heifers were treated with PGF2α and GnRH to induce luteolysis and the LH/FSH surge (ovulation occurs ∼30 h after GnRH). Because there are two increases in intrafollicular progesterone/PR mRNA during the bovine periovulatory period, we first examined the temporal pattern of PG production by follicles collected at 0, 3.5, 6, 12, 18, and 24 h after GnRH. Although PGs did not increase in the follicular fluid until 24 h after GnRH, acute secretion of PGs by follicle wall (theca + granulosa cells) was initiated by 18 h and had increased manyfold by 24 h after GnRH. In vitro, FSH and LH induced dramatic transient increases in PG production by follicle wall and granulosa, but not theca, cells isolated from preovulatory follicles (0 h after GnRH). PG accumulation peaked on d 2 of culture, mimicking the secretion pattern after a gonadotropin surge in vivo. In cultures of follicle wall and granulosa cells, the PR antagonist mifepristone (MIFE, 1 μm) inhibited LH-induced PG secretion and the progestin medroxyprogesterone acetate (1 or 10 μm), but not the glucocorticoid dexamethasone (1 or 10 μm), overcame the effect of MIFE on PGs. Semiquantitative RT-PCR revealed that MIFE inhibited LH-induced expression of cyclooxygenase-2 mRNA in granulosa cells in vitro. Again, treatment with medroxyprogesterone acetate overcame the effect of MIFE. Together these results provide strong evidence that periovulatory increases in cyclooxygenase-2 mRNA, PGE, and PGF2α are mediated by gonadotropin-induced increases in progesterone/PR, indicating that in some species there is an important functional relationship between these pathways in the ovulatory cascade.

scholarly journals Progesterone-induced activation of membrane-bound progesterone receptors in murine macrophage cells

2014 ◽  
Vol 224 (2) ◽  
pp. 183-194 ◽  
Author(s):  
Jing Lu ◽  
Joshua Reese ◽  
Ying Zhou ◽  
Emmet Hirsch
Keyword(s):  
Progesterone Receptor ◽  
Inflammatory Responses ◽  
Progesterone Receptors ◽  
Murine Macrophage ◽  
Induced Expression ◽  
Receptor Alpha ◽  
Downstream Target ◽  
Macrophage Cells ◽  
Membrane Progesterone Receptor ◽  
Membrane Progesterone Receptor Alpha

Parturition is an inflammatory process mediated to a significant extent by macrophages. Progesterone (P4) maintains uterine quiescence in pregnancy, and a proposed functional withdrawal of P4 classically regulated by nuclear progesterone receptors (nPRs) leads to labor. P4 can affect the functions of macrophages despite the reported lack of expression of nPRs in these immune cells. Therefore, in this study we investigated the effects of the activation of the putative membrane-associated PR on the function of macrophages (a key cell for parturition) and discuss the implications of these findings for pregnancy and parturition. In murine macrophage cells (RAW 264.7), activation of mPRs by P4 modified to be active only extracellularly by conjugation to BSA (P4BSA, 1.0×10−7 mol/l) caused a pro-inflammatory shift in the mRNA expression profile, with significant upregulation of the expression of cyclooxygenase 2 (COX2 (Ptgs2)), Il1B, and Tnf and downregulation of membrane progesterone receptor alpha (Paqr7) and oxytocin receptor (Oxtr). Pretreatment with PD98059, a MEK1/2 inhibitor, significantly reduced P4BSA-induced expression of mRNA of Il1B, Tnf, and Ptgs2. Inhibition of protein kinase A (PKA) by H89 blocked P4BSA-induced expression of Il1B and Tnf mRNA. P4BSA induced rapid phosphorylation of MEK1/2 and CREB (a downstream target of PKA). This phosphorylation was inhibited by pretreatment with PD98059 and H89, respectively, revealing that MEK1/2 and PKA are two of the components involved in mPR signaling. Taken together, these results indicate that changes in membrane progesterone receptor alpha expression and signaling in macrophages are associated with the inflammatory responses; and that these changes might contribute to the functional withdrawal of P4 related to labor.

scholarly journals Ovarian Gonadotropin Surge Attenuating Factor (GnSAF) from Superovulated Women Inhibits GnRH Self-Priming in an Estrous Cycle Stage-Dependent Manner in the Rat: Involvement of Gonadotrope Progesterone Receptor (PR) Expression and Action.

2009 ◽  
Vol 81 (Suppl_1) ◽  
pp. 590-590
Author(s):  
Jose E. Sanchez-Criado ◽  
Ana Gordon ◽  
Rafaela Aguilar ◽  
Jose C. Garrido-Gracia ◽  
Juan A. Garcia-Velasco ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Estrous Cycle ◽  
Dependent Manner ◽  
Gonadotropin Surge ◽  
Estrous Cycle Stage

scholarly journals Gonadotropin Surge Induces Two Separate Increases in Messenger RNA for Progesterone Receptor in Bovine Preovulatory Follicles1

2002 ◽  
Vol 67 (6) ◽  
pp. 1981-1988 ◽  
Author(s):  
M. Jo ◽  
C.M. Komar ◽  
J.E. Fortune
Keyword(s):  
Progesterone Receptor ◽  
Messenger Rna ◽  
Gonadotropin Surge

Characterization of the functional progesterone receptor in an endometrial adenocarcinoma cell line (Ishikawa): Progesterone-induced expression of the α1 integrin

1996 ◽  
Vol 59 (1) ◽  
pp. 31-39 ◽  
Author(s):  
Bruce A. Lessey ◽  
Abiodun O. Ilesanmi ◽  
Arthur J. Castelbaum ◽  
Lingwen Yuan ◽  
Steven G. Somkuti ◽  
...  
Keyword(s):  
Progesterone Receptor ◽  
Cell Line ◽  
Endometrial Adenocarcinoma ◽  
Adenocarcinoma Cell Line ◽  
Induced Expression ◽  
Adenocarcinoma Cell

Deoxycholic (DOC) acid-induced expression of cyclooxygenase-2 (COX-2) in human colonic fibroblasts

2001 ◽  
Vol 120 (5) ◽  
pp. A170-A170
Author(s):  
S RAFIQ ◽  
Y ZHU ◽  
P LANCE
Keyword(s):  
Cyclooxygenase 2 ◽  
Induced Expression ◽  
Cox 2

1609: Sildenafil Does Not Reverse Hyperglycemia-Induced Expression of the Low Voltage-Activated T-Type (Alpha-1g) Calcium Channel that is Responsible for Down-Regulation of the Nitric Oxide-Cgmp Pathway

2004 ◽  
Vol 171 (4S) ◽  
pp. 425-425
Author(s):  
Suresh Sikka ◽  
Xiangbin Zeng ◽  
Anshiya Ramanitharan ◽  
Ming Li ◽  
Wayne J.G. Hellstrom
Keyword(s):  
Nitric Oxide ◽  
Calcium Channel ◽  
Low Voltage ◽  
Down Regulation ◽  
Induced Expression ◽  
Cgmp Pathway
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