Relationship between Soil Bacterial Communities and Dissolved Organic Matter in a Subtropical Pinus Taiwanensis Forest after Short-Term Nitrogen Addition

ABSTRACT Degradation of agricultural land and the resulting loss of soil biodiversity and productivity are of great concern. Land-use management practices can be used to ameliorate such degradation. The soil bacterial communities at three separate arable farms in eastern England, with different farm management practices, were investigated by using a polyphasic approach combining traditional soil analyses, physiological analysis, and nucleic acid profiling. Organic farming did not necessarily result in elevated organic matter levels; instead, a strong association with increased nitrate availability was apparent. Ordination of the physiological (BIOLOG) data separated the soil bacterial communities into two clusters, determined by soil type. Denaturing gradient gel electrophoresis and terminal restriction fragment length polymorphism analyses of 16S ribosomal DNA identified three bacterial communities largely on the basis of soil type but with discrimination for pea cropping. Five fields from geographically distinct soils, with different cropping regimens, produced highly similar profiles. The active communities (16S rRNA) were further discriminated by farm location and, to some degree, by land-use practices. The results of this investigation indicated that soil type was the key factor determining bacterial community composition in these arable soils. Leguminous crops on particular soil types had a positive effect upon organic matter levels and resulted in small changes in the active bacterial population. The active population was therefore more indicative of short-term management changes.

Download Full-text

Carbon management practices regulate soil bacterial communities in response to nitrogen addition in a pine forest

Plant and Soil ◽

10.1007/s11104-020-04570-9 ◽

2020 ◽

Vol 452 (1-2) ◽

pp. 137-151

Author(s):

Lu Yang ◽

Na Wang ◽

Ye Chen ◽

Wen Yang ◽

Dashuan Tian ◽

...

Keyword(s):

Bacterial Communities ◽

Management Practices ◽

Nitrogen Addition ◽

Pine Forest ◽

Carbon Management ◽

Soil Bacterial Communities ◽

Soil Bacterial

Download Full-text

The Toxicity Exerted by the Antibiotic Sulfadiazine on the Growth of Soil Bacterial Communities May Increase over Time

International Journal of Environmental Research and Public Health ◽

10.3390/ijerph17238773 ◽

2020 ◽

Vol 17 (23) ◽

pp. 8773

Author(s):

Vanesa Santás-Miguel ◽

Laura Rodríguez-González ◽

Avelino Núñez-Delgado ◽

Montserrat Díaz-Raviña ◽

Manuel Arias-Estévez ◽

...

Keyword(s):

Bacterial Growth ◽

Bacterial Communities ◽

Agricultural Soils ◽

Soil Samples ◽

Short Term ◽

Soil Bacterial Communities ◽

Soil Bacterial ◽

First Time ◽

Toxicity Effects

The toxicity exerted by the antibiotic sulfadiazine on the growth of soil bacterial communities was studied in two agricultural soils for a period of 100 days. In the short-term (2 days of incubation), the effect of sulfadiazine on bacterial growth was low (no inhibition or inhibition <32% for a dose of 2000 mg·kg−1). However, sulfadiazine toxicity increased with time, achieving values of 40% inhibition, affecting bacterial growth in both soils after 100 days of incubation. These results, which were here observed for the first time for any antibiotic in soil samples, suggest that long-term experiments would be required for performing an adequate antibiotics risk assessment, as short-term experiments may underestimate toxicity effects.

Download Full-text

Effects of precipitation change and nitrogen addition on the composition, diversity, and molecular ecological network of soil bacterial communities in a desert steppe

PLoS ONE ◽

10.1371/journal.pone.0248194 ◽

2021 ◽

Vol 16 (3) ◽

pp. e0248194

Author(s):

Meiqing Jia ◽

Zhiwei Gao ◽

Huijun Gu ◽

Chenyu Zhao ◽

Meiqi Liu ◽

...

Keyword(s):

Bacterial Communities ◽

Nitrogen Addition ◽

N Deposition ◽

Precipitation Change ◽

Total Carbon ◽

Desert Steppe ◽

Ecological Network ◽

Soil Bacterial Communities ◽

Soil Bacterial ◽

Precipitation Changes

Currently, the impact of changes in precipitation and increased nitrogen(N) deposition on ecosystems has become a global problem. In this study, we conducted a 8-year field experiment to evaluate the effects of interaction between N deposition and precipitation change on soil bacterial communities in a desert steppe using high-throughput sequencing technology. The results revealed that soil bacterial communities were sensitive to precipitation addition but were highly tolerant to precipitation reduction. Reduced precipitation enhanced the competitive interactions of soil bacteria and made the ecological network more stable. Nitrogen addition weakened the effect of water addition in terms of soil bacterial diversity and community stability, and did not have an interactive influence. Moreover, decreased precipitation and increased N deposition did not have a superimposed effect on soil bacterial communities in the desert steppe. Soil pH, moisture content, and NH4+-N and total carbon were significantly related to the structure of bacterial communities in the desert steppe. Based on network analysis and relative abundance, we identified Actinobacteria, Proteobacteria, Acidobacteria and Cyanobacteria members as the most important keystone bacteria that responded to precipitation changes and N deposition in the soil of the desert steppe. In summary, we comprehensively analyzed the responses of the soil bacterial community to precipitation changes and N deposition in a desert steppe, which provides a model for studying the effects of ecological factors on bacterial communities worldwide.

Download Full-text

Soil Bacterial Community Responses to N Application and Warming in a Qinghai-Tibetan Plateau Alpine Steppe

Frontiers in Ecology and Evolution ◽

10.3389/fevo.2021.709518 ◽

2021 ◽

Vol 9 ◽

Author(s):

Zhiyuan Mu ◽

Shikui Dong ◽

Yaoming Li ◽

Shuai Li ◽

Hao Shen ◽

...

Keyword(s):

Tibetan Plateau ◽

Bacterial Community ◽

Bacterial Communities ◽

Nitrogen Application ◽

Soil Bacterial Diversity ◽

Short Term ◽

N Application ◽

Soil Bacterial Communities ◽

Alpine Steppe ◽

Soil Bacterial

Nitrogen deposition and climate warming can alter soil bacterial communities. However, the response of soil bacteria in an alpine steppe to these changes is largely unknown. In this study, a field experiment was performed on the northeastern Qinghai-Tibetan Plateau to determine the changes in soil bacterial communities of alpine steppes in response to nitrogen application and warming. The experiment consisted of four treatments, namely no-N application with no-warming (CK), N application (8 kg N ha−1 year−1) with no-warming (N), warming with no-N application (W), and N application (8 kg N ha−1 year−1) with warming (W&N). This study aimed to investigate (1) the changes in soil bacterial diversity and community structure under simulated nitrogen deposition and warming conditions, and (2) the key environmental factors responsible for these changes. Based on the results, soil bacterial diversity and community composition did not change significantly in the short term. Warming had a significant effect on overall bacterial composition, rare species composition, and individual bacterial taxa. Besides, the interaction between nitrogen application and warming had a significant effect on community β-diversity. Above-ground plant variables were highly correlated with bacterial community characteristics. Nitrogen application and warming did not significantly alter the distribution range of the bacterial community. Overall, this study suggests that soil bacterial communities can remain relatively stable at the level of simulated nitrogen application and warming and that short-term climatic changes may have no significant impacts on soil bacterial communities.

Download Full-text