scholarly journals Dilemma in laboratory diagnosis of Dengue, Chikungunya and Zika viruses

2019 ◽  
Vol 30 (2) ◽  
pp. 83-92 ◽  
Author(s):  
Mushtaque Ahmed ◽  
Nabeela Mahboob ◽  
Kazi Taib Mamun ◽  
Hasina Iqbal
Keyword(s):  
Hemorrhagic Fever ◽  
Laboratory Diagnosis ◽  
Joint Disease ◽  
Severe Dengue ◽  
Laboratory Methods ◽  
Vector Borne Diseases ◽  
Appropriate Treatment ◽  
Clinical Presentations ◽  
Epidemiological Risk ◽  
Vector Borne

Dengue, Chikungunya and Zika are the vector-borne diseases that constitute a potential epidemiological risk due to the recent increase in cases, complications, and severity. The co-circulation of the three diseases is a matter of public health interest due to their transmission by the same vector as well as the increase in the number of cases of severe dengue hemorrhagic fever, post-chikungunya chronic joint disease and microcephaly related to Zika virus. Therefore, it is important to be familiar with the various clinical presentations and laboratory methods to make the differential diagnosis, start appropriate treatment, and prevent the associated complications. Bangladesh J Medicine July 2019; 30(2) : 83-92

Vector-borne diseases in Iran: epidemiology and key challenges

2021 ◽  
Vol 16 (1) ◽  
pp. 51-69
Author(s):  
Najmeh Parhizgari ◽  
Norair Piazak ◽  
Ehsan Mostafavi
Keyword(s):  
Q Fever ◽  
Hemorrhagic Fever ◽  
Health Concern ◽  
Current Status ◽  
Vector Borne Diseases ◽  
High Prevalence ◽  
Dispersion Patterns ◽  
Monitoring And Surveillance ◽  
Vector Borne ◽  
Review Study

Vector-borne diseases have become a global health concern in recent decades as a result of global warming, globalization, growth in international trade and travel, use of insecticide and drug resistance. This review study addressed the key vector-borne diseases and their current status in Iran to emphasize the requirements for further research on vector-borne diseases. The dispersion patterns of these diseases differ in various regions. Some of them such as Crimean–Congo hemorrhagic fever, and Q fever are distributed all across Iran, whereas some others such as plague, leishmaniasis, tularemia, and malaria are restricted to specific areas. The high prevalence of vectors throughout the country necessitates enhancing the monitoring and surveillance of emerging and reemerging vector-borne diseases and their potential vectors.

scholarly journals New Real-Time PCRs to Differentiate Rickettsia spp. and Rickettsia conorii

Molecules ◽  
2020 ◽  
Vol 25 (19) ◽  
pp. 4431 ◽  
Author(s):  
Valeria Blanda ◽  
Rosalia D’Agostino ◽  
Elisabetta Giudice ◽  
Kety Randazzo ◽  
Francesco La Russa ◽  
...  
Keyword(s):  
Infectious Diseases ◽  
Real Time ◽  
Emerging Infectious Diseases ◽  
Laboratory Diagnosis ◽  
Diagnostic Methods ◽  
Rickettsia Conorii ◽  
Rickettsia Species ◽  
Vector Borne Diseases ◽  
Vector Borne ◽  
Dna Primers

Rickettsia species are an important cause of emerging infectious diseases in people and animals, and rickettsiosis is one of the oldest known vector-borne diseases. Laboratory diagnosis of Rickettsia is complex and time-consuming. This study was aimed at developing two quantitative real-time PCRs targeting ompB and ompA genes for the detection, respectively, of Rickettsia spp. and R. conorii DNA. Primers were designed following an analysis of Rickettsia gene sequences. The assays were optimized using SYBR Green and TaqMan methods and tested for sensitivity and specificity. This study allowed the development of powerful diagnostic methods, able to detect and quantify Rickettsia spp. DNA and differentiate R. conorii species.

scholarly journals Emerging and Re-emerging Vector-Borne Infectious Diseases and the Challenges for Control: A Review

2021 ◽  
Vol 9 ◽  
Author(s):  
Bayissa Chala ◽  
Feyissa Hamde
Keyword(s):  
Infectious Diseases ◽  
Public Health Problem ◽  
Hemorrhagic Fever ◽  
Emerging Diseases ◽  
West Nile Fever ◽  
Valley Fever ◽  
Vector Borne Diseases ◽  
Vector Borne ◽  
The Many ◽  
Epidemiological Profile

Vector-borne emerging and re-emerging diseases pose considerable public health problem worldwide. Some of these diseases are emerging and/or re-emerging at increasing rates and appeared in new regions in the past two decades. Studies emphasized that the interactions among pathogens, hosts, and the environment play a key role for the emergence or re-emergence of these diseases. Furthermore, social and demographic factors such as human population growth, urbanization, globalization, trade exchange and travel and close interactions with livestock have significantly been linked with the emergence and/or re-emergence of vector-borne diseases. Other studies emphasize the ongoing evolution of pathogens, proliferation of reservoir populations, and antimicrobial drug use to be the principal exacerbating forces for emergence and re-emergence of vector-borne infectious diseases. Still other studies equivocally claim that climate change has been associated with appearance and resurgence of vector-borne infectious diseases. Despite the fact that many important emerging and re-emerging vector-borne infectious diseases are becoming better controlled, our success in stopping the many new appearing and resurging vector-borne infectious diseases that may happen in the future seems to be uncertain. Hence, this paper reviews and synthesizes the existing literature to explore global patterns of emerging and re-emerging vector-borne infections and the challenges for their control. It also attempts to give insights to the epidemiological profile of major vector-borne diseases including Zika fever, dengue, West Nile fever, Crimean-Congo hemorrhagic fever, Chikungunya, Yellow fever, and Rift Valley fever.

scholarly journals Natural Infection of Aedes aegypti by Chikungunya and Dengue type 2 Virus in a Transition Area of North-Northeast Brazil

Viruses ◽  
2019 ◽  
Vol 11 (12) ◽  
pp. 1126 ◽  
Author(s):  
Carine Fortes Aragão ◽  
Valéria Cristina Soares Pinheiro ◽  
Joaquim Pinto Nunes Neto ◽  
Eliana Vieira Pinto da Silva ◽  
Glennda Juscely Galvão Pereira ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Asian American ◽  
Natural Infection ◽  
Rna Extraction ◽  
Hemorrhagic Fever ◽  
Severe Dengue ◽  
Northeast Brazil ◽  
Epidemiological Risk ◽  
Human Infections ◽  
Dengue Type 2 Virus

Dengue fever, chikungunya, and Zika are diseases caused by viruses transmitted by Aedes aegypti and Aedes albopictus. In Brazil, the number of human infections is high, but few studies are performed in mosquito vectors. This study aimed to investigate the presence of Zika, Dengue and Chikungunya viruses in Ae. aegypti and Ae. albopictus from the municipalities of Alto Alegre, Caxias, Codó, and São Mateus do Maranhão, located in the state of Maranhão, Northeast Brazil. The mosquitoes were collected with a mechanical aspirator, identified, triturated, and then submitted to RNA extraction and RT-qPCR. The positive samples were confirmed by virus isolation and genome sequencing. Three hundred and forty-eight Ae. aegypti (176 males and 172 females) and 12 Ae. albopictus (eight males and four females) were collected and tested. Ae. aegypti was the only vector positive in two municipalities—Codó, with detection of Chikungunya virus (CHIKV) belonging to the East-Central-South African genotype, and in Caxias, with detection of Dengue virus (DENV)-2 belonging to the Asian/American genotype. The detection of CHIKV and DENV-2 is evidence that those viruses are maintained in arthropod vectors, and shows the epidemiological risk in the area for chikungunya cases and a possible increase of severe dengue cases, associated with the occurrence of dengue hemorrhagic fever.

scholarly journals Young and Old Sugar Apple (Annona squamosa Linn) Leaf Extracts As an Aedes aegypti Larva Insecticide

2019 ◽  
Author(s):  
Abdul Khair ◽  
Noraida Noraida
Keyword(s):  
Aedes Aegypti ◽  
Leaf Extract ◽  
Hemorrhagic Fever ◽  
Control Group ◽  
Leaf Extracts ◽  
Control Group Design ◽  
Vector Borne Diseases ◽  
Apple Leaves ◽  
Significant Difference ◽  
Vector Borne

Vector-borne diseases are still a health problem in some tropical countries. One vector-borne disease is Dengue Hemorrhagic Fever (DHF). DHF never decreases and even tends to continue to increase, and many cause deaths in children, 90% of them attack children under 15 years. DHF cases in 2018 amounted to 65,602 cases, with 467 deaths (CFR = 0.71%). The behavior of holding water in various places such as tubs and tendons indirectly creates a breeding place for Aedes aegypti mosquitoes. The use of chemicals as insecticides can cause the death of non-target animals, environmental pollution, and the occurrence of vector resistance to insecticides. Therefore it is necessary to do other methods including the use of vegetable insecticides. Sugar apple leaves can be used to kill Aedes aegypti larvae. Sugar apple leaves will obtain throughout the year. The purpose of this study is to know the ability of young and old sugar apple leaves to kill Aedes aegypti larvae. This type of research is a posttest only control group design. The study sample was part of the Aedes aegypti larvae on the final instar III. The experiment to kill Aedes aegypti larvae use two types of sugar apple leaf extract, namely young and old sugar apple leaves with nine treatments four replications. Each treatment consisted of 25 larvae. The research results show There was no significant difference in the number of dead larvae using either extracts from young sugar apple leaves or old sugar apple leaves. LC90 of sugar apple leaf extract was between 0.05632 to 0.08324% and the effective residual age at LC90 (0.06568%) with the death of Aedes aegypti larvae was 92% over 24 hours (1 day).

scholarly journals LYME BORRELIOSIS: CLINICAL MANIFESTATIONS AND ADVANCES IN DIAGNOSIS AND CONTROL

2020 ◽  
Vol 8 (6) ◽  
pp. 721-729
Author(s):  
Sandip Kumar Khurana ◽  
◽  
Anju Sehrawat ◽  
Ruchi Tiwari ◽  
Khan Sharun ◽  
...  
Keyword(s):  
Lyme Disease ◽  
Early Stage ◽  
Erythema Migrans ◽  
Clinical Manifestations ◽  
Neurological Involvement ◽  
Vector Borne Diseases ◽  
Clinical Syndromes ◽  
Clinical Presentations ◽  
Vector Borne ◽  
And Control

Lyme disease or borreliosis is presumed one of the most significant vector-borne diseases globally. The disease is re-emerging in numerous parts of world. It has expanded dramatically in newer areas in recent decades. Lyme disease is caused by Borrelia burgdorferi yet additionally by other borrelial species, B. afzelii and B. garini which cause diverse clinical syndromes. Spatial distribution and clinical presentations differ depending on the causative species. Clinical manifestations of Lyme disease can be delineated in three stages. The first stage is presented in the form of erythema migrans at the site of tick bite. Early dispersed stage can lead to multiple lesions of erythema migrans, neuroborreliosis, lymphocytoma, arthritis or carditis. The manifestation at later stage shows acordermatitis chronica atrophicans, arthritis and neurological involvement. Diagnosis is challenging owing the several clinical presentations and could require multiple tests. The antibiotics that are currently under use, help in the clearance of bacteria from the affected host and stop further spread of the disease. Although several antibiotics are being used for Lyme disease, doxycycline is the widely used antimicrobial in early stage of the disease. Several attempts have been made to develop a vaccine against Lyme disease, however, none of them have been successfully marketed. The present review discusses clinical manifestations, and advances in diagnosis and control of Lyme disease.

scholarly journals THE EFFECT OF CLIMATE FACTORS FOR DENGUE HEMORRHAGIC FEVER IN BANJARMASIN CITY, SOUTH KALIMANTAN PROVINCE, INDONESIA, 2012-2016

2018 ◽  
Vol 4 (3) ◽  
pp. 121-128
Author(s):  
Nuning Irnawulan Ishak ◽  
Kasman Kasman
Keyword(s):  
Wind Speed ◽  
Path Analysis ◽  
Hemorrhagic Fever ◽  
P Value ◽  
Climate Factors ◽  
Climate Variations ◽  
National Data ◽  
Vector Borne Diseases ◽  
High Incidence ◽  
Vector Borne

Background: One of the factors that lead to high incidence of DHF is climate change.Objective: To analyze the effect of climate factors (temperature, humidity, wind speed, and rainfall) associated with DHF incidence in Banjarmasin City, 2012-2016.  Methods: We used the national data on annual reported incidence from Health Office of Banjarmasin City and climate variations from Meteorology Climatology and Geophysics Agency 2nd Class Climatology Station Syamsudin Noor Banjarmasin, January 2012-December 2016.  The analysis techniques using path analysis to explained the mechanism of causal relationships between variables.Results: The result showed the overall incidence of DHF in Banjarmasin City during 2012- 2016 was 243 cases, of DHF cases were fluctuates by the monthly trend, where the highest number of DHF cases in January to March, climate variation which occurred in Banjarmasin City period 2012-2016 included temperatures ranged from 25.8-28.7°C, humidity ranged from 65-88%, wind speed ranged from 4-6 knots and rainfall ranged from 0.0-546.7 mm, and the path analysis showed that rainfall variable (X4) was the only variable which positively effected to DHF incidence variable (Y) equal to 0.613 unit (Y = 0.613 X4) (p value = 0.002).Conclusion: Climate information can used as a precautionary signal through early warming of the readiness in facing the outbreaks of vector borne diseases so that further efforts in environmental management by manipulation method and environmental modification. 

scholarly journals Seroprevalence against Rickettsia and Borrelia Species in Patients with Uveitis: A Prospective Survey

2017 ◽  
Vol 2017 ◽  
pp. 1-10
Author(s):  
Kim B. Madsen ◽  
Katarina Wallménius ◽  
Åke Fridman ◽  
Carl Påhlson ◽  
Kenneth Nilsson
Keyword(s):  
Medical Records ◽  
Blood Donors ◽  
Control Group ◽  
Previous Exposure ◽  
Prospective Survey ◽  
Vector Borne Diseases ◽  
Exposed Population ◽  
Epidemiological Risk ◽  
Epidemiological Risk Factor ◽  
Vector Borne

Vector-borne diseases such as Lyme borreliosis and rickettsioses have been associated with ocular inflammation. Our aim was to study patients with diagnosed uveitis to evaluate serological signs of infection or exposure to these tick-borne agents. Forty-eight patients were prospectively examined with serology together with medical records and a questionnaire concerning previous exposure, diseases, and treatments. Seven patients (14.6%) showed seroconversion to Rickettsia spp. between acute and convalescent phase sera, which provides support for a positive Rickettsia diagnosis according to guidelines. The specificity was confirmed by Western blot. Additional 28 patients had stationary titres of which eight (16.6%) had 1 : 256 or higher titre in the first serum, and another 13 patients were seronegative. No epidemiological risk factor or marker could be identified. For Borrelia, only three patients showed moderate IgG titres. A control group of 100 blood donors, 60 patients with rheumatic disease, and 56 patients seeking medical care were tested of which 2.0–7.1% showed low anti-Rickettsia titres and 3.0–8.3% anti-Borrelia titres. The findings are indicative for an association between infection or exposure to Rickettsia spp. and uveitis with a seropositivity among patients with recurrent uveitis in concordance with the spread of rickettsial exposure in a tick-exposed population.

scholarly journals Bartonella spp. as emerging human pathogens.

1997 ◽  
Vol 10 (2) ◽  
pp. 203-219 ◽  
Author(s):  
B E Anderson ◽  
M A Neuman
Keyword(s):  
Opportunistic Infections ◽  
Systemic Disease ◽  
Laboratory Diagnosis ◽  
Current Status ◽  
Cat Scratch Disease ◽  
Human Pathogens ◽  
Laboratory Methods ◽  
Bacillary Angiomatosis ◽  
Clinical Presentations ◽  
Life Threatening

Members of the genus Bartonella (formerly Rochalimaea) were virtually unknown to modern-day clinicians and microbiologists until they were associated with opportunistic infections in AIDS patients about 6 years ago. Since that time, Bartonella species have been associated with cat scratch disease, bacillary angiomatosis, and a variety of other disease syndromes. Clinical presentation of infection with Bartonella ranges from a relatively mild lymphadenopathy with few other symptoms, seen in cat scratch disease, to life-threatening systemic disease in the immunocompromised patient. In some individuals, infection manifests as lesions that exhibit proliferation of endothelial cells and neovascularization, a pathogenic process unique to this genus of bacteria. As the spectrum of disease attributed to Bartonella is further defined, the need for reliable laboratory methods to diagnose infections caused by these unique organisms also increases. A brief summary of the clinical presentations associated with Bartonella infections is presented, and the current status of laboratory diagnosis and identification of these organisms is reviewed.

scholarly journals Surveys of Canine Filarioids In Nigeria: The Path Travelled and the Way Forward

2021 ◽  
Vol 41 (3) ◽  
Author(s):  
Kamani Joshua ◽  
Javier Gonzlez-Miguel
Keyword(s):  
Risk Factors ◽  
Dirofilaria Immitis ◽  
Human Infection ◽  
Diagnostic Methods ◽  
Zoonotic Potential ◽  
Laboratory Methods ◽  
Vector Borne Diseases ◽  
Acanthocheilonema Reconditum ◽  
Vector Borne ◽  
Canine Filarioids

Filarioid worms infecting dogs have recently received increased attention globally because of their zoonotic potential. In Africa and, particularly, in Nigeria, however, where there is preponderance of the risk factors for vector-borne diseases transmission, there are few reports of the disease in the canid and felid definitive hosts, the wild/domestic reservoirs and humans. Thus, the epidemiology of the disease in Nigeria remains sketchy and needs to be investigated. A retrospective analysis of reported canine filarioids in Nigeria was undertaken with the view to highlight what has been done and reported, existing gaps in knowledge, what needs to be done to bridge the gap and possibly how it could be done. Thirteen published works on canine filarioids using classical laboratory methods in Nigeria, reported the finding of Dirofilaria immitis (0.4–15.1%), Dirofilaria repens (0.1–9.4%), Acanthocheilonema reconditum (0.4–9.2%) and a case of A. dracunculoides in an unspecified dog population. In most instances, the species identification of the filarioids reported was not conclusive due to limitation of the diagnostic methods employed. No human infection due to any zoonotic canine filarioid has been reported in Nigeria. Suggestions for prospective filarioids research in Nigeria were made. Keywords: Filarioids, dog, zoonosis, diagnosis, Nigeria

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