DNA Barcode of Tilapia Fish Fillet from the Brazilian Market and a Standardized Coi Haplotyping for Molecular Identification of Oreochromis Spp. (Actinopterygii, Cichlidae)

2022 ◽  
Author(s):  
Beatriz Miguez Nascimento ◽  
Thiago Silva de Paula ◽  
Paulo Marques Machado Brito
Keyword(s):  
Molecular Identification ◽  
Dna Barcode ◽  
Fish Fillet

Research Paper DNA barcode identification of fish products from Guiyang markets in southwest China

2022 ◽  
Author(s):  
Qian Tang ◽  
Qi Luo ◽  
Qian Duan ◽  
Lei Deng ◽  
Renyi Zhang
Keyword(s):  
Dna Barcoding ◽  
Molecular Identification ◽  
Fish Consumption ◽  
Southwest China ◽  
Dna Barcode ◽  
Guizhou Province ◽  
Accurate Identification ◽  
Continuous Growth ◽  
Fish Products ◽  
Fresh Frozen

Nowadays, the global fish consumption continues to rise along with the continuous growth of the population, which has led to the dilemma of overfishing of fishery resources. Especially high-value fish that are overfished are often replaced by other fish. Therefore, the accurate identification of fish products in the market is a problem worthy of attention. In this study, full-DNA barcoding (FDB) and mini-DNA barcoding (MDB) used to detect the fraud of fish products in Guiyang, Guizhou province in China. The molecular identification results showed that 39 of the 191 samples were not consistent with the labels. The mislabelling of fish products for fresh, frozen, cooked and canned were 11.70%, 20.00%, 34.09% and 50.00%, respectively. The average kimura 2 parameter distances of MDB within species and genera were 0.27% and 5.41%, respectively; while average distances of FDB were 0.17% within species and 6.17% within genera. In this study, commercial fraud is noticeable, most of the high-priced fish were replaced of low-priced fish with a similar feature. Our study indicated that DNA barcoding is a valid tool for the identification of fish products and that it allows an idea of conservation and monitoring efforts, while confirming the MDB as a reliable tool for fish products.

Molecular identification of sugarcane black bug (Cavelarius excavates) from Pakistan using cytochrome C oxidase I (COI) gene as DNA barcode

2020 ◽  
Vol 40 (4) ◽  
pp. 1119-1124
Author(s):  
Khadim Hussain ◽  
Kamran Rashid ◽  
Faisal Hafeez ◽  
Ikrma Amad ◽  
Muhammad Ashraf
Keyword(s):  
Cytochrome C ◽  
Cytochrome C Oxidase ◽  
Molecular Identification ◽  
Dna Barcode ◽  
Coi Gene ◽  
Cytochrome C Oxidase I

scholarly journals Molecular Identification and Traceability of Illegal Trading inLignobrycon myersi(Teleostei: Characiformes), a Threatened Brazilian Fish Species, Using DNA Barcode

2016 ◽  
Vol 2016 ◽  
pp. 1-7 ◽  
Author(s):  
Alexandre dos Santos Rodrigues ◽  
José Henrique Souza Galdino Brandão ◽  
Jamille de Araújo Bitencourt ◽  
Ricardo Jucá-Chagas ◽  
Iracilda Sampaio ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Fish Species ◽  
Human Impacts ◽  
Local Population ◽  
Dna Barcode ◽  
Northeastern Brazil ◽  
Genetic Stocks ◽  
Population Structuring ◽  
Fresh Frozen ◽  
Coi Sequences

Lignobrycon myersiis a threatened freshwater fish species and endemic of a few coastal rivers in northeastern Brazil. Even though the Brazilian laws prohibit the fisheries of threatened species,L. myersiis occasionally found in street markets, being highly appreciated by local population. In order to provide a reliable DNA barcode dataset forL. myersi, we compared mitochondrial sequences of cytochrome c oxidase subunit I (COI) from fresh, frozen, and salt-preserved specimens. Phylogenetically related species (Triportheusspp.) and other fish species (Astyanax fasciatus) commonly mixed withL. myersiin street markets were also included to test the efficiency of molecular identification. In spite of the differences in conservation processes and advanced deterioration of some commercial samples, high-quality COI sequences were obtained and effective in discriminatingL. myersispecimens. In addition, while populations from Contas and Almada River basins seem to comprise a single evolutionary lineage, the specimens from Cachoeira River were genetically differentiated, indicating population structuring. Therefore, DNA barcoding has proved to be useful to trace the illegal trading ofL. myersiand to manage threatened populations, which should focus on conservation of distinct genetic stocks and mitigation on human impacts along their range.

scholarly journals Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene

2020 ◽  
Vol 21 (7) ◽  
Author(s):  
Van Basten Tambunan ◽  
Ardha Apriyanto ◽  
Walter Ajambang ◽  
Culbertson Enow Etta ◽  
Bandung Sahari ◽  
...  
Keyword(s):  
Genetic Variation ◽  
Molecular Identification ◽  
Oil Palm ◽  
Genetic Study ◽  
Population Genetic ◽  
Mitochondrial Gene ◽  
Dna Barcode ◽  
Coi Gene ◽  
Population Genetic Study ◽  
Elaeidobius Kamerunicus

Abstract. Tambunan VB, Apriyanto A, Ajambang W, Etta CE, Sahari B, Buchori D, Hidayat P. 2020. Molecular identification and population genetic study of Elaeidobius kamerunicus Faust. (Coleoptera: Curculionidae) from Indonesia, Malaysia and Cameroon based on mitochondrial gene. Biodiversitas 21: 3263-3270. Oil palm pollinating weevil Elaeidobius kamerunicus is a very important insect pollinator in oil palm plantation. However, there is still lack of information about molecular identification and population genetic study in this species. The purpose of this study was to explore the effectiveness of oil palm pollinating weevil identification using mitochondrial DNA of COI gene and to assess its genetic variation between different locations and countries. We sequenced the DNA barcode of 36 individuals of this species using the mtDNA Cytochrome Oxidase I (COI) gene to explore their genetic variation, identity and phylogenetic relationship. The COI gene sequences generated from this study were successful in identifying E. kamerunicus. Phylogenetic analysis also revealed 3 well-supported monophyletic haplogroups of E. kamerunicus population. In addition, genetic differentiation analysis revealed that most populations from Indonesia were different from Malaysian and Cameroonian populations indicating that there was a genetic variation between the population samples from these countries. The overall E. kamerunicus used in this study were geographically structured in two regions; outside Indonesia region (Cameroon and Malaysia) and Indonesia region. These results demonstrate the feasibility of using COI gene sequence for molecular identification and population genetic study of E. kamerunicus species.

Molecular identification and DNA barcode screening of Acaroid mites in ground flour dust

Genome ◽  
2021 ◽  
Author(s):  
Wanyu Zhang ◽  
Juan Cheng ◽  
Yae Zhao ◽  
Dongling Niu ◽  
Hongsong Guo
Keyword(s):  
Molecular Identification ◽  
Dna Barcode ◽  
Molecular Data ◽  
Universal Primers ◽  
Dna Barcodes ◽  
Dermatophagoides Farinae ◽  
Tyrophagus Putrescentiae ◽  
Multiple Target ◽  
Morphological Classification ◽  
Flour Dust

Molecular identification of acaroid mites is difficult because of the scarcity of molecular data in GenBank. Here, acaroid mites collected from ground flour dust in Xi'an China were preliminarily morphologically classified/grouped. Universal primers were then designed to amplify and screen suitable DNA barcodes for identifying these mites. Sixty mite samples were morphologically classified into six groups. Groups 1–2 were identified to Dermatophagoides farinae, and Tyrophagus putrescentiae; while Groups 3–6 were not identified to the species level. ITS2 exhibited higher efficiency in molecular identification in comparison with COI, 12S, and 16S. Groups 1–6 were identified as D. farinae, T. putrescentiae, Suidasia nesbitti, Chortoglyphus arcuatus, Lepidoglyphus destructor, and Gohieria sp., respectively. The phylogenetic results were consistent with the morphological classification. Group 6 was further identified as G. fusca according to the morphology of reproductive foramen. We conclude that the use of ITS2 and the availability of universal primers provides an ideal DNA barcode for molecular identification of acaroid mites. The use of multiple target genetic markers in conjunction with morphological approaches will improve the accuracy of Acaridida identification. Key words: acaroid mites, Taxonomy, universal primers, molecular identification, DNA barcode.

DNA barcode-based molecular identification system for fish species

2010 ◽  
Vol 30 (6) ◽  
pp. 507-512 ◽  
Author(s):  
Sungmin Kim ◽  
Hae-Seok Eo ◽  
Hyeyoung Koo ◽  
Jun-Kil Choi ◽  
Won Kim
Keyword(s):  
Molecular Identification ◽  
Fish Species ◽  
Dna Barcode ◽  
Identification System

scholarly journals Molecular Identification of Dendrobium Species (Orchidaceae) Based on the DNA Barcode ITS2 Region and Its Application for Phylogenetic Study

2015 ◽  
Vol 16 (9) ◽  
pp. 21975-21988 ◽  
Author(s):  
Shangguo Feng ◽  
Yan Jiang ◽  
Shang Wang ◽  
Mengying Jiang ◽  
Zhe Chen ◽  
...  
Keyword(s):  
Molecular Identification ◽  
Dna Barcode ◽  
Phylogenetic Study ◽  
Its2 Region

scholarly journals DNA Barcode for the Parthenogenetic Surinam Cockroach Pycnoscelus surinamensis (Linnaeus, 1758) (Blattodea: Blaberidae) from India

2022 ◽  
Vol 7 (1) ◽  
pp. 1-7
Author(s):  
A Shabnam ◽  
K P Dinesh
Keyword(s):  
Dna Barcoding ◽  
Molecular Identification ◽  
Western Ghats ◽  
Dna Barcode ◽  
Insect Fauna ◽  
Mt Dna ◽  
Faunal Diversity ◽  
Southern Western Ghats

DNA Barcoding is one of the emerging tools in molecular identification of faunal diversity, specifically insect fauna. The Surinam cockroach, Pycnoscelus surinamensis is the only known roach to be obligatorily parthenogenetic, with reported haplotypes. P. surinamensis is well established in Indomalayan, tropical and subtropical regions and substantially documented from India with a phenetic approach. Herewith we report the first set of mt DNA barcode from a vouchered collection for the species from southern Western Ghats India. Discussions are made on the identity of two sequences each of Blatteria species and Pycnoscelus species reported from USA.

scholarly journals Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis

2018 ◽  
Vol 19 (2) ◽  
pp. 364-368
Author(s):  
LULUT DWI SULISTYANINGSIH ◽  
MARLINA ARDIYANI ◽  
ABINAWANTO ABINAWANTO ◽  
ANDI SALAMAH
Keyword(s):  
Phylogenetic Analysis ◽  
Dna Barcoding ◽  
Molecular Identification ◽  
Short Communication ◽  
Pharmacological Study ◽  
Dna Barcode ◽  
Phylogenetic Reconstruction ◽  
Steroidal Saponin ◽  
Morphological Variations ◽  
Molecular Approaches

Sulistyaningsih LD, Abinawanto, Ardiyani M, Salamah A. 2018. Short Communication: Phylogenetic analysis and molecular identification of Canar (Smilax spp.) in Java, Indonesia Based on DNA Barcoding Analysis. Biodiversitas 19: 364-368. Smilax spp. (Smilacaceae) has long been used as medicinal herbs especially in East Asia and North America as they were known to be rich in steroidal saponin. Pharmacological study has been carried out in Indonesia. This genus is widespread in Indonesia and fairly abundant in Java and has been known either as edible fruit or medicinal plants. Characteristics of Smilax as a dioecious plant with high morphological variations make it thorny in species identification. Various molecular approaches have been devised to overcome identification problems such as DNA barcoding. This study, therefore was conducted to analyze the DNA barcoding application for phylogenetic and identification of Smilax in Java. A total of 31 samples were used in this study including 19 accession numbers from NCBI GeneBank. The genus Ripogonum was used as the out-group in phylogenetic reconstruction. Samples were successfully extracted by CTAB method with some modifications. rbcL region was used as the DNA barcode showed sufficient variation and conserved flanks. Two unidentified specimens have high similarity with S. leucophyla and lies in the same clade. The phylogenetic tree constructed by Maximum Likelihood analysis. The result showed that the monophyletic of Smilacaceae consisted of four clades. The genus Heterosmilax nested with Smilax though with low bootstraps value. It supports the monogeneric status of Smilacaceae.

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