A Simple Biosensor Based on Positively Charged Graphene Oxide (pGO25000) and Exonuclease I-Assisted Strategy for Hg 2+ Detection

2021 ◽  
Author(s):  
Jieqiong Qiu ◽  
Ting Sun ◽  
Xian Li ◽  
Xiaochuan Jin ◽  
Ziyi Wu ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Exonuclease I ◽  
Positively Charged

scholarly journals Graphene Deposition on Glass Fibers by Triboelectrification

2021 ◽  
Vol 11 (7) ◽  
pp. 3123
Author(s):  
Haroon Mahmood ◽  
Laura Simonini ◽  
Andrea Dorigato ◽  
Alessandro Pegoretti
Keyword(s):  
Graphene Oxide ◽  
Interfacial Shear Strength ◽  
Glass Fibers ◽  
Deposition Process ◽  
Solution Concentration ◽  
Dip Coating ◽  
Slight Improvement ◽  
Reduced Graphene ◽  
Fiber Matrix ◽  
Positively Charged

In this work, a novel nanomaterial deposition technique involving the triboelectrification (TE) of glass fibers (GF) to create attractive charges on their surface was investigated. Through TE, continuous GF were positively charged thus, attracting negatively charged graphene oxide (GO) nanoparticles dispersed in a solution. The electrical charges on the glass fibers surface increased with the intensity of the TE process. The deposited GO coating was then chemically treated to obtain reduced graphene oxide (rGO) on the surface of GFs. The amount of coating obtained increased with the GO solution concentration used during the deposition process, as revealed by FESEM analysis. However, the same increment could not be noticed as a function of the intensity of the process. Both uncoated and coated GF were used to obtain single fiber microcomposites by using a bicomponent epoxy matrix. The fiber/matrix interfacial shear strength was evaluated through micro debonding tests, which revealed an increment of fiber/matrix adhesion up to 45% for rGO coated GF in comparison to the uncoated ones. A slight improvement in the electrical conductivity of rGO coated fibers through TE compared to conventional dip coating was also observed in terms of volumetric resistivity by a four-point probe setup.

One-step and ultrasensitive ATP detection by using positively charged nano-gold@graphene oxide as a versatile nanocomposite

2020 ◽  
Vol 412 (11) ◽  
pp. 2487-2494 ◽  
Author(s):  
Ning Xue ◽  
Shujie Wu ◽  
Zongbing Li ◽  
Xiangmin Miao
Keyword(s):  
Graphene Oxide ◽  
Nano Gold ◽  
One Step ◽  
Positively Charged ◽  
Atp Detection

Fluorometric determination of the activity of uracil-DNA glycosylase by using graphene oxide and exonuclease I assisted signal amplification

2019 ◽  
Vol 186 (2) ◽  
Author(s):  
Mingjian Chen ◽  
Wenkai Li ◽  
Changbei Ma ◽  
Kefeng Wu ◽  
Hailun He ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Signal Amplification ◽  
Dna Glycosylase ◽  
Fluorometric Determination ◽  
Uracil Dna Glycosylase ◽  
Exonuclease I

Graphene nanosheets encapsulated α-MoO3 nanoribbons with ultrahigh lithium ion storage properties

CrystEngComm ◽  
2014 ◽  
Vol 16 (29) ◽  
pp. 6745-6755 ◽  
Author(s):  
Pei-Jie Lu ◽  
Ming Lei ◽  
Jun Liu
Keyword(s):  
Graphene Oxide ◽  
Self Assembly ◽  
Graphene Nanosheets ◽  
Lithium Ion ◽  
Ion Storage ◽  
Storage Properties ◽  
Positively Charged

A facile and effective method has been reported to synthesize graphene-encapsulated α-MoO3 nanoribbons by self-assembly of negatively charged graphene oxide and positively charged MoO3 nanoribbons.

Graphene Oxide and Metal-Mediated Base Pairs Based “Molecular Beacon” Integrating with Exonuclease I for Fluorescence Turn-on Detection of Biothiols

Small ◽  
2014 ◽  
Vol 10 (16) ◽  
pp. 3412-3420 ◽  
Author(s):  
Xiaojing Xing ◽  
Ying Zhou ◽  
Xueguo Liu ◽  
Daiwen Pang ◽  
Hongwu Tang
Keyword(s):  
Graphene Oxide ◽  
Molecular Beacon ◽  
Base Pairs ◽  
Exonuclease I ◽  
Turn On ◽  
Fluorescence Turn On

scholarly journals Quenching of Fluorescence Caused by Graphene Oxide as an Immunosensing Platform in a Microwell Plate Format

Proceedings ◽  
2020 ◽  
Vol 60 (1) ◽  
pp. 60
Author(s):  
Edwin J. Ortiz-Riaño ◽  
Mariana D. Avila-Huerta ◽  
Diana L. Mancera-Zapata ◽  
Eden Morales-Narváez
Keyword(s):  
Graphene Oxide ◽  
Prostate Specific Antigen ◽  
Electrostatic Interactions ◽  
Limit Of Detection ◽  
Specific Antigen ◽  
Human Igg ◽  
Surface Modified ◽  
Microwell Plate ◽  
Oxide Adhesion ◽  
Positively Charged

Immunoassays are, at present, an important tool for diagnostics, drug development, and environmental monitoring. However, most immunoassays involve procedures that require many elements for their development. We introduce a novel biosensing platform based on fluorescence quenching caused by graphene oxide (GO) for the detection of Human-IgG and Prostate-Specific Antigen (PSA). We employ a single antibody for the capture and detection processes, avoiding washing steps. FITC fluorophore was conjugated with antibodies for H-IgG detection, whereas quantum dots were conjugated with antibodies for PSA detection. The simple biosensing platform consists of covering a 96-well microplate (with a polystyrene bottom) with GO. The graphene oxide adhesion is possible by way of electrostatic interactions between the plate surface modified with amino groups (positively charged) and the graphene oxide (negatively charged). This proposal showed an excellent response for the detection of Human-IgG, with acceptable precision (from 0.27% to 5%). The limit of detection reached for H-IgG was 3.35 ng mL-1. In the same manner, for PSA detection, the limit of detection reached was 0.02 ng mL-1 and the precision range was from 0.7% to 15.2%. Furthermore, this biosensing platform was demonstrated to operate with real samples of human urine doped with different concentrations of prostate-specific antigen.

scholarly journals Quaternized polysulfone and graphene oxide nanosheet derived low fouling novel positively charged hybrid ultrafiltration membranes for protein separation

RSC Advances ◽  
2015 ◽  
Vol 5 (63) ◽  
pp. 51208-51219 ◽  
Author(s):  
Mahendra Kumar ◽  
Declan McGlade ◽  
Mathias Ulbricht ◽  
Jenny Lawler
Keyword(s):  
Graphene Oxide ◽  
Charge Density ◽  
Cross Section ◽  
Protein Separation ◽  
Solution Casting ◽  
Ultrafiltration Membranes ◽  
Sem Image ◽  
Graphene Oxide Nanosheet ◽  
Uf Membranes ◽  
Positively Charged

Low fouling positively charged hybrid UF membranes with adjustable charge density fabricated from a blend of PSf/QPSf and GO nanosheets by solution casting and NIPS method. Cross-section SEM image and observed lysozyme transport values at varied pH.

Positively charged graphene oxide nanoparticle: precisely label the plasma membrane of live cell and sensitively monitor extracellular pH in situ

2014 ◽  
Vol 50 (28) ◽  
pp. 3695-3698 ◽  
Author(s):  
Huarui Zhu ◽  
Liang Gao ◽  
Xinglu Jiang ◽  
Ru Liu ◽  
Yueteng Wei ◽  
...  
Keyword(s):  
Graphene Oxide ◽  
Plasma Membrane ◽  
Positive Charge ◽  
Extracellular Ph ◽  
Live Cells ◽  
Oxide Nanoparticles ◽  
Ph Variation ◽  
Oxide Nanoparticle ◽  
Positively Charged

Graphene oxide nanoparticles with positive charge can be developed as fluorescent nanoprobes to detect extracellular pH variation of live cells.

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