scholarly journals ANTIOXIDANT AND CYTOTOXIC ACTIVITY OF CommiphoraMukul(GUGGUL) EXTRACTS AGAINST HeLa CELL-LINE.

2016 ◽  
Vol 4 (5) ◽  
pp. 1258-1267 ◽  
Author(s):  
Shivangi Mathur ◽  
◽  
Rinkal Mulani ◽  
L.B. George ◽  
Chiranjivi Barot ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Hela Cell Line

scholarly journals Novel C-ring Analogs of Ursolic acid: Synthesis and Cytotoxic Evaluation

2014 ◽  
Vol 9 (12) ◽  
pp. 1934578X1400901
Author(s):  
Uppuluri V. Mallavadhani ◽  
Banita Pattnaik ◽  
Nitasha Suri ◽  
Ajit K. Saxena
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Cell Lines ◽  
Ursolic Acid ◽  
Parent Compound ◽  
Acid Synthesis ◽  
Hela Cell Line ◽  
Hela Cell Lines ◽  
Protective Groups

Ursolic acid (1), a natural pentacyclic triterpenic acid, afforded a variety of ring-C transformed products (5–11) when treated with N-bromosuccinimide under the influence of a range of protective groups and solvents. The synthesized compounds have been evaluated for cytotoxic activity against prostate PC 3, leukemia THP 1, cervical Hela and lung A-549 cancer cell lines. Among the tested analogs, compounds 5, 8, 9 and 10 showed potent activity against PC 3, THP 1 and Hela cell lines. Especially, compound 10 showed enhanced activity against the Hela cell line than the parent compound. Compounds 5, 8 and 9 showed comparable activities against PC 3 and THP 1 cell lines.

scholarly journals Methylation of 2-Aryl-2-(3-indolyl)acetohydroxamic Acids and Evaluation of Cytotoxic Activity of the Products

Molbank ◽  
10.3390/m1307 ◽  
2021 ◽  
Vol 2022 (1) ◽  
pp. M1307
Author(s):  
Dmitrii A. Aksenov ◽  
Alexander V. Aksenov ◽  
Lidiya A. Prityko ◽  
Nicolai A. Aksenov ◽  
Liliya V. Frolova ◽  
...  
Keyword(s):  
Antitumor Activity ◽  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Hydroxamic Acid ◽  
Methyl Esters ◽  
Hydroxamic Acids ◽  
Hela Cell Line ◽  
Lead Compounds

2-Aryl-2-(3-indolyl)acetohydroxamic acids demonstrate promising antitumor activity, but quickly metabolize in vivo via glucuronidation of hydroxamic acid residue. In an attempt to improve their pharmacokinetics, methyl esters were synthesized via a newly developed protocol for chemoselective mono-methylation of hydroxamic acids. The cytotoxicity of these derivatives against the HeLa cell line was evaluated and found to be inferior compared to the parent lead compounds.

scholarly journals New Secodaphnane-Type Alkaloids with Cytotoxic Activities from Daphniphyllum angustifolium Hutch

2021 ◽  
Author(s):  
Qing-Yun Lu ◽  
Jia-Hui Zhang ◽  
Ying-Yao Li ◽  
Xue-Xue Pu ◽  
Cui-Shan Zhang ◽  
...  
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Natural Product ◽  
Cell Line ◽  
Cytotoxic Activities ◽  
Spectroscopic Methods ◽  
Hela Cell Line

AbstractOne new Daphniphyllum alkaloid, daphnioldhanol A (1), together with three known ones, were isolated from the stem part of Daphniphyllum angustifolium Hutch. Their structures were elucidated by spectroscopic methods and comparing with the literature data. Compound 2 is a new natural product, but known by synthesis as a racemate. Compound 1 exhibited week cytotoxic activity against Hela cell line with IC50 of 31.9 μM. Graphic Abstract

scholarly journals Cytotoxicity of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) on HeLa cell line

2020 ◽  
Vol 1 (2) ◽  
Author(s):  
Adisty Ridha Damasuri ◽  
Eti Nurwening Sholikhah ◽  
Mustofa
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Colorimetric Assay ◽  
Cancer Cell Line ◽  
Positive Control ◽  
Probit Analysis ◽  
Hela Cell Line ◽  
Chalcone Derivatives ◽  
Cell Line Hela

In our previous study, some amino chalcone derivatives have been synthesized and evaluated their cytotoxicity against breast cancer cell line T47D. Among 11 amino chalcone derivatives, ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) exhibited the most active compound.This study aimed to investigate cytotoxic activity of the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) against cervical cell line (HeLa). The cytotoxic activitywas determined using the MTT colorimetric assay. Cisplatin was used as positive control. From this MTT method, inhibitory concentration 50% (IC50) values were determined by probit analysis based on the relationship between log concentrations versus the percentage of cells growth inhibition.The results showed that the IC50 of ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) and cisplatinwere 22.75 ± 19.13 μg/mL and 14.96±1.08 μg/mL, respectively. In conclusion, the ((E)-1-(4-aminophenyl)-3-phenylprop-2-en-1-one)) has moderate cytotoxic activity against HeLa cell line based on National Cancer Institute (NCI) criteria.

scholarly journals In vitro Cytotoxic Activity of Ethyl Acetate Fraction of Hibiscus vitifolius Flowers Against HeLa Cell Line

2018 ◽  
Vol 2 (3) ◽  
pp. 122-125 ◽  
Author(s):  
Gujjula Nishitha ◽  
◽  
Gurram Asha Latha ◽  
Lagadapati Tejaswini ◽  
Amrutha Mounica ◽  
...  
Keyword(s):  
Hela Cell ◽  
Ethyl Acetate ◽  
Cytotoxic Activity ◽  
Cell Line ◽  
Ethyl Acetate Fraction ◽  
Hela Cell Line

scholarly journals ANTICANCER ACTIVITY OF CALANONE ON HeLa CELL LINE

2010 ◽  
Vol 10 (2) ◽  
pp. 240-244 ◽  
Author(s):  
Heny Ekowati ◽  
Indwiani Astuti ◽  
Mustofa Mustofa
Keyword(s):  
Hela Cell ◽  
Cytotoxic Activity ◽  
Anticancer Activity ◽  
Cell Line ◽  
Cervical Carcinoma ◽  
Carcinoma Cell ◽  
Suppressor Gene ◽  
Positive Control ◽  
Hela Cell Line ◽  
P53 Expression

Calanone (coumarin derivate compound), isolated from Calophyllum sp. had been shown to have cytotoxic activity on leukemia L1210 cell line with IC50 = 59.40 mg/mL. Calanone presumed have anticancer activity on HeLa cervical carcinoma cell. This study was conducted to investigate the cytotoxic and apoptotic activity of calanone and its effect to p53 and p21 expression on HeLa cervical carcinoma cell. Cytotoxic assay of calanone was performed on HeLa cell line, using MTT assay. Apoptotic assay was performed on HeLa cell line incubated with calanone for 24 h, by immunofluororescence method, using fluorochromes ethidium bromide and acridine orange. Expression of p53 was examined on HeLa cell line, by PCR with p53 wild-type primer. Expression of p21 was examined on HeLa cell line, by immunohistochemistry method. 5-fluorourasil was used as positive control in cytotoxic, apoptotic assay, and p53 expression. The result showed that calanone has cytotoxic activity on HeLa cell line, with IC50 = 22.887 mg/mL, caused cytotoxicity through apoptotic mechanism, increase p53 tumor suppressor gene expression, while the p21 expression test showed a negative result.   Keywords: Calanone, cytotoxic, HeLa cell line

Identification and Expression Analysis of CD73 Inhibitors in Cervical Cancer

2020 ◽  
Vol 16 ◽  
Author(s):  
Jamshed Iqbal ◽  
Ayesha Basharat ◽  
Sehrish Bano ◽  
Syed Mobasher Ali Abid ◽  
Julie Pelletier ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Cervical Cancer ◽  
Western Blot ◽  
Hela Cell ◽  
Cell Line ◽  
Cell Apoptosis ◽  
Immunofluorescence Staining ◽  
Cell Cycle Analysis ◽  
Hela Cell Line ◽  
Cell Line Hela

Aims: The present study was conducted to examine the inhibitory effects of synthesized sulfonylhydrazones on the expression of CD73 (ecto-5′-NT). Background: CD73 (ecto-5′-NT) represents the most significant class of ecto-nucleotidases which are mainly responsible for dephosphorylation of adenosine monophosphate to adenosine. Inhibition of CD73 played an important role in the treatment of cancer, autoimmune disorders, precancerous syndromes, and some other diseases associated with CD73 activity. Objective: Keeping in view the significance of CD73 inhibitor in the treatment of cervical cancer, a series of sulfonylhydrazones (3a-3i) derivatives synthesized from 3-formylchromones were evaluated. Methods: All sulfonylhydrazones (3a-3i) were evaluated for their inhibitory activity towards CD73 (ecto-5′-NT) by the malachite green assay and their cytotoxic effect was investigated on HeLa cell line using MTT assay. Secondly, most potent compound was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis. After that, CD73 mRNA and protein expression were analyzed by real-time PCR and Western blot. Results: Among all compounds, 3h, 3e, 3b, and 3c were found the most active against rat-ecto-5′-NT (CD73) enzyme with IC50 (µM) values of 0.70 ± 0.06 µM, 0.87 ± 0.05 µM, 0.39 ± 0.02 µM and 0.33 ± 0.03 µM, respectively. These derivatives were further evaluated for their cytotoxic potential against cancer cell line (HeLa). Compound 3h and 3c showed the cytotoxicity at IC50 value of 30.20 ± 3.11 µM and 86.02 ± 7.11 µM, respectively. Furthermore, compound 3h was selected for cell apoptosis, immunofluorescence staining and cell cycle analysis which showed promising apoptotic effect in HeLa cells. Additionally, compound 3h was further investigated for its effect on expression of CD73 using qRT-PCR and western blot. Conclusion: Among all synthesized compounds (3a-3i), Compound 3h (E)-N'-((6-ethyl-4-oxo-4H-chromen-3-yl) methylene)-4-methylbenzenesulfonohydrazide was identified as most potent compound. Additional expression studies conducted on HeLa cell line proved that this compound successfully decreased the expression level of CD73 and thus inhibiting the growth and proliferation of cancer cells.

Sign in / Sign up

Export Citation Format

Share Document