scholarly journals Comparison of SOX-10, HMB-45, and Melan-A in Benign Melanocytic Lesions

2021 ◽  
Vol Volume 14 ◽  
pp. 1419-1425
Author(s):  
Sabrina E Dass ◽  
Taryn Huizenga ◽  
Mehdi Farshchian ◽  
Darius R Mehregan
Keyword(s):  
Melanocytic Lesions ◽  
Hmb 45

Differential Expression of Melanocytic Markers in Myoid, Lipomatous, and Vascular Components of Renal Angiomyolipomas

2007 ◽  
Vol 131 (1) ◽  
pp. 122-125 ◽  
Author(s):  
Andres A. Roma ◽  
Cristina Magi-Galluzzi ◽  
Ming Zhou
Keyword(s):  
Smooth Muscle ◽  
Blood Vessel ◽  
Blood Vessels ◽  
Differential Expression ◽  
Tumor Cells ◽  
Tissue Microarray ◽  
Expression Patterns ◽  
Renal Angiomyolipoma ◽  
Melanocytic Lesions ◽  
Hmb 45

Abstract Context.—Renal angiomyolipoma is a tumor composed of varying amounts of fat, smooth muscle, and blood vessels. Characteristically, tumor cells express melanocytic markers such as HMB-45 and Melan-A. Recently, several other markers have been described as having excellent diagnostic sensitivity in cutaneous melanocytic lesions. Objectives.—To compare the sensitivities of 5 melanocytic markers in renal angiomyolipoma and to study the expression patterns of these markers in the 3 different components of angiomyolipoma. Design.—A tissue microarray of 20 renal angiomyolipomas was constructed. For each case, 3 cores containing fat, blood vessels, and smooth muscle were taken. The tissue microarray was then stained for HMB-45, Melan-A, tyrosinase, NK1-C3, and CD117. Results.—HMB-45 was positive in 95%, Melan-A in 85%, NK1-C3 in 70%, tyrosinase in 50%, and CD117 in 40% of the cases. All (20/20) were positive for HMB-45 and Melan-A combined. These 5 markers had different sensitivities in the 3 components. HMB-45 was positive in 90%, 85%, and 80% of fat, smooth muscle, and blood vessel components, respectively; Melan-A in 70%, 60%, and 40%; NK1-C3 in 55%, 55%, and 45%; tyrosinase in 30%, 40%, and 10%; and CD117 in 20%, 40%, and 10%, respectively, of these 3 components. Conclusions.—HMB-45 and Melan-A combined were positive in 100% of the renal angiomyolipomas. We recommend the use of these 2 markers in the workup of this entity, including those with predominantly 1 component. Other melanocytic markers are of limited use. A tissue block comprising predominantly fat or smooth muscle components should be used when performing melanocytic marker immunostain.

scholarly journals Melanocytic lesions: A pilot study using dual HMB-45/Ki67 staining with a new depth measurement to aid diagnosis

2021 ◽  
Vol 8 (9) ◽  
Author(s):  
Darrell J Johnston ◽  
Phuoc-Tan Diep
Keyword(s):  
Pilot Study ◽  
Diagnostic Testing ◽  
Distance Ratio ◽  
Depth Measurement ◽  
Pigmented Lesions ◽  
Melanocytic Lesions ◽  
Significant Difference ◽  
Malignant Group ◽  
The Mean ◽  
Hmb 45

Diagnostic pitfalls have been widely documented on atypical naevi, a group of pigmented lesions that occupy a grey portion between benign and malignant variants. As malignant melanoma is associated with a high mortality rate it is vital we have a reliable diagnostic testing method for these challenging lesions. A pilot study was proposed using dual immunohistochemistry staining with a new depth measurement, the Lancaster depth, to develop a validating tool to measure difficult atypical lesions against. The Lancaster depth was recorded for 68 pigmented lesions; 34 benign and 34 malignant, where measurement was taken from the granular layer to the deepest HMB-45/Ki67 dual stained melanocyte. This measurement was compared against the established Breslow depth measurement where the distance between the two was of interest. The mean malignant Lancaster depth at 1.51mm was higher than that of the benign at 0.12mm. There was a statistically significant difference of 1.40mm, P = <.001. There was a statistically significant difference between the mean Lancaster and Breslow depths in both malignant and benign lesions. In the malignant group this was 0.28mm, P = <.001 compared to the benign which was 1.07mm, P = <.001. A narrow distance ratio between the two depths indicated a malignant diagnosis. A wider distance ratio was indicative of a benign diagnosis. As a diagnostic validator the Lancaster depth shows promises but has limitations as a standalone model. Working in conjunction with the Breslow depth we potentially have a useful adjunct to accurately diagnose difficult atypical lesions.

Ex vivo Quality-Switched Ruby Laser Irradiation of Cutaneous Melanocytic Lesions: Persistence of S-100-, HMB-45- and Masson-Positive Cells

Dermatology ◽  
1997 ◽  
Vol 194 (4) ◽  
pp. 344-350 ◽  
Author(s):  
D. Kopera ◽  
U. Hohenleutner ◽  
W. Stolz ◽  
M. Landthaler
Keyword(s):  
Laser Irradiation ◽  
Ruby Laser ◽  
Ex Vivo ◽  
Melanocytic Lesions ◽  
S 100 ◽  
Hmb 45

HMB-45 Staining in Benign and Malignant Melanocytic Lesions

1991 ◽  
Vol 13 (6) ◽  
pp. 543-550 ◽  
Author(s):  
Henry G. Skelton ◽  
Kathleen J. Smith ◽  
Terry L. Barrett ◽  
George P. Lupton ◽  
James H. Graham
Keyword(s):  
Melanocytic Lesions ◽  
Hmb 45

HMB-45 Immunohistochemical Staining of Conjunctival Melanocytic Lesions

Ophthalmology ◽  
1991 ◽  
Vol 98 (4) ◽  
pp. 453-458 ◽  
Author(s):  
Jan M. McDonnell ◽  
Yan Yu Sun ◽  
David Wagner
Keyword(s):  
Immunohistochemical Staining ◽  
Melanocytic Lesions ◽  
Hmb 45

Immunogold localization of HMB-45 antigen in pulmonary lymphangiomyomatosis

1995 ◽  
Vol 53 ◽  
pp. 998-999
Author(s):  
J.M. Minda ◽  
E. Dessy ◽  
G. G. Pietra
Keyword(s):  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Room Temperature ◽  
Osmium Tetroxide ◽  
Muscle Cells ◽  
Ultrastructural Localization ◽  
Reproductive Age ◽  
Thin Sections ◽  
Smooth Muscle Proliferation ◽  
Hmb 45

Pulmonary lymphangiomyomatosis (PLAM) is a rare disease occurring exclusively in women of reproductive age. It involves the lungs, lymph nodes and lymphatic ducts. In the lungs, it is characterized by the proliferation of smooth muscle cells around lymphatics in the bronchovascular bundles, lobular septa and pleura The nature of smooth muscle proliferation in PLAM is still unclear. Recently, reactivity of the smooth muscle cells for HMB-45, a melanoma-related antigen has been reported by immunohistochemistry. The purpose of this study was the ultrastructural localization of HMB-45 immunoreactivity in these cells using gold-labeled antibodies.Lung tissue from three cases of PLAM, referred to our Institution for lung transplantation, was embedded in either Poly/Bed 812 post-fixed in 1% osmium tetroxide, or in LR White, without osmication. For the immunogold technique, thin sections were placed on Nickel grids and incubated with affinity purified, monoclonal anti-melanoma antibody HMB-45 (1:1) (Enzo Diag. Co) overnight at 4°C. After extensive washing with PBS, grids were treated with Goat-anti-mouse-IgG-Gold (5nm) (1:10) (Amersham Life Sci) for 1 hour, at room temperature.

Conjunctival Melanocytic Lesions

2019 ◽  
pp. 92-93
Author(s):  
E.B. Turkoglu ◽  
Keyword(s):  
Melanocytic Lesions
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