Single-nucleotide polymorphisms of microRNA processing machinery genes are associated with risk for gastric cancer

Purpose Single nucleotide polymorphisms (SNPs) are an important cause of functional variation in proteins leading to tumorigenesis. We aimed to identify candidate biomarkers with polymorphisms in gastric cancer (GC). Methods The SNP microarray profile GSE29996 including 50 GC samples and 50 normal controls, and gene expression data GSE56807 consisting of 5 GC samples and 5 controls were downloaded from the Gene Expression Omnibus database. After preprocessing of raw data, GC-associated SNPs were identified using the Cochran-Armitage trend test, and differentially expressed genes (DEGs) were screened out using the limma package in R. Significant DEGs with risk associated SNP loci were screened using the Fisher combination test. Gene ontology function and pathway enrichment analyses were performed for DEGs with risk associated SNP loci by GenCLip online tool. Transcriptional regulatory analysis was also conducted for transcription factor and target DEGs. Results A total of 79 DEGs with risk associated SNP loci were identified from GC samples compared with normal controls. These DEGs were mainly enriched in anatomical structure development, including embryo development. Additionally, DEGs were significantly involved in the NO1 pathway, including actin, alpha 1, skeletal muscle (ACTA1). In the regulatory network, transcription factor forkhead box L1 (FOXL1) regulated 26 DEGs with risk associated SNP loci, including Iroquois homeobox 1 (IRX1) rs11134044, sex determining region Y (SRY)-box1 (SOX1) rs9549447 and msh homeobox 1 (MSX1) rs41451149. Conclusions IRX1, SOX1 and MSX1 with risk associated SNP loci may serve as candidate biomarkers for diagnosis and prognosis of GC.

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CyclinD1 Single Nucleotide Polymorphisms and Risk of Gastric Cancer among Iranian Patients. A Pilot Genetic Association Study

The American Journal of Gastroenterology ◽

10.14309/00000434-200509001-00125 ◽

2005 ◽

Vol 100 ◽

pp. S64-S65

Author(s):

Minoosh Vossoughi ◽

Ala Melati-Rad ◽

Elham Sharif Panah ◽

Mohammad Reza Zali ◽

Babak Noorinayer

Keyword(s):

Gastric Cancer ◽

Single Nucleotide Polymorphisms ◽

Association Study ◽

Genetic Association ◽

Genetic Association Study ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Iranian Patients

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Black Carbon Exposures, Blood Pressure, and Interactions with Single Nucleotide Polymorphisms in MicroRNA Processing Genes

Environmental Health Perspectives ◽

10.1289/ehp.0901440 ◽

2010 ◽

Vol 118 (7) ◽

pp. 943-948 ◽

Cited By ~ 58

Author(s):

Elissa H. Wilker ◽

Andrea Baccarelli ◽

Helen Suh ◽

Pantel Vokonas ◽

Robert O. Wright ◽

...

Keyword(s):

Blood Pressure ◽

Single Nucleotide Polymorphisms ◽

Black Carbon ◽

Nucleotide Polymorphisms ◽

Single Nucleotide ◽

Microrna Processing

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Polymorphism of MUC1 gene in Vietnamese gastric cancer patients: a case-control study

10.21203/rs.2.14183/v1 ◽

2019 ◽

Author(s):

Lan Thi Ngoc Nguyen ◽

Dzung Thi Ngoc Dang ◽

Van Thanh Ta ◽

Huy Quang Dang ◽

Chuc Van Tran ◽

...

Keyword(s):

Gastric Cancer ◽

Family History ◽

Single Nucleotide Polymorphisms ◽

Cancer Patients ◽

Nucleotide Polymorphisms ◽

Mucin 1 ◽

Single Nucleotide ◽

History Of ◽

Gastric Cancer Patients ◽

Multifactor Regression

Abstract Background Gastric cancer is a malignant type of cancer associated with many factors such as environment, behavior, infection, and genetics, which include Single Nucleotide Polymorphism. A few studies revealed polymorphisms of the Mucin 1 gene have a role and significance as a susceptible factor contributing to gastric cancer. The aim of this research is to evaluate the association between Single Nucleotide Polymorphisms of the Mucin 1 gene and Vietnamese gastric cancer patients.Methods 302 gastric cancer patients and 304 controls were interviewed for social-economic characteristics, smoking and drinking status, personal and family history of gastric diseases. Genotyping was done using polymerase chain reaction-restriction fragment length polymorphism analysis. The association of Single Nucleotide Polymorphisms with gastric cancer was evaluated using multifactor regression models.Results AA genotype for rs4072037 was found to be highly associated with gastric cancer (OR: 2.07 (95% CI: 1.46-2.90). GG genotype for rs2070803 increased the risk of gastric cancer (OR:1.96 (95% CI: 1.37-2.78). These genotypes in combination with other factors such as old age, male gender, alcoholism and personal history of gastric disease also showed an increased risk of having gastric cancer.Conclusions rs4072037 and rs2070803 of Mucin 1 genes are two genotypic risk factors of gastric cancer. Those in combination with other factors such as gender, family history, smoking and drinking habits significantly increase the risk of gastric cancer.

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Single nucleotide polymorphisms associated with gastric cancer in Iranian patients

Immunopathologia Persa ◽

10.34172/ipp.2021.37 ◽

2021 ◽

Vol 7 (2) ◽

pp. e37-e37

Author(s):

Arash Alghasi ◽

Gholamreza Farnoosh ◽

Ali Saeedi Boroujeni ◽

Mohammad Bahadoram ◽

Sima Tahmaseby Gandomkari ◽

...

Keyword(s):

Gastric Cancer ◽

Single Nucleotide Polymorphisms ◽

Large Scale ◽

Nucleotide Polymorphisms ◽

Caspase 9 ◽

Northern Iran ◽

Single Nucleotide ◽

C677t Mthfr ◽

Increased Risk ◽

Iranian Patients

Gastric cancer is one of the leading worldwide cancers formed in the lining of the stomach, and is the most prevalent cancer in northern Iran. Recent interventions for the early diagnosis of gastric cancer are based on genetic susceptibility parameters and the interactions between genes and the environment. Accordingly, this narrative review was designed to summarize the genetic markers involved in Iranian patients with gastric cancer, classified by cellular function. There was a significant relationship between single nucleotide polymorphisms (SNPs) in rs1051208 C allele (RAF1), rs531564 (pri-miR-124-1), rs1053023 (STAT3), rs8193 C allele (CD44), rs3130932G allele (OCT4), rs283821943, rs2032586 (ABCB1), codons 72,248 (p53), -137 G/C (IL-18), Pro12Ala (PPARγ), rs1053023 (STAT3), rs4647603 (caspase 3), -712C>T (caspase 9), -1263 A> (caspase 9) and gastric cancer. Increased risk was observed in C677T (MTHFR). Finally, decreased risk of gastric cancer was explored in -938 C>A (bcl2). Asp299Gly (TLR-4), rs1028181-513T/C (IL-19) Pro12Ala (PPARγ) may play a crucial role in susceptibility of Helicobacter pylori and gastric pathogenesis. According to the findings, the genetic polymorphisms in the immune-associated genes were related to the gastric cancer among the Iranian patients. Therefore, further large-scale functional investigations are needed to draw definite conclusions.

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