Development of drug laboratory methods and manufacture technology for specific diagnosis of hemorrhagic fever with renal syndrome

Dengue, Chikungunya and Zika are the vector-borne diseases that constitute a potential epidemiological risk due to the recent increase in cases, complications, and severity. The co-circulation of the three diseases is a matter of public health interest due to their transmission by the same vector as well as the increase in the number of cases of severe dengue hemorrhagic fever, post-chikungunya chronic joint disease and microcephaly related to Zika virus. Therefore, it is important to be familiar with the various clinical presentations and laboratory methods to make the differential diagnosis, start appropriate treatment, and prevent the associated complications. Bangladesh J Medicine July 2019; 30(2) : 83-92

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Novel Molecular Beacon Probe-Based Real-Time RT-PCR Assay for Diagnosis of Crimean-Congo Hemorrhagic Fever Encountered in India

BioMed Research International ◽

10.1155/2014/496219 ◽

2014 ◽

Vol 2014 ◽

pp. 1-4 ◽

Cited By ~ 6

Author(s):

Aman Kamboj ◽

Atul Kumar Pateriya ◽

Anamika Mishra ◽

Pradip Ranaware ◽

Diwakar D. Kulkarni ◽

...

Keyword(s):

Real Time ◽

Molecular Beacon ◽

Hemorrhagic Fever ◽

Taqman Assay ◽

Rt Pcr ◽

Pcr Assay ◽

Specific Diagnosis ◽

Crimean Congo Hemorrhagic Fever ◽

Hemorrhagic Fever Virus ◽

Routine Diagnosis

Crimean-Congo hemorrhagic fever (CCHF) is an emerging zoonotic disease in India and requires immediate detection of infection both for preventing further transmission and for controlling the infection. The present study describes development, optimization, and evaluation of a novel molecular beacon-based real-time RT-PCR assay for rapid, sensitive, and specific diagnosis of Crimean-Congo hemorrhagic fever virus (CCHFV). The developed assay was found to be a better alternative to the reported TaqMan assay for routine diagnosis of CCHF.

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A novel mu-capture enzyme-linked immunosorbent assay based on recombinant proteins for sensitive and specific diagnosis of hemorrhagic fever with renal syndrome.

Journal of Clinical Microbiology ◽

10.1128/jcm.31.5.1194-1199.1993 ◽

1993 ◽

Vol 31 (5) ◽

pp. 1194-1199 ◽

Cited By ~ 52

Author(s):

L G Zöller ◽

S Yang ◽

P Gött ◽

E K Bautz ◽

G Darai

Keyword(s):

Recombinant Proteins ◽

Immunosorbent Assay ◽

Hemorrhagic Fever ◽

Enzyme Linked Immunosorbent Assay ◽

Specific Diagnosis

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Novel one-step real-time RT-PCR assay for rapid and specific diagnosis of Crimean-Congo hemorrhagic fever encountered in the Balkans

Journal of Virological Methods ◽

10.1016/j.jviromet.2005.11.006 ◽

2006 ◽

Vol 133 (2) ◽

pp. 175-179 ◽

Cited By ~ 52

Author(s):

Darja Duh ◽

Ana Saksida ◽

Miroslav Petrovec ◽

Iusuf Dedushaj ◽

Tatjana Avšič-Županc

Keyword(s):

Real Time ◽

Hemorrhagic Fever ◽

Rt Pcr ◽

Pcr Assay ◽

Specific Diagnosis ◽

Crimean Congo Hemorrhagic Fever ◽

The Balkans ◽

One Step

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Tumor Diagnosis

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100053966 ◽

1982 ◽

Vol 40 ◽

pp. 262-265

Author(s):

Bruce Mackay

Keyword(s):

Electron Microscopy ◽

Transmission Electron Microscopy ◽

Differential Diagnosis ◽

Light Microscopy ◽

Clinical Examination ◽

Ultrastructural Study ◽

Diagnostic Procedures ◽

Specific Diagnosis ◽

Transmission Electron ◽

Microscopic Diagnosis

The broadest application of transmission electron microscopy (EM) in diagnostic medicine is the identification of tumors that cannot be classified by routine light microscopy. EM is useful in the evaluation of approximately 10% of human neoplasms, but the extent of its contribution varies considerably. It may provide a specific diagnosis that can not be reached by other means, but in contrast, the information obtained from ultrastructural study of some 10% of tumors does not significantly add to that available from light microscopy. Most cases fall somewhere between these two extremes: EM may correct a light microscopic diagnosis, or serve to narrow a differential diagnosis by excluding some of the possibilities considered by light microscopy. It is particularly important to correlate the EM findings with data from light microscopy, clinical examination, and other diagnostic procedures.

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The application of electron microscopy to diagnosis in gynecologic neoplasms and tumor-like conditions

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100110787 ◽

1984 ◽

Vol 42 ◽

pp. 102-105

Author(s):

Lawrence M. Roth

Keyword(s):

Electron Microscopy ◽

Reproductive Tract ◽

Malignant Tumors ◽

Frozen Section ◽

Cost Effective ◽

Female Reproductive Tract ◽

Ultrastructural Examination ◽

Specific Diagnosis ◽

Potential Value ◽

Gynecologic Neoplasms

The female reproductive tract may be the site of a wide variety of benign and malignant tumors, as well as non-neoplastic tumor-like conditions, most of which can be diagnosed by light microscopic examination including special stains and more recently immunoperoxidase techniques. Nevertheless there are situations where ultrastructural examination can contribute substantially to an accurate and specific diagnosis. It is my opinion that electron microscopy can be of greatest benefit and is most cost effective when applied in conjunction with other methodologies. Thus, I have developed an approach which has proved useful for me and may have benefit for others. In cases where it is deemed of potential value, glutaraldehyde-fixed material is obtained at the time of frozen section or otherwise at operation. Coordination with the gynecologic oncologist is required in the latter situation. This material is processed and blocked and is available if a future need arises.

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