Direct Comparison of Angiogenesis in Natural and Synthetic Biomaterials Reveals Matrix Porosity Regulates Endothelial Cell Invasion Speed and Sprout Diameter

2021 ◽  
Author(s):  
William Wang ◽  
Robert N. Kent III ◽  
Stephanie A. Huang ◽  
Evan H. Jarman ◽  
Eve H. Shikanov ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Invasion ◽  
Invasion Speed ◽  
Matrix Porosity ◽  
Natural And Synthetic

Direct comparison of angiogenesis in natural and synthetic biomaterials reveals that matrix porosity regulates endothelial cell invasion speed and sprout diameter

2021 ◽  
Author(s):  
William Y. Wang ◽  
Robert N. Kent ◽  
Stephanie A. Huang ◽  
Evan H. Jarman ◽  
Eve H. Shikanov ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Invasion ◽  
Invasion Speed ◽  
Matrix Porosity ◽  
Natural And Synthetic

Intersection of regulatory pathways controlling hemostasis and hemochorial placentation

2021 ◽  
Vol 118 (50) ◽  
pp. e2111267118
Author(s):  
Masanaga Muto ◽  
Damayanti Chakraborty ◽  
Kaela M. Varberg ◽  
Ayelen Moreno-Irusta ◽  
Khursheed Iqbal ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Cell Differentiation ◽  
Cell Invasion ◽  
Cell Development ◽  
Trophoblast Cell ◽  
Extravillous Trophoblast ◽  
Cell Phenotype ◽  
Trophoblast Cells ◽  
Rat Models ◽  
Endovascular Trophoblast

Hemochorial placentation is characterized by the development of trophoblast cells specialized to interact with the uterine vascular bed. We utilized trophoblast stem (TS) cell and mutant rat models to investigate regulatory mechanisms controlling trophoblast cell development. TS cell differentiation was characterized by acquisition of transcript signatures indicative of an endothelial cell-like phenotype, which was highlighted by the expression of anticoagulation factors including tissue factor pathway inhibitor (TFPI). TFPI localized to invasive endovascular trophoblast cells of the rat placentation site. Disruption of TFPI in rat TS cells interfered with development of the endothelial cell-like endovascular trophoblast cell phenotype. Similarly, TFPI was expressed in human invasive/extravillous trophoblast (EVT) cells situated within first-trimester human placental tissues and following differentiation of human TS cells. TFPI was required for human TS cell differentiation to EVT cells. We next investigated the physiological relevance of TFPI at the placentation site. Genome-edited global TFPI loss-of-function rat models revealed critical roles for TFPI in embryonic development, resulting in homogeneous midgestation lethality prohibiting analysis of the role of TFPI as a regulator of the late-gestation wave of intrauterine trophoblast cell invasion. In vivo trophoblast-specific TFPI knockdown was compatible with pregnancy but had profound effects at the uterine–placental interface, including restriction of the depth of intrauterine trophoblast cell invasion while leading to the accumulation of natural killer cells and increased fibrin deposition. Collectively, the experimentation implicates TFPI as a conserved regulator of invasive/EVT cell development, uterine spiral artery remodeling, and hemostasis at the maternal–fetal interface.

Effects of Fluid Shear Stress on Endothelial Cell Invasion Into Three-Dimensional Matrices

2007 ◽  
Author(s):  
Hojin Kang ◽  
Kayla J. Bayless ◽  
Roland Kaunas
Keyword(s):  
Endothelial Cells ◽  
Shear Stress ◽  
Endothelial Cell ◽  
Cell Invasion ◽  
Fluid Shear Stress ◽  
Vascular Endothelial Cells ◽  
Umbilical Vein ◽  
Shear Stresses ◽  
Collagen Gels ◽  
Fluid Shear

We have previously developed a cell culture model to study the effects of angiogenic factors, such as sphingosine-1-phosphate (S1P), on the invasion of endothelial cells into the underlying extracellular matrix. In addition to biochemical stimuli, vascular endothelial cells are subjected to fluid shear stress due to blood flow. The present study is aimed at determining the effects of fluid shear stress on endothelial cell invasion into collagen gels. A device was constructed to apply well-defined fluid shear stresses to confluent human umbilical vein endothelial cells (HUVECs) seeded on collagen gels. Fluid shear stress induced significant increases in cell invasion with a maximal induction at ∼5 dyn/cm2. These results provide evidence that fluid shear stress is a significant stimulus for endothelial cell invasion and may play a role in regulating angiogenesis.

MiR-629-5p promotes the invasion of lung adenocarcinoma via increasing both tumor cell invasion and endothelial cell permeability

Oncogene ◽  
2020 ◽  
Vol 39 (17) ◽  
pp. 3473-3488 ◽  
Author(s):  
Yu Li ◽  
Huibiao Zhang ◽  
Lei Fan ◽  
Jiahui Mou ◽  
Yue Yin ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Tumor Cell ◽  
Lung Adenocarcinoma ◽  
Cell Invasion ◽  
Cell Permeability ◽  
Tumor Cell Invasion ◽  
Endothelial Cell Permeability

Increased endothelial cell retraction and tumor cell invasion by soluble factors derived from pancreatic cancer cells

1997 ◽  
Vol 4 (4) ◽  
pp. 361-368 ◽  
Author(s):  
Shoji Nakamori ◽  
Hirotaka Okamoto ◽  
Toshiyuki Kusama ◽  
Kiyoko Shinkai ◽  
Mutsuko Mukai ◽  
...  
Keyword(s):  
Pancreatic Cancer ◽  
Endothelial Cell ◽  
Tumor Cell ◽  
Cancer Cells ◽  
Cell Invasion ◽  
Tumor Cell Invasion ◽  
Soluble Factors ◽  
Pancreatic Cancer Cells ◽  
Endothelial Cell Retraction ◽  
Cell Retraction

Platelet‐activating factor (PAF) induces activation of matrix metalloproteinase 2 activity and vascular endothelial cell invasion and migration

2004 ◽  
Vol 18 (3) ◽  
pp. 568-570 ◽  
Author(s):  
T. William Axelrad ◽  
Dayanand D. Deo ◽  
Paulo Ottino ◽  
Jennefer Van Kirk ◽  
Nicolas G. Bazan ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Matrix Metalloproteinase ◽  
Cell Invasion ◽  
Vascular Endothelial Cell ◽  
Platelet Activating Factor ◽  
Matrix Metalloproteinase 2 ◽  
Vascular Endothelial ◽  
Invasion And Migration ◽  
And Migration

scholarly journals Small Molecule Inhibitors Limit Endothelial Cell Invasion by Staphylococcus aureus

2014 ◽  
Vol 15 (8) ◽  
pp. 727-737 ◽  
Author(s):  
Diana Cordero ◽  
Christopher Fullenkamp ◽  
Rachel Pelly ◽  
Katie Reed ◽  
Lindy Caffo ◽  
...  
Keyword(s):  
Staphylococcus Aureus ◽  
Endothelial Cell ◽  
Small Molecule ◽  
Cell Invasion ◽  
Small Molecule Inhibitors
Sign in / Sign up

Export Citation Format

Share Document