Antiviral Activity of Extracts from the Daedaleopsis confragosa F-1368 Basidiomycete

2021 ◽  
Vol 37 (1) ◽  
pp. 21-25
Author(s):  
T.N. Ilyicheva ◽  
E.D. Danilenko ◽  
L.R. Lebedev
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Pandemic Influenza ◽  
Culture Medium ◽  
Antiviral Agents ◽  
Hydrolytic Enzymes ◽  
Therapeutic Index ◽  
The State ◽  
Influenza Virus A ◽  
State Assignment

The antiviral activity of extracts of the basidial fungus Daedaleopsis confragosa (Bolton) J. Schröt strain F-1368 cultivated under the laboratory conditions has been studied. It was shown that the extracts obtained from 1 L of the culture contained proteins and polysaccharides in the amount of 71±5 and 413±15 mg/L of culture, respectively. Antiviral activity (ED50) against the pandemic influenza virus A/California/07/09 (H1N1)pdm09 was observed at extract concentrations in the range of 0.02-0.12 mg/mL depending on the culture medium. The therapeutic index (CC50/ED50) was at the level of 14-17, which makes it possible to recommend D. confragosa extracts for the development of antiviral agents. It was shown that treatment with hydrolytic enzymes (trypsin, zymolyase) leads to an increase in cytotoxicity and a decrease in the antiviral activity of the extracts. Daedaleopsis confragosa, basidomycetes, extracts, antiviral activity, influenza virus The study was carried out according to the state assignment to FBRI SRC VB «Vector», Rospotrebnadzor, Koltsovo.

scholarly journals Synthesis and Antiviral Activity of Quercetin Brominated Derivatives

2015 ◽  
Vol 10 (9) ◽  
pp. 1934578X1501000 ◽  
Author(s):  
Elza Karimova ◽  
Lidia Baltina ◽  
Leonid Spirikhin ◽  
Tagir Gabbasov ◽  
Yana Orshanskaya ◽  
...  
Keyword(s):  
Cell Culture ◽  
Influenza Virus ◽  
Acetic Acid ◽  
Antiviral Activity ◽  
Pandemic Influenza ◽  
Mdck Cell ◽  
Inhibitory Activity ◽  
Glacial Acetic Acid ◽  
Absolute Ethanol ◽  
Influenza Virus A

Reaction of quercetin (QR) (1) with bromine under various conditions was studied. Interaction of QR with 2–3 equiv. of bromine in glacial acetic acid at 35–40°C for 2–4 h and 20–22°C for 24 h led to the formation of QR 6,8-dibromide (2) (52–54% yields, 96–98% purity by HPLC). Interaction of QR with 2–5 equiv. bromine in absolute ethanol at 0–5°C and 20–22°C for 24 h led to the formation of 3- O-ethyl-QR-2,3,6,8,5′-pentabromide (3) (95–97% purity by HPLC) the output of which depends on the quantity of bromine. It was shown in MDCK cell culture that compound 2 exhibits a moderate inhibitory activity against pandemic influenza virus A/H1N1/pdm09 (EC50 6.0 μg/mL, CTD50 97.7 μg/mL, SI 16). Compound 3 was inactive.

Antiviral Activity of Anaferon (Pediatric Formulation) in Mice Infected with Pandemic Influenza Virus A(H1N1/09)

2010 ◽  
Vol 149 (5) ◽  
pp. 612-614 ◽  
Author(s):  
L. N. Shishkina ◽  
M. O. Skarnovich ◽  
A. S. Kabanov ◽  
A. A. Sergeev ◽  
S. E. Olkin ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Pandemic Influenza ◽  
Influenza Virus A ◽  
Pediatric Formulation

scholarly journals Synthesis and Antiviral Evaluation of Nucleoside Analogues Bearing One Pyrimidine Moiety and Two D-Ribofuranosyl Residues

Molecules ◽  
2021 ◽  
Vol 26 (12) ◽  
pp. 3678
Author(s):  
Olga V. Andreeva ◽  
Bulat F. Garifullin ◽  
Vladimir V. Zarubaev ◽  
Alexander V. Slita ◽  
Iana L. Yesaulkova ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Theoretical Calculations ◽  
Nucleoside Analogs ◽  
Coxsackievirus B3 ◽  
Nucleoside Analogues ◽  
Moderate Activity ◽  
Coat Proteins ◽  
Influenza Virus A ◽  
Ic50 Values

A series of 1,2,3-triazolyl nucleoside analogues in which 1,2,3-triazol-4-yl-β-d-ribofuranosyl fragments are attached via polymethylene linkers to both nitrogen atoms of the heterocycle moiety (uracil, 6-methyluracil, thymine, quinazoline-2,4-dione, alloxazine) or to the C-5 and N-3 atoms of the 6-methyluracil moiety was synthesized. All compounds synthesized were evaluated for antiviral activity against influenza virus A/PR/8/34/(H1N1) and coxsackievirus B3. Antiviral assays revealed three compounds, 2i, 5i, 11c, which showed moderate activity against influenza virus A H1N1 with IC50 values of 57.5 µM, 24.3 µM, and 29.2 µM, respectively. In the first two nucleoside analogues, 1,2,3-triazol-4-yl-β-d-ribofuranosyl fragments are attached via butylene linkers to N-1 and N-3 atoms of the heterocycle moiety (6-methyluracil and alloxazine, respectively). In nucleoside analogue 11c, two 1,2,3-triazol-4-yl-2′,3′,5′-tri-O-acetyl-β-d-ribofuranose fragments are attached via propylene linkers to the C-5 and N-3 atoms of the 6-methyluracil moiety. Almost all synthesized 1,2,3-triazolyl nucleoside analogues showed no antiviral activity against the coxsackie B3 virus. Two exceptions are 1,2,3-triazolyl nucleoside analogs 2f and 5f, in which 1,2,3-triazol-4-yl-2′,3′,5′-tri-O-acetyl-β-d-ribofuranose fragments are attached to the C-5 and N-3 atoms of the heterocycle moiety (6-methyluracil and alloxazine respectively). These compounds exhibited high antiviral potency against the coxsackie B3 virus with IC50 values of 12.4 and 11.3 µM, respectively, although both were inactive against influenza virus A H1N1. According to theoretical calculations, the antiviral activity of the 1,2,3-triazolyl nucleoside analogues 2i, 5i, and 11c against the H1N1 (A/PR/8/34) influenza virus can be explained by their influence on the functioning of the polymerase acidic protein (PA) of RNA-dependent RNA polymerase (RdRp). As to the antiviral activity of nucleoside analogs 2f and 5f against coxsackievirus B3, it can be explained by their interaction with the coat proteins VP1 and VP2.

scholarly journals Lack of evidence for pre-symptomatic transmission of pandemic influenza virus A(H1N1) 2009 in an outbreak among teenagers; Germany, 2009

2011 ◽  
Vol 5 (6) ◽  
pp. e499-e503 ◽  
Author(s):  
Julia Hermes ◽  
Helen Bernard ◽  
Udo Buchholz ◽  
Michaela Spackova ◽  
Johann Löw ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Pandemic Influenza ◽  
Influenza Virus A

scholarly journals Screening and Purification of NanB Sialidase From Pasteurella Multocida With Activity in Hydrolyzing Sialic Acid Neu5Acα(2-6)Gal

2021 ◽  
Author(s):  
Christian Marco Hadi Nugroho ◽  
Ryan Septa Kurnia ◽  
Simson Tarigan ◽  
Otto Sahat Martua Silaen ◽  
Silvia Tri Widyaningtyas ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Silico ◽  
Pasteurella Multocida ◽  
Antiviral Agents ◽  
Influenza Virus Infection ◽  
Ligand Complex ◽  
In Silico Studies

Abstract Study on sialidases as antiviral agents has been widely performed, but many types of sialidase had not been tested for their antiviral activity. One of such sialidase is the NanB sialidase of Pasteurella multocida, which has never been isolated for further study. In this study, the activity of NanB sialidase was investigated in silico by docking the NanB sialidase of Pasteurella multocida to the Neu5Acα(2-6)Gal ligand. Additionally, some local isolates of Pasteurella multocida, which had the NanB gene were screened, and the proteins were isolated for further testing regarding their activity in hydrolyzing Neu5Acα(2-6)Gal. In silico studies showed that the NanB sialidase possesses an exceptional affinity towards forming a protein-ligand complex with Neu5Acα(2-6)Gal. This was further confirmed by showing that a dose of 0.258 U/ml (100%) NanB sialidase of Pasteurella multocida B018 can hydrolyze up to 44.28% of Neu5Acα(2-6)Gal in chicken red blood cells and 81.95% in rabbit red blood cells. This study suggested that the NanB sialidase of Pasteurella multocida B018 has a potent antiviral activity that can inhibit avian influenza virus infection.

scholarly journals Prevention of nosocomial transmission of swine-origin pandemic influenza virus A/H1N1 by infection control bundle

2010 ◽  
Vol 74 (3) ◽  
pp. 271-277 ◽  
Author(s):  
V.C.C. Cheng ◽  
J.W.M. Tai ◽  
L.M.W. Wong ◽  
J.F.W. Chan ◽  
I.W.S. Li ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Infection Control ◽  
Pandemic Influenza ◽  
Nosocomial Transmission ◽  
Influenza Virus A

Genetic variability of isolates of pandemic influenza virus A H1N1 isolated in Russia in 2009

2011 ◽  
Vol 26 (4) ◽  
pp. 166-173
Author(s):  
A. N. Shikov ◽  
A. O. Sementsova ◽  
O. K. Demina ◽  
A. A. Sergeev ◽  
S. A. Berillo ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Genetic Variability ◽  
Pandemic Influenza ◽  
Influenza Virus A

scholarly journals Screening and Purification of NanB Sialidase from Pasteurella Multocida with Activity in Hydrolyzing Sialic Acid Neu5Acα(2-6)Gal

2021 ◽  
Author(s):  
Christian Marco Hadi Nugroho ◽  
Ryan Septa Kurnia ◽  
Simson Tarigan ◽  
Otto Sahat Martua Silaen ◽  
Silvia Tri Widyaningtyas ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Antiviral Activity ◽  
Red Blood Cells ◽  
Blood Cells ◽  
In Silico ◽  
Pasteurella Multocida ◽  
Antiviral Agents ◽  
Influenza Virus Infection ◽  
Ligand Complex ◽  
In Silico Studies

Abstract Study on sialidases as antiviral agents has been widely performed, but many types of sialidase had not been tested for their antiviral activity. One of such sialidase is the NanB sialidase of Pasteurella multocida, which has never been isolated for further study. In this study, the activity of NanB sialidase was investigated in silico by docking the NanB sialidase of Pasteurella multocida to the Neu5Acα(2-6)Gal ligand. Additionally, some local isolates of Pasteurella multocida, which had the NanB gene were screened, and the proteins were isolated for further testing regarding their activity in hydrolyzing Neu5Acα(2-6)Gal. In silico studies showed that the NanB sialidase possesses an exceptional affinity towards forming a protein-ligand complex with Neu5Acα(2-6)Gal. This was further confirmed by showing that a dose of 0.258 U/ml (100%) NanB sialidase of Pasteurella multocida B018 can hydrolyze up to 44.28% of Neu5Acα(2-6)Gal in chicken red blood cells and 81.95% in rabbit red blood cells. This study suggested that the NanB sialidase of Pasteurella multocida B018 has a potent antiviral activity that can inhibit avian influenza virus infection.

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