scholarly journals Evaluating of HPV–DNA ISH as an adjunct to p16 testing in oropharyngeal cancer

2020 ◽  
pp. FSO606
Author(s):  
Jeffrey Chi ◽  
Isabel R Preeshagul ◽  
Silvat Sheikh-Fayyaz ◽  
Sewit Teckie ◽  
Nina Kohn ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Wide Spectrum ◽  
Current Data ◽  
Oropharyngeal Carcinoma ◽  
Hpv Dna ◽  
P16 Immunohistochemistry ◽  
Agreement Coefficient ◽  
Current Guidelines

Aim: Current guidelines recommend p16 immunohistochemistry (IHC) for testing human papillomavirus (HPV) in oropharyngeal carcinoma (OPSCC). We evaluated the value of adding DNA in situ hybridization (ISH) to p16 IHC. Methods: Fifty patients with OPSCC were analyzed. Concordance between HPV–DNA ISH and p16 IHC was measured by Gwet's agreement coefficient. Results: p16 IHC was positive in 35/48 (72.9%), negative in 8/48 (16.7%) patients. Wide spectrum DNA–ISH was positive in 9/23 (39%) and negative in 14/23 (60.9%) patients. High-risk 16/18 (HR) HPV DNA–ISH was positive in 11/23 (47.8%) and negative in 12 (52.2%) patients. The agreement between HPV DNA–ISH and p16 IHC is fair (Gwet's AC1 = 0.318). Conclusion: The agreement between p16 IHC and HPV–DNA ISH was fair. However, ISH sensitivity was low. Our findings add to the current data that p16 IHC testing is reliable and may be enough as a stand-alone test for HPV detection in OPSCC.

Human Papillomavirus (HPV) Cervical Lesions: Results from 300 Italian Women Studied with Dna Hybridization Techniques and Morphology

1988 ◽  
Vol 74 (6) ◽  
pp. 745-749 ◽  
Author(s):  
Fiorella Nuzzo ◽  
Vittorio Tison ◽  
Antonella Castagnoli ◽  
Maurizio Tiboni ◽  
Ethel-Michele De Villiers ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Southern Blot ◽  
Dna Hybridization ◽  
Blot Hybridization ◽  
Hpv Infection ◽  
Cervical Lesions ◽  
Hpv Dna ◽  
Cervical Infection

Human papillomavirus cervical infection was investigated in a series of 300 unselected women by comparing morphological diagnoses (cytology and histology) with results of DNA hybridization techniques (filter in situ hybridization of DNA from exfoliated cervical cells and Southern blot analysis of HPV-DNA in cervical biopsy specimens). The prevalence of HPV cervical infection diagnosed by PAP smears was 11.6 %. Despite disadvantages, filter in situ hybridization was confirmed to be particularly useful for screening purposes to detect HPV in cervical scrapings. In 3 cases it was the only applicable method for diagnosing « high-risk » HPV infection. Southern blot hybridization of tissue DNA with HPV 16-DNA revealed the presence of this virus in 8 cases, and HPV 31-DNA and HPV 42-DNA in 1 case each.

Sensitivity of in situ hybridization techniques using biotin- and 35S-labeled human papillomavirus (HPV) DNA probes

1988 ◽  
Vol 19 (3-4) ◽  
pp. 225-238 ◽  
Author(s):  
S. Syrjänen ◽  
P. Partanen ◽  
R. Mäntyjärvi ◽  
K. Syrjänen
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Dna Probes ◽  
Hpv Dna

scholarly journals Sensitive Chemiluminescence In Situ Hybridization for the Detection of Human Papillomavirus Genomes in Biopsy Specimens

1997 ◽  
Vol 45 (5) ◽  
pp. 729-735 ◽  
Author(s):  
Monica Musiani ◽  
Marialuisa Zerbini ◽  
Simona Venturoli ◽  
Giovanna Gentilomi ◽  
Giorgio Gallinella ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Human Papillomavirus ◽  
Colorimetric Detection ◽  
Optical Microscope ◽  
Great Sensitivity ◽  
Transmitted Light ◽  
Hpv Dna ◽  
Biopsy Specimens ◽  
Luminescent Signal

We developed a sensitive chemiluminescence in situ hybridization assay for detection of human papillomavirus (HPV) DNA for objective and semiquantitative evaluation of the results. The hybridization reaction was performed using either digoxigenin-, biotin-, or fluorescein-labeled probes, visualized with alkaline phosphatase as the revealing enzyme and a highly sensitive 1,2 dioxetane phosphate as chemiluminescent substrate. The light emitted from the hybridized probes was detected, analyzed, and measured using a high-performance, low light-level imaging luminograph connected to an optical microscope and to a personal computer for quantification of the photon fluxes and for image analysis. The system operated in consecutive steps: First, hybridized specimens were recorded in transmitted light. Then the net luminescent signal was recorded, and then an overlay of the two images provided by the transmitted light and by the luminescent signal allowed the spatial distribution of the target DNA to be localized, measured, and evaluated. Biopsy specimens from different pathological conditions associated with HPV, which had previously been proved positive for HPV DNA with the polymerase chain reaction (PCR), were analysed. The chemiluminescence in situ hybridization proved sensitive and specific with digoxigenin-, biotin-, or fluorescein-labeled probes, and provided an objective evaluation of the results. The results obtained with chemiluminescence in situ hybridization were also compared with results obtained with in situ hybridization with colorimetric detection, with good concordance of the data. Chemiluminescence in situ hybridization therefore offers the possibility of detecting HPV DNA with great sensitivity in biopsy specimens. Moreover, the images of the samples, stored in the computer, are a permanent record of the reaction and can also be sent for evaluation or comparison to other laboratories using computer networks.

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